Download or read book Cell Tissue and Organ Cultures in Neurobiology written by S. Fedoroff and published by Elsevier. This book was released on 2012-12-02 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.
Download or read book Cell Tissue and Organ Cultures in Neurobiology written by Leif Hertz and published by . This book was released on 1977 with total page 693 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tissue Culture in Neurobiology written by Ezio Giacobini and published by . This book was released on 1980 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Culture in the Neurosciences written by Jane Bottenstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
Download or read book Tissue Culture of the Nervous System written by Gordon Sato and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad vantages of these cultures.
Download or read book Culturing Nerve Cells written by Gary Banker and published by MIT Press. This book was released on 1998 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Download or read book Neuroscience Approached Thru Cell Culture written by Steven E. Pfeiffer and published by Springer. This book was released on 1982-08-30 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protocols for Neural Cell Culture written by Laurie C. Doering and published by Humana Press. This book was released on 2009-10-29 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.
Download or read book Neuroscience Approached Through Cell Culture written by Steven E. Pfeiffer and published by . This book was released on 1982 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Neurobiology written by Robert Lahue and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in knowledge have led to an increasing interest in neuro biology over the last several years. These advances have been made possible, at least in part, by the use of increasingly sophisticated methodology. Furthermore, research in the most rapidly advancing areas is essentially multidisciplinary and is characterized by contributions from many investi gators employing a variety of techniques. While a grasp of fundamental neurobiological concepts is an obvious prerequisite for those who wish to follow or participate in this field, critical awareness and evaluation of neurobiological research also requires an understanding of sophisticated methodologies. The objective of Methods in Neurobiology is the development of such critical abilities. The reader is exposed to the basic concepts, principles, and instrumentation of key methodologies, and the application of each meth odology is placed in the special context of neurobiological research. The reader will gain familiarity with the terminology and procedures of each method and the ability to evaluate results in light of the particular features of neurobiological preparations and applications.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Neuron in Tissue Culture written by L. W. Haynes and published by . This book was released on 1999-02-22 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the Handbook Series contains 73 contributions from experts in the range of fields to which neuronal tissue culture may be applied. Twenty-two keynote chapters contain methods, well-illustrated with data from the contributing authors? laboratones, which exemplify ?landmark? applications of the technique. Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum. The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book. The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons. It is essential reading for all those with research interests in neuroscience, including developmental biologists, neurologists, nerve cell physiologists and neuropharmaoologists.
Download or read book Neuronal Cell Culture written by Shohreh Amini and published by Humana. This book was released on 2022-06-09 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Download or read book Neurophysiologic Studies in Tissue Culture written by Stanley M. Crain and published by . This book was released on 1976 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Dissection and Tissue Culture Manual of the Nervous System written by Abraham Shahar and published by Wiley-Liss. This book was released on 1989-12-05 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: At last … a collection of practical protocols for explanting and manipulating neuronal and glial cells. A Dissection and Tissue Culture Manual of the Nervous System Abraham Shahar, Jean de Vellis, Antonia Vernadakis, and Bernard Haber, Editors Among research laboratories involved with neuronal and glial cell cultures and their applications, there is a growing demand for a hand-book describing dissection procedures, culture preparation techniques, and the in vitro manipulation of neural cells and tissues for specific analytical purposes. A Dissection and Tissue Culture Manual of the Nervous System offers a diverse collection of methods that have been developed by and are used routinely within specialized neurobiological laboratories. Written in an easy-to-follow style, the procedures described in this unique guide are designed by experts to be applied by those with limited experience in the field. Organized into ten comprehensive sections, ninety concise contributions from leading laboratories worldwide put forth practical, stepwise protocols for neural cell manipulation and experimentation. Methods encompass: an illustrated outline of techniques for the dissection of brain areas in the fetus and the neonate the dissection of selected specialized structures, such as the ciliary ganglion organotypic. explant culture of nervous tissue dissociated culture of astrocytes, oligodendrocyte, neurons, and Schwann cells reaggregation culture of dissociated cells. Sections devoted to various tissue processing methods and experimental applications of cultured material present histochemical, autoradio-graphic, and immunocytochemical staining and visualization techniques. In situ hybridization methods, as well as preparative procedures for electron microscopy and biochemical and physiological assays, are discussed with an emphasis on methods tailored for the neurobiologist. Alternative techniques for the cultivation of the same organ or cell type from diverse animal species are juxtaposed with a varied selection of methodology and instrumentation, and complemented by key literature citations for further reading, to enable the investigator to chose the appropriate approach for a specific neurobiological application. Presented in a comb-bound format for convenient use on the laboratory bench, A Dissection and Tissue Culture Manual of the Nearvous System will be an essential research companion to graduate students, post-doctoral fellows and other laborabory investigators in cell and developmental neurobiology, neuroanatomy, neurophysiology, neuropharmacology, and biochemistry.
Download or read book Indwelling Neural Implants written by William M. Reichert and published by CRC Press. This book was released on 2007-12-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite enormous advances made in the development of external effector prosthetics over the last quarter century, significant questions remain, especially those concerning signal degradation that occurs with chronically implanted neuroelectrodes. Offering contributions from pioneering researchers in neuroprosthetics and tissue repair, Indwel
Download or read book Stem Cells and the Future of Regenerative Medicine written by Institute of Medicine and published by National Academies Press. This book was released on 2002-01-25 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.
