Download or read book Chemical Reagents for Protein Modification Fourth Edition written by Roger L. Lundblad and published by CRC Press. This book was released on 2014-07-22 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2018-01-18 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers. As a result, little emphasis is given to the individual consideration of the functional consequence of chemical modification.
Download or read book Chemical Reagents for Protein Modification written by ROGER L. LUNDBLAD and published by CRC Press. This book was released on 2021-03-30 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive review of reagents used for the chemical modification of proteins, the fourth edition represents a major revision. The completely updated text is substantially larger and includes five new chapters: Alkylating Agents, Acylating Agents, Nitration and Nitrosylation, Oxidation, and Modification of Proteins with Reducing Agents. The
Download or read book Photogenerated Reagents in Biochemistry and Molecular Biology written by H. Bayley and published by Elsevier. This book was released on 2000-04-01 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is an extended account of a group of techniques that have seen their importance realized in almost all areas of biochemistry. The book provides detailed description of the properties and syntheses of the most useful photoactivatable reagents as well as comprehensive and critical descriptions of the major experiments that can be performed with photochemical reagents, affinity labelling, cross-linking of macromolecules and topographical analyses of membrane proteins.
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2020-03-11 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
Download or read book Dry Chemistry written by O. Sonntag and published by Elsevier. This book was released on 1993-10-14 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dry chemistry has been accepted as an important technology in medical laboratories for many years. Many evaluations of this technology have been undertaken by reputable clinical laboratories, the results of which were excellent when compared with conventional wet chemistry analysis. This book contains a detailed overview of the current knowledge in the field of dry chemistry both in the physicians' office laboratories and large medical laboratories. The results from many evaluation studies are presented, as is data from interference studies which complete the descriptions of many dry chemistry methods. A detailed description of various commercially available dry chemistry systems such as Ektachem, Reflotron, Seralyzer, Cobas Ready, Drichem, Opus and Stratus are also included. This book effectively describes the current state-of-the-art technology and knowledge and succeeds in filling the gap in information in this important field of clinical chemistry science. Originally published as 'Trockenchemie' by Georg Thieme Verlag,Stuttgart, Dr. Sonntag has taken the opportunity of this translation to completely revise and update the contents of his book.
Download or read book Linscott s Directory of Immunological and Biological Reagents written by and published by . This book was released on 1996 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2018-01-18 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers.
Download or read book Linscott s Directory of Immunological and Biological Reagents written by [Anonymus AC00346319] and published by . This book was released on 1984 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Recommended Specifications for Microbiological Reagents written by National Communicable Disease Center (U.S.) and published by . This book was released on 1968 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical and Biological Synthesis written by Nick J Westwood and published by Royal Society of Chemistry. This book was released on 2018-08-16 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic chemistry plays a central role in many areas of chemical biology; utilising recent case studies, the goal of Chemical and Biological Synthesis is to highlight the full impact that the preparation of novel reagents can have in chemical biology. Covering the synthetic approaches that can be applied across the whole field of chemical biology, this book provides synthetic chemists with the broader context to which their work contributes and the biological questions that can be addressed through it. An ideal guide for postgraduate students and researchers in synthetic organic chemistry and chemical biology, Chemical and Biological Synthesis introduces synthetic techniques and methods to those who wish to incorporate synthesis for the first time in their biology-focused research programmes.
Download or read book Lab Ref written by Jane Roskams and published by CSHL Press. This book was released on 2002 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Table of contents: Section 1 Most Commonly Used Solutions A. Stock Solutions, 1 B. Biological Buffers, 13 C. Proteins, Enzymes, and Antibiotics, 27 D. Reagents for the Analysis, Labeling, and Synthesis of Nucleic Acids, 35 Section 2 Macromolecular Preparation and Purification Reagents A. DNA, 43 B. RNA, 47 C. Protein, 53 Section 3 Electrophoretic Separation of Proteins and Nucleic Acids A. Electrophoresis of DNA, RNA, and Protein, 63 B. Transfer, Hybridization, and Screening of DNA, RNA, and Protein, 81 Section 4 Visualizing Genes and Gene Products A. Use of Antibodies for Immunochemical Approaches: A Guide, 95 B. Fixatives, 101 C. Cytological Stains, Chromogen Substrates, and Fluorophores, 105 D. Mounting Media, 119 E. Microscopy Information, 123 Section 5 Specialized Media, Buffers, and Reagents A. Most Commonly Used Bacterial Media and Solutions, 133 B. Yeast, 139 C. Xenopus, 155 D. Mammalian Cell Culture, 161 Section 6 Storage and Shipment of Biological Samples, 169.
Download or read book Dictionary of Analytical Reagents written by A. Townshend and published by CRC Press. This book was released on 1993-06-17 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume dictionary brings together accurate chemical, structural and bibliographic data on the most commonly used reagents in the various branches of analytical chemistry. Covering both organic and inorganic compounds, the "Dictionary of Analytical Reagents" contains over 5,000 reagents significant in analytical chemistry, grouped into 5,000 entries. All the reagents included in the dictionary have been synthesized, characterized by or are of proven use to analytical chemists. Compiled by a distinguished board of leading figures in the world of analytical chemistry, each an expert in their own specialist field, the "Dictionary of Analytical Reagents" is a companion volume to the renowned "Dictionary of Organic Compounds" and follows a similar format. The dictionary is arranged in such a way as to facilitate browsing, with entries ordered alphabetically by entry name (often its trivial name). Clearly laid out in an easy-to-follow manner, each entry contains a wealth of data invaluable to the analytical chemist including synonyms, analytical applications, extensive and up-to-date hazard/toxicity data, solubility, dissociation constant and selected references labelled to indicate their content (e.g. analytical application, spectral data, synthesis). High quality structure diagrams are included to assist the analytical chemist in identifying the reagent needed and are drawn to standard orientations. Coverage extends to metal extractants, spectrophotometric reagents, indicators, fluorescence labelling reagents, resolving agents, nmr shift reagents and reference standards, buffers, gc and ms derivatisation reagents, amperometric reagents, titrimetric and gravimetric reagents, biological stains and dyes. Compounds are comprehensively indexed by Name, Molecular Formula, CAS Registry Number and Type of Compound. The unique Type of Compound Index is particularly valuable as compounds are indexed by use (eg NMR shift reagent), by analyte (eg nickel) and by compound group (eg formazan, crown ether), making the data accessible by a variety of criteria. Thus, chemists can use the dictionary to find information on how to analyze for a particular substance, how a particular compound may be used as an analytical reagent or what other reagents are available for a specific analytical use. Having located all appropriate reagents via the index, the user can then browse through the entries to obtain specific data, all fully referenced in the selective bibliography. Analytical chemists - be they in the manufacturing or pharmaceutical industry, working in hospital laboratories as clinical chemists or pollution analysts monitoring heavy metal residues in waste water - constantly need to make decisions about which reagent to choose for a particular application. This dictionary fulfils that need by being the most comprehensive, reliable and up-to-date compilation of reagents available. This book should be of interest to analytical chemists in academic and industrial establishments, forensic scientists, chromatographers, biochemists, standards institutions, companies selling laboratory chemicals, and water authorities.
Download or read book WHO Expert Committee on Biological Standardization written by World Health Organization and published by World Health Organization. This book was released on 2018-07-18 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report presents the recommendations of a WHO Expert Committee commissioned to coordinate activities leading to the adoption of international recommendations for the production and control of vaccines and other biological substances and the establishment of international biological reference materials. Following a brief introduction the report summarizes a number of general issues brought to the attention of the Committee. The next part of the report of particular relevance to manufacturers and national regulatory authorities outlines the discussions held on the development and adoption of new and revised WHO Recommendations Guidelines and guidance documents. Following these discussions WHO Guidelines on the quality safety and efficacy of Ebola vaccines and WHO Guidelines on procedures and data requirements for changes to approved biotherapeutic products were adopted on the recommendation of the Committee. In addition the following two WHO guidance documents on the WHO prequalification of in vitro diagnostic medical devices were also adopted: (a) Technical Specifications Series (TSS) for WHO Prequalification - Diagnostic Assessment: Human immunodeficiency virus (HIV) rapid diagnostic tests for professional use and/or self-testing; and (b) Technical Guidance Series (TGS) for WHO Prequalification - Diagnostic Assessment: Establishing stability of in vitro diagnostic medical devices. Subsequent sections of the report provide information on the current status proposed development and establishment of international reference materials in the areas of: antibiotics biotherapeutics other than blood products; blood products and related substances; in vitro diagnostics; and vaccines and related substances. A series of annexes are then presented which include an updated list of all WHO Recommendations Guidelines and other documents on biological substances used in medicine (Annex 1). The above four WHO documents adopted on the advice of the Committee are then published as part of this report (Annexes 2-5). Finally all additions and discontinuations made during the 2017 meeting to the list of International Standards Reference Reagents and Reference Panels for biological substances maintained by WHO are summarized in Annex 6. The updated full catalogue of WHO International Reference Preparations is available at: http://www.who.int/bloodproducts/catalogue/en/.
Download or read book Microbial Reagents in Organic Synthesis written by S. Servi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the NATO Advanced Research Workshop, Sestri Levante, Italy, March 23-27, 1992
Download or read book Biological Detectors written by Peter A. Emanuel and published by . This book was released on 2007 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide for selection of detection devices and systems.
Download or read book Biological Substances written by and published by . This book was released on 1990 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
