Download or read book Bioremediation of Trichloroethylene by Use of Polymer Bio beads and Quantification of Diffusion of Trichloroethylene Through Various Polymer Membranes written by Kirsten E. Hillyer and published by . This book was released on 2016 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Trichloroethylene is a wide spread carcinogenic contaminate in groundwater, present in roughly 60% America's Superfund sites. Beginning in the early 1900's, TCE was commonly used as an anesthetic, food processing agent, industrial degreaser, and dry cleaning agent. In 1989 the Environmental Protection Agency (EPA) implemented a maximum contaminate level (MCL) on chlorinated solvents such as TCE. Although TCE usage is strictly regulated now, the previous 100+ years of usage has left a significant amount of contamination in the environment worldwide. TCE is a dense non-aqueous phase liquid (DNAPL), making it particularly difficult to remediate. TCE travels down through groundwater until it reaches a confining layer, resulting in a persistent contamination problem for decades. Bioremediation has moved to the forefront as cost effective treatment technology for the remediation of chlorinated aliphatic hydrocarbons (CAHs), but many of these methods are still in the research phase due to the struggles the environment presents. Elevated dissolved oxygen, low pH, and high concentration of contaminates contribute to the inability of unprotected microorganisms to effectively reduce TCE. Encapsulation of microorganisms into "bio-beads" presents an opportunity to address each of these environmental challenges. For this thesis bio-beads, were developed utilizing biodegradable polymer and a reductive dechlorinating bacterium consortia known as KB-1, were subjected to various concentrations of TCE. The TCE rate of degradation was monitored to assess the kinetics of TCE reduction and organism viability. To better understand the mass transfer of TCE through the bio-beads, the diffusion coefficient of TCE through the various polymers used to create the beads was quantified. The quantification was completed by casting the polymers in membranes. With the quantification of the diffusion coefficient of TCE through various polymer membranes, development of the bio-beads can be modified in the future to improve the reaction kinetics of the bioremediation of TCE.

Download or read book Diffusion Mechanisms Measurements and Models with Applications in Bioremediation of Trichloroethylene and Breakdown Products written by Jonathan R. Counts and published by . This book was released on 2020 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Trichloroethylene (TCE) is a persistent groundwater pollutant remaining from decades of use as a dry cleaning solvent and general degreaser. Having low solubility and a higher density than water, it often exists as a Dense Non-Aqueous Phase Liquid (DNAPL) at the bottom confining layer of aquifers, posing a unique remediation challenge. Bioremediation using anaerobic bacteria has proven successful, but high concentrations of TCE and proton buildup from degradation can incapacitate microbes, leaving the system "stuck". In addition, incomplete degradation of TCE results in by-products cis-1,2-dichloroethene (cDCE) and highly toxic vinyl chloride (VC). Encapsulation of microbes in a hydrogel bead to facilitate diffusion of pollutant and adsorption of acid may allow total remediation of problem systems. In this work, a series of membranes of various compositions are tested and diffusion coefficients determined for all species of interest using the diaphragm cell method. In Chapter 3, this information is used in a model to optimize hydrogel bead size and composition for bioremediation applications. Laboratory measurements of diffusion coefficients are time-consuming and may not be feasible for a diverse range of membrane types. It would be highly advantageous to build a correlation relating membrane properties to diffusivity. This would eliminate the need for repeated measurements using hazardous chemicals. Such a model would also allow rapid estimates of diffusion coefficients, expanding the likelihood of adoption of diffusion/reaction systems. Chapter 2 relates solute size, charge, and interactions within hydrogel membranes to steady state effective diffusivity for several molecules including hydrochloric acid. Increased understanding of the mechanisms of proton diffusion may have applications beyond bioremediation, including fuel cells and biomedical implants. Chapter 4 details future work which may improve efficacy of encapsulated cell systems.

Download or read book In Situ Bioremediation of Trichloroethylene Using Burkholderia Cepacia G4 PR1 written by Richard A. Snyder and published by . This book was released on 1998 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioaugmentation with Burkholderia Cepacia PR1301 for in Situ Bioremediation of Trichloroethylene Contaminated Water written by and published by . This book was released on 1998 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioremediation of Trichloroethylene by Indigenous Microbes in Groundwater written by Jennifer A. Humphries and published by . This book was released on 2003 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Spontaneous Decomposition of Trichloroethylene written by G. F. Yost and published by . This book was released on 1955 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Status Assessment of Toxic Chemicals written by J. C. Ochsner and published by . This book was released on 1980 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Distribution of Trichloroethylene and Geologic Controls on Contaminant Pathways Near the Royal River McKin Superfund Site Area Gray Maine written by and published by . This book was released on 1999 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Toxicological Profile for Trichloroethylene written by and published by . This book was released on 1995 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioremediation of Trichloroethylene Contaminated Sediments Augmented with a Dehalococcoides Consortia written by P. C. McKinsey and published by . This book was released on 2003 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the Department of Energy's (DOE) Savannah River Site (SRS) in Aiken, SC there are a number of sites contaminated with Chlorinated Ethenes (CE) due to past disposal practices. Sediments from two CE contaminated SRS locations were evaluated for trichloroethylene (TCE) biodegradation through anaerobic laboratory microcosms. The testing included addition of amendments and bioaugmentation of sediments. The anaerobic microcosms were first amended with substrates including acetate, lactate, molasses, soybean oil, methanol, sulfate, yeast extract, Regenesis HRC(R), and MEAL (methanol, ethanol, acetate, lactate mixture). Microcosms were analyzed after biostimulation for 9 months and no significant TCE biodegradation was observed. At 10 months, additional TCE, fresh amendments, and a mixed culture containing Dehalococcoides ethenogenes were added to active microcosms. A significant decrease in TCE concentrations and an increase in biodegradation products cis-dichloroethylene (cDCE) and vinyl chloride (VC) were noted within 2 weeks of bioaugmentation. Microcosms amended with lactate and sulfate showed complete transformation of TCE (3 ppm) to ethene within 40 days after bioaugmentation. Microcosms amended with other substrates - soybean oil, acetate, yeast extract, and methanol - also show enhanced biodegradation of TCE to ethene. Microcosms amended with molasses and Regenesis HRC showed limited TCE transformation. No TCE transformation was seen in killed control microcosms. On the basis of these successful results, plans are underway for field-scale in-situ deployment of biostimulation/bioaugmentation at SRS.

Download or read book Bioremediation of Trichloroethylene Using Wetland Mesocosms written by Michael Patrick Enright and published by . This book was released on 2001 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Model for Bioremediation of Trichloroethylene by Mixed Methanotrophic Culture Using Column Microcosm written by Shan He and published by . This book was released on 1995 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book In situ Bioremediation of Trichloroethylene Using Burkholderia Cepacia G4 PR1 written by J. R. Lawrence and published by . This book was released on 1998 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bio enhanced In well Vapor Stripping for the Remediation of Trichloroethylene Contamination written by Rahul K. Gandhi and published by . This book was released on 2001 with total page 866 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biostimulation of Trichloroethylene in Contaminated Aquifers written by William J. Hickey and published by . This book was released on 1999 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioremediation of Chlorinated and Polycyclic Aromatic Hydrocarbon Compounds written by Battelle Memorial In and published by CRC Press. This book was released on 1994-02-28 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: This timely reference presents the state of the art of the emerging and rapidly changing field of bioremediation of chlorinated solvents, PCBs, and other chlorinated compounds, as well as PAHs, both in situ and on site. This landmark publication reports significant advances in bioremediation, with an emphasis on practical applications and state-of-the-art developments. Laboratory and field-oriented reviews are presented with the objective of tying treatability studies and recent laboratory developments to field applications. No other reference source gives you access to the most current techniques and methods for the bioremediation of chlorinated and polycyclic aromatic hydrocarbon compounds. This book represents the work of leading experts in the fields of in situ and on-site bioremediation from North America, Europe, and Asia. The chapters include current field applications and laboratory studies undertaken, in some cases, in countries with regulatory standards more stringent than those of the United States.

Download or read book In Situ Bioremediation of Trichloroethylene contaminated Water by a Resting cell Methanotrophic Microbial Filter written by and published by . This book was released on 1992 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Lawrence Livermore National Laboratory is testing and developing an in situ microbial filter technology for remediating migrating subsurface plumes contaminated with low concentrations of trichloroethylene (TCE). Their current focus is the establishment of a replenishable bioactive zone (catalytic filter) along expanding plume boundaries by the Injection of a representative methanotrophic bacterium, Methylosinus trichosporium OB3b. We have successfully demonstrated this microbial filter strategy using emplaced, attached resting cells (no methane additions) in a 1.1-m flow-through test bed loaded with water-saturated sand. Two separate 24 h pulses of TCE (109 ppb and 85 ppb), one week apart, were pumped through the system at a flow velocity of 1.5 cm/h; no TCE (