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Book Characterization and Genetic Analysis of a Newly isolated Crisp like Mutant and an Osmotic sensitive Mutant of Neurospora Crassa

Download or read book Characterization and Genetic Analysis of a Newly isolated Crisp like Mutant and an Osmotic sensitive Mutant of Neurospora Crassa written by Charlene Renee Jackson and published by . This book was released on 1992 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Genetic Analysis Microscopy and Characterization of a Colonial Pigmentation Mutant of Neurospora Crassa

Download or read book Genetic Analysis Microscopy and Characterization of a Colonial Pigmentation Mutant of Neurospora Crassa written by Charlotte H. Harris and published by . This book was released on 1990 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Genetic Analysis and Characterization of a New Osmotic sensitive Protoperithecial Mutant of Neurospora Crassa

Download or read book Genetic Analysis and Characterization of a New Osmotic sensitive Protoperithecial Mutant of Neurospora Crassa written by Stephanie Paige Stephens and published by . This book was released on 1994 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Genetic Analysis and Characterization of SS 788 and SS 1044 New Osmotic sensitive Mutants of Neurospora Crassa

Download or read book Genetic Analysis and Characterization of SS 788 and SS 1044 New Osmotic sensitive Mutants of Neurospora Crassa written by Scott McCollum Buntin and published by . This book was released on 1995 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Isolation and Characterization of Neurospora Cell Wall Biosynthesis and Anastomosis Defective Mutants

Download or read book Isolation and Characterization of Neurospora Cell Wall Biosynthesis and Anastomosis Defective Mutants written by Mash'el Aldabbous and published by . This book was released on 2009 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell-cell fusion is a highly regulated process in which individual cells merge and their cytosolic contents become mixed. To obtain a better understanding of this fundamental process, we have been studying the process of cell fusion (anastomosis) in the filamentous fungus Neurospora crassa . The major goal of the research presented in this thesis was to identify proteins that mediate the steps involved in the process of anastomosis. To achieve this goal, a strategy was utilized that involved isolation of Neurospora mutants specifically affected in the process of anastomosis, characterization of the mutants, and the identification of the mutated genes. Initially, thirty-four mutants affected in the process of anastomosis were isolated in a large-scale random mutagenesis experiment, using a screening assay to identify mutants unable to form heterokaryons. Heterokaryons are cells containing two different types of nuclei, and are formed by anastomosis. The mutants were characterized and some of the mutated genes were mapped onto the Neurospora genetic map. Sixteen of those mutants showed colonial growth morphology. Most of the other mutants showed reduced growth rates and altered general morphology, in addition to the defect in anastomosis. The work in chapter two of this thesis details the identification of the gpip-1, gpip-2, gpip-3, and gpit-1 genes, which encode components of the biosynthetic complexes involved in the formation of the glycosylphosphatidylinositol (GPI) anchor and its addition to select cell wall glycoproteins. The MSA-7 mutant, which was among the anastomosis mutants identified in the mutant isolation procedure, played a critical role in the work described in chapter two. Characterization of the MSA-7 mutant showed that it contained a mutation in the gpip-1 gene, which encodes a subunit of the glycosylphosphotidylinositol anchor phosphoethanolamine transferase. This complex mediates the addition of phosphoethanolamine to the GPI anchor, a key step in the synthesis of the GPI anchor. The identification of the gpip-1 gene as being essential for normal cell wall biogenesis and anastomosis demonstrated the importance of GPI-anchored proteins for these processes. Based on the importance of GPI-anchoring for anastomosis, an analysis of the sequenced Neurospora genome was carried out, and additional genes involved in GPI-anchor biogenesis were identified. Mutational analysis of the gpip-2, gpip-3, and gpit-1 genes demonstrated that these genes were also involved in the synthesis of the GPI-anchor. The work in chapter three describes the isolation and characterization of three additional genes, rcm-1, rco-1 and ham-5. These three genes were shown to be required for anastomosis. In addition to the defect in anastomosis, these mutants show altered morphology, slow growth rates, and are defective in asexual and sexual development. We also show that these three genes are needed to produce specialized cells called conidial anastomosis tubes (CATs), which function in cell-cell fusion events between germinating conidia (asexual spores). The rcm-1 and rco-1 genes are homologs of the Saccharomyces cerevisiae genes SSN6 and TUP1 genes, respectively. SSN6 and TUP1 have been shown to form a dimeric transcription factor that plays an important role in regulating the growth and developmental activity of the yeast cell. The research in chapter three shows that the rcm-1 and rco-1 genes are needed to regulate growth and development in Neurospora. Overall, the results of this doctoral work demonstrated that GPI-anchored cell wall proteins play an important role in the formation of the cell wall and in anastomosis. GPI-anchored cell wall proteins function as structural elements of the cell wall and mediate cross-linking between the cell wall components. Mutations that disrupt the formation of a normal cell wall render the fungus unable to participate in anastomosis. The work also demonstrated, for the first time, that the rcm-1 and rco-1 genes were required for cell fusion events. Lastly, this work is the first to indicate that the newly identified ham-5 gene, encoding a cytosolic protein with an N terminal WD40 domain, is required for anastomosis. (Abstract shortened by UMI.).

Book Characterization Genetic Analysis and Reversion of a Fungicide resistant Mutant of Neurospora Crassa

Download or read book Characterization Genetic Analysis and Reversion of a Fungicide resistant Mutant of Neurospora Crassa written by Regan Matteil Challinor and published by . This book was released on 1996 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: