Download or read book Biomolecular Patterning on Micro and Nanofabricated Surfaces for Biosensor Applications and Cell based Research written by Reid Nelson Orth and published by . This book was released on 2003 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this study, biomaterials are patterned at the micro- and nanometer scale for biosensor applications and cell-based research. Two enabling technologies were photoactivatable biotin and the polymer lift-off technique. Avidin-biotin complexes have been immobilized on planar silicon substrates and to the inner surfaces of capillary tubes. The latter serves as a model for patterning inside microfluidic systems. Several biomaterials have been patterned using the polymer lift-off method, including lipids, metal, microspheres, biotin, and proteins (e.g., antibodies, protein A, and NeutrAvidin). The patterned lipids formed supported lipid bilayers with diffusion coefficients comparable to cell membranes as confirmed by fluorescence recovery after photobleaching (FRP). The author's tailored patterns comprised 2, 4-dinitrophenyl (DNP) or biotin functionalized lipids with features ranging from 1.3 micrometers to 76 micrometers. Non-lipid molecules were patterned in features as small as 700 nanometers. A characterization of the surfaces has shown a highly uniform patterning. Several of these materials also were applied in patterned regions inside microtrenches. Micro- and nanometer-scale patterns of functionalized biomaterials served as spatially controlled stimuli for cell surface ligands in this research. This technology has been applied to rat basophilic leukocyte (RBL) cells, eosinophils, lymphocytes, macrophages, and fibroblasts. For RBL experiments, DNP-conjugated lipids have been incorporated in the lipid bilayers with fluorescent lipid DiIC16 and used to stimulate RBL cells sensitized with anti-DNP IgE on the Fc receptors. Receptor aggregation was observed with confocal microscopy. This technique allows researchers to study the effects of antigen density on cellular response, binding kinetics, and the redistribution after individual binding events. (1 table, 71 figures).
Download or read book Surface Patterned Nano and Microarrays Using Parylene for Biological Applications written by Christine Pek Boey Tan and published by . This book was released on 2011 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spatial patterning of biomolecules on a surface with nano- and micrometer precision is important in engineering biological microenvironments. Nano- and micropatterned biological arrays are useful for chemical functionalization of miniaturized biosensors, tissue engineering, high throughput drug screening, and fundamental biophysical and molecular biology studies. Despite the growing demands and applications, current surface patterning techniques are still lacking. Parylene (or poly[p-xylylene]) is a unique family of chemically vapor deposited polymer that is biocompatible, exhibits little to no swelling in aqueous solutions and is amenable to photolithography processing. These characteristics of parylene are attractive for its use as a "peel-able", high fidelity stencil for the surface patterning of biomolecular arrays. Different types of parylene coatings containing substituted functional groups have been developed for biochemical surface modification on virtually any surface. The two themes that emerge from this work are: i) the use of parylene films with nano/microfabricated openings as a stencil tool for surface patterning (Chapters 2 - 4), and ii) the application of parylene coatings to directly pattern surfaces (Chapters 5 and 6). Chapter 2 describes a new nanofabrication process to create parylene stencils with sub-100nm openings. These stencils can then be combined with inkjet printing to rapidly generate multi-component biomolecular nanoarrays on a single surface. The work in Chapter 3 elucidates the role of cell-cell interactions in tumor angiogenesis through the use of patterned tumor cell arrays. In Chapter 4, a hybrid substrate, consisting of a hydrophobic parylene stencil with hydrophilic silanized openings, is utilized to control spot drying morphology and improve microarray reproducibility. Parylene-based paper microfluidic devices and their enhanced capabilities are presented in Chapter 5. The work in Chapter 6 involves proteins covalently immobilized onto an array of reactive (aminated) parylene strips. These strips subsequently incorporated with microfluidics for the affinity-based screening and subsequent recovery of aptamers. Chapter 7 is a summary containing unpublished observations of parylene that may serve as a useful starting point for future researchers. These cumulative studies demonstrate that parylene-based surface patterning techniques are versatile for creating nano- and microarrays towards many biological applications.
Download or read book Surface Design Applications in Bioscience and Nanotechnology written by Renate Förch and published by John Wiley & Sons. This book was released on 2009-08-06 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: This carefully selected balance of tutorial-like review chapters and advanced research covers hot topics in the field of biointerfaces, biosensing, nanoparticles at interfaces, and functionalized quantum dots. It also includes chapters arising from non-published work with topics such as surface design and their applications, as well as new developments in analytical tools for materials science and life science. Based on the very close and complementary collaboration of three distinguished leading research groups, this book highlights recent advances in the field ranging from synthesis and fabrication of organic and polymeric materials, surface and interface science to advanced analytical methods. It thus addresses new concepts in micro- and nanofabrication, bio-nanotechnology, biosensors and the necessary compositional and structural analysis. Particular attention is paid throughout to complex hierarchical interface architectures and possible applications of the chemical and physical methodologies discussed, covering bio-diagnostics, novel biosensors and adhesion science. With its unique combination of expertise from chemistry, physics, biology, surface science and engineering, this is a valuable companion for students, practitioners and established experts.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2003 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Patterning Biomolecules Onto Surfaces for Biosensor Applications written by André Bernard (dr en biochimie.) and published by . This book was released on 1998 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomolecular Patterning in Microfabricated Systems with Avidin biotin and Microcontact Printing written by Reid Nelson Orth and published by . This book was released on 2001 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Patterning Biomolecules Onto Surfaces for Biosensor Applications written by André Bernard and published by . This book was released on 1998 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book American Doctoral Dissertations written by and published by . This book was released on 2002 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Construction of Versatile Biomolecule Nano platforms Via Dip pen Nanolithography and Their Application in Bio sensing and Cell Differentiation written by Sabine Oberhansl and published by . This book was released on 2012 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present thesis entitled "Construction of versatile biomolecule nano-platforms via Dip-pen Nanolithography and their application in bio-sensing and cell differentiation" aims at contributing to the field of Nanobiotechnology, best defined as miniaturized biotechnology where nanofabrication technology is used for biological purposes. To this end, this research work employs the relatively novel nanofabrication technique called Dip-pen Nanolithography (DPN) for direct patterning of biologically relevant molecules at the micro- and nanoscale, both for biosensor applications and cell differentiation studies. - The first chapter deals with the challenges encountered when working with Dip-pen Nanolithography and so called multi-pens. In order to facilitate the levelling of the tips with respect to the substrate and also to obtain a constant feedback of the force exerted from the tips to the substrate, a device was developed and fabricated together with a collaboration. The resulting piezoresistive device could be implemented successfully and was used to pattern two different molecules on gold substrates. An improved sensitivity at detecting the touching point could be shown when compared to conventional tips. - The second chapter deals with the miniaturization of an already in-house developed biosensor platform. Therefore, several different approaches were evaluated for the DPN patterning of oligonucleotides on gold or chemically modified glass in order to ensure a covalent attachment. The most successful strategy was the click chemistry route, where an azide bearing oligonucleotide was coupled to an alkyne bearing glass substrate, using a copper catalyst (1,3-dipolar cycloaddition) yielding an triazole. This way, a sensor platform could be established and the sensitivity could be assessed. - The third chapter deals with the development and fabrication of DPN patterned substrates for cell differentiation experiments. A biotin-thiol was chosen for patterning because it allows derivatization with streptavidin and any type of biotinylated molecule. The patterning was accomplished, using a lipid as a carrier molecule. This way, the feature size of the pattern was around 5 μm and the overall pattern area was bigger than 1 mm2. The substrates were applied successfully in cell differentiation experiments, having a biotinylated BMP-2 immobilized on the substrates.
Download or read book Micro and Nano chemical Patterning of Surfaces for Biological Applications written by Roger Michel and published by . This book was released on 2002 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Patterning of Biomolecules on Plasma Enhanced Chemical Vapor Deposited Generated Surfaces written by and published by . This book was released on 2006 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micropatterned surfaces have received extensive attention for possible applications in advanced technologies including microelectronics, microfluidics, cell growth confinement and biosensor fabrication. The latter two applications exemplify the increasing coordination between materials science and biology for future generation advanced materials. Plasma-enhanced chemical vapor deposition (PECVD) shows great promise for strengthening this aforementioned materials science and biology intersection. PECVD provides an excellent generalized platform for the incorporation of a wealth of different biomolecules and/or biologically inspired materials by way of micropatterned structures. Micropatterned substrates with site-specific binding were developed by way of self-assembled monolayer chemistry in conjunction with thin layer organic polymer deposition via PECVD. Spatial binding of biomolecules and quantum dots to PECVD patterned substrates are demonstrated.
Download or read book Biosensing Using Nanomaterials written by Arben Merkoci and published by John Wiley & Sons. This book was released on 2009-03-25 with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt: An interdisciplinary approach to one of the hottest topics in nanotechnology and nanoscience Biosensing Using Nanomaterials introduces novel concepts in the area of bioanalysis based on nanomaterials, opening new opportunities for basic research and new tools for real bioanalytical applications. In fifteen chapters, readers are introduced to the most successful nanomaterials used so far in biosensing, including carbon nanotubes, nanoparticles, and nanochannels. Each chapter provides a theoretical overview of the topic, a discussion of the published data relating to the bioanalytical system, and a selected list of references for further investigation. The result is a book that provides a comprehensive forum of interest to scientists, engineers, researchers, manufacturers, teachers, and students. Biosensing Using Nanomaterials is an important resource for a broad audience involved in the research, teaching, learning, and practice of integrating nanomaterials into biosensing systems for clinical, environmental, and industrial applications.
Download or read book Micro and Nano Bionic Surfaces written by Deyuan Zhang and published by Elsevier. This book was released on 2021-11-02 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro- and Nano-Bionic Surfaces: Biomimetics, Interface Energy Field Effects, and Applications synthesizes the latest research in bio-inspired surfaces and devices for tactile and flow field perception. The book provides solutions to common problems related to flow field/tactile perception, intelligent MEMS sensors, smart materials, material removal methods, cell/particle control methods, and micro-nano robot technology. With a heavy emphasis on applications throughout, the book starts by providing insights into biomimetic device design, outlining strategies readers can adopt for various engineering applications. From there, it introduces the controlling methods of smart materials, controlling methods from external energy input, and more. Sections demonstrate how to solve problems of high efficiency, high quality, and low damage material removal for metals, composites, soft tissues, and other materials by applying bionic wave-motion surface characteristics. The latest theoretical and technical developments in field control methods applied to biological interfaces are also discussed, and the book concludes with a chapter on fabrication strategies to synthesize micro/nano functional particles based on bio-templates. Provides an overview on the latest research in bio-inspired surfaces and devices for tactile and flow-field perception Introduces techniques for characterizing different bionic surfaces and how to use energy fields analysis to treat different bionic surface and interface problems Discusses the latest theoretical and experimental developments in field control and their applications in the biomedical field Outlines fabrication methods and assembly and alignment processes of micro-/nano-functional particles based on microorganism templates
Download or read book Proceedings of Micro and Nanofabricated Electro optical Mechanical Systems for Biomedical and Environmental Applications written by Society of Photo-optical Instrumentation Engineers and published by SPIE-International Society for Optical Engineering. This book was released on 1997 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanomaterials for Biosensors written by Bansi D. Malhotra and published by William Andrew. This book was released on 2017-10-26 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials for Biosensors: Fundamentals and Applications provides a detailed summary of the main nanomaterials used in biosensing and their application. It covers recent developments in nanomaterials for the fabrication of biosensor devices for healthcare diagnostics, food freshness and bioprocessing. The various processes used for synthesis and characterization of nanostructured materials are examined, along with the design and fabrication of bioelectronic devices using nanostructured materials as building blocks. Users will find the fundamentals of the main nanomaterials used in biosensing, helping them visualize a systematic and coherent picture of how nanomaterials are used in biosensors. The book also addresses the role of bio-conjugation of nanomaterials in the construction of nano-biointerfaces for application in biosensors. Such applications, including metal nanoparticles, metal oxide nanoparticles, nanocomposites, carbon nanotubes, conducting polymers and plasmonic nanostructures in biosensing are discussed relative to each nanomaterial concerned. Finally, recent advancements in protein functionalized nanomaterials for cancer diagnostics and bio-imaging are also included. Provides a detailed study on how nanomaterials are used to enhance sensing capabilities in biosensors Explains the properties, characterization methods and preparation techniques of the nanomaterials used in biosensing Arranged in a material-by-material way, making it clear how each nanomaterial should be used
Download or read book Intelligent Surfaces in Biotechnology written by H. Michelle Grandin and published by John Wiley & Sons. This book was released on 2012-02-28 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive overview of smart and responsive surfaces in biotechnology and their applications A wave of recent advances in cell biology, biophysics, chemistry, and materials science has enabled the development of a new generation of smart biomaterials. Intelligent Surfaces in Biotechnology: Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications provides readers with a comprehensive overview of surface modifications and their applications, including coverage of the physico-chemical properties, characterization methods, smart coating technologies, and demonstration of performance in vitro and in vivo. The first part of the book covers applications in the fields of biosensing and biodiagnostics, while the second part focuses more on coatings for medical devices, drug delivery, and tailored cell-surface interactions. The book explores intelligent surface applications such as tissue engineering, drug targeting and delivery, wound healing and anti-infection strategies, biosensors, nanopatterning, and bioinspired design of novel responsive materials and multifunctional surfaces. Designed to aid scientists and engineers in understanding the rapidly developing field of biofunctional surfaces, Intelligent Surfaces in Biotechnology is an edited volume with each chapter written by a respected expert and featuring examples taken from the most state-of-the-art developments in the discipline. Cover Image: Design concept for a diagnostic microfluidic system based on responsive polymer- and antibody-conjugated nanobeads (see Chapter 2 of this book, Figure 2.5; reproduced by permission from the Royal Society of Chemistry).
Download or read book Nanoplasmonic Sensors written by Alexandre Dmitriev and published by Springer Science & Business Media. This book was released on 2012-07-31 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compendium of the finest research in nanoplasmonic sensing done around the world in the last decade. It describes basic theoretical considerations of nanoplasmons in the dielectric environment, gives examples of the multitude of applications of nanoplasmonics in biomedical and chemical sensing, and provides an overview of future trends in optical and non-optical nanoplasmonic sensing. Specifically, readers are guided through both the fundamentals and the latest research in the two major fields nanoplasmonic sensing is applied to – bio- and chemo-sensing – then given the state-of-the-art recipes used in nanoplasmonic sensing research.
