Download or read book Characterisation of Two Rhoptry Proteins from the Malaria Parasite Plasmodium Falciparum written by Lev M. Kats and published by . This book was released on 2008 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of Two Novel Plasmodium Falciparum Rhoptry Neck Proteins written by Nicholas Proellocks and published by . This book was released on 2009 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: PfRON6 and Pf34 are expressed late in the life RBC life cycle consistent with a potential role in the invasion process. Indirect immunofluoresence assays have shown that Pf34 and PfRON6 are both found in the merozoite apex region and specifically localise to the rhoptry neck region. This localisation was confirmed by immunoelectron microscopy for PfRON6. To date Pf34 is the only GPI-anchored protein found in the rhoptry neck, and is also associated with parasite-derived detergent-resistant microdomains (DRMs). Our transfection experiments indicate that the gene that encodes PfRON6 is refractory to genetic manipulation that disrupts the coding sequence, suggesting its absence is incompatible with parasite survival in asexual blood stages. Further, the cysteine-rich domain of PfRON6 appears to be functionally important, as it cannot be truncated. The genetic data suggests that PfRON6 is a novel and potentially important component of the Plasmodium invasion machinery. Neither Pf34 nor PfRON6 were found to bind directly to human RBCs, indicating that the proteins are not directly involved in merozoite attachment to the RBC, preceding invasion. Our data highlights the possiblity that more, as yet unidentified protein complexes, in the rhoptries may be critically required for the parasites growth survival throughout the asexual life cycle stage.

Download or read book Malaria written by Institute of Medicine and published by National Academies Press. This book was released on 1991-02-01 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is making a dramatic comeback in the world. The disease is the foremost health challenge in Africa south of the Sahara, and people traveling to malarious areas are at increased risk of malaria-related sickness and death. This book examines the prospects for bringing malaria under control, with specific recommendations for U.S. policy, directions for research and program funding, and appropriate roles for federal and international agencies and the medical and public health communities. The volume reports on the current status of malaria research, prevention, and control efforts worldwide. The authors present study results and commentary on the: Nature, clinical manifestations, diagnosis, and epidemiology of malaria. Biology of the malaria parasite and its vector. Prospects for developing malaria vaccines and improved treatments. Economic, social, and behavioral factors in malaria control.

Download or read book Functional Characterization of Two Putative Telomere associated Proteins in the Malaria Parasite Plasmodium Falciparum written by Ajuh Elvis Tasih and published by . This book was released on 2013 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book RAMA Revealed written by Agnieszka Ewa Topolska and published by . This book was released on 2003 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book An Alternative Secretory Pathway in the Malaria Parasite Plasmodium falciparum written by Thuvaraka Thavayogarajah and published by GRIN Verlag. This book was released on 2017-08-31 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Doctoral Thesis / Dissertation from the year 2014 in the subject Biology - Diseases, Health, Nutrition, grade: 1.3, University of Marburg (European virtual Institute for Malaria Research), language: English, abstract: This study focuses on the discovery of an alternative secretory pathway to the ER/Golgi route in the malaria parasite P. falciparum in infected RBCs. Two proteins appeared to be promising candidates of an alternative secretory pathway: the PfADP-ribosylation factor 1 (ARF1) and the Pfadenylate kinase 2 (AK2). Both proteins contained an N-myristoylation site at their N-terminus, which is indicative for N-myristoylation. N-myristoylation is a co-translational modification of a protein, whereby a fatty acid (myristate) is irreversibly attached to the glycine residue at the N-terminus of a protein via the PfN-myristoyltransferase (NMT). A preceding proteomic analysis of the parasitophorous vacuole and a reporter construct study proposed for both PfARF1 (determined by a proteomic study) and PfAK2 (determined by a reporter construct study) PV localization although both proteins lacked a signal peptide. That’s why it was hypothesized whether or not N-myristoylation would drive protein secretion across the parasite plasma membrane (PPM). The subcellular localization of the PfARF1/GFP parasites and the PfAK2/GFP parasites, respectively, were analyzed via epifluorescence microscopy and biochemical methods. In parallel, another batch of reporter constructs were generated and analyzed, where the N-myristoylation site of PfARF1 (this study) and PfAK2 (Ma et al., 2012), respectively, was removed (PfARF1G2A/GFP and PfAK2G2A/GFP). Live cell imaging showed that the fusion protein ARF1/GFP was localized as dot-like structures in the parasite. In contrast, the phenotype of the fusion protein of the PfARF1G2A/GFP parasites showed an evenly distributed signal in the parasite cytosol. Further analysis of the subcellular localization of the PfARF1 strongly supports its localization to compartments of the early secretory pathway of the parasite, but no localization in the PV. In contrast, the fusion protein PfAK2/GFP localized to a ring-like structure around the parasite indicating PV localization. The PfAK2G2A/GFP parasites showed a cytosolic localization of the fusion protein (Ma et al., 2012). Biochemical analyses revaled that the fusion protein PfAK2/GFP was secreted into the PV when the N-myristoylation site was present. Furthermore, it could be shown that the N-terminus of the PfAK2 protein is sufficient for parasite plasma membrane targeting, stable membrane anchoring and subsequent protein translocation across the PPM.

Download or read book Identification and Characterisation of HAEM Interacting Proteins from the Malaria Parasite Plasmodium Falciparum written by Naomi Vittoria Campanale and published by . This book was released on 2007 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of a Puf Protein Family Member in Malaria Parasite Plasmodium Falciparum written by Xiaoying Liang and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of gene expression is important to cellular growth and differentiation of eukaryotes, as it plays key roles in determining the eventual expression levels and cellular localizations of a large number of proteins. RNA-binding proteins (RBPs) with conserved RNA-binding domains (RBDs) are key molecules in controlling gene expression. These conserved RBDs can specifically interact with target RNAs through specific sequences and structures to regulate mRNA stability, translation efficiency and subcellular localization. One of the well-studied RBPs is the Puf family RBPs. Members of the Puf family share a highly conserved Puf RBD, which typically consists of eight imperfect tandem repeats of ~36 amino acids (aa). Here I report the studies of two Puf members, PfPuf3 in Plasmodium falciparum and its ortholog PyPuf3 in Plasmodium yoelii. Both genes could not be disrupted, suggesting that they may be essential for the intraerythrocytic developmental cycle (IDC). A time-course study of both PfPuf3 mRNA and protein indicated that PfPuf3 was expressed during the entire IDC, with peak expression in early trophozoites. Cellular fractionation demonstrated that PfPuf3 preferentially partitioned to the nuclear than the cytoplasmic fractions. Interestingly, tagging of the endogenous of PfPuf3 and PyPuf3 with the green fluorescent protein showed that both proteins were limited to the nucleus, which is in contrast to the cytoplasmic localization of PfPuf1 and PfPuf2. Further, we found PfPuf3 co-localized with a well-known nucleolus maker PfNop1, demonstrating that PfPuf3 is a nucleolar protein. Using a parasite line with PfPuf3-tagged with PTP (ProtC-TEV-ProtA), we were able to affinity purify the PfPuf3 protein complex and the associated RNAs. Proteomic analysis of the PfPuf3 complex identified 32 proteins that are associated with the 60S ribosome subunit, suggesting that PfPuf3 might be involved in ribosomal biogenesis. RNA sequencing analysis of the purified PfPuf3 complex revealed significant enrichment of the 28S rRNA and ITS2 (internal transcribed spacer 2), implying that PfPuf3 may recognize and bind to these rRNA sequences. Taken together, these results demonstrated nucleolar localization of PfPuf3 and suggested an essential function of PfPuf3 in ribosomal biogenesis.

Download or read book Identification and Characterization of Invasion related Proteins of the Malaria Parasite Plasmodium Falciparum Welch 1892 written by Louisa Wilcke and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of Genes Coding for a 235kDa Rhoptry Protein of the Malarial Parasite Plasmodium Yoelii written by Katharine Anita Sinha and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Apicoplast Proteins from the Malaria Parasite Plasmodium Falciparum written by Vanessa Levine and published by . This book was released on 2013 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of Trafficking Signals Shared by Different Types of Exported Proteins in the Human Malaria Parasite Plasmodium Falciparum written by Ann-Katrin Ullrich and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Associations Between Responses to the Rhoptry Associated Membrane Antigen of Plasmodium Falciparum and Immunity to Malaria Infection written by and published by . This book was released on 2004 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rhoptry proteins participate in the invasion of red blood cells by merozoites during the malaria parasite's asexual-stage cycle. Interference with the rhoptry protein function has been shown to prevent invasion, and three rhoptry proteins have been suggested as potential components of a vaccine against malaria. Rhoptry associated membrane antigen (RAMA) is a 170-kDa protein of Plasmodium falciparum which is processed to a 60-kDa mature form in the rhoptries. p60/RAMA is discharged from rhoptries of free merozoites and binds to the red-cell membrane before being internalized to form part of the parasitophorous vacuole of the newly developing ring. We examined the range of anti-RAMA responses in individuals living in an area of endemicity for malaria and determined its association with clinical immunity. RAMA is immunogenic during infections, and at least three epitopes within RAMA are recognized by hyperimmune sera in immunoblots. Sera from individuals living in a region of Vietnam where malaria is endemic possessed strong antibody responses toward two C-terminal regions of RAMA. Cytophilic antibody isotypes (immunoglobulin G1 [IgG1] and IgG3) predominated in humoral responses to both C-terminal epitopes. Acute episodes of P. falciparum infection result in significant boosting of levels of antibody to an epitope at the extreme C terminus of RAMA that harbors the red-cell-binding domain. Immunity to P. falciparum infection was linked to elevated levels of IgG3 responses to this functional domain of RAMA, suggesting that the region may contain a protective epitope useful for inclusion in a multiepitope vaccine against malaria.

Download or read book Identification and Characterization of Two Polypeptides Associated with the Human Malarial Parasite Plasmodium Falciparum written by Melanie Gray Edwards Small and published by . This book was released on 1988 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the RIFIN Protein Family of the Malaria Parasite Plasmodium Falciparum Welch 1897 written by Michaela Petter and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Two Plasmodium Falciparum Proteins MAHRP1 and MAHRP2 Involved in Host Cell Refurbishment written by Esther Miriam Pachlatko and published by . This book was released on 2010 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: