Download or read book Characterization and Functionalization of Substrates and Nanoparticles for Detection of Surface Binding Events of Biomolecules written by Selina Moses and published by . This book was released on 2005 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: microarrays, DNA hybridization, nanoparticles, photoelectrochemistry.
Download or read book Characterization and Functionalization of Substrates and Nanoparticles for Detection of Surface Binding Events of Biomolecules written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation mainly focuses on detection of biomolecules on metal and metal-oxide surfaces by photoelectrochemistry. Characterization of DNA monolayers on the surface of gold was studied in detail by X-ray photoelectron spectroscopy (XPS), Polarization Modulated Infrared reflectance absorbance Spectroscopy (PMIRRAS) and FTIR. An electrochemical technique Chronocoulometry (CC) quantified the surface coverage of ssDNA on gold using a redox label. The issues that affected the hybridization efficiency of DNA on the gold surface were analyzed and parameters for optimum hybridization were established by the same techniques used to detect ssDNA on the surface. Though gold is a well-known surface, the thiol chemistry, which is the basis of attaching DNA on the surface, is not robust and reproducible due to the desorption of probes from the surface during hybridization, which involves elevating the temperature. Hybridization yield on gold surfaces could not be determined accurately as the desorption of probe from the surface was a competing phenomenon during hybridization event. As a result, new substrates were analyzed and indium-doped tin oxide (ITO) was found to be a very useful substrate because of the ease with which it can be modified with COOH, Phosphates, Phosphonates and silanes. In addition, the physical properties of ITO make it an attractive candidate to use in creating bioarrays. It is highly conductive and tansparent in the visible region and reflective in the IR. Compared to the thiol chemistry on gold the phosphates are much stronger and resistant to elevated temperatures used for hybridization. ITO electrodes can sustain relatively high potentials required to oxidize gold nanoparticles used as labels to detect hybridization. All these properties can be utilized to characterize ssDNA modified ITO surfaces and surface DNA hybridization either by optical or electrical methods. 12-Phosphonododecanoic acid (12-PDA) formed a well-ordered monolayer on the ITO.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2006 with total page 886 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functionalization and Characterization of Nanostructured Materials for Sensing Applications written by Stacey Lynne Dean and published by . This book was released on 2011 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanobiosensors written by Alexandru Grumezescu and published by Academic Press. This book was released on 2016-09-28 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanobiosensors: Nanotechnology in the Agri-Food Industry, Volume 8, provides the latest information on the increasing demand for robust, rapid, inexpensive, and safe alternative technologies that monitor, test, and detect harmful or potentially dangerous foods. Due to their high sensitivity and selectivity, nanobiosensors have attracted attention for their use in monitoring not only biological contaminants in food, but also potential chemical and physical hazards. This book offers a broad overview regarding the current progress made in the field of nanosensors, including cutting-edge technological progress and the impact of these devices on the food industry. Special attention is given to the detection of microbial contaminants and harmful metabolotes, such as toxins and hormones, which have a great impact on both humans and animal health and feed. - Includes the most up-to-date information on nanoparticles based biosensors and quantum dots for biological detection - Provides application methods and techniques for research analysis for bacteriological detection and food testing - Presents studies using analytical tools to improve food safety and quality analysis
Download or read book Detection of Small Biomolecules by Plasmonic Nanoparticles LSPR sensing written by Jacqueline Jatschka and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metal nanostructures with their attractive optical properties are of great interest in the field of biosensing. In this thesis, a label-free online detection of biomolecules with immobilized nanoparticles in a microfluidic chamber was realized. Two label-free sensor arrangements based on densely adsorbed (LSPR) and single nanostructures (sLSPR) were combined with a microfluidic system and compared to a commercial available system based on a gold layer (SPR). The surface sensitivity of the three sensing systems were investigated by sequential deposition of charged polyelectrolyte (PEL) layers and protein multilayers. It was even possible to detect nm thin layers online on an area smaller than 0.02μm2 on a single 80 nm spherical nanoparticle. The measurements confirmed that the LSPR signal has a non-linear dependency on the number of deposited layer in opposite to the SPR system, that the signal change is higher compared to the SPR system and that the signal depends on the size of the nanoparticle and the plasmon mode. The real-time detection of DNA-DNA binding was realized on 80 nm spherical gold nanoparticles (LSPR setup) in a microfluidic chamber without labeling. Two DNA sequences were successfully adsorbed covalently on the nanoparticles' surfaces and the subsequent hybridization was traceable within 5 min at room temperature. The additional application of non-target DNA verified the specific binding between the capture and target DNA and the binding process was repeatable by a chemical denaturation agent. Investigations concerning the buffer during biofunctionalization, the pre-treatment of the substrates with plasma and the coadsorption of mercaptohexanol resulted in an enhanced capture DNA adsorption and availability for target DNA hybridization.
Download or read book Nanoplasmonic Sensors written by Alexandre Dmitriev and published by Springer Science & Business Media. This book was released on 2012-07-31 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compendium of the finest research in nanoplasmonic sensing done around the world in the last decade. It describes basic theoretical considerations of nanoplasmons in the dielectric environment, gives examples of the multitude of applications of nanoplasmonics in biomedical and chemical sensing, and provides an overview of future trends in optical and non-optical nanoplasmonic sensing. Specifically, readers are guided through both the fundamentals and the latest research in the two major fields nanoplasmonic sensing is applied to – bio- and chemo-sensing – then given the state-of-the-art recipes used in nanoplasmonic sensing research.
Download or read book Nanoparticles in Analytical and Medical Devices written by Fang Gang and published by Elsevier. This book was released on 2020-09-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoparticles in Analytical and Medical Devices presents the latest information on the use of nanoparticles for a diverse range of analytical and medical applications. Covers basic principles, proper use of nanoparticles in analytical and medical applications, and recent progress in the field. This comprehensive reference helps readers grasp the full potential of nanoparticles in their analytical research or medical practice. Chapters on cutting-edge topics bring readers up to date on the latest research and usage of nanoparticles, and a chapter on commercially available devices that utilize nanoparticles guides readers in overcoming issues with marketing biodevices. - Synthesizes nanoparticle conjugation and other critical methods - Covers nanoparticles in analytical methods and real analytical devices currently used in the medical field - Provides useful new information not covered in the current literature in chapters on surface chemical functionalization for bio-immobilization and nanoparticle production from natural sources
Download or read book One Dimensional Nanostructures written by Zhiming M Wang and published by Springer Science & Business Media. This book was released on 2008-07-20 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: One-dimensional (1D) nanostructures, including nanowires, nanotubes and quantum wires, have been regarded as the most promising building blocks for nanoscale electronic and optoelectronic devices. This book presents exciting, state-of-the-art developments in synthesis and properties of 1D nanostructures with many kinds of morphologies and compositions as well as their considerable impact on spintronics, information storage, and the design of field-effect transistors.
Download or read book Modern Introduction to Surface Plasmons written by Dror Sarid and published by Cambridge University Press. This book was released on 2010-05-06 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces graduate students in physics, optics, materials science and electrical engineering to surface plasmons, and applications of surface plasmon physics.
Download or read book Nanoscale Sensors written by Shibin Li and published by Springer Science & Business Media. This book was released on 2014-01-07 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive introduction to nanoscale materials for sensor applications, with a focus on connecting the fundamental laws of physics and the chemistry of materials with device design. Nanoscale sensors can be used for a wide variety of applications, including the detection of gases, optical signals, and mechanical strain, and can meet the need to detect and quantify the presence of gaseous pollutants or other dangerous substances in the environment. Gas sensors have found various applications in our daily lives and in industry. Semiconductive oxides, including SnO2, ZnO, Fe2O3, and In2O3, are promising candidates for gas sensor applications. Carbon nanomaterials are becoming increasingly available as “off-the-shelf” components, and this makes nanotechnology more exciting and approachable than ever before. Nano-wire based field- effect transistor biosensors have also received much attention in recent years as a way to achieve ultra-sensitive and label-free sensing of molecules of biological interest. A diverse array of semiconductor-based nanostructures has been synthesized for use as a photoelectrochemical sensor or biosensor in the detection of low concentrations of analytes. A novel acoustic sensor for structural health monitoring (SHM) that utilizes lead zirconate titanate (PZT) nano- active fiber composites (NAFCs) is described as well.
Download or read book General Methods in Biomarker Research and their Applications written by Victor R. Preedy and published by Springer. This book was released on 2015-08-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade there has been a major sea change in the way disease is diagnosed and investigated due to the advent of high throughput technologies, such as microarrays, lab on a chip, proteomics, genomics, lipomics, metabolomics etc. These advances have enabled the discovery of new and novel markers of disease relating to autoimmune disorders, cancers, endocrine diseases, genetic disorders, sensory damage, intestinal diseases etc. In many instances these developments have gone hand in hand with the discovery of biomarkers elucidated via traditional or conventional methods, such as histopathology or clinical biochemistry. Together with microprocessor-based data analysis, advanced statistics and bioinformatics these markers have been used to identify individuals with active disease or pathology as well as those who are refractory or have distinguishing pathologies. New analytical methods that have been used to identify markers of disease and is suggested that there may be as many as 40 different platforms. Unfortunately techniques and methods have not been readily transferable to other disease states and sometimes diagnosis still relies on single analytes rather than a cohort of markers. There is thus a demand for a comprehensive and focused evidenced-based text and scientific literature that addresses these issues. Hence the formulation of Biomarkers in Disease. The series covers a wide number of areas including for example, nutrition, cancer, endocrinology, cardiology, addictions, immunology, birth defects, genetics and so on. The chapters are written by national or international experts and specialists.
Download or read book Surface Plasmon Resonance in Bioanalysis written by and published by Elsevier. This book was released on 2021-11-11 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface Plasmon Resonance in Bioanalysis, Volume 95 in the Comprehensive Analytical Chemistry series, contains a wide range of topics on the applications and new advances of surface plasmon resonance (SPR) in bioanalysis, including Surface plasmon resonance microscopy for single-cell based drug screening, Phase-Sensitive Surface Plasmon Resonance Sensors for Highly Sensitive Bioanalysis, SPR coupled to ambient mass spectrometry, Surface Plasmon Resonance Microscopy for activity detection and imaging of single cells, SPR for water pollutant detection and biofouling control, SPR imaging for cellular analysis and detection, Progress in detection of surface palsmon resonance for biorefinery technology, and more. Additional chapters cover Long-range surface plasmon resonance and its biological sensing applications and Critical issues in clinical and biomedical applications of Surface Plasmon Resonance sensing. - Provides updates on the latest applications of SPR microscopy in cell analysis - Covers the latest design in SPR sensing for highly sensitive bioanalysis - Presents the critical issues in clinical and biomedical applications of SPR
Download or read book Handbook of Surface Plasmon Resonance written by Richard B. M. Schasfoort and published by Royal Society of Chemistry. This book was released on 2017-05-30 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy
Download or read book Chemical and Biochemical Applications written by Pierre Laszlo and published by Elsevier. This book was released on 2012-12-02 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR of Newly Accessible Nuclei, Volume 1: Chemical and Biochemical Applications is a 10-chapter text that explores the properties, advantages, developments, and chemical and biochemical applications of NMR technique. This book describes first the operation of an NMR spectrometer under its two aspects, namely, the instrumental and the computational aspects. The next chapters are devoted to some of the most important pulse sequences. The discussion then shifts to the various factors determining the position of the observed absorption and those responsible for the various relaxation processes. The last chapters deal with the specific applications of NMR, including in cation salvation, calcium-binding proteins, polyelectrolyte systems, halogens, and antibiotic ionophores. This book is of value to inorganic and analytical chemists, and biophysicists.
