Download or read book Cancer and Zebrafish written by David M. Langenau and published by Springer. This book was released on 2016-05-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.
Download or read book Cancer and Zebrafish written by David M. Langenau and published by Springer. This book was released on 2016-05-10 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.
Download or read book The Zebrafish in Biomedical Research written by Samuel Cartner and published by Academic Press. This book was released on 2019-11-22 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Zebrafish in Biomedical Research: Biology, Husbandry, Diseases, and Research Applications is a comprehensive work that fulfills a critical need for a thorough compilation of information on this species. The text provides significant updates for working vivarium professionals maintaining zebrafish colonies, veterinarians responsible for their care and well-being, zoologists and ethologists studying the species, and investigators using the species to gain critical insights into human physiology and disease. As the zebrafish has become an important model organism for the study of vertebrate development and disease, organ function, behavior, toxicology, cancer, and drug discovery, this book presents an important resource for future research. - Presents a complete view of the zebrafish, covering their biology, husbandry, diseases and research applications - Includes the work of world-renowned authors - Provides the first authoritative and comprehensive treatment of zebrafish in biomedical research as part of the ACLAM series
Download or read book Genetic Analysis of the Cell Cycle and Cancer in Zebrafish written by Kathleen Lindahl Pfaff and published by . This book was released on 2006 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using HeLa cells, we found that the requirement for SIL in mitotic spindle organization is conserved in mammalian cells. A polyclonal antibody revealed endogenous SIL localizes to the mitotic spindle poles during metaphase. Similar to the defects in the csp mutant zebrafish, two short hairpin RNAs targeted against SIL caused microtubule disorganization in HeLa cells. The combination of results from the zebrafish and mammalian studies show that SIL is required for proper organization of the mitotic spindle.
Download or read book Zebrafish Model for Biomedical Research written by Prasan R. Bhandari and published by Springer Nature. This book was released on 2022-05-30 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the latest information on the significance of zebrafish as an ideal model for researching the biomedical field, with references. This book also focused on the evidence of zebrafish as a model in cardiovascular, neurologic, psychiatric and metabolic research. In addition, the book also includes the research carried out on zebrafish in hepatic, renal, ophthalmic, and ENT related areas. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish models including specific research in the area of skin disorders, endocrine diseases, nutritional disorders, gastrointestinal, hematological disorders and cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and its suitability for modeling various diseases, providing a critical resource on the potential attributes of the zebrafish as a pharmacological model. In terms of scope, this book is a useful tool for young researchers, professors and pharmaceutical scientists for understanding the significance of zebrafish as an emerging pharmacological model that can significantly aid in the process of drug discovery and development.
Download or read book Zebrafish written by FableVision and published by Simon and Schuster. This book was released on 2012-06-26 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vita is a purple-haired singing queen, and she has big dreams for her rock group, Zebrafish. But when she holds auditions for the back-up band, tryouts are sorely lacking in the musical ability department. Luckily her new bandmates—Walt, Tanya, Jay, and Plinko—have other talents. But lately Tanya has been missing a lot of rehearsals. When Vita discovers why, she realizes that the group can be about much more than one person, one band, or even one school. A concert won’t cure Tanya’s cancer or make her treatments any less painful, but, in their own way, Zebrafish can make a difference—and the tale of their unlikely success is sure to strike a chord with young readers looking to make their world a better place.
Download or read book The Effects of Methylmercury on Breast Cancer Progression in Zebrafish written by Sara N. Morrow and published by . This book was released on 2016 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: Breast cancer is defined as a group of malignant cells that originate in the breast and have the potential to spread to distant sites in the body, most commonly the liver, lungs, bones and brain. Many factors increase the risk of breast cancer, including but not limited to environmental factors, age, race, gender and obesity. Methylmercury (MeHg) is an environmental metalloestrogen, a type of metal that mimics the activity of estrogen. Previous studies have shown that low concentrations of MeHg cause breast cancer proliferation via the estrogen receptor (ER), but that at high concentrations it causes cell death. However, the long-term effects of MeHg exposure on breast cancer development are unknown and thus worth researching. Many studies have used xenografts in adult zebrafish (Danio rerio) to research metastatic capability. By using xenografts of mammalian breast cancer cells in zebrafish, the effects of MeHg in an in vivo system can be studied in regards to tumor progression. It is hypothesized that dietary MeHg treatment of zebrafish transplanted with human breast cancer cells will increase tumor size and the number of tumors and will result in micrometastases. In order to test this hypothesis, zebrafish from Professor Lanni’s lab in the Biochemistry Department at Wheaton College were used. First, fish were injected with fluorescently-labeled MCF-7 breast cancer cells. Fish survival and health as well as tumor growth and metastasis were measured. Once it was known that tumors could be successfully grown, an eight-week experiment was designed in which zebrafish were injected with fluorescently-labeled MCF-7 cells. Every week, fish were imaged under a fluorescent scope; after the eight-week period, fish were sacrificed and dissected for tumors. Although further troubleshooting is necessary, a protocol was successfully developed for the injection and growth of breast tumor cells in zebrafish.
Download or read book The Zebrafish Disease Models and Chemical Screens written by and published by Academic Press. This book was released on 2011-09-09 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - This volume concentrates on Disease Models and Chemical Screens
Download or read book Zebrafish in Development and Disease written by Gokhan Dalgin and published by Frontiers Media SA. This book was released on 2019-12-06 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are only a few vertebrate systems that can be used to model human diseases for biomedical discovery. The zebrafish model provides key advantages over existing models. Their externally developing embryos provide high-throughput non-invasive imaging, chemical screening, forward and reverse genetics, and their regeneration capacity make zebrafish a valuable system for novel discovery. Developmental studies using zebrafish has influenced discoveries in many human health-related conditions. This Research Topic covers all aspects of zebrafish studies, providing developmental mechanisms to human health conditions. The aim of the Research Topic was to foster a platform to bring all levels of zebrafish research including but not limited to development, disease, regeneration, drug screening, bioinformatics and Omics studies.
Download or read book Model Systems for Infectious Disease and Cancer in Zebrafish written by European Molecular Biology Organization and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Metastasis written by Ulrike S. Stein and published by Humana. This book was released on 2022-04-03 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides the most recent developments and methodologies on metastatic process, formation, and detection. Chapters guide readers through functional metastasis in vitro assays, non-mouse and mice metastasis models, methods for imaging metastasis, analyzing the tumor microenvironment, senescence and inflammation with respect to metastasis, methods to investgate the premetastatic/ metastatic, detecting biomarkers in patient, and bioinformatics to simulate the metastatic process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metastasis: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Download or read book The Zebrafish Disease Models and Chemical Screens written by and published by Academic Press. This book was released on 2017-01-25 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Zebrafish: Disease Models and Chemical Screens
Download or read book Chemical Genetics and Cancer Models in Zebrafish written by Ryan David Murphey and published by . This book was released on 2006 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tumor Microenvironment written by Alexander Birbrair and published by Springer Nature. This book was released on 2021-10-18 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses novel concepts in cancer biology, focusing on different factors that affect the tumor microenvironment. Topics covered include sex-based differences in the tumor microenironment, dormancy in the tumor microenvironment, the influence of obesity on the tumor microenvironment, and much more. Taken alongside its companion volumes, Tumor Microenvironment: Novel Concepts covers the latest research on various aspects of the tumor microenvironment, as well as future directions. Useful for introducing the newer generation of researchers to the history of how scientists studied the tumor microenvironment as well as how this knowledge is currently applied for cancer treatments, it will be essential reading for advanced cell biology and cancer biology students, as well as researchers seeking an update on research on the tumor microenvironment.
Download or read book Cancer Biomarkers written by Gagan Deep and published by Humana. This book was released on 2023-02-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response. From a radiography method to an examination of single-cell RNA-seq and computational analysis tools in cancer research, this book delves into many techniques that could provide valuable molecular information about the tumor and its microenvironment components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Biomarkers: Methods and Protocols offers researchers multiple helpful ways to study cancer-associated molecular biomarkers.
Download or read book A GNAQ 11 driven Zebrafish Cancer Model Identifies MITF and YAP as Key Determinants for Uveal Melanoma written by Hannah R. Hagen and published by . This book was released on 2019 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uveal melanoma (UM) is a cancer of eye melanocytes that has a particularly poor prognosis and a dearth of treatment options. In contrast to cutaneous melanoma (CM), which is driven by oncogenic mutation of BRAF or NRAS, UM is driven by oncogenic mutations in GNAQ or GNA11 (>80% of patients). We developed a zebrafish model of UM by expressing GNAQ[superscript Q209L] in the melanocytes of p53-/- zebrafish. In this model, oncogenic GNAQ activates biological programs involved in pigmentation and tumorigenesis. The melanocyte lineage transcription factor MITF plays a well-defined role in CM tumorigenesis, wherein MITF expression is absolutely necessary for tumorigenesis and regulates proliferative versus invasive tumor phenotypes. In contrast, here we elucidated a novel tumor suppressor function for MITF in UM. In the context of oncogenic GNAQ, loss of mitfa accelerates tumorigenesis and reduces tumor reliance on p53 mutation. Expression of oncogenic GNAQ can also overcome mitfa-/- tumor inhibition in BRAF-driven CM. Moreover, mitfa's tumor suppressive role is generalizable to UM and mitfa-/- accelerates tumorigenesis driven by oncogenic GNA11 or CYSLTR2, a GNAQ/11 upstream receptor. Interestingly, we show that GNAQ directly regulates pigment programs, as evidenced by hyperpigmentation patches that develop even in the absence of mitfa. Furthermore, the pigmentation and tumorigenesis phenotypes are decoupled, suggesting differential regulation of these programs downstream of oncogenic GNAQ. The observation that mitfa has different roles in CM vs UM led us to investigate the different signaling mechanisms of BRAF- vs GNAQ-driven melanoma. Oncogenic GNAQ activates the downstream pathways YAP and PLC[beta], and there is conflicting evidence implicating the relative importance of these two signaling axes in driving UM tumorigenesis. Here, we outline a central role for YAP for in in vivo UM tumorigenesis. Constitutively active YAP[superscript S127A;S381A] drives rapid UM tumorigenesis, furthermore, YAP staining was ubiquitous across UM tumors. Finally, we show here for the first time that PLCB4D630Y was sufficient to drive tumors at long latency. Together, these findings clarify the genetic pathways that drive UM formation.
Download or read book Alternatives to Animal Testing written by Hajime Kojima and published by Springer. This book was released on 2018-12-27 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book presents recent advances in the pure sciences that are of significance in the quest for alternatives to the use of animals in research and describes a variety of practical applications of the three key guiding principles for the more ethical use of animals in experiments – replacement, reduction, and refinement, collectively known as the 3Rs. Important examples from across the world of implementation of the 3Rs in the testing of cosmetics, chemicals, pesticides, and biologics, including vaccines, are described, with additional information on relevant regulations. The coverage also encompasses emerging approaches to alternative tests and the 3Rs. The book is based on the most informative contributions delivered at the Asian Congress 2016 on Alternatives and Animal Use in the Life Sciences. It will be of value for those working in R&D, for graduate students, and for educators in various fields, including the pharmaceutical and cosmetic sciences, pharmacology, toxicology, and animal welfare. The free, open access distribution of Alternatives to Animal Testing is enabled by the Creative Commons Attribution license in International version 4: CC BY 4.0.
