Download or read book Cell Culture and Its Application written by Ronald Acton and published by Elsevier. This book was released on 2012-12-02 with total page 771 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research. The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology. The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-virus interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control. Cell biologists and researchers will find this book invaluable.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Culture of Animal Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 1993-12-29 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 897 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Download or read book Plant Cell and Tissue Culture A Tool in Biotechnology written by Karl-Hermann Neumann and published by Springer Science & Business Media. This book was released on 2009-04-28 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about.
Download or read book Plant Tissue Culture written by S.S. Bhojwani and published by Elsevier. This book was released on 2012-12-02 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.
Download or read book Plant Tissue Culture and Its Bio technological Application written by W. Barz and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Cell and Tissue Culture A Tool in Biotechnology written by Karl-Hermann Neumann and published by Springer Nature. This book was released on 2020-10-01 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors’ group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.
Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Download or read book Biomaterials Artificial Organs and Tissue Engineering written by L Hench and published by Elsevier. This book was released on 2005-09-27 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maintaining quality of life in an ageing population is one of the great challenges of the 21st Century. This book summarises how this challenge is being met by multi-disciplinary developments of specialty biomaterials, devices, artificial organs and in-vitro growth of human cells as tissue engineered constructs. Biomaterials, Artificial Organs and Tissue Engineering is intended for use as a textbook in a one semester course for upper level BS, MS and Meng students. The 25 chapters are organized in five parts: Part one provides an introduction to living and man-made materials for the non-specialist; Part two is an overview of clinical applications of various biomaterials and devices; Part three summarises the bioengineering principles, materials and designs used in artificial organs; Part four presents the concepts, cell techniques, scaffold materials and applications of tissue engineering; Part five provides an overview of the complex socio-economic factors involved in technology based healthcare, including regulatory controls, technology transfer processes and ethical issues. Comprehensive introduction to living and man-made materials Looks at clinical applications of various biomaterials and devices Bioengineering principles, materials and designs used in artificial organs are summarised
Download or read book New Insights into Cell Culture Technology written by Sivakumar Joghi Thatha Gowder and published by BoD – Books on Demand. This book was released on 2017-05-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]
Download or read book Cell Culture Technology written by Cornelia Kasper and published by Springer. This book was released on 2018-10-10 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.
Download or read book Eukaryotic Cell Cultures written by Ronald T. Acton and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second International Cell Culture Congress was structured as was the First Congress to bring together scientists from academia and industry to discuss the use of cell culture in support of bioscience. It was felt that a forum whereby state-of the-art presentations were followed by informal workshops would provide opportunity for the greatest exchange of information. Within the atmosphere of the workshop, problems common to basic as well as applied research were discussed and directions for the future were brought to light. These proceedings reflect and epitomize those discussions. Although it is difficult to cover all scientific disciplines utilizing cells in culture, we feel key areas were addressed at the Congress and are herein presented. Considerable emphasis has been given to the methods for establishing cells in culture and characterizing the cells once established as well as the improved technology for growing established cell lines. Examples of how recombinant DNA technology is being used to manipulate genes within mammalian cells, to clone mammalian genes and to insert them in prokaryotes has been included. Major emphasis has been given to the use of lymphocytes in culture for understanding immune responsiveness and the culturing of a variety of cell types as a means to understand disease states.
Download or read book Basic Cell Culture Protocols written by Cheryl D. Helgason and published by Humana Press. This book was released on 2016-08-23 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
Download or read book Human Cell Culture Protocols written by Ragai R. Mitry and published by Humana Press. This book was released on 2016-08-23 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.
Download or read book Cell Culture and Its Application the First International Cell Culture Congress Birmingham Alabama September 21 25 1975 written by Ronald T. Acton and published by . This book was released on 1977 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
