Download or read book Cellulase Production from Lignocellulosic Materials by Trichoderma Reesei written by Parminder Singh Chahal and published by . This book was released on 1987 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cellulase Production by Trichoderma Reesei Rut C30 written by Chi-Ming Lo and published by . This book was released on 2008 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the price of oil continues to increase and the concern over global warming heightens, finding alternative renewable sources of energy becomes more and more imperative. Considering the abundance of lignocellulosic biomass, the potential significance of its conversion to fuel such as ethanol has long been recognized. Ethanol production from lignocellulosic biomass requires the breakdown of the solid material to simple sugars that can be consumed by microorganisms. The breakdown of lignocelluloses includes the important step of hydrolyzing cellulose, which are [Beta]-1, 4 linked polymers of glucose, by a group of enzymes collectively termed cellulase. The cost of cellulase production profoundly influences the economics of the entire ethanol production process. Trichoderma reesei Rut C-30 is the most commonly used fungal strain for industrial cellulase production. With an overall goal of decreasing the cellulase production cost, this thesis work was focused on two topics: (1) investigation of the performance of different cellulase-inducing substrates in T. reesei Rut C-30 fermentation and (2) development of a more advanced model to describe the culture behaviors of T. reesei Rut C-30 grown on lactose-based media. The cellulase-inducing substrates investigated in this work included the acid-treated hydrolysate, lactose, and the sophorolipids produced by the yeast Candida bombicola. In addition to the above focused topics, the effects of culture conditions on cellulase production were also investigated. The acid-treated hardwood hydrolysates used in the study were prepared by a two-stage process, with different durations of boiling and acid concentrations. The results indicated that the inducing ability of the hydrolysates decreased with increasing boiling time (less oligomers). The observation was attributed to the lower amounts of inducing oligomers remaining after the longer boiling in acid. When compared with cultures growing on mixed carbon substrates of cellulose and glucose, the culture growing on hydrolysates showed a longer lag phase of about 24 hours (before the active cell growth began), but produced adequately comparable final cellulase activity. The study with lactose as an inducing substrate was conducted in both batch and continuous culture systems with lactose and other relevant substrates (glycerol, glucose and galactose) as the carbon source. Instead of direct ingestion, lactose is believed to be hydrolyzed by extracellular enzymes to glucose and galactose, which are then taken up by the cells. The study results indicated that glucose strongly represses the galactose metabolism: Cells started to consume galactose only after the glucose had been depleted. A mathematical model incorporating all important metabolic activities was developed to describe the culture behaviors. All of the experimental results obtained were used in model fitting to generate a set of best-fit model parameters. The study provided significant conceptual and quantitative insights to the lactose metabolism and cellulase production by T. reesei Rut C-30. This study was also the first to hypothesize and demonstrate the use of sophorolipids as the inducing substrate for cellulase production. A unique process for cellulase production using a mixed culture of T. reesei Rut C30 and Candida Bombicola growing on glycerol-based media was investigated. Hypothetically, the sophorolipids produced by C. bombicola were hydrolyzed to form sophorose, which then served as the inducer for cellulase production by T. reesei. Further study to optimize the sophorolipid-induced cellulase production process is recommended.

Download or read book Cellulase written by Marguerite Stevenson and published by Nova Science Publishers. This book was released on 2016-09 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes such as cellulases have been applied as tools for research and development and in almost every industrial sector including agriculture, food, beverages and animal feed, pulp and paper, textile. Cellulases is a general term that encompasses multiple enzymes that work in synergism for cellulose degradation. This book examines the production, applications and health benefits of cellulase. Chapter One describes the isolation, screening, diversity and cellulase production of microorganisms including their applications. Chapter Two discusses the factors affecting production of cellulase enzyme by microorganisms, the potential of indigenous microorganism for the bio-augmentation process of biosolids and occurrence of catabolite repression phenomenon of cellulase production during the enzymatic degradation of biosolids. Chapter Three gives an overview on cellulase microorganism producers, cellulolytic components and mechanism of action, with a focus on fungal enzymes of Trichoderma reesei, since they are predominant on the commercial cocktails used for 2G ethanol production. Chapter Four addresses substrate factors related to enzymatic hydrolysis of cellulose and lignocellulosic materials.

Download or read book Fungi and Lignocellulosic Biomass written by Christian P. Kubicek and published by John Wiley & Sons. This book was released on 2012-09-25 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harnessing fungi’s enzymatic ability to break down lignocellulolytic biomass to produce ethanol more efficiently and cost-effectively has become a significant research and industrial interest. Fungi and Lignocellulosic Biomass provides readers with a broad range of information on the uses and untapped potential of fungi in the production of bio-based fuels. With information on the molecular biological and genomic aspects of fungal degradation of plant cell walls to the industrial production and application of key fungal enzymes, chapters in the book cover topics such as enzymology of cellulose, hemicelluloses, and lignin degradation. Edited by a leading researcher in the field, Fungi and Lignocellulosic Biomass will be a valuable tool in advancing the development and production of biofuels and a comprehensive resource for fungal biologists, enzymologists, protein chemists, biofuels chemical engineers, and other research and industry professionals in the field of biomass research.

Download or read book Sustainable Degradation of Lignocellulosic Biomass written by Anuj Chandel and published by BoD – Books on Demand. This book was released on 2013-05-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.

Download or read book Recent Progress in Bioconversion of Lignocellulosics written by G.T. Tsao and published by Springer. This book was released on 2003-06-30 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes recent advances in the bioconversion of lignocellulosics. It starts with two articles on genetics and properties of cellulases and their re- tion kinetics and mechanisms. The cost of cellulases has been a hindrance to large scale use of enzymatic hydrolysis. Two articles on cellulase production by submerged fermentation and by solid state fementation are included to describe the state of the art in this area. Dilute acid hydrolysis of cellulose continues to be of interest as well as potentially useful. The most recent advances in this area is also covered. A great deal of progress has been made in genetic engineering for improved regulation of xylose fermentation by yeasts. An article on genetically engineered Saccharomyces for simulteaneous fermentation of glucose and xylose describes the importance advances made in production of fuel ethanol from lignocellulosic biomass. In recent years, there has been increasing interests in recycling and the reuse of scrap paper as well as environment considerations. A contribution is presented which describes the research perspectives in that area. Finally, recent advances in the use of lignocellulosic biomass for the p- duction of ethanol and organic acids are presented in two articles. Renewable resources are inevitably of great importance in the years to come. There is a never-ending search for better living conditions for human beings. The more resource materials can be recycled, the richer we will be.

Download or read book Fungal Cellulolytic Enzymes written by Xu Fang and published by Springer. This book was released on 2018-08-31 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides an overview of the current knowledge on cellulolytic enzymes and their applications. It summarizes the mechanisms of synthesis and hydrolysis of cellulolytic enzymes, industrial fungal strains, genetic engineering of fungal strains and application of cellulolytic enzymes. This book will be a useful reference for researchers and bioengineering experts engaged in lignocelluloses biodegradation, biomass utilization, enzyme production and fungal molecular biology.

Download or read book Hydrolysis of Lignocellulosic Waste Paper Materials by Cellulase Enzyme Components of Trichoderma Reesei Penicillium Funicolosum and Aspergillus Niger written by Mothatheo Alfred Mogale and published by . This book was released on 2004 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Lignocellulose Conversion written by Vincenza Faraco and published by Springer Science & Business Media. This book was released on 2013-06-12 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol has been recognized as a potential alternative to petroleum-derived transportation fuels. Even if cellulosic biomass is less expensive than corn and sugarcane, the higher costs for its conversion make the near-term price of cellulosic ethanol higher than that of corn ethanol and even more than that of sugarcane ethanol. Conventional process for bioethanol production from lignocellulose includes a chemical/physical pre-treatment of lignocellulose for lignin removal, mostly based on auto hydrolysis and acid hydrolysis, followed by saccharification of the free accessible cellulose portions of the biomass. The highest yields of fermentable sugars from cellulose portion are achieved by means of enzymatic hydrolysis, currently carried out using a mix of cellulases from the fungus Trichoderma reesei. Reduction of (hemi)cellulases production costs is strongly required to increase competitiveness of second generation bioethanol production. The final step is the fermentation of sugars obtained from saccharification, typically performed by the yeast Saccharomyces cerevisiae. The current process is optimized for 6-carbon sugars fermentation, since most of yeasts cannot ferment 5-carbon sugars. Thus, research is aimed at exploring new engineered yeasts abilities to co-ferment 5- and 6-carbon sugars. Among the main routes to advance cellulosic ethanol, consolidate bio-processing, namely direct conversion of biomass into ethanol by a genetically modified microbes, holds tremendous potential to reduce ethanol production costs. Finally, the use of all the components of lignocellulose to produce a large spectra of biobased products is another challenge for further improving competitiveness of second generation bioethanol production, developing a biorefinery.

Download or read book Hydrolysis of Lignocellulose Using Enzymes from Pelletized Trichoderma Reesei Fermentation written by Ying Liu and published by . This book was released on 2010 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Lignocellulose Biodegradation written by American Chemical Society. Meeting and published by . This book was released on 2004 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lignocellulose Biodegradation will be useful for chemists, biochemists, microbiologists, molecular biologists, and biochemical engineers. This book describes advances in lignocellulose biodegradation and application in biotechnology. It contains a combination of original research and review chapters. An overview chapter on lignocellulose biodegradation and applications in biotechnology focuses on recent research progress in the field. Lignocellulose Biodegradation includes sections on pretreatment, biodegradation, enzyme characterization and application.

Download or read book Current Status and Future Scope of Microbial Cellulases written by Deepak K. Tuli and published by Elsevier. This book was released on 2021-04-01 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Status and Future Scope of Microbial Cellulases not only explores the present and future of cellulase production, it also compares solid state fermentation (SSF) and submerged fermentation (SMF) for cellulase production. Chapters explore bioprocess engineering, metabolic engineering and genetic engineering approaches for enhanced cellulase production, including the application of cellulase for biofuel production. This important resource presents current technical status and the future direction of advances in cellulase production, including application of cellulases in different sectors. - Covers the present industrial scenarios and future prospect of cellulase production - Describes the molecular structure of cellulase - Explores genetic engineering, metabolic engineering and other approaches for improved cellulase production - Includes different applications of cellulases, including their application in the bioenergy sector

Download or read book Production of Trichoderma Reesei Cellulase and Enzymatic Hydrolysis of Lignocellululosic Materials for Chemical Feedstock Production written by and published by . This book was released on 1984 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cellulase written by Maurya Devendra Prasad and published by LAP Lambert Academic Publishing. This book was released on 2014-01 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial cellulases find applications in various industries and forms a major group of the industrial enzymes. Keeping all the advantages associated with microbial enymes, in this study Cellulase production have been carried out using the fungal strain of Trichoderma reesei by using three different lignocellulosic biomass under solid state fermentation (SSF)conditions. The effect of different basic fermentation parameters on enzyme production were studied. Cellulase obtained, find applications in biofuels productions and bioremediations.

Download or read book Saccharification and Fermentation of Lignocellulosic Biomass Using Trichoderma Reesei Cellulases and Saccharomyces Cerevisiae written by Yun-Chin Chung and published by . This book was released on 1996 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: The efficiency of cellulose hydrolysis under straight saccharification and simultaneous saccharification and fermentation (SSF) conditions was evaluated using three lignocellulosic materials (switchgrass, cornstover, and poplar), which had been pretreated with dilute sulfuric acid under conditions which optimized xylose concentrations in the prehydrolysate liquid. Yields of glucose, cellobiose and ethanol obtained from the pretreated feedstocks were measured over 168 hrs. The final theoretical conversions of cellulose from pretreated switchgrass, cornstover, and poplar in straight saccharification were 85-100% (average 94%), 84-100% (average 96%), and 75-100% (average 87%), respectively, while in SSF the conversions were 84-90% (average 87%), 91-96% (average 90%), 72%-82% (average 76%), respectively. The conversion rates of poplar in straight saccharification and SSF were significantly lower than those of switchgrass and cornstover. The effects of reaction parameters such as enzyme activity, cellulose availability, and yeast cell viability on the extent of hydrolysis in straight saccharification and SSF were also studied. Results indicate that the lower glucose or ethanol yields associated with some of the poplar were due to the recalcitrant nature of its cellulose. To compare accurately the efficiencies between straight saccharification and SSF, a direct method for determining the cellulose content of the feedstocks residues resulting from SSF experiments has been developed and evaluated. The method improves on classical cellulose assays by incorporating a yeast lysing enzyme to remove yeast glucans from the feedstocks residue prior to acid hydrolysis and subsequent quantification of cellulose derived glucose. A freeze-drying step was identified as necessary to render the SSF yeast cells susceptible to enzyme lysis. The method was applied to the analysis of the cellulose and yeast-glucan content of SSF residues from the three pretreated feedstocks. Cellulose assays employing the lysing enzyme preparation demonstrated relative errors up to 7.2% when yeast-associated glucan were not removed prior to analysis of SSF residues. Enzymatic lysis of SSF yeast cells may be viewed as a general preparatory procedure to be used prior to the subsequent chemical and physical analysis of SSF residues.

Download or read book Approaches to Enhance Industrial Production of Fungal Cellulases written by Manish Srivastava and published by Springer. This book was released on 2019-04-26 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulase is a key enzyme of industrial interest and plays a crucial role in the hydrolysis of cellulose, a prime component of plant cell walls. Cellulase covers a broad area in the global market of industrially important enzymes and it is considered as the third largest industrial enzyme globally. Additionally, cellulase contributes about 20% of the total enzyme market globally because of its massive demand in various industries such as in biofuel production, pulp, paper, textile, food, and beverages, as well as in detergent industries. Among these, the demand of cellulase may become frequently selected in the commercial production of biofuels in the future and thus will further increase demand of cellulase in the biofuel industry. Because biofuel production is still not realized in a cost-effective, practical implementation due to its high cost (the higher cost of biofuels is due to higher production costs of enzymes), there is a need to introduce these types of approaches, which will help to lower the cost of enzyme production for developing overall economic biofuel production.

Download or read book Current Status and Future Scope of Microbial Cellulases written by Deepak K. Tuli and published by Elsevier. This book was released on 2021-03-26 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Status and Future Scope of Microbial Cellulases not only explores the present and future of cellulase production, it also compares solid state fermentation (SSF) and submerged fermentation (SMF) for cellulase production. Chapters explore bioprocess engineering, metabolic engineering and genetic engineering approaches for enhanced cellulase production, including the application of cellulase for biofuel production. This important resource presents current technical status and the future direction of advances in cellulase production, including application of cellulases in different sectors. Covers the present industrial scenarios and future prospect of cellulase production Describes the molecular structure of cellulase Explores genetic engineering, metabolic engineering and other approaches for improved cellulase production Includes different applications of cellulases, including their application in the bioenergy sector