Download or read book Cell Cycle Control in CHO Cells written by Esther Hulleman and published by . This book was released on 2000 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Techniques in Cell Cycle Analysis written by Joe W. Gray and published by Springer Science & Business Media. This book was released on 1987-02-04 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Cell Cycle Analysis.
Download or read book Cell Cycle Control written by Tim Humphrey and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that “all cells come from cells.” In recent years, considerable effort has been applied to the identification of the basic molecules and mechanisms that regulate the cell cycle in a number of different organisms. Such studies have led to the elucidation of the central paradigms that underpin eukaryotic cell cycle control, for which Lee Hartwell, Tim Hunt, and Paul Nurse were jointly awarded the Nobel Prize for Medicine and Physiology in 2001 in recognition of their seminal contributions to this field. The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development, and cell death. This new phase of integrated cell cycle research provides further challenges and opportunities to the biological and medical worlds in applying these basic concepts to understanding the etiology of cancer and other proliferative diseases.
Download or read book Metabolic and Bioprocess Engineering of Production Cell Lines for Recombinant Protein Production written by Betina da Silva Ribeiro and published by Logos Verlag Berlin GmbH. This book was released on 2012 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dissolved carbon dioxide has been identified as an important process parameter affecting cell growth, productivity and product quality (e.g. glycosylation) of recombinant proteins when exceeding critical levels, occurring especially in industrial large-scale cell culture processes due to the increased hydrostatic pressure. As CO2 can easily pass the cellular membrane and thereby influence intracellular pH, important cellular processes (e.g. cell cycle regulation, enzymes of TCA cycle) are directly influenced by pCO2 and dependent bicarbonate concentration. Consequently, process control strategies attend to keep pCO2 within physiological range. In a metabolic engineering approach an antibody producing CHO cell line stably expressing human carbonic anhydrase (hCAII), the enzyme that catalyzes the equilibrium of CO2 in aqueous solutions, was generated and used to characterize CO2 effects in simulated CO2 acid load and high CO2 levels as they occur in large scale mammalian cell culture. The cell line expressing active hCAII showed more rapid initial re-alkalinization of cytoplasm after induced CO2 acid load.
Download or read book Cell Cycle Synchronization written by Zhixiang Wang and published by Springer Nature. This book was released on 2022-08-31 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Download or read book Cell Biology and Biotechnology written by Melvin S. Oka and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Biology and Biotechnology: Novel Approaches to Increased Cellular Productivity contains the proceedings of the symposium by the same name held in Cambridge, Massachusetts, January 30 - February 2, 1992. State-of-the-art research is presented on: the IGF-1 Receptor and Gene Expression During the Cell Cycle; Attachment Control of Fibroplast Proliferation; Cell Biology and Serum-Free Mouse Embryo Cells; Immunoglobulin Production Stimulating Factors; Erythropoietin Control of Programmed Death in Erythroid Progenitors; Prohormone Processing Enzymes and Protein Production; Control of Translation Initiation by Phosphorylation; Protein Retention in the Endoplasmic Reticulum Mediated by GPR78; Molecular Approaches Towards Manipulating the Expression of the Glucose Related Proteins in Mammalian Cells; Protein Folding in the Endoplasmic Reticulum; Sorting of Membrane Proteins in the Endocytic and Exocytic Pathways; CIS-Acting Elements Which Regulate Immunoglobulin Gene Transcription.
Download or read book The Cell Cycle written by P. C. L. John and published by Cambridge University Press. This book was released on 1981-12-31 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Animal Cell Technology Basic Applied Aspects written by Kazumi Yagasaki and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells, but also to ascertain their abilities to be used in industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of present state-of-the-art in Japan and other countries where this field is well advanced. The Proceedings will be useful for the cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working in either academic environments or in industries of biotechnology and pharmacy.
Download or read book Progress in Cell Cycle Research written by Laurent Meijer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: The "Progress in Cell Cycle Research" series is dedicated to serve as a collection of reviews on various aspects of the cell division cycle, with special emphasis on less studied aspects. We hope this series will continue to be helpful to students, graduates and researchers interested in the cell cycle area and related fields. We hope that reading of these chapters will constitute a "point of entry" into specific aspects of this vast and fast moving field of research. As PCCR4 is being printed several other books on the cell cycle have appeared (ref. 1-3) which should complement our series. This fourth volume of PCCR starts with a review on RAS pathways and how they impinge on the cell cycle (chapter 1). In chapter 2, an overview is presented on the links between cell anchorage -cytoskeleton and cell cycle progression. A model of the Gl control in mammalian cells is provided in chapter 3. The role of histone acetylation and cell cycle contriol is described in chapter 4. Then follow a few reviews dedicated to specific cell cycle regulators: the 14-3-3 protein (chapter 5), the cdc7/Dbf4 protein kinase (chapter 6), the two products of the pI6/CDKN2A locus and their link with Rb and p53 (chapter 7), the Ph085 cyclin-dependent kinases in yeast (chapter 9), the cdc25 phophatase (chapter 10), RCCI and ran (chapter 13). The intriguing phosphorylation dependent prolyl-isomerization process and its function in cell cycle regulation are reviewed in chapter 8.
Download or read book Influence of C MYC Up regulation on Cell Cycle Kinetics and the Proteome of Cho Cells written by Darrin Kuystermans and published by . This book was released on 2009 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Progress in Cell Growth Process Research written by Takumi Hayashi and published by Nova Publishers. This book was released on 2007 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: When used in the context of reproduction of living cells the phrase "cell growth" is shorthand for the idea of "growth in cell populations by means of cell reproduction." During cell reproduction one cell (the "mother" cell) divides to produce two daughter cells. Cell proliferation, which depends on the intimately linked processes of growth and division, is a fundamental systems-level attribute of all life forms. The precise regulation of proliferation in response to internal and external cues is critical for development, tissue renewal and evolutionary fitness, while the disregulation of cell proliferation underlies a variety of human diseases, most notably cancer and ageing. Historically, breakthroughs in our understanding of cell growth and division have derived from cross-fertilisation of results and ideas from researchers studying a wide range of model organisms, from yeast to humans. The basis for cell proliferation entails the control of key signalling and cell cycle regulators through transcriptional, translational, post-translational, genetic and epigenetic mechanisms. Indeed, many conceptual breakthroughs in cell regulation have derived from analyses of basic cell cycle mechanisms. This book is dedicated to new research from around the globe in this field.
Download or read book Cell Cycle Checkpoint Control Protocols written by Howard B. Lieberman and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer.
Download or read book Cell Cycle Control written by Michele Pagano and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addressing the regulation of the eukaryotic cell cycle, this book brings together experts to cover all aspects of the field, clearly and unambiguously, delineating what is commonly accepted in the field from the problems that remain unsolved. It will thus appeal to a large audience: basic and clinical scientists involved in the study of cell growth, differentiation, senescence, apoptosis, and cancer, as well as graduates and postgraduates.
Download or read book Cell Culture and Its Application written by Ronald Acton and published by Elsevier. This book was released on 2012-12-02 with total page 771 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research. The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology. The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-virus interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control. Cell biologists and researchers will find this book invaluable.
Download or read book Reproduction of Eukaryotic Cells written by David M. Prescott and published by Elsevier. This book was released on 2012-12-02 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reproduction of Eukaryotic Cells organizes in a single source the principal facts and observations on the cell life cycle and reproduction of eukaryotic cells. The aim is to increase the overall understanding of how these cells reproduce themselves and how this reproduction is regulated. The book begins with a discussion of the sections of the cell cycle and regulation of cell reproduction. Separate chapters on cell growth, cell synchrony, the G1 period, S period, and G2 period follow. Subsequent chapters are devoted to activities during cell division; cell cycle changes in surface morphology; the role of cyclic AMP (cAMP) and cyclic GMP (cGMP) in regulation of cell reproduction; and changes in nuclear proteins, RNA synthesis, and enzyme activities during the cell cycle. The final chapter covers the genetic analysis of the cell cycle.
Download or read book Progress in Cell Cycle Research written by Laurent Meijer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its second year, Progress in Cell Cycle Research was conceived to serve as an up to date introduction to various aspects of the cell division cycle. Although an annual review in any field of scientific investigation can never be as current as desired, especially in the cell cycle field, we hope that this volume will be helpful to students, to recent graduates considering a de1liation in subject and to investigators at the fringe of the cell cycle field wishing to bridge frontiers. An instructive approach to many subjects in biology is often to make comparisons between evolutionary distant organisms. If one is willing to accept that yeast represent a model primitive eukaryote, then it is possible to make some interesting comparisons of cell cycle control mechanisms between mammals and our little unicellular cousins. By and large unicellular organisms have no need for intracellular communication. With the exception of the mating phenomenon in S. cerevisiae and perhaps some nutritional sensing mechanisms, cellular division of yeast proceeds with complete disregard for neighbourly communication. Multicellular organisms on the other hand, depend entirely on intracellular communication to maintain structural integrity. Consequently, elaborate networks have evolved to either prevent or promote appropriate cell division in multicellular organisms. Yet, as described in chapter two the rudimentary mechanisms for fine tuning the cell division cycle in higher eukaryotes are already apparent in yeast.
