Download or read book Cellulase Production in Batch and Continuous Culture by Trichoderma Reesei on Xylose Based Media written by Donald William Schaffner and published by . This book was released on 1989 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulase Production by Trichoderma Reesei Rut C30 written by Chi-Ming Lo and published by . This book was released on 2008 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the price of oil continues to increase and the concern over global warming heightens, finding alternative renewable sources of energy becomes more and more imperative. Considering the abundance of lignocellulosic biomass, the potential significance of its conversion to fuel such as ethanol has long been recognized. Ethanol production from lignocellulosic biomass requires the breakdown of the solid material to simple sugars that can be consumed by microorganisms. The breakdown of lignocelluloses includes the important step of hydrolyzing cellulose, which are [Beta]-1, 4 linked polymers of glucose, by a group of enzymes collectively termed cellulase. The cost of cellulase production profoundly influences the economics of the entire ethanol production process. Trichoderma reesei Rut C-30 is the most commonly used fungal strain for industrial cellulase production. With an overall goal of decreasing the cellulase production cost, this thesis work was focused on two topics: (1) investigation of the performance of different cellulase-inducing substrates in T. reesei Rut C-30 fermentation and (2) development of a more advanced model to describe the culture behaviors of T. reesei Rut C-30 grown on lactose-based media. The cellulase-inducing substrates investigated in this work included the acid-treated hydrolysate, lactose, and the sophorolipids produced by the yeast Candida bombicola. In addition to the above focused topics, the effects of culture conditions on cellulase production were also investigated. The acid-treated hardwood hydrolysates used in the study were prepared by a two-stage process, with different durations of boiling and acid concentrations. The results indicated that the inducing ability of the hydrolysates decreased with increasing boiling time (less oligomers). The observation was attributed to the lower amounts of inducing oligomers remaining after the longer boiling in acid. When compared with cultures growing on mixed carbon substrates of cellulose and glucose, the culture growing on hydrolysates showed a longer lag phase of about 24 hours (before the active cell growth began), but produced adequately comparable final cellulase activity. The study with lactose as an inducing substrate was conducted in both batch and continuous culture systems with lactose and other relevant substrates (glycerol, glucose and galactose) as the carbon source. Instead of direct ingestion, lactose is believed to be hydrolyzed by extracellular enzymes to glucose and galactose, which are then taken up by the cells. The study results indicated that glucose strongly represses the galactose metabolism: Cells started to consume galactose only after the glucose had been depleted. A mathematical model incorporating all important metabolic activities was developed to describe the culture behaviors. All of the experimental results obtained were used in model fitting to generate a set of best-fit model parameters. The study provided significant conceptual and quantitative insights to the lactose metabolism and cellulase production by T. reesei Rut C-30. This study was also the first to hypothesize and demonstrate the use of sophorolipids as the inducing substrate for cellulase production. A unique process for cellulase production using a mixed culture of T. reesei Rut C30 and Candida Bombicola growing on glycerol-based media was investigated. Hypothetically, the sophorolipids produced by C. bombicola were hydrolyzed to form sophorose, which then served as the inducer for cellulase production by T. reesei. Further study to optimize the sophorolipid-induced cellulase production process is recommended.
Download or read book Current Developments in Biotechnology and Bioengineering written by Ashok Pandey and published by Elsevier. This book was released on 2016-09-17 with total page 888 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for isolating and purifying them from a production medium. During the last few years, the tools of molecular biology and genetic and metabolic engineering have rendered tremendous improvements in the production of industrial products by fermentation. Structured by industrial product classifications, this book provides an overview of the current practice, status, and future potential for the production of these agents, along with reviews of the industrial scenario relating to their production. - Provides information on industrial bioprocesses for the production of microbial products by fermentation - Includes separation and purification processes of fermentation products - Presents economic and feasibility assessments of the various processes and their scaling up - Links biotechnology and bioengineering for industrial process development
Download or read book Journal of Scientific and Industrial Research written by and published by . This book was released on 2008-07 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulase Production by Immobilized Trichoderma Reesei in Continuous Culture written by Eugene M. Frein and published by . This book was released on 1991 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Environmental Pollutants and their Bioremediation Approaches written by Ram Naresh Bharagava and published by CRC Press. This book was released on 2017-07-06 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compilation of detailed and latest knowledge on the various types of environmental pollutants released from various natural as well as anthropogenic sources, their toxicological effects in environments, humans, animals and plants as well as various bioremediation approaches for their safe disposal into the environments. In this book, an extensive focus has been made on the various types of environmental pollutants discharged from various sources, their toxicological effects in environments, humans, animals and plants as well as their biodegradation and bioremediation approaches for environmental cleanup.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1989 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Production of Carbohydrases by Fungus Trichoderma Reesei Grown on Soy based Media written by Anthony M. Coffman and published by . This book was released on 2013 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: Trichoderma reesei RUT-C30 was cultivated in shaker flasks and pH-controlled, agitated batch fermentations to study the effects of soy-based media on the production of cellulase, xylanase, and pectinase (polygalacturonase) for the purposes of soybean polysaccharide hydrolysis. Growth on defatted soybean flour as sole nitrogen source was compared to the standard combination of ammonium sulfate, proteose peptone, and urea. Carbon source effect was also examined for a variety of substrates, including lactose, microcrystalline cellulose (Avicel), citrus pectin, soy molasses, soy flour hydrolysate, and soybean hulls (both pretreated and natural). Flask study results indicated exceptional enzyme induction by Avicel and soybean hulls, while citrus pectin, soy molasses, and soy flour hydrolysate did not promote enzyme production. Batch fermentation experiments reflected the flask system results, showing the highest cellulase and xylanase activities for systems grown with Avicel and soybean hulls at near-neutral pH levels, and the highest polygalacturonase activity resulting from growth on lactose and soybean hulls at lower pH levels, 4.0 to 4.5. Soy polysaccharides were hydrolyzed with fermentation enzyme broths of varying enzyme composition and concentration. Enzyme mixtures with larger proportions of cellulase and polygalacturonase showed slightly higher sugar release. Hydrolysis was incomplete in all experiments, solubilizing roughly one-third of the polysaccharides to oligomeric products. Additionally, methods for estimation of cell concentration via cell DNA extraction and cell wall chitin hydrolysis and a modified intracellular protein analysis procedure were investigated, showing high levels of error and interference by soy protein from the growth media. Finally, a consistent and reliable assay for polygalacturonase activity was developed and verified.
Download or read book Microbiology Abstracts written by and published by . This book was released on 1994 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Advances in Applied Microbiology Biotechnology written by and published by . This book was released on 1993-07 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Trichoderma Reesei Cellulases written by C. P. Kubicek and published by . This book was released on 1990 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proceedings of a symposium on Trichoderma cellulases held at the Technical University of Vienna September 14-16, 1989, attended by 100 scientists representing both academic institutions and industrial research laboratories.
Download or read book Current Opinion in Biotechnology written by and published by . This book was released on 1995 with total page 982 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biotechnology Research Abstracts written by and published by . This book was released on 1991 with total page 966 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monthly. Classified listing of references to worldwide articles dealing with all aspects of biotechnology. Also includes books and conferences. Each entry gives bibliographic information, institutional address of author(s), and abstract. Author and subject index.
Download or read book Biological Agricultural Index written by and published by . This book was released on 1991 with total page 2570 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Engineering Index Annual written by and published by . This book was released on 1992 with total page 2264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its creation in 1884, Engineering Index has covered virtually every major engineering innovation from around the world. It serves as the historical record of virtually every major engineering innovation of the 20th century. Recent content is a vital resource for current awareness, new production information, technological forecasting and competitive intelligence. The world?s most comprehensive interdisciplinary engineering database, Engineering Index contains over 10.7 million records. Each year, over 500,000 new abstracts are added from over 5,000 scholarly journals, trade magazines, and conference proceedings. Coverage spans over 175 engineering disciplines from over 80 countries. Updated weekly.
Download or read book Bioconversion of Waste Materials to Industrial Products written by A.M. Martin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: By covering both the general principles of bioconversion and the specific characteristics of the main groups of waste materials amenable to bioconversion methods, this new book provides the chemical, biochemical, agrochemical and process engineer with clear guidance on the use of these methods in devising a solution to the problem of industrial waste products.
Download or read book Solar Energy Update written by and published by . This book was released on 1984 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt:
