Download or read book Calorimetric and Fluorescence Characterization of Peptide lipid Interactions written by Melanie D. Myers and published by . This book was released on 1987 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Peptide Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
Download or read book Calorimetric and Fluorescence Characterization of Peptide lipide Actions written by Melanie D. Myers and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biophysical Interactions of Peptides and Their Analogues with Lipid Membranes written by Anja Stulz and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Many drugs, displaying a wide range of structures and diverse applications, can cross or bind to lipid membranes. Quantitative understanding of membrane interactions is thus important for several therapeutic approaches. First, membrane permeabilization represents the dominating mode of action of antimicrobial peptides (AMPs) and their synthetic mimics (SMAMPs). In terms of clinical applications, selectivity for bacterial over mammalian membranes is as important as good activity. Second, membrane interactions might influence loading, retaining, and releasing drugs from lipid-based drug delivery systems in a time controlled and targeted manner. Understanding the binding behaviour of the peptide drug exenatide to lipid membranes is not only important for characterization of its release from vesicular phospholipid gels, but might also help to understand other complex peptide-lipid interactions. The main aim of this thesis was to derive a mechanistic understanding of interactions of peptides and their analogues with model lipid membranes with a focus on the lipid composition of a membrane. Membrane permeabilization induced by AMPs and SMAMPs was quantified by a lifetime-based leakage assay using calcein-loaded vesicles. Different leakage behaviours were identified by considering active concentrations, strengths of individual leakage events, L1, and cumulative kinetics. Further experiments using isothermal titration calorimetry (ITC), monolayer adsorption measurements, and differential scanning calorimetry (DSC) helped to characterize the initial binding of AMPs and SMAMPs to lipid membranes and their propensity to induce electrostatic lipid clustering. Leakage experiments showed that the leakage behaviour changes with both, the structure of the AMP or SMAMP and the lipid composition of the membrane. The activity seems to increase if a membrane-active agent favours a permeabilization mechanism to which the particular lipid composition is especially susceptible. A closer look at kinetic profiles allowed distinguishing leakage induced by asymmetric stress from leakage events that occur stochastically. Very hydrophobic and unselective compounds seem to act mainly by hydrophobically driven asymmetry stress, especially when acting on zwitterionic phosphatidylcholine (PC) membranes. This mechanism brings about poor selectivity because all lipid membranes (bacterial and mammalian) contain a hydrophobic core. Stochastic leakage events, on the other hand, probably depend more on lipid compositions. Negatively charged lipids like phosphatidylglycerol (PG) or cardiolipin (CL) triggered the initial electrostatic attraction of polycationic AMPs or SMAMPs to bacterial membranes. High amounts of phosphatidylethanolamine (PE) seem to counteract the unselective asymmetry stress mechanism. Finally, especially strong leakage events were induced in vesicles containing CL. In this way, compounds that induce only rare leakage events might still be effective. In the second part of the thesis, an ITC fit model was introduced to study complex peptide-lipid interactions based on primary binding of peptide to the lipid layer and secondary binding to pre-bound peptide. Exenatide served as an exemplary peptide that interacts electrostatically with mixed POPC/POPG liposomes and self-associates at Kd = 46 μM. A global fit of various ITC curves revealed that exenatide binds primarily to a binding site at the outer membrane leaflet composed of 2-3 negatively charged POPG and some POPC molecules. Primary binding showed high affinity with a Kd1 of 0.2-0.6 μM, while secondary binding with a Kd2 of 10-46 μM was weaker. ITC was able to quantify primary and secondary binding separately, based on different binding enthalpies. Unlike ITC, other methods such as tryptophan fluorescence and microscale thermophoresis (MST) probably represent only a summary or average of both effects. Many similar ITC data can be found in literature that possibly involve primary and secondary binding effects. Those data could benefit from a fit model as presented in this thesis
Download or read book Index to American Doctoral Dissertations written by and published by . This book was released on 1986 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biophysical Characterization of Lipid protein and Lipid lipid Interactions in Model and Reconstituted Membranes Using Static and Real time X ray Diffraction and Fluorescence Quenching Techniques written by Martin Denis Caffrey and published by . This book was released on 1982 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Lipid Interactions written by C.Reyes Mateo and published by Springer. This book was released on 2005-12-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of protein–lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.
Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1989 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Progress in Protein lipid Interactions written by A. Watts and published by Elsevier Science & Technology. This book was released on 1985 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1846 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence Characterization of Protein and Lipid Dynamics written by Timothy Robert Bilderback and published by . This book was released on 1996 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence techniques were used to study the motion of protein domains in adenylate kinase, and to characterize the formation of myelin in neuron Schwann cell co-cultures. Domain motion upon substrate binding was examined in adenylate kinase using fluorescence resonance energy transfer. The process of myelin formation was examined by observing the growth and composition of myelin using a fluorescent ceramide analogue. Crystallographic evidence suggests that there is a large hinged domain motion in adenylate kinase. To test this hypothesis, resonance energy transfer measurements of substrate binding were initiated. Two tryptophan mutants were positioned at residues 133 (Y133W) and 137 (F137W), which are in the domain that closes over the ATP binding site. Mutant F86W that is located at the AMP binding site, while mutant S41W that is in the loop that closes over AMP. Energy transfer was measured between each of these tryptophans and 5-((2(-(acetyl)-amino) ethyl) amino) naphthalene-1-sulfonic acid covalently bound to the single cysteine residue at position 77. The distance between the tryptophan of the F137W mutant adenylate kinase and the AEDANS-labeled cys-77 decreased by 12.1 A upon the binding of the bisubstrate inhibitor $rm Psp1,Psp5$-$rm bis(5spprime$-$rm adenosyl)$pentaphosphate $(APsb5A).$ These results suggest that there is significant closure of the ATP binding domain upon the binding of ATP or $APsb5A.$ Unexpectedly, exposure of the enzyme to AMP also introduced a partial closure of the ATP hinged domain. Fluorescence digital imaging microscopy was used to investigate the process of myelin formation by Schwann cells in neuronal co-cultures. The uptake of the fluorescent ceramide analogue N-(5-(5,7-dimethyl BODIPY$sp{rm TM})$-1-pentanoyl) D-erythro-sphingosine and its return to the plasma membrane as the corresponding fluorescent sphingomyelin and galactocerebroside analogues were measured. The highest rate of synthesis of fluorescent sphingomyelin and galactocerebroside analogues was observed between days three and seven after induction of myelination. Additionally, the rate of lipid transport along the internode was slow since there was a quicker increase in fluorescence intensity near the cell body of the Schwann cell than near the nodes of Ranvier.
Download or read book Protein Lipid and Membrane Traffic written by North Atlantic Treaty Organization. Scientific Affairs Division and published by IOS Press. This book was released on 2000 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text concentrates on the following specific topics: the dynamic character of lipids and proteins in biological membranes; the existence of specific domains in membranes including their visualisation; the molecular mechanisms of intracellular transport of membrane constituents and the involvement of lipid-protein interactions in these processes; protein assembly; structure and folding and transport through membranes; and the intracellular sorting and targeting of individual membrane components as well as different organelles."
Download or read book Liposomes Part B written by Nejat Duzgunes and published by Elsevier. This book was released on 2003-11-19 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes in Biochemistry Liposomes in Molecular Cell Biology Liposomes in Molecular Virology
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine Chemistry and Engineering written by Soni, Shivani and published by IGI Global. This book was released on 2014-08-31 with total page 856 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a paradigm for the future, micro-scale technology seeks to fuse revolutionary concepts in science and engineering and then translate it into reality. Nanotechnology is an interdisciplinary field that aims to connect what is seen with the naked eye and what is unseen on the molecular level. The Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering examines the strengths and future potential of micro-scale technologies in a variety of industries. Highlighting the benefits, shortcomings, and emerging perspectives in the application of nano-scale technologies, this book is a comprehensive reference source for synthetic chemists, engineers, graduate students, and researchers with an interest in the multidisciplinary applications, as well as the ongoing research in the field.
Download or read book Liposomes in Gene Delivery written by Danilo D. Lasic and published by CRC Press. This book was released on 1997-03-13 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. Liposomes in Gene Delivery covers both-molecular biologists will gain a basic knowledge of lipids, liposomes, and other gene delivery vehicles; lipid and drug delivery scientists will better understand DNA, molecular biology, and DNA manipulation. Topics include an introduction to nucleic acids, a theoretical description of DNA, recombinant technology, lipids and liposomes, stability and interaction properties of lipids and liposomes, complexation of lipids and liposomes with DNA plasmids, gene expression of genosomes in various models, structure-activity relationships, and transfection models. This is an excellent introductory text for graduate students, scientists, and researchers in molecular and cell biology, genetics, biochemistry, physical chemistry, colloid science, pharmacology, molecular science, and medicine.
Download or read book CPP Cell Penetrating Peptides written by Ülo Langel and published by Springer Nature. This book was released on 2023-10-18 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, a summary and update of the most important areas of cell-penetrating peptides (CPP) research are presented, while raising relevant questions for further development. The CPP sequences are presented and discussed throughout the book. The methods for testing CPP mechanisms are discussed in detail. Various approaches for the testing of endocytotic pathways of CPP uptake are also described. Different CPP uptake experiments are compared since it is becoming clear that it is often best to apply several methods in a complementary manner in order to most comprehensively evaluate CPP uptake mechanisms due to the complexity of these processes. A brief summary of functionality issues of CPPs, both in vitro and in vivo, is discussed. Therapeutic potential of CPPs and commercial developments are discussed. The present, second edition of this book is the updated and expanded version of the first edition, published in 2019. The development of the field of cell-penetrating peptides in these five years has been obvious and exciting. This second edition of the book has been partly reorganized and comprehensively expanded with the exciting research in 2019-2023. Around 2500 novel scientific articles have become available, most of them are reviewed in the second edition. Additional rapidly growing areas of high impact presented in this second edition are therapeutic developments (Chapter 16) and delivery of oligonucleotides and proteins/peptides (Chapters 5 and 6) including novel reports on genome editing with CPP assistance. Also, several additional examples are available now on clinical trials using CPPs (Chapter 15). The book is written for researchers and students in the field.
