Download or read book Characterisation of Novel Nuclear Proteins in Plasmodium Falciparum written by Andreas Pulfer and published by . This book was released on 2010 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterisation of Three Novel Nuclear Proteins in Plasmodium Falciparum written by Stefan Schneitter and published by . This book was released on 2012 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterisation of a Novel Plasmodium Falciparum Protein written by and published by . This book was released on 2007 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Novel Nuclear Proteins in Plasmodoum Falciparum written by Oliver Dietz and published by . This book was released on 2009 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of Novel Epigenetic Pathways in the Malaria Parasite Plasmodium Falciparum written by Shamista Archana Selvarajah and published by . This book was released on 2014 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Recombinant Espression and Immunological Characterisation of a Novel Protein orf 5 of the Malaria Parasite Plasmodium Falciparum written by Niels Pfeiffer and published by . This book was released on 2002 with total page 73 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Novel Surface Proteins of Plasmodium Falciparum and Their Assessment as Molecular Targets for a Malaria Subunit Vaccine written by Anita M. Dreyer and published by . This book was released on 2012 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterisation of a Moved Plasmodium Falciparum Protein written by Louise Hinds and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of the Plasmodium Falciparum Export Complex written by Brendan Elsworth and published by . This book was released on 2015 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmodium parasites extensively remodel the mammalian host cells they infect, namely the erythrocytes and hepatocytes. This is achieved through exporting hundreds of parasite proteins into the host where they play many virulence related roles including altering membrane permeability to acquire nutrients for rapid growth and for increasing immune evasion. Proteins are secreted from the parasite into the vacuole that surrounds them, where they must then pass across the parasitophorous vacuole membrane (PVM) to gain access to the host cytoplasm. It has previously been shown that a protein complex, the Plasmodium Translocon of EXported proteins (PTEX), is found on the PVM and is most likely the protein translocon responsible for this export process. To validate and further dissect PTEX function, a conditional expression system that utilizes the glmS riboswitch was employed to conditionally knockdown the levels of PTEX150 within parasites. Using this approach, even a relatively low level of PTEX150 knockdown lead to a significant decrease in the ability of the parasites to export proteins across the PVM. This failure of protein export across the PVM arrested growth and eventually caused parasite death. Interestingly, the export of all classes of protein cargoes tested, including PfEMP1, were blocked upon PTEX150 knockdown suggesting PTEX may serve as a single portal for export. This demonstrates the importance of PTEX to the malaria parasite and validates it as a potent drug target, since blocking it would prevent hundreds of exported proteins from reaching their functional destinations. To further investigate the specific function of PTEX150 we performed C-terminal truncations in order to generate a parasite line with a partially defective protein. These lines showed destabilisation of the PTEX complex, suggesting that the C-terminus of PTEX150, while not essential for PTEX function or the absolute binding of the other components, is required for stabilising the PTEX complex. Interestingly, these parasites did not show a defect in either their ability to grow or export proteins. To further investigate the effect of PTEX150 knockdown on parasite health the new permeability pathways (NPPs) were investigated. To do this a novel method was developed to investigate NPP function using luciferase. This method is highly sensitive, reducing the amount of parasites required as well as removing the need to purify parasites from culture. The effect of PTEX150 knockdown on NPPs was unclear using this method. Due to the high sensitivity and ease of this method it was developed as a HTS to discover NPP inhibitors. Using this method the malaria box of 400 compounds was screened. Importantly, two compounds, MMV020439and MMV007571, showed inhibitory activity significantly higher than the control compound NPPB.

Download or read book Vesicle Targeting in Plasmodium Falciparum written by Lawrence Sumanjah Ayong and published by . This book was released on 2009 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins of the SNARE (Soluble N-ethylmaleimide sensitive factor attachment protein receptor) super-family have been characterized as playing an essential role in vesicle targeting and fusion in all eukaryotes. The intracellular malaria parasite Plasmodium falciparum exhibits an unusual endomembrane system that is characterized by an unstacked Golgi apparatus, a developmentally induced apical complex, and various organellar structures of parasite origin in the infected host cells. How malaria parasites target nuclear-encoded proteins to these novel compartments is a central question in Plasmodium cell biology. Ultrastructural studies elsewhere have implicated the participation of specialized vesicular elements in transport of virulence proteins, including various cytoadherance and host cell remodeling factors, into the infected erythrocyte cytoplasm. However, little is known about the machineries that define the directionality of vesicle trafficking in malaria parasites. We hypothesized that the P. falciparum SNARE proteins would exhibit novel features required for vesicle targeting to the parasite-specific compartments. We then identified for the first time and confirmed the expression of eighteen SNARE genes in P. falciparum. Members of the PfSNAREs exhibit atypical structural features (Ayong et al., 2007, Molecular & Biochemical Parasitology, 152(2), 113-122). Among the atypical PfSNAREs, PfSec22 contains an unusual insertion of the Plasmodium export element (PEXEL) within its profilin-like longin domain, preceded by an N-terminal hydrophobic segment.

Download or read book Characterisation of Two Novel Plasmodium Falciparum Rhoptry Neck Proteins written by Nicholas Proellocks and published by . This book was released on 2009 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: PfRON6 and Pf34 are expressed late in the life RBC life cycle consistent with a potential role in the invasion process. Indirect immunofluoresence assays have shown that Pf34 and PfRON6 are both found in the merozoite apex region and specifically localise to the rhoptry neck region. This localisation was confirmed by immunoelectron microscopy for PfRON6. To date Pf34 is the only GPI-anchored protein found in the rhoptry neck, and is also associated with parasite-derived detergent-resistant microdomains (DRMs). Our transfection experiments indicate that the gene that encodes PfRON6 is refractory to genetic manipulation that disrupts the coding sequence, suggesting its absence is incompatible with parasite survival in asexual blood stages. Further, the cysteine-rich domain of PfRON6 appears to be functionally important, as it cannot be truncated. The genetic data suggests that PfRON6 is a novel and potentially important component of the Plasmodium invasion machinery. Neither Pf34 nor PfRON6 were found to bind directly to human RBCs, indicating that the proteins are not directly involved in merozoite attachment to the RBC, preceding invasion. Our data highlights the possiblity that more, as yet unidentified protein complexes, in the rhoptries may be critically required for the parasites growth survival throughout the asexual life cycle stage.

Download or read book Molecular Characterisation of a Novel Cyclase from Plasmodium Falciparum written by Adam Austin Witney and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of Two Rhoptry Proteins from the Malaria Parasite Plasmodium Falciparum written by Lev M. Kats and published by . This book was released on 2008 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Phosphoproteins from the Malaria Parasite Plasmodium Falciparum written by Bernd W. Sütterlin and published by . This book was released on 1992 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book RAMA Revealed written by Agnieszka Ewa Topolska and published by . This book was released on 2003 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the RIFIN Protein Family of the Malaria Parasite Plasmodium Falciparum Welch 1897 written by Michaela Petter and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterisation of Merozoite Surface Protein 5 of Plasmodium Falciparum written by Tieqiao Wu and published by . This book was released on 2002 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: