Download or read book Biology of Negative Strand RNA Viruses The Power of Reverse Genetics written by Yoshihiro Kawaoka and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Negative-strand RNA viruses, so named because of the polarity of their genomic RNA to mRNA, include important human and non-human pathogens. This volume covers major advances in reverse genetics techniques over the past decade, state-of-the-art basic science and the clinical implications of experimental findings. This should rekindle interest in negative-strand RNA viruses among readers, including those in other disciplines, leading to further progress in understanding these important viruses and in developing effective measures of control.
Download or read book Reverse Genetics of RNA Viruses written by Anne Bridgen and published by John Wiley & Sons. This book was released on 2012-10-07 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases. Reverse Genetics of RNA Viruses provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field. An authoritative, comprehensive overview of reverse genetics in RNA Viruses. Includes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. Written by a team of international experts, including some of the leading researchers in the field.
Download or read book Negative Strand RNA Virus written by Ming Luo and published by World Scientific. This book was released on 2011 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Negative strand RNA viruses have a unique mechanism of replication. Their genome is a single strand RNA that has to be transcribed as soon as the virus enters the host in order to carry out viral replication. As a result, a viral-specific RNA polymerase is packaged in the virion and is ready for transcription after virus entry. This novel replication mechanism dictates the assembly and RNA synthesis of negative strand RNA viruses. In recent years, many discoveries have been made with regard to the entry, replication and assembly of this class of viruses. This book will present updated coverage of the fundamental knowledge about negative strand RNA viruses.
Download or read book Reverse Genetics of RNA Viruses written by Daniel R. Perez and published by Humana. This book was released on 2018-06-24 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Reverse Genetics of Negative Stranded RNA Viruses written by Fundación Juan March. Instituto de Estudios e Investigaciones and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Genetics written by Esteban Domingo and published by CRC Press. This book was released on 2018-02-06 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: First Published in 2018. Routledge is an imprint of Taylor & Francis, an Informa company.
Download or read book Viruses and Human Disease written by Ellen G. Strauss and published by Elsevier. This book was released on 2007-09-21 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and updated, the new edition of this groundbreaking text integrates basic virology with pathophysiological conditions to examine the connection between virology and human disease. Most virology textbooks focus on the molecular biology involved without adequate reference to physiology. This text focuses on viruses that infect humans, domestic animals and vertebrates and is based on extensive course notes from James Strauss’ virology class at the California Institute of Technology taught for over 30 years. Expertly depicting in color the molecular structure and replication of each virus, it provides an excellent overview for students and professionals interested in viruses as agents of human disease. Includes over 30% new material - virtually all of the figures and tables have been redrawn to include the latest information and the text has been extensively rewritten to include the most up-to-date information Includes a new chapter on emerging and reemerging viral diseases such as avian flu, SARS, the spread of West Nile virus across America, and the continuing spread of Nipah virus in Southeast Asia Further reading sections at the end of each chapter make it easy find key references World maps depicting the current distribution of existing and newly emerging viruses are also incorporated into the text
Download or read book Avian Metapneumovirus Studies Using Reverse Genetics written by Marco Falchieri and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Avian metapneumovirus (AMPV) is an enveloped negative sense single stranded RNA virus which is a major endemic respiratory pathogen of global domestic poultry. Since reverse genetic (RG) techniques have been applied to this pathogen several reports have investigated the effects of single and multiple genomic mutations and gene deletions or insertions on viral biology. The aim of this study was to gain a better understanding of the viral capacity to accept and in some cases express homologous and heterologous extra sequences. Initially an AMPV subtype A was modified to introduce a homologous 200 bp sequence within the G gene and this recombinant was suggested to be used as a positive control for validating all stages of a previously established RT-nested PCR. Different Green Fluorescent Protein (GFP) AMPV recombinants were then prepared each one containing the reporter gene in a different intergenic position and then were assessed for expression, stability and viability. In particular, quantification of the expression was calculated using a quantitative ELISA. All recombinants showed high stability, while good viability was observed in all the positions, except when GFP was inserted between nucleocapsid (N) and phosphoprotein (P). The highest expression was detected in the virus with the insertion between N and P as expected, according to the transcriptional model for non-segmented negative stranded viruses. However GFP was produced at high levels even when inserted at the trailer end intergenic positions. Poor expression was seen for all the other positions. The vectoring abilities of subtype A strains were further investigated to accept and express foreign genes, specifically GFP gene and both spike (S1) and nucleocapsid (N) genes of infectious bronchitis virus (IBV). After viruses had been recovered by RG, all recombinants were proven to express the inserted genes efficiently and were all found to be highly stable during passage in vitro. Subsequently IBV recombinants were tested as candidate vaccines by eye-drop inoculation of one-day-old chickens. When chicks were challenged with IBV, partial protection results were observed, as assessed by greater motility of tracheal cilia from animals receiving the recombinants. Finally the development of a new RG system was attempted in order to extend this type of studies to the B subtype. This subtype is distributed worldwide and growing field evidence suggests it to be more able to infect commercial chickens compared to subtype A. For this reason it would be convenient to have an RG available also for B viruses. The construction of a DNA copy of the viral genome was attempted using site-directed mutagenesis and ligation techniques, resulting in more than 85% of the genome cloned. Unfortunately full genome cloning proved to be not possible, as severe problems were encountered, including construct instability and cloning bacteria intolerance to viral sequences.
Download or read book RNA Viruses written by Decheng Yang and published by World Scientific. This book was released on 2009 with total page 722 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first comprehensive book on human/animal gene responses to RNA viral infections, including prevalent, emerging and re-emerging RNA viruses such as HIV, SARS-CoV, West Nile virus, influenza virus and many others. Human gene responses are reviewed by leading virologists worldwide in the following aspects: (i) the altered gene expression profiles at the transcriptional and translational levels detected with cutting-edge technologies such as cDNA microarray and proteomics; (ii) host innate and adapted immune responses to viral replication in target organs; (iii) virus-activated signal transduction pathways in cell survival, apoptosis and autophagosomal pathways; and (iv) the small interfering RNA/microRNA-mediated gene silencing pathway, a recently characterized new host defense mechanism against viral infection. Organized into 29 highly accessible and well-illustrated chapters, this volume explores state-of-the-art knowledge of the molecular mechanisms of RNA virus infection and host?virus interactions. This comprehensive compilation of the altered gene expression profiles and signal transduction pathways in host cells in response to the majority of human/animal RNA viruses opens new directions for basic and clinical research on viral pathogenesis, and also provides valuable biomarkers for researchers to select gene targets in the development of diagnostic tests and antiviral therapeutics for a number of infectious diseases.
Download or read book Principles of Molecular Virology written by Alan Cann and published by Elsevier. This book was released on 2005-07-26 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Principles of Molecular Virology, Fourth Edition" provides an essential introduction to modern virology in a clear and concise manner. It is a highly enjoyable and readable text with numerous illustrations that enhance the reader's understanding of important principles. It contains new material on virus structure, virus evolution, zoonoses, bushmeat, SARS and bioterrorism. The standard version includes a CD-ROM with Flash animations, virtual interactive tutorials and experiments, self-assessment questions, useful online resources, along with the glossary, classification of subcellular infectious agents and history of virology.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Multiplication of RNA Plant Viruses written by Chundi L. Mandahar and published by Springer Science & Business Media. This book was released on 2006-09-05 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical studies on plant virus RNA replication have advanced considerably since 2000, primarily because of new genetic, molecular, biochemical, and enzymatic studies. This book generates understanding of multiplication of plus-sense RNA plant viruses, especially at molecular level. Certain virus-encoded essential proteins, nucleotide sequence motifs, and RNA secondary structures are central to virus RNA replication, which has a number of stages. Each is a complex phenomenon requiring specific factors and conditions.
Download or read book Replication Competent Reporter Expressing Viruses written by Luis Martinez-Sobrido and published by MDPI. This book was released on 2018-09-27 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Replication-Competent Reporter-Expressing Viruses" that was published in Viruses
Download or read book Molecular Virology written by Susanne Modrow and published by Springer. This book was released on 2013-09-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.
Download or read book The Bunyaviridae written by Richard M. Elliott and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this comprehensive reference, leading researchers examine the biology, molecular biology, and diseases of the Bunyaviridae, and provide up-to-date information on the genetic characterization and variations of this virus group. Chapters deal with the molecular biology of five genera: Bunyavirus, Hantavirus, Nairovirus, Phlebovirus, and Tospovirus. Chapters examine Bunyaviridae assembly and intracelluar protein transport as well as Bunyaviridae genetics. Contributors discuss the Bunyaviridae diseases, including the hantavirus pulmonary syndrome.
Download or read book New Advances on Zika Virus Research written by Luis Martinez-Sobrido and published by MDPI. This book was released on 2019-04-02 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zika virus (ZIKV) is a mosquito-borne member of the Flaviviridae family that historically has been associated with mild febrile illness. However, the recent outbreaks in Brazil in 2015 and its rapid spread throughout South and Central America and the Caribbean, together with its association with severe neurological disorders—including fetal microcephaly and Guillain-Barré syndrome in adults—have changed the historic perspective of ZIKV. Currently, ZIKV is considered an important public health concern that has the potential to affect millions of people worldwide. The significance of ZIKV in human health and the lack of approved vaccines and/or antiviral drugs to combat ZIKV infection have triggered a global effort to develop effective countermeasures to prevent and/or treat ZIKV infection. In this Special Issue of Viruses, we have assembled a collection of 32 research and review articles that cover the more recent advances on ZIKV molecular biology, replication and transmission, virus–host interactions, pathogenesis, epidemiology, vaccine development, antivirals, and viral diagnosis.
Download or read book Virus as Populations written by Esteban Domingo and published by Academic Press. This book was released on 2019-11-06 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Virus as Composition, Complexity, Quasispecies, Dynamics, and Biological Implications, Second Edition, explains the fundamental concepts surrounding viruses as complex populations during replication in infected hosts. Fundamental phenomena in virus behavior, such as adaptation to changing environments, capacity to produce disease, and the probability to be transmitted or respond to treatment all depend on virus population numbers. Concepts such as quasispecies dynamics, mutations rates, viral fitness, the effect of bottleneck events, population numbers in virus transmission and disease emergence, and new antiviral strategies are included. The book's main concepts are framed by recent observations on general virus diversity derived from metagenomic studies and current views on the origin and role of viruses in the evolution of the biosphere. Features current views on key steps in the origin of life and origins of viruses Includes examples relating ancestral features of viruses with their current adaptive capacity Explains complex phenomena in an organized and coherent fashion that is easy to comprehend and enjoyable to read Considers quasispecies as a framework to understand virus adaptability and disease processes
