Download or read book Biophysical Characterization of Functional Peptides written by Vibin Ramakrishnan and published by Springer Nature. This book was released on 2023-08-02 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.
Download or read book Biophysical Characterization of Bioactive Peptide Amphiphiles written by Ayala Fishel and published by . This book was released on 2000 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biophysical Characterization of Intrinsically Disordered Peptides Through Molecular Dynamics Simulations and Solution Nuclear Magnetic Resonance Spectroscopy written by David Jacob Rosenman and published by . This book was released on 2015 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Collagen Mimetic Peptides and Their Biophysical Characterization written by Jianxi Xiao and published by Springer. This book was released on 2024-09-21 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book embarks on an enlightening journey through the molecular landscape of collagen mimic peptides, shedding light on their significance and potential. In the intricate realm of biomolecules, collagen's preeminence as the most abundant protein in the human body, forming the foundational scaffolding of tissues and organs, renders it a subject of profound scientific interest. It explores diverse facets related to collagen mimic peptides, spanning aspects such as bioinformatic analysis, synthetic strategies, pathological collagen targeting, and the construction of homotrimeric, heterotrimeric and self-assembled peptide models. Furthermore, it provides an exhaustive investigation into biophysical techniques, encompassing Circular Dichroism (CD), X-ray crystallography, Nuclear Magnetic Resonance (NMR), Fluorescence, and Raman spectroscopy, thereby empowering researchers to unravel the structural intricacies of these peptides. The book unravels the profound implications of collagen mimic peptides across a spectrum of scientific domains, including protein science, biomaterials, bioanalysis, and beyond. Its accessibility and insights cater not only to seasoned researchers but also to undergraduate and graduate students eager to delve into the complexities of this fundamental protein.
Download or read book Protection of Functional Groups in Peptide Synthesis written by Erhard Gross and published by Elsevier. This book was released on 2014-05-10 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Peptides: Analysis, Synthesis, Biology, Volume 3: Protection of Functional Groups in Peptide Synthesis focuses on protection of functional groups in peptide synthesis. This book consists of seven chapters. Chapter 1 reviews the large variety of amine protecting groups. The protection of carboxyl groups is described in Chapter 2, while the chemistry of sulfhydryl group protection in peptide synthesis is discussed in Chapter 3. Chapter 4 covers the protection of the hydroxyl groups of serine, threonine, tyrosine, and other hydroxyl-containing amino acids. Differential protection and selective deprotection in peptide synthesis is deliberated in Chapter 5. In chapter 6, the opportunities and constraints of the tactics of minimal protection of side-chain functions during peptide synthesis are reviewed. The last chapter is devoted to the interesting aspects of dual function groups. This volume is recommended for specialists and researchers concerned with peptide and protein research.
Download or read book Biophysical Characterisation of Peptides and Proteins written by Angeliki Damianoglou and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stability and Characterization of Protein and Peptide Drugs written by Rodney Pearlman and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first volume to make available specific case histories of therapeutic proteins and peptides that have been marketed or are currently under clinical testing. The editors have selected a wide range of molecules derived from monoclonal antibodies, recombinant DNA, and natural and chemical sources to provide formulation scientists with practical examples of the development of pharmaceutical products.
Download or read book Towards a Better Understanding of Membrane Proteins written by T. Meijneke and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Peptides Analysis Synthesis Biology written by Erhard Gross and published by Elsevier. This book was released on 2012-12-02 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides comprehensive and critical reviews of important developments in all areas of peptide research including analysis, synthesis, and biology. X-ray structure studies, amino acid analysis, and chiroptical analysis of configuration are discussed, along with solid-phase sequencing and ultramicroanalysis with the aid of fluorescence. This volume is comprised of six chapters and begins with an account of crystal structure analysis on molecules containing 2-12 peptide units, focusing on the variety of intramolecular hydrogen bonds, cis peptide units, and multiple conformation. Conformational changes upon complexation with metal ions are considered, together with the inclusion of solvents as integral parts of a molecular structure. The following chapters explore the conformations of insulin, glucagon, pancreatic polypeptide and related molecules, as well as the molecular biology of these hormones based on crystal structures; the usefulness of chiroptical techniques for determining the absolute configuration of amino acids and small peptides; and ultramicroanalysis of peptides and proteins by high performance liquid chromatography and fluorescence detection. The final chapter looks at the status and future potential of solid-phase sequencing. This book is intended as a reference for specialists, a guide for the novice, and a forum for investigators concerned with research on peptides and proteins.
Download or read book Rational Development and Biophysical Analysis of Medically relevant Peptides written by Erika J. Olson and published by . This book was released on 2017 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Synthesis and Biophysical Characterisation of Cationic Amphipathic PH Responsive Gene Delivery Peptides Containing Primary amine 2 3 diaminopropionic Acid Residues and Secondary amine Mono methylated Derivatives written by Jayneil Patel and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Delivery of therapeutic oligonucleotide sequences by cationic amphipathic histidine rich peptides like LAH4-L1 is modulated by peptide response to pH changes during endocytosis. pH sensitivity can be affected by the number of histidine residues, defining its transfection efficiency. Optimizing the gene delivery efficiencies of LAH4-L1 by increasing the number of histidine residues and therefore peptide charge is limited by the Coulombic interactions that stabilise a-helical self-associated peptide oligomers. Consequently, peptides containing six histidine residues function at a pH below that which occurs in the endosome, decreasing gene delivery capabilities. This limitation is expected to be overcome by substituting histidine residues with the more basic pH response 2,3- diaminopropionic acid (Dap). It is predicted that peptides containing 6 Dap residues will remain sensitive to pH changes observed in the endosome, leading to more efficient gene delivery peptide. To test these hypothesis peptides containing four and six Dap residues were synthesised. Analogously, derivatives containing mono-methylated P-amine Dap residues were formed in attempt to reduce cytotoxicity associated with primary amine peptides. Biophysical analysis was performed to determine the peptides pH response in key mechanisms that occur during gene delivery. It is shown that Dap residues can balance the Coulombic effects and respond at a more basic pH than histidine containing analogues. Whereas Dap substitution does not give increased gene delivery compared to LAH4-L1; mono-methylated Dap variants remain sensitive to pH changes in endocytosis and show increased gene delivery efficiencies compared to LAH4-L1. Peptides containing six mono-methylated Dap residues gave transfection results reproducible in serum greater than LAH4-L1 and comparable to commercially available peptide vectors.
Download or read book Synthesis and Biophysical Characterization of Cationic Amphipathic PH Responsive Gene Delivery Peptides Containing Primary Beta amine 2 3 diaminopropionic Acid Residues and Secondary Beta amine Mono methylated Derivatives written by Jayneil Patel and published by . This book was released on 2011 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Delivery of therapeutic oligonucleotide sequences by cationic amphipathic histidine rich peptides like LAH4-L1 is modulated by peptide response to pH changes during endocytosis. pH sensitivity can be affected by the number of histidine residues, defining its transfection efficiency. Optimizing the gene delivery efficiencies of LAH4-L1 by increasing the number of histidine residues and therefore peptide charge is limited by the Coulombic interactions that stabilise a-helical self-associated peptide oligomers. Consequently, peptides containing six histidine residues function at a pH below that which occurs in the endosome, decreasing gene delivery capabilities. This limitation is expected to be overcome by substituting histidine residues with the more basic pH response 2,3- diaminopropionic acid (Dap). It is predicted that peptides containing 6 Dap residues will remain sensitive to pH changes observed in the endosome, leading to more efficient gene delivery peptide. To test these hypothesis peptides containing four and six Dap residues were synthesised. Analogously, derivatives containing mono-methylated P-amine Dap residues were formed in attempt to reduce cytotoxicity associated with primary amine peptides. Biophysical analysis was performed to determine the peptides pH response in key mechanisms that occur during gene delivery. It is shown that Dap residues can balance the Coulombic effects and respond at a more basic pH than histidine containing analogues. Whereas Dap substitution does not give increased gene delivery compared to LAH4-L1; mono-methylated Dap variants remain sensitive to pH changes in endocytosis and show increased gene delivery efficiencies compared to LAH4-L1. Peptides containing six mono-methylated Dap residues gave transfection results reproducible in serum greater than LAH4-L1 and comparable to commercially available peptide vectors.
Download or read book The Design and Characterization of Functional Amphiphilic Peptides written by Shlomo Zarzhitsky and published by . This book was released on 2014 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanoscale Assembly written by Wilhelm T.S. Huck and published by Springer Science & Business Media. This book was released on 2006-07-11 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology has received tremendous interest over the last decade, not only from the scientific community but also from a business perspective and from the general public. Although nanotechnology is still at the largely unexplored frontier of science, it has the potential for extremely exciting technological innovations that will have an enormous impact on areas as diverse as information technology, medicine, energy supply and probably many others. The miniturization of devices and structures will impact the speed of devices and information storage capacity. More importantly, though, nanotechnology should lead to completely new functional devices as nanostructures have fundamentally different physical properties that are governed by quantum effects. When nanometer sized features are fabricated in materials that are currently used in electronic, magnetic, and optical applications, quantum behavior will lead to a set of unprecedented properties. The interactions of nanostructures with biological materials are largely unexplored. Future work in this direction should yield enabling technologies that allows the study and direct manipulation of biological processes at the (sub) cellular level.
Download or read book Bioactive Peptides from Food written by Leo M.L. Nollet and published by CRC Press. This book was released on 2022-03-28 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: A growing body of scientific evidence has revealed that many food peptides exhibit specific biological activities in addition to their established nutritional value. Bioactive peptides present in foods may help reduce the worldwide epidemic of chronic diseases that account for a great number of premature deaths annually. Bioactive peptides can be defined as isolated small fragments of proteins which provide some physiological health benefits. They act as potential modifiers reducing the risk of many chronic diseases. Bioactive Peptides from Food: Sources, Analysis, and Functions considers fundamental concepts, sources, hydrolysis, fractionation, purification, analysis, chemical synthesis, functions, and regulatory status of nutraceutical bioactive peptides. Methods of isolation of these peptides from different protein sources with their in vitro and vivo physiological effects are addressed. Divided into seven sections, this book delves into how these peptides play a major role in the development of various functional foods. Numerous bioactive peptides have been reported in recent years as naturally present or generated from food proteins of different origins like milk, eggs, soya, fish, and meat. Key Features: Includes a detailed study of the different sources of bioactive peptides Discusses the health benefits, such as antimicrobial, antiallergic, antihypertensive, antitumor, and immunomodulatory properties of peptides Explorates the state of the art analysis methods of peptides Discovers the bioinformatics of possible bioactive peptides Written by experts in their field from around the world, Bioactive Peptides from Food reveals the world of databases of peptides. It is a great resource for food scientists, technologists, chemists, nutrition researchers, producers, and processors working in the whole food science and technology field as well as those who are interested in the development of innovative functional products.
Download or read book Peptide Characterization and Application Protocols written by Gregg B. Fields and published by Humana Press. This book was released on 2010-11-19 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.
Download or read book Peptides written by Norbert Sewald and published by John Wiley & Sons. This book was released on 2015-11-19 with total page 771 pages. Available in PDF, EPUB and Kindle. Book excerpt: Der "Sewald/Jakubke" ist und bleibt das einzige moderne, auf dem neuesten wissenschaftlichen Stand gehaltene fortgeschrittene Lehrbuch der Peptid-Biochemie! Auch für diese zweite, um ein Viertel erweitere Auflage haben die Autoren Hunderte von Publikationen gelesen und zu einem informativen und vollständigen Überblick über das Fachgebiet zusammengefasst. Nach einer Einführung in die Grundlagen der Peptidstruktur und -synthese werden die wichtigsten Familien biologisch aktiver Peptide systematisch abgehandelt. Mit interdisziplinärem Anspruch werden dann wichtige Anwendungen aus der Biotechnologie, Pharmazie und Biomedizin besprochen. Selbstverständlich werden auch Themen der Spitzenforschung berücksichtigt, zum Beispiel Pseudopeptide, Peptidmimetika und Aspekte der kombinatorischen Synthese. -- Neu in dieser Auflage sind Fragen und Übungsaufgaben, die das Nacharbeiten von Vorlesungen oder das Selbststudium erleichtern.
