Download or read book Biocatalytic Synthesis of Pharmaceutically Relevant Amides and Amino Acids written by and published by . This book was released on 2021 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biocatalysis for the Pharmaceutical Industry written by Junhua (Alex) Tao and published by John Wiley & Sons. This book was released on 2009-07-23 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalysis is rapidly evolving into a key technology for the discovery and production of chemicals, especially in the pharmaceutical industry, where high yielding chemo-, regio-, and enantioselective reactions are critical. Taking the latest breakthroughs in genomics and proteomics into consideration, Biocatalysis for the Pharmaceutical Industry concisely yet comprehensively discusses the modern application of biocatalysis to drug discovery, development, and manufacturing. Written by a team of leading experts, the book offers deep insight into this cutting edge field. Covers a wide range of topics in a systematic manner with an emphasis on industrial applications Provides a thorough introduction to the latest biocatalysts, modern expression hosts, state-of-the-art directed evolution, high throughput screening, and bioprocess engineering Addresses frontier subjects such as emerging enzymes, metabolite profiling, combinatorial biosynthesis, metabolic engineering, and autonomous enzymes for the synthesis and development of chiral molecules, drug metabolites, and semi-synthetic medicinal compounds and natural product analogs Highlights the impact of biocatalysis on green chemistry Contains numerous graphics to illustrate concepts and techniques Biocatalysis for the Pharmaceutical Industry is an essential resource for scientists, engineers, and R&D policy makers in the fine chemical, pharmaceutical, and biotech industries. It is also an invaluable tool for academic researchers and advanced students of organic and materials synthesis, chemical biology, and medicinal chemistry.
Download or read book Biocatalytic Preparation of Amino Acids written by Vivek Kothari and published by Daya Books. This book was released on 2007 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amino Acids Are The Molecular Units That Make Up Proteins. All Proteins Are Various Compositions Of Twenty Specific Naturally Occurring Amino Acids. Amino Acids Are Found In All Foods Except Oil. There Are About Eighty Amino Acids Found In Nature, But Only Twenty To Twenty-Nine Are Required For Human Growth. Amino Acids Play A Pivotal Role In Living Systems, And Therefore Synthetic Amino Acids Have Been Of Significant Interest To Researchers Working Towards The Understanding And Modification Of Physiological Processes. Currently, The Amino Acids Used In Amino Acid Products Are Mainly Manufactured By Biocatalytic Methods. The Book Presents Detailed Discussion Of The Preparation Of Amino Acids By Biocatalysis. It Provides Examples Of Recently Developed Biocatalytic Methods For The Production Of Amino Acids And Its Derivatives. It Offers Upper Level Students And Professionals An Essential Resource For Pharmaceutical Development, Medicinal Chemistry And Biochemistry. Contents Chapter 1: Basics Of Biocatalysis; Chapter 2: Production Of Amino Acids; Chapter 3: Asymmetric Synthesis Of Amino Acids; Chapter 4: Amino Acids And Derivatives; Chapter 5: Strategies For Efficient Biocatalysis; Chapter 6: Synthesis Of Cage Amino Acids; Chapter 7: Types Of B-Amino Acids; Chapter 8: Extremophiles And Extremozymes; Chapter 9: Immobilised Enzymes; Chapter 10: Enzymatic Peptide Synthesis; Chapter 11: Synthesis Of Enantiomerically Pure (S)-Amino Acids And (R)-Amines; Chapter 12: Cloning And Sequence Analysis; Chapter 13: Bioseparation And Biocatalytic Applications.
Download or read book Cascade Biocatalysis written by Sergio Riva and published by John Wiley & Sons. This book was released on 2014-06-23 with total page 587 pages. Available in PDF, EPUB and Kindle. Book excerpt: This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed. From the contents: * Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond * New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors * Monooxygenase-Catalyzed Redox Cascade Biotransformations * Biocatalytic Redox Cascades Involving w-Transaminases * Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases * Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates * Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids * Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution * Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode * Perspectives on Multienzyme Process Technology * Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions * Mining Genomes for Nitrilases * Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion * Enzymatic Stereoselective Synthesis of Beta-Amino Acids * New Applications of Transketolase: Cascade Reactions for Assay Development * Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs * Enzymatic Generation of Sialoconjugate Diversity * Methyltransferases in Biocatalysis * Chemoenzymatic Multistep One-Pot Processes
Download or read book Biocatalysis in the Pharmaceutical and Biotechnology Industries written by Ramesh N. Patel and published by CRC Press. This book was released on 2006-09-26 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because enzyme-catalyzed reactions exhibit higher enantioselectivity, regioselectivity, substrate specificity, and stability, they require mild conditions to react while prompting higher reaction efficiency and product yields. Biocatalysis in the Pharmaceutical and Biotechnology Industries examines the use of catalysts to produce fine chemic
Download or read book Catalytic and Biocatalytic Methods for the Efficient Synthesis of Biologically Relevant Non Proteinogenic Amino Acids written by Anke Lemke and published by Cuvillier Verlag. This book was released on 2014-04-29 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-proteinogenic amino acids are often important constituents of biologically potent natural products. The synthesis of these amino acids is often time-consuming, non-trivial and not sustainable. Thus, the central topic of this work was the formation of unusual amino acid structures by biocatalytic and catalytic methods. For the elucidation of the biosynthetic pathway of Streptomyces-produced muraymycins and related natural products, a ‘biosynthetic tool kit’ was compiled and prepared. This does not only comprehend the completed synthesis of deuterium-labeled compounds, such as (3R)- and (3S)-3-hydroxy-[5-2H]-l-arginine and other potential intermediates with a nucleosidic structure, but it also includes the development of fermentation methodology including the possibility to detect the muraymycin derivatives by LC MS. Furthermore, different synthetic routes with catalytic key steps for the preparation of the non-proteinogenic amino acid enduracididine were investigated.
Download or read book Practical Methods for Biocatalysis and Biotransformations 2 written by John Whittall and published by John Wiley & Sons. This book was released on 2012-04-25 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a "how-to" guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume – which can be used on its own or in combination with the first volume - concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.
Download or read book Biocatalytic Synthesis of gamma hydroxy Amino Acids and Computational Exploration of Sequence activity Landscapes written by Jonathan Michael Ellis and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Through the course of evolution, Nature has developed a swath of useful enzymes for catalyzing chemical reactions crucial to life. These enzymes are used in complex metabolic cascades to produce a wide variety of useful natural products in a chemo-, regio-, and stereoselective fashion. These enzymes can be adopted in synthetic chemistry contexts to afford powerful tools for synthesizing complex molecules in a green way. Through protein engineering, it is possible to further expand the applicability of these enzymes, and by using computational modelling tools, it is possible to accelerate the engineering process. Here, I discuss my efforts in engineering two useful enzymes pyridoxal 5'-phosphate-dependent enzymes, UstD and RgnTDC, to expand their substrate scopes and applicability. UstD is capable of directly installing an amino acid moiety into electrophilic aldehyde substrates through a decarboxylative aldol-like reaction from L-aspartic acid. Through multiple rounds of directed evolution, including linear regression guided evolution, we were able to improve the activity and expressibility of UstD. Through mechanistic studies and crystallography, we have begun probing the complex catalytic cycle that enables UstD to perform its catalyzed reaction, as well as laying the foundation for structure guided evolution. Complementary to UstD, RgnTDC is a l-tryptophan decarboxylase which is able to decarboxylate amino acids and generate precursors to medicinally relevant compounds. Through the use of Substrate Multiplexed Screening (SUMS) in conjunction with logistic regression guided directed evolution and primer-doped mutagenesis, we have created a variety of generalist biocatalysts with diverse active site composition. These variants serve as potential starting points for future engineering campaigns. To help facilitate these future engineering efforts and explore the utility of SUMS, I have also worked to develop computational tools to process and analyze SUMS library screening data. These tools have been integrated into a single web-based program, titled the SUMS Interactive Processing Software (SIPS), and have been developed with broad applicability and community use in mind.
Download or read book Biodiversity Ecosystem Functioning and Human Wellbeing written by Shahid Naeem and published by Oxford University Press. This book was released on 2009-07-30 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book starts by summarizing the development of the basic science and provides a meta-analysis that quantitatively tests several biodiversity and ecosystem functioning hypotheses.
Download or read book Biocatalysis written by D.A. Abramowicz and published by Springer. This book was released on 1990-06-30 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: The action of enzymes fascinated mankind long before they were rec ognized for the complex chemicals that they are. The first application of these remarkable compounds to produce ethanol by fermentation is lost to antiquity. Payer and Persoz (Ann. Chim. Phys. , 53, 73 (1833ii)) appear to have provided the first step toward understanding this com plex area when they reported the isolation of diastase in 1833. These workers showed that diastase could catalyze the hydrolysis of starches to sugars. Somewhat earlier Kirchhoff (Schwigger's Journal, 4, 108 (1812)) had shown that a small amount of dilute acid could hydrolyze a seemingly endless amount of starch to sugars. The genius of Berzelius recognized the commonality of these two observations in connection with a few other isolated observations and in 1834 coined the term catalysis to describe such actions. Professor Leibig was one of the giants of the chemical world in 1840. In addition to his own work, Liebig was training the world's next generation of chemists in his laboratory in Giessen. This cadre of chemists were very impressed by the master teacher so that is it only natural that Liebig's views should dominate with this next generation of chemists. Leibig was, in the 1830s and 1840s, developing his mastery of agricultural chemistry. The mechanism of putrefication was of great concern to Leibig, and he turned to the newly defined area of catalysis for an explanation.
Download or read book Applied Biocatalysis written by John Whittall and published by John Wiley & Sons. This book was released on 2020-08-21 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistry This book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and includes short but detailed experimental methods on timely novel biocatalytic transformations using new enzymes or new methodologies using known enzymes. The book also features reactions that are “expanding and making the enzyme toolbox available to chemists”—providing readers with comprehensive methodology and detailed key sourcing information of a wide range of enzymes. Chapters in Applied Biocatalysis: The Chemist’s Enzyme Toolkit are organized by reaction type and feature a short introductory section describing the current state of the art for each example. Much of the book focuses on processes for which the enzymes are readily available so that organic chemists can synthesize appropriate quantities of chemicals with available materials in a standard chemical laboratory. Advanced methods are included to present examples of new enzymes that might encourage collaboration with suppliers or academic groups and that will educate chemists of rapidly expanding future possibilities. Focuses on current topics within the applied industrial biocatalysis field Offers experimental methods on novel biocatalytic transformations using new enzymes or new methodology using known enzymes Covers the hot topics of enzyme and chemoenzymatic cascades and biocatalysis in flow Edited by noted experts from both academia and industry with years of experience in the field of biocatalysis—particularly, the industrial applications of enzymes Written for synthetic organic chemists working in all industries but especially the pharmaceutical industry and for those in academia with an eye for biocatalysis, Applied Biocatalysis: The Chemist’s Enzyme Toolkit will also benefit academic groups in chemistry and related sciences that are using enzymes for synthetic purposes, as well as those working in the area of enzymology and molecular biology.
Download or read book Modification of Polymers written by Charles E. Carraher and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: The sheer volume of topics which could have been included under our general title prompted us to make some rather arbitrary decisions about content. Modification by irradiation is not included because the activity in this area is being treated elsewhere. We have chosen to emphasize chemical routes to modification and have striven to pre sent as balanced a representation of current activity as time and page count permit. Industrial applications, both real and potential, are included. Where appropriate, we have encouraged the contributors to include review material to help provide the reader with adequate context. The initial chapter is a review from a historical perspective of polymer modification and contains an extensive bibliography. The remainder of the book is divided into four general areas: Reactions and Preparation of Copolymers Reactions and Preparation of Block and Graft Copolymers Modification Through Condensation Reactions Applications The chemical modification of homopolymers such as polyvinylchlo ride, polyethylene, poly(chloroalkylene sulfides), polysulfones, poly chloromethylstyrene, polyisobutylene, polysodium acrylate, polyvinyl alcohol, polyvinyl chloroformate, sulfonated polystyrene; block and graft copolymers such as poly(styrene-block-ethylene-co-butylene block-styrene), poly(I,4-polybutadiene-block ethylene oxide), star chlorine-telechelic polyisobutylene, poly(isobutylene-co-2,3-dimethyl- 1,3-butadiene), poly(styrene-co-N-butylmethacrylate); cellulose, dex tran and inulin, is described.
Download or read book Engineering Aminotransferases for the Biocatalytic Production of Aromatic D Amino Acids written by Curtis James William Walton and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Optically pure aromatic D-amino acids, such as D-phenylalanine (D-Phe) and its derivatives, are high-value building blocks for the pharmaceutical industry. These compounds can be prepared using biocatalytic methods relying on various enzymes, including aminotransferases (ATs). ATs, also called transaminases (EC 2.6.1.X), are a subclass of pyridoxal 5'-phosphate-dependent enzymes that catalyze the transfer of the amino group from a donor substrate to a ketone acceptor. Synthesis of optically-pure amino acids using whole-cell biocatalytic cascades based on ATs possess several advantages compared to traditional chemical methods, including excellent enantioselectivity and increased process and step efficiency, which is achieved through the catalysis of multiple steps in one-pot reactions without requirement for intermediate work-ups, cofactor recycling, or toxic metals. However, enzyme biocatalysts typically need to be engineered to alter their substrate specificity or to increase their catalytic efficiency, which has limited their industrial application. Therefore, to facilitate the engineering process of ATs broadly and to produce aromatic D-amino acids, we developed a high-throughput assay for the testing of a broad range of ATs against libraries of potential substrates, and developed a biocatalytic cascade to produce optically pure aromatic D-amino acids.
Download or read book Biocatalysis in Organic Synthesis written by Nicholas J. Turner and published by Royal Society of Chemistry. This book was released on 2018-02-08 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of biocatalysis in organic synthesis is rapidly gaining popularity amongst chemists. Compared to traditional synthetic methodologies biocatalysis offers a number of advantages in terms of enhanced selectivity (chemo-, regio-, stereo-), reduced environmental impact and lower cost of starting materials. Together these advantages can contribute to more sustainable manufacturing processes across a wide range of industries ranging from pharmaceuticals to biofuels. The biocatalytic toolbox has expanded significantly in the past five years and given the current rate of development of new engineered biocatalysts it is likely that the number of available biocatalysts will double in the next few years. This textbook gives a comprehensive overview of the current biocatalytic toolbox and also establishes new guidelines or rules for “biocatalytic retrosynthesis”. Retrosynthesis is a well known and commonly used technique whereby organic chemists start with the structure of their target molecule and generate potential starting materials and intermediates through a series of retrosynthetic disconnections. These disconnections are then used to devise a forward synthesis, in this case using biocatalytic transformations in some of the key steps. Target molecules are disconnected with consideration for applying biocatalysts, as well as chemical reagents and chemocatalysts, in the forward synthesis direction. Using this textbook, students will be able to place biocatalysis within the context of other synthetic transformations that they have learned earlier in their studies. This additional awareness of biocatalysis will equip students for the modern world of organic synthesis where biocatalysts play an increasingly important role. In addition to guidelines for identifying where biocatalysts can be applied in organic synthesis, this textbook also provides examples of current applications of biocatalysis using worked examples and case studies. Tutorials enable the reader to practice disconnecting target molecules to find the ‘hidden’ biocatalytic reactions which can be applied in the synthetic direction. The book contains a complete description of the current biocatalyst classes that are available for use and also suggests areas where new enzymes are likely to be developed in the next few years. This textbook is an essential resource for lecturers and students studying synthetic organic chemistry. It also serves as a handy reference for practicing chemists who wish to embed biocatalysis into their synthetic toolbox.
Download or read book Biocatalytic Synthesis of Amino Alcohols written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Industrial Biocatalysis written by Peter Grunwald and published by CRC Press. This book was released on 2014-12-11 with total page 1201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalysis has become an essential tool in the chemical industry and is the core of industrial biotechnology, also known as white biotechnology, making use of biocatalysts in terms of enzymes or whole cells in chemical processes as an alternative to chemical catalysts. This shift can be seen in the many areas of daily life where biocatalysts-with
Download or read book Biotransformations in Organic Chemistry written by Kurt Faber and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of natural catalysts - enzymes - for the transformation of non-natural is not at all new: they have been used for more man-made organic compounds than one hundred years, employed either as whole cells, cell organelles or isolated enzymes [1]. Certainly, the object of most of the early research was totally different from that of the present day. Thus the elucidation of biochemical pathways and enzyme mechanisms was in the foreground of the reasearch some decades ago. It was mainly during the 1980s that the enormous potential of applying natural catalysts to transform non-natural organic compounds was recognized. What started as a trend in the late 1970s could almost be called a fashion in synthetic organic chemistry in the 1990s. Although the early euphoria during the 'gold rush' in this field seems to have eased somewhat, there is still no limit to be seen for the future development of such methods. As a result of this extensive, recent research, there have been an estimated 5000 papers published on the subject [2]. To collate these data as a kind of 'super-review' would clearly be an impossible task and, furthermore, such a hypothetical book would be unpalatable for the non-expert.
