Download or read book Cardiac Extracellular Matrix written by Eric G. Schmuck and published by Springer. This book was released on 2018-09-20 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book on cardiac extracellular matrix (ECM) features three sections, Fundamental Science, Pre-Clinical and Translational Science, and Clinical Applications. In the Fundamental Science section, we will cover the spectrum of basic ECM science from ECM’s role in development, biomechanical properties, cardiac ECM influence of cardiomyocyte biology, pathophysiology of ECM in heart disease, and ECM in tissue engineering. Section two, Preclinical and Translational Science, will discuss cardiac ECM technologies in the clinical pipeline including approaches to ECM as a therapeutic, animal models of cardiac research, tracking and imaging methods of cardiac ECM, and cGMP manufacturing and regulatory considerations for ECM based therapeutics. Finally, the third section, Clinical Applications, will highlight the clinical experience around cardiac ECM including therapeutic strategies targeting scar tissue in the heart, Clinical trial design and regulatory considerations, current human clinical trials in cardiovascular medicine and the role of pharmaceutical and biotech companies in the commercialization of ECM technologies for cardiovascular indications. This book provides a comprehensive review for basic and translational researchers as well as clinical practitioners and those involved in commercialization, regulatory and entrepreneurial activities.
Download or read book Mechanisms of Heart Failure written by Pawan K. Singal and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mechanisms of Heart Failure is based on papers selected from poster presentations made at the International Conference on Heart Failure, Winnipeg, May 20-23, 1994. Although the entire book is one continuous discussion of subcellular mechanisms of heart failure and its treatment, the presentation has been divided into three sections: the opening section on the subcellular basis of heart failure includes discussions of cytokines, signal transduction, metabolism, extracellular matrix, organ level changes and newer approaches to understanding the pathogenesis of heart failure. The second section focuses on the pathophysiological aspects of cardiomyopathies and their treatment. In the final section, medical, surgical and pharmacological approaches to the treatment of heart failure are discussed in clinical and animal laboratory settings.
Download or read book Extracellular Matrix for Cardiovascular Reconstruction written by Steve Bibevski and published by Frontiers Media SA. This book was released on 2021-06-01 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topic editor Robert G. Matheny is employed by company CorMatrix Inc.. All other topic editors declare no competing interests with regards to the Research Topic subject.
Download or read book Cell Biology of Extracellular Matrix written by E.D. Hay and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the ten-year interval since the first edition of this volume went to press, our knowledge of extracellular matrix (ECM) function and structure has enor mously increased. Extracellular matrix and cell-matrix interaction are now routine topics in the meetings and annual reviews sponsored by cell biology societies. Research in molecular biology has so advanced the number of known matrix molecules and the topic of gene structure and regulation that we won dered how best to incorporate the new material. For example, we deliberated over the inclusion of chapters on molecular genetics. We decided that with judicious editing we could present the recent findings in molecular biology within the same cell biology framework that was used for the first edition, using three broad headings: what is extracellular matrix, how is it made, and what does it do for cells? Maintaining control over the review of literature on the subject of ECM was not always an easy task, but we felt it was essential to production of a highly readable volume, one compact enough to serve the the student as an introduction and the investigator as a quick update on graduate the important recent discoveries. The first edition of this volume enjoyed con hope the reader finds this edition equally useful. siderable success; we D. Hay Elizabeth vii Contents Introductory Remarks 1 Elizabeth D. Hay PART I. WHAT IS EXTRACELLULAR MATRIX? Chapter 1 Collagen T. F. Linsenmayer 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. The Collagen Molecule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2. 1. Triple-Helical Domain(s) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Download or read book Remodelling of the Cardiac Extracellular Matrix in Ageing and Heart Failure written by Margaux Ann Horn and published by . This book was released on 2010 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Decellularization Methods of Tissue and Whole Organ in Tissue Engineering written by Abdol-Mohammad Kajbafzadeh and published by Springer Nature. This book was released on 2021-09-28 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume is the first of a series that introduces safe, feasible, and practical decellularization and recellularization techniques for tissue and organ reconstruction. We have put special emphasis on the research areas most likely to develop well-engineered scaffolds for tissue and organ engineering, while presenting easily applicable bench-to-bedside approaches highlighting the latest technical innovations in the field. This book includes both a fundamental discussion for a broad understanding of the basis of tissue repair and substitution, as well as chapters written by world renowned specialists from 20 countries providing deeper discussions and analysis of related sub disciplines. Within these pages, the reader will find state-of-the-art protocols and current clinical challenges in cell and tissue biology, including accurate and comprehensive information on extracellular matrices, natural biomaterials, tissue dynamics, morphogenesis, stem cells, cellular fate progressions, cell and tissue properties for in-vitro and in-vivo applications. This comprehensive and carefully organized treatise provides a clear framework for graduate students and postdoctoral researchers new to the field, but also for researchers and practitioners looking to expand their knowledge on tissue and organ reconstruction.
Download or read book Interstitial Fibrosis in Heart Failure written by Francisco Villarreal and published by Springer Science & Business Media. This book was released on 2005 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interstitial Fibrosis in Heart Failure, edited by Francisco J. Villarreal, M.D., Ph.D., provides a timely and integrative review of the basics of cardiac extracellular matrix structure. Topics covered include how cardiac remodeling influences its disposition, abundance and function; possible non-invasive techniques for diagnosis; and potential drug-based or molecular therapeutic strategies that may interrupt or even reverse the course of the development of cardiac fibrosis. This resource for both clinicians and scientists aims to cover state-of-the-art findings relevant to cellular and molecular processes underlying cardiac fibrosis including basic elements of structure, function, diagnosis and treatment.
Download or read book Modeling the Cardiac Extracellular Matrix to Identify Age Specific Modulators of Cardiomyocyte Function written by Mara Ariel Kauss and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The extracellular matrix (ECM) is a complex network of proteins that surrounds cells in all tissues, providing structural and biochemical cues that influence a multitude of cellular functions. In the heart, the ECM provides the local support and context for highly dynamic cardiomyocytes that are continuously contracting to pump blood throughout the body. However, the cardiac phenotype changes drastically throughout fetal development and postnatal maturation. Fetal cardiomyocytes are small, proliferative, glycolytic cells with disorganized sarcomeric structures. In the adult, however, cardiomyocytes become elongated, aligned, post-mitotic cells that rely on oxidative respiration and have highly organized sarcomeres. These ontogenic changes provide a dramatic increase in contractile efficiency, but sacrifice the heart's proliferation-dependent ability to regenerate after injury. As a result, heart disease is a major cause of death worldwide, and has warranted a wealth of research efforts to model cardiac tissue function and disease. During cardiac development and maturation, the ECM also undergoes age-dependent changes that can impact various cardiac functions, but the extent of its influence is poorly understood. To model the age-specific cardiac ECM in vitro, we created cell-derived matrices (CDMs) from cardiac fibroblasts to provide the structural, biophysical and chemical context of cardiac-specific ECM. In doing so, we sought to avoid certain caveats of established CDM protocols, which enhance matrix adhesion and thickness via introduction and promotion of singular matrix proteins, skewing the matrix composition, and confounding comparisons made between CDMs. We, therefore, developed a protocol that enhances matrix adhesion and deposition, respectively, by combining an L-polydopamine coating and macromolecular crowding to produce CDMs composed of cell-produced ECM. This methodology was applied to the study of age-dependent phenotypic and functional changes observed in the heart by comparing the morphologic, metabolic and proliferative responses of cardiomyocytes to CDMs produced by fetal and adult cardiac fibroblasts. Additionally, mass spectrometry proteomics identified the enrichment of ECM proteins in fetal and adult CDMs, enabling loss-of-function studies via genetic silencing of enriched proteins during CDM production. Using this strategy, we identified collagen VI as necessary for maximal oxidative respiration in stem cell-derived cardiomyocytes, which is a key component of cardiomyocyte maturation. Furthermore, we conducted a screen of all age-specific enriched CDM proteins for their influence over DNA synthesis in cardiomyocytes, and found that laminin Îł2, plasmin-mediated signaling in fibroblasts, and fibrosis-associated proteins exerted inhibitory effects on proliferation. Overall, these results demonstrate the development and application of age-specific cardiac CDMs to dissecting the influence of ECM proteins on cardiomyocyte function.
Download or read book Extracellular Matrix written by and published by BoD – Books on Demand. This book was released on 2021-10-27 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding extracellular matrix (ECM) structure and function is important for developing biomedical applications that are as close to ‘native’ as possible. Written by pioneering scientists from all over the world, this book reports research and new developments in the field of collagen structure, function, and biomechanics and discusses the relevance of hyaluronic acid and its therapeutic uses. It gives readers a glimpse of what is current in this area and we hope it piques their interest in learning more about ECM biology.
Download or read book Cardiovascular Regenerative Medicine written by Vahid Serpooshan and published by Springer. This book was released on 2019-06-29 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive and up-to-date resource on the use of regenerative medicine for the treatment of cardiovascular disease. It provides a much-needed review of the rapid development and evolution of bio-fabrication techniques to engineer cardiovascular tissues as well as their use in clinical settings. The book incorporates recent advances in the biology, biomaterial design, and manufacturing of bioengineered cardiovascular tissue with their clinical applications to bridge the basic sciences to current and future cardiovascular treatment. The book begins with an examination of state-of-the-art cellular, biomaterial, and macromolecular technologies for the repair and regeneration of diseased heart tissue. It discusses advances in nanotechnology and bioengineering of cardiac microtissues using acoustic assembly. Subsequent chapters explore the clinical applications and translational potential of current technologies such as cardiac patch-based treatments, cell-based regenerative therapies, and injectable hydrogels. The book examines how these methodologies are used to treat a variety of cardiovascular diseases including myocardial infarction, congenital heart disease, and ischemic heart injuries. Finally, the volume concludes with a summary of the most prominent challenges and perspectives on the field of cardiovascular tissue engineering and clinical cardiovascular regenerative medicine. Cardiovascular Regenerative Medicine is an essential resource for physicians, residents, fellows, and medical students in cardiology and cardiovascular regeneration as well as clinical and basic researchers in bioengineering, nanomaterial and technology, and cardiovascular biology.
Download or read book Dilated Cardiomyopathy written by Gianfranco Sinagra and published by Springer. This book was released on 2019-05-17 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book presents a comprehensive overview of dilated cardiomyopathy, providing readers with practical guidelines for its clinical management. The first part of the book analyzes in detail the disease’s pathophysiology, its diagnostic work up as well as the prognostic stratification, and illustrates the role of genetics and gene-environment interaction. The second part presents current and future treatment options, highlighting the importance of long-term and individualized treatments and follow-up. Furthermore, it discusses open issues, such as the apparent healing phenomenon, the early prognosis of arrhythmic events or the use of genetic testing in clinical practice. Offering a multidisciplinary approach for optimizing the clinical management of DCM, this book is an invaluable aid not only for the clinical cardiologists, but for all physicians involved in the care of this challenging disease.
Download or read book Cardiac Extracellular Matrix written by Bryn Brazile and published by . This book was released on 2016 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Myocardial infarction (MI) is the leading cause of death among men and women in the United States. Once a person suffers from a MI, the heart wall will undergo a dynamic and time dependent change, as it goes through the inflammatory phase, proliferative phase, and healing phase. During these phases, the necrotic tissue is removed, and the extracellular matrix (ECM) that holds the cardiomyocytes is altered by an increase in type I collagen, which leads to a scar formation in the infarcted area. The goal of this dissertation is to better understand the role of the cardiac ECM biomechanics in heart physiology, pathophysiology (MI), and regeneration. Three Aims were hence pursued. In Aim 1, we investigated cardiac ECM architecture in intact acellular hearts using diffusion tensor-magnetic resonance imaging (DT-MRI); additionally, we characterized the biomechanical and structural properties of cardiac ECM at different anatomical locations of the left ventricle wall. In Aim 2, we characterized the biomechanical and structural properties of scar ECM during the acute to chronic stages of MI using a rat heart model, in order to better understanding the time course changes in scar ECM biomechanics/microenvironment. In Aim 3, we determined if large mammals (pig heart model) have the capability to fully regenerate a resected piece of the heart apex during the neonatal stage, in which cardiac ECM is still in a developmental phase. The hope is to apply the obtained knowledge in cardiac ECM biomechanics to improve the effectiveness and efficiency of treatments, such as stem cell injection, scar tissue repairing, and regenerative intervention.
Download or read book Cardiac Tissue Engineering written by Milica Radisic and published by Humana Press. This book was released on 2014-07-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
Download or read book Biomaterials for Cardiac Regeneration written by Erik J. Suuronen and published by Springer. This book was released on 2014-11-12 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers readers a comprehensive biomaterials-based approach to achieving clinically successful, functionally integrated vasculogenesis and myogenesis in the heart. Coverage is multidisciplinary, including the role of extracellular matrices in cardiac development, whole-heart tissue engineering, imaging the mechanisms and effects of biomaterial-based cardiac regeneration, and autologous bioengineered heart valves. Bringing current knowledge together into a single volume, this book provides a compendium to students and new researchers in the field and constitutes a platform to allow for future developments and collaborative approaches in biomaterials-based regenerative medicine, even beyond cardiac applications.
Download or read book Characterization of a Naturally Derived Cardiac Extracellular Matrix Hydrogel written by Stephen YuHsiang Lin and published by . This book was released on 2012 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: The extracellular matrix (ECM) is a complex composition of proteins, proteoglycans, and glycosaminoglycans. Biological scaffolds composed of ECM have been used to help repair a variety of tissues in the body. It has been shown that an extracellular matrix material can be generated by decellularizing cardiac tissue and processed into injectable formulations for cardiac tissue engineering. When brought to physiological conditions, the matrix material will self-assemble into a porous hydrogel. The objective of the present study is to prepare the gel form of the myocardial matrix, characterize and tailor material properties of the gel, compare material properties to a human derived myocardial matrix gel, and finally evaluate the ability of the gel to support the growth of smooth muscle cells in vitro. Material properties including viscosity, swelling ratio, gelation kinetics, and stiffness were assessed. The results of the study show that material properties of the myocardial matrix can be tailored by adjusting gelation conditions. Changes in final pH, salt concentration, and protein concentration had effects on viscosity and gelation kinetics but not swelling ratio. This study also shows that there are differences in material properties between that of the porcine myocardial matrix and the human myocardial matrix. Finally the study demonstrated that myocardial matrix supported the adhesion and proliferation of rat aortic smooth muscle cells under static conditions.
Download or read book Calcific Aortic Valve Disease written by Elena Aikawa and published by BoD – Books on Demand. This book was released on 2013-06-12 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to population aging, calcific aortic valve disease (CAVD) has become the most common heart valve disease in Western countries. No therapies exist to slow this disease progression, and surgical valve replacement is the only effective treatment. Calcific Aortic Valve Disease covers the contemporary understanding of basic valve biology and the mechanisms of CAVD, provides novel insights into the genetics, proteomics, and metabolomics of CAVD, depicts new strategies in heart valve tissue engineering and regenerative medicine, and explores current treatment approaches. As we are on the verge of understanding the mechanisms of CAVD, we hope that this book will enable readers to comprehend our current knowledge and focus on the possibility of preventing disease progression in the future.
Download or read book Myocardial Tissue Engineering written by Aldo R. Boccaccini and published by Springer Science & Business Media. This book was released on 2011-08-29 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Myocardial tissue engineering (MTE), a concept that intends to prolong patients’ life after cardiac damage by supporting or restoring heart function, is continuously improving. Common MTE strategies include an engineered ‘vehicle’, which may be a porous scaffold or a dense substrate or patch, made of either natural or synthetic polymeric materials. The function of the substrate is to aid transportation of cells into the diseased region of the heart and support their integration. This book, which contains chapters written by leading experts in MTE, gives a complete analysis of the area and presents the latest advances in the field. The chapters cover all relevant aspects of MTE strategies, including cell sources, specific TE techniques and biomaterials used. Many different cell types have been suggested for cell therapy in the framework of MTE, including autologous bone marrow-derived or cardiac progenitors, as well as embryonic or induced pluripotent stem cells, each having their particular advantages and disadvantages. The book covers a complete range of biomaterials, examining different aspects of their application in MTE, such as biocompatibility with cardiac cells, mechanical capability and compatibility with the mechanical properties of the native myocardium as well as degradation behaviour in vivo and in vitro. Although a great deal of research is being carried out in the field, this book also addresses many questions that still remain unanswered and highlights those areas in which further research efforts are required. The book will also give an insight into clinical trials and possible novel cell sources for cell therapy in MTE.
