Download or read book Biochemical and Immuno Histochemical Analysis of APP Processing and Amyloid Pathology in Single and Multiple Transgenic Mice as Models for Alzheimer s Disease written by Tom V. Dooren and published by Leuven University Press. This book was released on 2006-09 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transgenic Analysis of the Alzheimer s Disease Amyloid Precursor Protein APP microform written by Joannis Sekoulidis and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 2004 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Alzheimer's Disease (AD), the Amyloid Precursor Protein (APP) is endoproteolytically cleaved by beta-secretase to liberate beta-stub and subsequently processed by beta-secretase to produce Amyloid-beta (AP). Considering these endoproteolytic products have been implicated in AD pathogenesis, we have modified APP such that the cytoplasmic domain is absent and unable to support full-length beta-stub synthesis, yet able to produce full-length Abeta. By engineering mice with this transgene, we can assess whether Abeta or beta-stub cause cognitive deficits as compared to TgCRND8 mice that support synthesis of full length APP, beta-stub and Abeta. Moreover, transgenes with an altered APP copper binding domain (CuBD) have been made to prevent the post-natal lethality seen in TgCRND8 mice, while still exhibiting AD pathology. Through genetic, biochemical, and behavioural analyses of our transgenic mouse models, we will be able to define the contributions of the cytoplasmic tail and the CuBD of APP in AD pathogenesis.
Download or read book Cardiac Contractility Ca2 Handling in Mouse Models of Type II Diabetes written by An Van Den Bergh and published by Leuven University Press. This book was released on 2006-12 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Vitro and in Vivo Analysis of the Role of PEX 19 P Farnesylation and PEX 14P Phosphorylation in Peroxisome Biogenesis written by Ilse Vastiau and published by Leuven University Press. This book was released on 2006-12 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Expression and Function of a Serotoninergic System in the Rat Anterior Pituitary written by Anna-Pia Papageorgiou and published by Leuven University Press. This book was released on 2006-12 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrical Brain Stimulation in Psychiatric Disorders Contributions from Research in Animal Models written by Kris Van Kuyck and published by Leuven University Press. This book was released on 2006 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effects of Il 6 Il 8 on Respiratory Peripheral Skeletal Muscle Function written by Sofie Janssen and published by Leuven University Press. This book was released on 2006-12-30 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Axon Growth Injury and Regeneration in the Fly Brain written by Maarten Leyssen and published by Leuven University Press. This book was released on 2006 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nitric Oxide and Cytochrome P450 Signaling in Experimental Pulmonary Hypertension written by Peter Pokreisz and published by Leuven University Press. This book was released on 2006 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantification of Regional Myocardial Deformation Normal Characteristics and Clinical Use in Ischaemic Heart Disease written by Lieven Herbots and published by Leuven University Press. This book was released on 2006 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Experimental Evaluation of the Host Immune Response to Xenogenic Bio derived Collagen Grafts in Rodent Models written by Fang Zheng and published by Leuven University Press. This book was released on 2006-09 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Repopulation of Bioprosthetic Heart Valves written by Jessa Yperman and published by Leuven University Press. This book was released on 2006 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of Amyloid Precursor Protein in Cells and Brain of Transgenic Mice As the Biochemical Correlation for Their Phenotype written by Ilse Dewachter and published by . This book was released on 1999-12-01 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on Early CNS Inflammation in Transgenic Models of the Alzheimer s Pathology written by Luisa Sa Barreto Pimentel and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Many advances have being made in understanding the pathological processes in Alzheimer's Disease (AD) and in testing therapeutic strategies to stop disease progression. It has become clear that neuroinflammation plays a main role in AD. Evidence is being gathered from experimental, epidemiological and clinical trials studies that this process happens even earlier, in stages where pathology is present but no symptoms are detected. Even though the late, plaque-associated central nervous system (CNS) inflammation is well characterized, many questions remain regarding the early inflammatory process. Understanding this event is important to develop biomarkers to identify those at risk for AD and to monitor disease progression, and in the search of better treatment for AD. Unfortunately, studying pre-clinical stages in AD is not possible as yet due to the lack of biomarkers signalling the conversion from normal cognition to AD. Alternatively early stages of the AD-like amyloid pathology can be study in transgenic (tg) models that mimic several aspects of the human pathology. In the studies described here we use both our AD tg rat (McGill-R-Thy1-APP) and mouse (McGill-Thy1-APP) models to investigate events on the early amyloid pathology. We first demonstrated a marked up-regulation of several classical inflammatory markers such as COX-2, IL-1[beta], TNF-[alpha] and fractalkine (CX3CL1) in the cerebral cortex and hippocampus. Interestingly, many of these markers were highly expressed in A[beta]-burdened neurons. Activated astrocytes and microglia were found in close relationship to these A[beta]-burdened neurons. These findings confirm the occurrence of a pro-inflammatory process preceeding the generation of amyloid plaques and suggest that A[beta]-burdened neurons play a crucial and early role in the initial inflammation in Alzheimer's pathology.Further we investigated the impact systemic inflammation in these very early stages of both amyloid pathology and CNS inflammation, as well as its impact on cognition. We found that administration of lipopolysaccharide (LPS) to McGill-Thy1-APP tg mice - at a pre-plaque stage of the amyloid pathology - changed levels of both anti (IL-10 and TGF-[beta]) and pro-inflammatory molecules (COX-2 and MCP-1) as well the status of the microglia, a important cell mediator of inflammation. On the other hand LPS-administration did not impact cortical human A[beta]42 levels, the profile of astrocytosis in the cortex and hippocampus of these mice or their cognitive status. Here we showed a chronic and long-lasting effect of LPS-induced systemic inflammation in CNS inflammation in initial stages of the amyloid pathology which is not present in mice with no amyloid pathology. Taken together these data indicates that systemic inflammation could have a negative impact on these initial/asymptomatic stages of AD pathology. Finally we study the impact of M30, a drug with antioxidant and neuroprotective properties, regarding its potential anti-inflammatory effects and its beneficial effects on cognition in our tg rat model of AD. We found that M30 administration reverted deficits in Novel Objection Location task and prevented worsening of cognitive impairment in Novel Object Recognition paradigm. Furthermore we demonstrated that the treatment rescued microglia phenotype, decreasing activation of these cells which is associated with the release of neurotoxic molecules. This change is microglia phenotype correlates with the effects on cognition in which activated microglia is associated with poor cognitive performance. Taken together, our results suggest the presence of neuronal-driven CNS inflammatory process associated with the accumulation of oligomeric A[beta]. This early pathological event is modulated by systemic inflammation which might aggravate this process. By the other hand intervention with multi-target M30 decreases the inflammatory response with beneficial impacts on cognition. " --
Download or read book Amyloid Proteins written by Einar M. Sigurdsson and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book The Biology of Alzheimer Disease written by Dennis J. Selkoe and published by Cold Spring Harbor Perspective. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alzheimer disease causes the gradual deterioration of cognitive function, including severe memory loss and impairments in abstraction and reasoning. Understanding the complex changes that occur in the brain as the disease progressesincluding the accumulation of amyloid plaques and neurofibrillary tanglesis critical for the development of successful therapeutic approaches. Written and edited by leading experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine includes contributions covering all aspects of Alzheimer disease, from our current molecular understanding to therapeutic agents that could be used to treat and, ultimately, prevent it. Contributors discuss the biochemistry and cell biology of amyloid -protein precursor (APP), tau, presenilin, -secretase, and apolipoprotein E and their involvement in Alzheimer disease. They also review the clinical, neuropathological, imaging, and biomarker phenotypes of the disease; genetic alterations associated with the disorder; and epidemiological insights into its causation and pathogenesis. This comprehensive volume, which includes discussions of therapeutic strategies that are currently used or under development, is a vital reference for neurobiologists, cell biologists, pathologists, and other scientists pursuing the biological basis of Alzheimer disease, as well as investigators, clinicians, and students interested in its pathogenesis, treatment, and prevention.
Download or read book Molecular Pathology of Alzheimer s Disease written by Rudy Castellani and published by Biota Publishing. This book was released on 2013-10-01 with total page 93 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alzheimer’s Disease is characterized pathologically by two principal hallmark lesions: the senile plaque and the neurofibrillary tangle. Since the identification of each over 100 years ago, the major protein components have been elucidated. This has led in turn to the elaboration of metabolic cascades involving amyloid-β production in the case of the senile plaque, and phosphorylated-tau protein in the case of the neurofibrillary tangle. The pathogenesis and histogenesis of each have been the source of extensive investigation and some controversy in recent years, as both cascades have been implicated in the pathogenesis of Alzheimer’s Disease, relied upon in the diagnostic criteria for Alzheimer’s Disease at autopsy, and targeted for therapeutic intervention. With the accumulation of data and expansion of knowledge of the molecular biology of Alzheimer’s Disease, it appears that the enthusiasm for successful intervention has been premature. In this book, we detail the discovery and characterization of the major pathological lesions, their associated molecular biology, their relationship to clinical disease, and potential fundamental errors in understanding that may be leading scientific investigators in unintended directions.
