Download or read book Cell Penetrating Peptides written by Ülo Langel and published by Humana. This book was released on 2015-07-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of “naked” CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Cell-Penetrating Peptides: Methods and Protocols, Second Edition hopes to raise relevant questions for further development.
Download or read book CPP Cell Penetrating Peptides written by Ülo Langel and published by Springer. This book was released on 2019-06-03 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, a summary and update of the most important areas of CPP research are presented, whilst raising relevant questions for further development. The CPP sequences are presented and discussed throughout the book. The methods for testing CPP mechanisms are discussed in detail. Various approaches for the testing of endocytotic pathways of CPP uptake are also described. Different CPP uptake experiments are compared since it is becoming clear that it is often best to apply several methods in a complementary manner in order to most comprehensively evaluate CPP uptake mechanisms due to the complexity of these processes. A brief summary of functionality issues of CPPs, both in vitro and in vivo are discussed. Therapeutic potential of CPPs and commercial developments are discussed. The monograph is written for researchers and students in the field.
Download or read book Handbook of Cell Penetrating Peptides written by Ulo Langel and published by CRC Press. This book was released on 2006-08-15 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, the wealth of new information on the use of these peptides as transport systems has in fact served to confound the field. The constant internal change in the field of cell-penetrating peptides (CPPs) is due to recent research uncovering apparent ambiguities in cellular upta
Download or read book CPP Cell Penetrating Peptides written by Ülo Langel and published by Springer Nature. This book was released on 2023-10-18 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, a summary and update of the most important areas of cell-penetrating peptides (CPP) research are presented, while raising relevant questions for further development. The CPP sequences are presented and discussed throughout the book. The methods for testing CPP mechanisms are discussed in detail. Various approaches for the testing of endocytotic pathways of CPP uptake are also described. Different CPP uptake experiments are compared since it is becoming clear that it is often best to apply several methods in a complementary manner in order to most comprehensively evaluate CPP uptake mechanisms due to the complexity of these processes. A brief summary of functionality issues of CPPs, both in vitro and in vivo, is discussed. Therapeutic potential of CPPs and commercial developments are discussed. The present, second edition of this book is the updated and expanded version of the first edition, published in 2019. The development of the field of cell-penetrating peptides in these five years has been obvious and exciting. This second edition of the book has been partly reorganized and comprehensively expanded with the exciting research in 2019-2023. Around 2500 novel scientific articles have become available, most of them are reviewed in the second edition. Additional rapidly growing areas of high impact presented in this second edition are therapeutic developments (Chapter 16) and delivery of oligonucleotides and proteins/peptides (Chapters 5 and 6) including novel reports on genome editing with CPP assistance. Also, several additional examples are available now on clinical trials using CPPs (Chapter 15). The book is written for researchers and students in the field.
Download or read book Innovative Medicine written by Kazuwa Nakao and published by Springer. This book was released on 2015-10-13 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is devoted to innovative medicine, comprising the proceedings of the Uehara Memorial Foundation Symposium 2014. It remains extremely rare for the findings of basic research to be developed into clinical applications, and it takes a long time for the process to be achieved. The task of advancing the development of basic research into clinical reality lies with translational science, yet the field seems to struggle to find a way to move forward. To create innovative medical technology, many steps need to be taken: development and analysis of optimal animal models of human diseases, elucidation of genomic and epidemiological data, and establishment of “proof of concept”. There is also considerable demand for progress in drug research, new surgical procedures, and new clinical devices and equipment. While the original research target may be rare diseases, it is also important to apply those findings more broadly to common diseases. The book covers a wide range of topics and is organized into three complementary parts. The first part is basic research for innovative medicine, the second is translational research for innovative medicine, and the third is new technology for innovative medicine. This book helps to understand innovative medicine and to make progress in its realization.
Download or read book Cell Penetrating Peptides written by Ulo Langel and published by CRC Press. This book was released on 2002-05-29 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major problems with drug delivery is the inability of large hydrophilic compounds to pass through the lipid membrane of the cell, thus making drugs such as polypeptides and oligonucleotides of limited therapeutic value. Until recently, the transport of such molecules into the cytoplasmic and nuclear compartments of living cells seemed a
Download or read book Cell Penetrating Peptides written by Ulo Langel and published by CRC Press. This book was released on 2002-05-29 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major problems with drug delivery is the inability of large hydrophilic compounds to pass through the lipid membrane of the cell, thus making drugs such as polypeptides and oligonucleotides of limited therapeutic value. Until recently, the transport of such molecules into the cytoplasmic and nuclear compartments of living cells seemed a far off goal. Hence, the discovery that peptides derived from the HIV Tat protein and homeodomain of the Antennapedia transcription factor (penetratin) can translocate across the plasma membrane of live cells has opened up exciting new possibilities in biomedical research. This book fills the need for a comprehensive update of the research concerning these specialized peptides, called cell-penetrating peptides (CPPs), to which the drug molecule can attach and pass through the cell membrane. Divided into three parts, Cell-Penetrating Peptides: Processes and Applications covers classes of cell-penetrating peptides; mechanisms of cell penetration, interactions of cell-penetrating peptides with plasma membranes, and lipid models; and applications of cell-penetrating peptides. These divisions cover all information from basic biochemistry to clinical application, including a clear description of the general rules for cellular uptake of the transport peptides. The overview of work in this area will lead to better designed novel transport peptides to achieve the more successful application of CPPs in research and therapy.
Download or read book Synthetic DNA Delivery Systems written by Dan Luo and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.
Download or read book Peptides and Peptide based Biomaterials and their Biomedical Applications written by Anwar Sunna and published by Springer. This book was released on 2017-10-26 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid-binding peptides have been used increasingly as molecular building blocks in nanobiotechnology as they can direct the assembly and functionalisation of a diverse range of materials and have the ability to regulate the synthesis of nanoparticles and complex nanostructures. Nanostructured materials such as β-sheet fibril-forming peptides and α-helical coiled coil systems have displayed many useful properties including stimulus-responsiveness, modularity and multi-functionality, providing potential technological applications in tissue engineering, antimicrobials, drug delivery and nanoscale electronics. The current situation with respect to self-assembling peptides and bioactive matrices for regenerative medicine are reviewed, as well as peptide-target modeling and an examination of future prospects for peptides in these areas.
Download or read book Handbook of In Vivo Chemistry in Mice written by Katsunori Tanaka and published by John Wiley & Sons. This book was released on 2020-04-27 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides timely, comprehensive coverage of in vivo chemical reactions within live animals This handbook summarizes the interdisciplinary expertise of both chemists and biologists performing in vivo chemical reactions within live animals. By comparing and contrasting currently available chemical and biological techniques, it serves not just as a collection of the pioneering work done in animal-based studies, but also as a technical guide to help readers decide which tools are suitable and best for their experimental needs. The Handbook of In Vivo Chemistry in Mice: From Lab to Living System introduces readers to general information about live animal experiments and detection methods commonly used for these animal models. It focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release. Topics include: currently available mouse models; biocompatible fluorophores; radionuclides for radiodiagnosis/radiotherapy; live animal imaging techniques such as positron emission tomography (PET) imaging; magnetic resonance imaging (MRI); ultrasound imaging; hybrid imaging; biocompatible chemical reactions; ligand-directed nucleophilic substitution chemistry; biorthogonal prodrug release strategies; and various selective targeting strategies for live animals. -Completely covers current techniques of in vivo chemistry performed in live animals -Describes general information about commonly used live animal experiments and detection methods -Focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release -Places emphasis on material properties required for the development of appropriate compounds to be used for imaging and therapeutic purposes in preclinical applications Handbook of In Vivo Chemistry in Mice: From Lab to Living System will be of great interest to pharmaceutical chemists, life scientists, and organic chemists. It will also appeal to those working in the pharmaceutical and biotechnology industries.
Download or read book Practical Medicinal Chemistry with Macrocycles written by Eric Marsault and published by John Wiley & Sons. This book was released on 2017-09-12 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: Including case studies of macrocyclic marketed drugs and macrocycles in drug development, this book helps medicinal chemists deal with the synthetic and conceptual challenges of macrocycles in drug discovery efforts. Provides needed background to build a program in macrocycle drug discovery –design criteria, macrocycle profiles, applications, and limitations Features chapters contributed from leading international figures involved in macrocyclic drug discovery efforts Covers design criteria, typical profile of current macrocycles, applications, and limitations
Download or read book Cell Penetrating Peptides written by Makoto Oba and published by John Wiley & Sons. This book was released on 2022-11-30 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell-Penetrating Peptides The definitive reference on the rational design of cell-penetrating peptides enables readers to develop tailor-made peptides for their specific needs. In recent years, cell-penetrating peptides (CPPs) have become valuable tools for the cellular delivery of proteins, nucleic acids, and drugs. These small peptide sequences can be artificially designed and synthesized with custom-made characteristics to mediate the efficient and non-toxic transport of biomolecules, drugs, or nanoparticles into the cell. Cell-Penetrating Peptides: Design, Development, and Applications provides an up-to-date account of the development and use of CPPs for delivering membrane-impermeable bioactive molecules into cells. Bringing together contributions from leading researchers from around the world, this comprehensive volume describes the characteristics and mechanisms of CPPs as well as their application in both medicine, biotechnology and agriculture. Covers rational design and development of cell-penetrating peptides for use in cellular delivery of small molecule drugs, proteins, nucleic acids, and nanoparticles Presents the chemical and biological characteristics of CPP action in vitro and in vivo Describes the structure and design principles of both synthetic and naturally occurring CPPs Discusses key medical applications of CPPs such as oral delivery, intranasal delivery, and clinical trials Cell-Penetrating Peptides: Design, Development, and Applications is an essential resource for biochemists, medicinal chemists, molecular biologists, biotechnologists, and researchers studying CPPs in both academia and industry.
Download or read book Cell Penetrating Peptides written by Ülo Langel and published by Humana. This book was released on 2021-11-12 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of “naked” CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.
Download or read book Antimicrobial Peptides written by Katsumi Matsuzaki and published by Springer. This book was released on 2019-04-12 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of antimicrobial peptides (AMPs), their mechanisms of antimicrobial action, other activities, and various problems that must still be overcome regarding their clinical application. Divided into four major parts, the book begins with a general overview of AMPs (Part I), and subsequently discusses the various mechanisms of antimicrobial action and methods for researching them (Part 2). It then addresses a range of activities other than antimicrobial action, such as cell penetration, antisepsis, anticancer, and immunomodulatory activities (Part 3), and explores the prospects of clinical application from various standpoints such as the selective toxicity, design, and discovery of AMPs (Part 4). A huge number of AMPs have been discovered in plants, insects, and vertebrates including humans, and constitute host defense systems against invading pathogenic microorganisms. Consequently, many attempts have been made to utilize AMPs as antibiotics. AMPs could help to solve the urgent problem of drug-resistant bacteria, and are also promising with regard to sepsis and cancer therapy. Gathering a wealth of information, this book will be a bible for all those seeking to develop antibiotics, anti-sepsis, or anticancer agents based on AMPs.
Download or read book Introduction to Peptide Science written by Ian W. Hamley and published by John Wiley & Sons. This book was released on 2020-06-08 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides an interdisciplinary introduction to peptide science, covering their properties and synthesis, as well as many contemporary applications Peptides are biomolecules comprised of amino acids which play an important role in modulating many physiological processes in our body. This book presents an interdisciplinary approach and general introduction to peptide science, covering contemporary topics including their applicability in therapeutics, peptide hormones, amyloid structures, self-assembled structures, hydrogels, and peptide conjugates including lipopeptides and polymer-peptide conjugates. In addition, it discusses basic properties and synthesis clearly and concisely. Taking a logical approach to the subject, Introduction to Peptide Science gives readers the fundamental knowledge that is required to understand the cutting-edge material which comes later in the book. It offers readers in-depth chapter coverage of the basic properties of peptides; synthesis; amyloid and peptide aggregate structures; antimicrobial peptides and cell-penetrating peptides; and peptide therapeutics and peptide hormones. Introduces readers to peptide science, including synthesis and properties Provides unique content covering properties, synthesis, self-assembly, aggregation, and applications Summarizes contemporary topics in an accessible fashion including applications in therapeutics, peptide hormones, amyloid structures, self-assembled structures, hydrogels, and peptide conjugates including lipopeptides Presented at an introductory level for the benefit of students and researchers who are new to the subject Introduction to Peptide Science is an ideal text for undergraduate students of chemistry, biochemistry, and other related biological subjects, and will be a valuable resource for postgraduate students and researchers involved in peptide science and its applications.
Download or read book Delivery Technologies for Biopharmaceuticals written by Lene Jorgensen and published by John Wiley & Sons. This book was released on 2009-10-23 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in biotechnology have provided scientists with an increasing number of biopharmaceuticals such as novel peptide and protein drugs as well as nucleic acid based drugs for gene therapy. However, successful delivery of these biopharmaceuticals is a major challenge because their molecular properties lead to poor physical and chemical stability in the body and limited membrane permeability. Therefore researchers are developing a range of new delivery technologies and materials to enable these new drugs to be delivered intact to their target sites. Delivery Technologies for Biopharmaceuticals describes strategies to overcome the main barriers for successful delivery of therapeutic peptides, proteins, and nucleic acid-based drugs or vaccines related to the site of administration and the target site. Many of the approaches described are reported in formulations in current clinical trials as well as in marketed products. Contents include: challenges in delivery of biopharmaceuticals novel formulation approaches for peptide and protein injectables non-viral chemical vectors and viral technology for delivery of nucleic acid based drugs immune response, adjuvants and delivery systems for vaccines several examples of delivery systems for different biopharmaceuticals a critical assessment of delivery technologies for biopharmaceuticals Delivery Technologies for Biopharmaceuticals is an essential single-volume introduction to the technologies used by researchers to ensure efficient delivery of this exciting new class of drugs. It will be of value to researchers and students working in drug delivery, formulation, biopharmaceuticals, medicinal chemistry, and new materials development.
Download or read book Peptide Drug Discovery and Development written by Miguel Castanho and published by John Wiley & Sons. This book was released on 2011-10-24 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: Filling a real knowledge gap, this handbook and ready reference is both modern and forward-looking in its emphasis on the "bench to bedside" translational approach to drug development. Clearly structured into three major parts, the book stakes out the boundaries of peptide drug development in the preclinical as well as clinical stages. The first part provides a general background and focuses on the characteristic strengths and weaknesses of peptide drugs. The second section contains five cases studies of peptides from diverse therapeutic fields, and the lessons to be learned from them, while the final part looks at new targets and opportunities, discussing several drug targets and diseases for which peptide drugs are currently being developed.
