Download or read book Cellulase Production by Immobilized Trichoderma Reesei in Continuous Culture written by Eugene M. Frein and published by . This book was released on 1991 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulase Production in Batch and Continuous Culture by Trichoderma Reesei on Xylose Based Media written by Donald William Schaffner and published by . This book was released on 1989 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Approaches to Enhance Industrial Production of Fungal Cellulases written by Manish Srivastava and published by Springer. This book was released on 2019-04-26 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulase is a key enzyme of industrial interest and plays a crucial role in the hydrolysis of cellulose, a prime component of plant cell walls. Cellulase covers a broad area in the global market of industrially important enzymes and it is considered as the third largest industrial enzyme globally. Additionally, cellulase contributes about 20% of the total enzyme market globally because of its massive demand in various industries such as in biofuel production, pulp, paper, textile, food, and beverages, as well as in detergent industries. Among these, the demand of cellulase may become frequently selected in the commercial production of biofuels in the future and thus will further increase demand of cellulase in the biofuel industry. Because biofuel production is still not realized in a cost-effective, practical implementation due to its high cost (the higher cost of biofuels is due to higher production costs of enzymes), there is a need to introduce these types of approaches, which will help to lower the cost of enzyme production for developing overall economic biofuel production.
Download or read book Cellulase Production by Trichoderma Reesei Rut C30 written by Chi-Ming Lo and published by . This book was released on 2008 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the price of oil continues to increase and the concern over global warming heightens, finding alternative renewable sources of energy becomes more and more imperative. Considering the abundance of lignocellulosic biomass, the potential significance of its conversion to fuel such as ethanol has long been recognized. Ethanol production from lignocellulosic biomass requires the breakdown of the solid material to simple sugars that can be consumed by microorganisms. The breakdown of lignocelluloses includes the important step of hydrolyzing cellulose, which are [Beta]-1, 4 linked polymers of glucose, by a group of enzymes collectively termed cellulase. The cost of cellulase production profoundly influences the economics of the entire ethanol production process. Trichoderma reesei Rut C-30 is the most commonly used fungal strain for industrial cellulase production. With an overall goal of decreasing the cellulase production cost, this thesis work was focused on two topics: (1) investigation of the performance of different cellulase-inducing substrates in T. reesei Rut C-30 fermentation and (2) development of a more advanced model to describe the culture behaviors of T. reesei Rut C-30 grown on lactose-based media. The cellulase-inducing substrates investigated in this work included the acid-treated hydrolysate, lactose, and the sophorolipids produced by the yeast Candida bombicola. In addition to the above focused topics, the effects of culture conditions on cellulase production were also investigated. The acid-treated hardwood hydrolysates used in the study were prepared by a two-stage process, with different durations of boiling and acid concentrations. The results indicated that the inducing ability of the hydrolysates decreased with increasing boiling time (less oligomers). The observation was attributed to the lower amounts of inducing oligomers remaining after the longer boiling in acid. When compared with cultures growing on mixed carbon substrates of cellulose and glucose, the culture growing on hydrolysates showed a longer lag phase of about 24 hours (before the active cell growth began), but produced adequately comparable final cellulase activity. The study with lactose as an inducing substrate was conducted in both batch and continuous culture systems with lactose and other relevant substrates (glycerol, glucose and galactose) as the carbon source. Instead of direct ingestion, lactose is believed to be hydrolyzed by extracellular enzymes to glucose and galactose, which are then taken up by the cells. The study results indicated that glucose strongly represses the galactose metabolism: Cells started to consume galactose only after the glucose had been depleted. A mathematical model incorporating all important metabolic activities was developed to describe the culture behaviors. All of the experimental results obtained were used in model fitting to generate a set of best-fit model parameters. The study provided significant conceptual and quantitative insights to the lactose metabolism and cellulase production by T. reesei Rut C-30. This study was also the first to hypothesize and demonstrate the use of sophorolipids as the inducing substrate for cellulase production. A unique process for cellulase production using a mixed culture of T. reesei Rut C30 and Candida Bombicola growing on glycerol-based media was investigated. Hypothetically, the sophorolipids produced by C. bombicola were hydrolyzed to form sophorose, which then served as the inducer for cellulase production by T. reesei. Further study to optimize the sophorolipid-induced cellulase production process is recommended.
Download or read book Collection of Trichoderma Reesei Cellulase by Foaming written by Qin Zhang and published by . This book was released on 2007 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Facing the current energy crisis, people try to find alternative energy sources. Certain microbes can ferment soluble sugars to ethanol, which is a well known biofuel. Cellulosic biomass is the most abundant renewable resource. Cellulose can be broken down to soluble sugars by the cellulase enzymes produced only by some microbes. The combination of the above two processes, i.e., break-down of cellulose to sugars and conversion of sugars to ethanol, represents a major approach of solving the energy crisis through renewable biological processes. One of the bottle necks of this approach is the relatively high production cost of cellulase. In this research, the main task was to develop a separation process for cellulase using cost-effective and environment-friendly foam-based techniques. First, the foaming properties of the fermentation broth of Trichoderma reesei RUT C-30 were investigated. The experiment results showed that cellulase was not the dominant substance that caused the foaming of the fermentation broth, although the increase of cellulase concentration in the fermentation process appeared to parallel the increase in foaming tendency. Cellulase concentration was found to be only a weakly positive factor contributing to the foaming whereas the presence of cells and solid cellulose powders had negative effects on foaming. The cell wall was found to be hydrophobic according to the study conducted to observe the cells partition between hydrophobic (organic solvent: n-hexadecane) phase and hydrophilic (aqueous) phase. The finding of hydrophobic cell wall was consistent with the observation that the cells would come out with foam in the foaming study. To prevent the cell from being foamed out of the fermentor during the foaming process intended for cellulase collection, the cell immobilization in small (3mm*3mm*3mm) pieces of polyurethane (PU) foams with different porosity and pore size was also investigated. With some of the PU foam materials, the cells could be effectively immobilized and would not be foamed out during the foaming process. Nevertheless, this system did not function well in the typical stir-tank fermentor: the high shear stress present near the tip of the impeller blades appeared to strip the immobilized cells off the PU foam pellets and caused significant subsequent growth of free (not immobilized) cells in the broth. In a subsequent study of cellulase production using cellulose hydrolysate (CH) as the inducing substrate, it was observed that the addition of the hydrolysate resulted in higher enrichment ratios of cellulase concentration in the collected foamate (over the residual concentration in the spent broth). The foaming study was then expanded to include the addition of other cellulase substrates and substrate analogs, such as xylan hydrolysate (XH) and carboxylmethylcellulose (CMC). The results clearly demonstrated the addition of CH, XH and CMC would improve the enrichment and purification of cellulases by foaming. The improvements were attributed to the affinity binding between the substrates/substrate analogs and the cellulase enzymes and the increased partition of the substrate-enzyme complexes to the foam's air-liquid interfaces. To further pursue the above affinity foaming phenomenon for significantly improved collection and, potentially, purification of cellulase, a polymeric foaming agent was designed and synthesized. The polymeric surfactant has two parts, one is the hydrophobic region containing methylmethacrylate (MMA) and methacrylic acid (MAA), the other part is cellobiose, i.e., the substrate of the enzyme to bind with during foaming process. Unfortunately, the foaming ability of this synthetic surfactant was not good enough and its synthesis was complicated. As an easier first step, two glycolipid biosurfactants, sophorolipids and rhamnolipids, were investigated as the possible affinity foaming agents. Both glycolipids had a disaccharide moiety that was expected to bind selectively to the â-glucosidase in cellulase. While sophorolipids did not foam well, rhamnolipids showed great foaming ability and selectivity towards â-glucosidase. The enrichment ratio of â-glucosidase activity in the foamate reached up to 20 in the solutions prepared by cellulase powders (from Sigma). The significant benefits of such affinity foam collection and/or fractionation techniques were clearly shown."--Abstract.
Download or read book New and Future Developments in Microbial Biotechnology and Bioengineering written by Vijai G. Gupta and published by Elsevier. This book was released on 2016-11-15 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Cellulase System Properties and Applications covers the biochemistry of cellulase system, its mechanisms of action, and its industrial applications. Research has shed new light on the mechanisms of microbial cellulase production and has led to the development of technologies for production and applications of cellulose degrading enzymes. The biological aspects of processing of cellulosic biomass have become the crux of future research involving cellulases and cellulolytic microorganisms, as they are being commercially produced by several industries globally and are widely being used in food, animal feed, fermentation, agriculture, pulp and paper, and textile applications. The book discusses modern biotechnology tools, especially in the area of microbial genetics, novel enzymes, and new enzyme and the applications in various industries. As a professional reference, this new book is useful to all researchers working with microbial cellulase system, both academic institutions and industry-based research bodies, as well as to teachers, graduate, and postgraduate students with information on continuous developments in microbial cellulase system. The book provides an indispensable reference source for chemists, biochemical engineers/bioengineers, biochemists, biotechnologists and researchers who want to know about the unique properties of this microbe and explore its future applications. - Compiles the latest developments made and currently undergoing in the area of microbial cellulase system - Chapters are contributed from top researchers on this area around the globe - Includes information related to almost all areas of microbial cellulase system - Extensive cover of current industrial applications and discusses potential future applications
Download or read book Current Status and Future Scope of Microbial Cellulases written by Deepak K. Tuli and published by Elsevier. This book was released on 2021-04-01 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Status and Future Scope of Microbial Cellulases not only explores the present and future of cellulase production, it also compares solid state fermentation (SSF) and submerged fermentation (SMF) for cellulase production. Chapters explore bioprocess engineering, metabolic engineering and genetic engineering approaches for enhanced cellulase production, including the application of cellulase for biofuel production. This important resource presents current technical status and the future direction of advances in cellulase production, including application of cellulases in different sectors. - Covers the present industrial scenarios and future prospect of cellulase production - Describes the molecular structure of cellulase - Explores genetic engineering, metabolic engineering and other approaches for improved cellulase production - Includes different applications of cellulases, including their application in the bioenergy sector
Download or read book Trichoderma Reesei Cellulases written by C. P. Kubicek and published by . This book was released on 1990 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proceedings of a symposium on Trichoderma cellulases held at the Technical University of Vienna September 14-16, 1989, attended by 100 scientists representing both academic institutions and industrial research laboratories.
Download or read book Scientific Bulletin written by and published by . This book was released on 1985 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Annual Reports on Fermentation Processes written by George T. Tsao and published by Elsevier. This book was released on 2014-12-01 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annual Reports on Fermentation Processes, Volume 6 focuses on the significant developments in fermentation processes. This book discusses the application of immobilized living microbial cells, aerobic high-rate process for concentrated wastes, and large-scale cell culture technology. The gas-liquid mass transfer fermentation step, aeration devices, relative motion of cells and medium, and enzymes of industrial interest are also elaborated. This text likewise covers the microbial sensors for waste waters control, ventures in yeast utilization, and advances in D-xylose conversion by yeasts. Other topics include the effect of high energy radiation on lignocellulose, interaction between the solid substrate and the enzyme, and control and operation of fed-batch processes. This volume is valuable to students and researchers aiming to acquire knowledge of fermentation research and developments.
Download or read book Solar Energy Update written by and published by . This book was released on 1984 with total page 884 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulases in the Biofuel Industry written by Pratima Bajpai and published by Elsevier. This book was released on 2022-10-08 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulases in the Biofuel Industry discusses how the properties of cellulases affects the quality of the biofuels produced. Heralded as the solution to humanity’s energy problem and the savior of the world’s climate, extensive research is being carried out on biofuels but there are still gaps in our understanding. This book presents cost-effective and current scenarios for cellulase production in the biofuel industry, including the most recent advancements for obtaining cellulases with higher activity on pre-treated biomass substrates by screening and sequencing new organisms, engineering cellulases with improved properties, and by identifying proteins that can stimulate cellulases. The mechanism and efficiency of the cellulase enzyme system on cellulose is discussed with the specific classification of each cellulase enzyme, as well as explanations of the limitation of cellulases in terms of their production processes, efficiency and practical applications to biofuels. Various approaches to improve the production and efficiency of the cellulase enzyme system are evaluated, along with the current limitations that are hampering cost-effective production of cellulase and guidance on how these limitations might be resolved. Includes different approaches to improve the production and efficiency of the cellulase enzyme system Discusses the current limitations hampering the cost-effective production of cellulases Provides case studies that include essential information for those looking to adapt cellulases technology
Download or read book Annual Reports on Fermentation Processes written by George T. Tsao and published by Elsevier. This book was released on 2014-12-01 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annual Reports on Fermentation Processes, Volume 7 deliberates the significant developments in fermentation processes. This book discusses the production and applications of Trichoderma reesei cellulase, microbial utilization of gaseous alkanes, and growth of mycelium and mushroom. The immobilized cells in sensing devices, economic aspects of fermentation processes, and impact of biotechnology on the health care industry are also elaborated. This text likewise covers the industrial mammalian cell culture, microbial biomass from renewables, and by-products from lignocellulosic materials. Other topics include the MB production by mixed cultures, costs of fermentation processes, and fermentations classified by carbon substrate. This volume is a good reference for students and researchers interested in fermentation research and developments.
Download or read book ONR Far East Scientific Bulletin written by and published by . This book was released on with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulase written by Marguerite Stevenson and published by Nova Science Publishers. This book was released on 2016-09 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes such as cellulases have been applied as tools for research and development and in almost every industrial sector including agriculture, food, beverages and animal feed, pulp and paper, textile. Cellulases is a general term that encompasses multiple enzymes that work in synergism for cellulose degradation. This book examines the production, applications and health benefits of cellulase. Chapter One describes the isolation, screening, diversity and cellulase production of microorganisms including their applications. Chapter Two discusses the factors affecting production of cellulase enzyme by microorganisms, the potential of indigenous microorganism for the bio-augmentation process of biosolids and occurrence of catabolite repression phenomenon of cellulase production during the enzymatic degradation of biosolids. Chapter Three gives an overview on cellulase microorganism producers, cellulolytic components and mechanism of action, with a focus on fungal enzymes of Trichoderma reesei, since they are predominant on the commercial cocktails used for 2G ethanol production. Chapter Four addresses substrate factors related to enzymatic hydrolysis of cellulose and lignocellulosic materials.
Download or read book Energy Research Abstracts written by and published by . This book was released on 1990 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Production of Cellulase by Freely Suspended and Immobilised Trichoderma Reesei written by M. Turker and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
