Download or read book Cellular Imaging Techniques for Neuroscience and Beyond written by Floris G. Wouterlood and published by Academic Press. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Single and multiphoton confocal microscopy, and 4-pi confocal microscopy Obtaining nanoresolution via photoactivation localization microscopy (PALM) Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy Study of morphology and function via high-resolution fluorescence procedures Additional high-resolution microscopic techniques
Download or read book Cellular Imaging Techniques for Neuroscience and Beyond written by Floris G. Wouterlood and published by Academic Press. This book was released on 2012-08-08 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).
Download or read book Imaging in Neuroscience and Development written by Rafael Yuste and published by CSHL Press. This book was released on 2005 with total page 813 pages. Available in PDF, EPUB and Kindle. Book excerpt: As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Download or read book Imaging Neurons written by Rafael Yuste and published by . This book was released on 2000 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.
Download or read book In Vivo Optical Imaging of Brain Function written by Ron D. Frostig and published by CRC Press. This book was released on 2009-05-06 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques a
Download or read book Brain Imaging written by Najam Arfeen Sharif and published by . This book was released on 1989 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Guide to Research Techniques in Neuroscience written by Matt Carter and published by Academic Press. This book was released on 2022-03-26 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
Download or read book Visualizing Chemistry written by National Research Council and published by National Academies Press. This book was released on 2006-06-01 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Download or read book Discovering the Brain written by National Academy of Sciences and published by National Academies Press. This book was released on 1992-01-01 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: The brain ... There is no other part of the human anatomy that is so intriguing. How does it develop and function and why does it sometimes, tragically, degenerate? The answers are complex. In Discovering the Brain, science writer Sandra Ackerman cuts through the complexity to bring this vital topic to the public. The 1990s were declared the "Decade of the Brain" by former President Bush, and the neuroscience community responded with a host of new investigations and conferences. Discovering the Brain is based on the Institute of Medicine conference, Decade of the Brain: Frontiers in Neuroscience and Brain Research. Discovering the Brain is a "field guide" to the brainâ€"an easy-to-read discussion of the brain's physical structure and where functions such as language and music appreciation lie. Ackerman examines: How electrical and chemical signals are conveyed in the brain. The mechanisms by which we see, hear, think, and pay attentionâ€"and how a "gut feeling" actually originates in the brain. Learning and memory retention, including parallels to computer memory and what they might tell us about our own mental capacity. Development of the brain throughout the life span, with a look at the aging brain. Ackerman provides an enlightening chapter on the connection between the brain's physical condition and various mental disorders and notes what progress can realistically be made toward the prevention and treatment of stroke and other ailments. Finally, she explores the potential for major advances during the "Decade of the Brain," with a look at medical imaging techniquesâ€"what various technologies can and cannot tell usâ€"and how the public and private sectors can contribute to continued advances in neuroscience. This highly readable volume will provide the public and policymakersâ€"and many scientists as wellâ€"with a helpful guide to understanding the many discoveries that are sure to be announced throughout the "Decade of the Brain."
Download or read book Electrochemical Methods for Neuroscience written by Adrian C. Michael and published by CRC Press. This book was released on 2006-12-13 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical techniques. Today, these methods provide neurochemical information that is not accessible by other means. The growing recognition of the versatility of electrochemi
Download or read book Neurohistology and Imaging Techniques written by Radek Pelc and published by Humana. This book was released on 2020-06-25 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.
Download or read book Translational Pain Research written by Lawrence Kruger and published by CRC Press. This book was released on 2009-11-24 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the Most Rapidly Advancing Fields in Modern Neuroscience The success of molecular biology and the new tools derived from molecular genetics have revolutionized pain research and its translation to therapeutic effectiveness. Bringing together recent advances in modern neuroscience regarding genetic studies in mice and humans and the practical
Download or read book Neuroimaging in Developmental Clinical Neuroscience written by Judith M. Rumsey and published by Cambridge University Press. This book was released on 2009-02-19 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroimaging offers tremendous opportunities for gaining insights into normative development and a wide array of developmental neuropsychiatric disorders. Focusing on ontogeny, this text covers basic processes involved in both healthy and atypical maturation, and also addresses the range of neuroimaging techniques most widely used for studying children. This book will enable you to understand normative structural and functional brain maturation and the mechanisms underlying basic developmental processes; become familiar with current knowledge and hypotheses concerning the neural bases of developmental neuropsychiatric disorders; and learn about neuroimaging techniques, including their unique strengths and limitations. Coverage includes normal developmental processes, atypical processing in developmental neuropsychiatric disorders, ethical issues, neuroimaging techniques and their integration with psychopharmacologic and molecular genetic research approaches, and future directions. This comprehensive volume is an essential resource for neurologists, neuropsychologists, psychiatrists, pediatricians, and radiologists concerned with normal development and developmental neuropsychiatric disorders.
Download or read book From Neurons to Neighborhoods written by National Research Council and published by National Academies Press. This book was released on 2000-11-13 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: How we raise young children is one of today's most highly personalized and sharply politicized issues, in part because each of us can claim some level of "expertise." The debate has intensified as discoveries about our development-in the womb and in the first months and years-have reached the popular media. How can we use our burgeoning knowledge to assure the well-being of all young children, for their own sake as well as for the sake of our nation? Drawing from new findings, this book presents important conclusions about nature-versus-nurture, the impact of being born into a working family, the effect of politics on programs for children, the costs and benefits of intervention, and other issues. The committee issues a series of challenges to decision makers regarding the quality of child care, issues of racial and ethnic diversity, the integration of children's cognitive and emotional development, and more. Authoritative yet accessible, From Neurons to Neighborhoods presents the evidence about "brain wiring" and how kids learn to speak, think, and regulate their behavior. It examines the effect of the climate-family, child care, community-within which the child grows.
Download or read book Mapping the Brain and Its Functions written by Institute of Medicine and published by National Academies Press. This book was released on 1991-02-01 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Significant advances in brain research have been made, but investigators who face the resulting explosion of data need new methods to integrate the pieces of the "brain puzzle." Based on the expertise of more than 100 neuroscientists and computer specialists, this new volume examines how computer technology can meet that need. Featuring outstanding color photography, the book presents an overview of the complexity of brain research, which covers the spectrum from human behavior to genetic mechanisms. Advances in vision, substance abuse, pain, and schizophrenia are highlighted. The committee explores the potential benefits of computer graphics, database systems, and communications networks in neuroscience and reviews the available technology. Recommendations center on a proposed Brain Mapping Initiative, with an agenda for implementation and a look at issues such as privacy and accessibility.
Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Download or read book Multimodal Brain Tumor Segmentation and Beyond written by Bjoern Menze and published by Frontiers Media SA. This book was released on 2021-08-10 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
