Download or read book Cell Surface Proteins of Gram positive Pathogenic Bacteria written by Magnus Hook and published by Frontiers Media SA. This book was released on 2021-07-14 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Bacterial Cell Surface written by S.M. Hammond and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a common statement that because of its simplicity the bacterial cell makes an ideal model for the study of a wide variety of biological systems and phenomena. While no-one would dispute that much of our under standing of biological function derives from the study of the humble bacterium, the concept of a simple life-form would be hotly disputed by any scientist engaged in the determination of the relationship between structure and function within the bacterial cell. Bacteria are particularly amenable to intensive study; their physiology can be probed with powerful biochemical, genetical and immunological techniques. Each piece of information obtained inevitably raises as many questions as answers, and can lead to a highly confused picture being presented to the lay reader. Nowhere is this more evident than in the study of the surface layers of the bacterial cell. Examination of the early electron micrographs suggested that the bacterial cytoplasm was surrounded by some sort of semi-rigid layer, possessing sufficient intrinsic strength to protect the organism from osmotic lysis. The belief that the surface layers were rather passive led to their neglect, while researchers concentrated on the superficially more exciting cytoplasmic components. Over the last twenty years our view of the bacterial envelope has undergone extensive revision, revealing a structure of enormous complexity.
Download or read book Gram positive Pathogens written by Vincent A. Fischetti and published by . This book was released on 2000 with total page 760 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the only single volume to deal with all aspects of gram–positive pathogens. It addresses the mechanisms of gram–positive bacterial pathogenicity, including the current knowledge on gram–positive structure and mechanisms of antibiotic resistance. Emphasizing streptococci, staphylococci, listeria, and spore–forming pathogens, Gram–Positive Pathogens includes chapters written by many of the leading researchers in these areas. The chapters systematically dissect these organisms biologically, genetically, and immunologically in an attempt to understand the strategies used by these bacteria to cause human disease.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Pathogenesis written by and published by Academic Press. This book was released on 1998-07-01 with total page 643 pages. Available in PDF, EPUB and Kindle. Book excerpt: Established almost 30 years ago, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research. Focuses on the methods most useful for the microbiologist interested in the way in which bacteria cause disease Includes section devoted to 'Approaches to characterising pathogenic mechanisms' by Stanley Falkow Covers safety aspects, detection, identification and speciation Includes techniques for the study of host interactions and reactions in animals and plants Describes biochemical and molecular genetic approaches Essential methods for gene expression and analysis Covers strategies and problems for disease control
Download or read book Bacterial Cell Wall written by J.-M. Ghuysen and published by Elsevier. This book was released on 1994-02-09 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.
Download or read book Protein and Sugar Export and Assembly in Gram positive Bacteria written by Fabio Bagnoli and published by Springer. This book was released on 2017-05-02 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the envelope of Gram-positive bacteria including its composition, the latest discoveries in the mechanisms behind its assembly, and its role in pathogenesis. Furthermore, new applications in biotechnology and vaccine development involving these bacteria are discussed in detail. This concise volume consists of eleven chapters by prominent experts in the field, which review the latest findings and current state of knowledge on a range of diverse yet interlinked aspects. This book is written for all researchers, clinicians and technicians engaged in basic or applied science projects on Gram-positive bacteria.
Download or read book Structural and Biochemical Investigation of Gram Positive Bacterial Surface Display Machinery written by Brendan Rayhan Amer and published by . This book was released on 2017 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many species of pathogenic Gram-positive bacteria have become resistant to commonly used antibiotics, creating a pressing need for the development of novel antimicrobial therapies. These pathogenic bacteria are surrounded by a complex peptidoglycan cell wall that is decorated with a range of macromolecules such as surface proteins, protein-based oligomeric pili, and surface glycopolymers. These structures enable the microbe to effectively interact with their environment, and in pathogenetic bacteria are frequently virulence factors that are involved in: immune system modulation, bacterial adhesion, nutrient acquisition, and spore formation. Two distinct enzyme superfamilies are primarily responsible for covalently attaching macromolecules to the Gram-positive cell wall: sortase enzymes that attach proteins and assemble pili, and LytR-Psr-CpsA (LCP) enzymes that attach glycopolymers. This dissertation presents my efforts to discover a sortase inhibitor, structural and mechanistic investigations of both sortase and LCP enzymes, and the development of a new sortase mediated ligation technology. I describe our efforts to find inhibitors against the S. aureus sortase transpeptidase. We utilized both experimental and computational methods to discover potent inhibitor scaffolds. This led to structure-activity studies that not only improved inhibitor potency, but also yielded molecules that can inhibit bacterial protein display in vivo, and appear to be non-toxic to human cells. Sortase enzymes have also proven to be valuable biotechnological conjugation tools. I describe the development of a novel sortase-mediated ligation tool. We demonstrate a 15-fold rate enhancement for existing sortase mediated ligation strategies. Additionally, I discuss studies on sortase pilus assembly and display. To gain insight into the molecular mechanism of sortase pilus assembly, we reconstituted an archetypal Gram-positive pilus assembly process in vitro. This has allowed us to generate molecular models of various intermediates of pilus assembly and define the molecular determinants of this assembly process. We have identified novel features on both the sortase and pilin precursor proteins that are essential for pilus covalent isopeptide assembly. In the concluding chapter of this dissertation, structural work characterizing the unique LCP enzyme that mediates surface protein glycosylation is presented. Work on this project reveals that LCP enzymes use a conserved phosphotransferase mechanism to attach glycopolymers to surface displayed proteins, and this may be a mechanism for actinobacteria wall teichoic acid display, a key macromolecule in the bacterial cell envelope. Combined, this work serves to further define the mechanism of Gram-positive bacterial macromolecular surface display, improved sortase-mediated bioconjugation strategies, and steps towards novel anti-infective sortase inhibitors.
Download or read book Gram Positive Pathogens written by Vincent A. Fischetti and published by John Wiley & Sons. This book was released on 2019-10-01 with total page 2803 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gram-positive bacteria, lacking an outer membrane and related secretory systems and having a thick peptidoglycan, have developed novel approaches to pathogenesis by acquiring (among others) a unique family of surface proteins, toxins, enzymes, and prophages. For the new edition, the editors have enhanced this fully researched compendium of Gram-positive bacterial pathogens by including new data generated using genomic sequencing as well as the latest knowledge on Gram-positive structure and mechanisms of antibiotic resistance and theories on the mechanisms of Gram-positive bacterial pathogenicity. This edition emphasizes streptococci, staphylococci, listeria, and spore-forming pathogens, with chapters written by many of the leading researchers in these areas. The chapters systematically dissect these organisms biologically, genetically, and immunologically, in an attempt to understand the strategies used by these bacteria to cause human disease. “This textbook comprises a superb collection of scientific knowledge making it a must-read for any graduate student, medical doctor, or investigator studying these gram-positive bacteria and inspiring future imaginations of biological knowledge.” - William R. Jacobs, Jr., PhD, Professor Microbiology & Immunology, Albert Einstein College of Medicine
Download or read book Functional Structural and Evolutionary Studies on a Family of Bacterial Surface Proteins written by Maarten de Château and published by . This book was released on 1996 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Surfaceome written by Kenneth R. Boheler and published by Humana Press. This book was released on 2017-12-20 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
Download or read book Studies on Surface Proteins of Gram positive Bacteria written by Robert Janulczyk and published by . This book was released on 2001 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Anchoring of Surface Proteins to the Cell Wall of Gram positive Bacteria written by William Wiley Navarre and published by . This book was released on 1999 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural Biology of Bacterial Pathogenesis written by Gabriel Waksman and published by Amer Society for Microbiology. This book was released on 2005 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structural Biology of Bacterial Pathogenesis explores recent developments in the understanding of the molecular basis of bacterial infectious diseases, from structures involved in adhesion and host recognition to those describing elements of bacterial secretion systems Compiles engaging, convenient reviews of current research Presents high–quality illustrations of key importance to structural biology Reviews comprehensively the molecular basis of bacterial pathogenesis Provides a link between pathology and its underlying molecular mechanisms Contributors are leading scientists in the fields of structural biology and bacterial infectious diseases
Download or read book Crystalline Bacterial Cell Surface Proteins written by Uwe B. Sleytr and published by Elsevier. This book was released on 1996-04-18 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crystalline Bacterial Cell Surface Proteins assembles information on the understanding of the occurrence, structure, chemistry, genetics, assembly, function, and application potential of S-layers. The chapters are designed to stand independent of each other and provide a complete survey of the different topics in S-layer research. This book is intended to stimulate further development in basic and applied S-layer research. Assembles present-day understanding of S-layers Provides a detailed survey of the entire field of basic and applied S-layer research Potential for broad application in biotechnology, vaccine development, diagnostics, molecular nanotechnology, and biomimetics
Download or read book Regulation of Bacterial Virulence written by Michael L. Vasil and published by American Society for Microbiology Press. This book was released on 2012-12-05 with total page 1189 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive compendium of scholarly contributions relating to bacterial virulence gene regulation. • Provides insights into global control and the switch between distinct infectious states (e.g., acute vs. chronic). • Considers key issues about the mechanisms of gene regulation relating to: surface factors, exported toxins and export mechanisms. • Reflects on how the regulation of intracellular lifestyles and the response to stress can ultimately have an impact on the outcome of an infection. • Highlights and examines some emerging regulatory mechanisms of special significance. • Serves as an ideal compendium of valuable topics for students, researchers and faculty with interests in how the mechanisms of gene regulation ultimately affect the outcome of an array of bacterial infectious diseases.
Download or read book The Mechanism of Sortase Catalyzed Cell Wall Anchoring of Surface Proteins During Gram positive Bacterial Infections written by Sarkis Krikor Mazmanian and published by . This book was released on 2002 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
