Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Basic Methods in Molecular Biology written by Leonard G. Davis and published by . This book was released on 1992 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another.
Download or read book Molecular Biology Techniques written by Walt Ream and published by Academic Press. This book was released on 1998-11-17 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter
Download or read book Techniques in Molecular Biology written by J.M. Walker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Download or read book Basic Methods in Molecular Biology written by Leonard G. Davis and published by . This book was released on 1986 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology-the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; an ...
Download or read book Advanced Methods in Molecular Biology and Biotechnology written by Khalid Z. Masoodi and published by Academic Press. This book was released on 2020-10-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Download or read book Techniques in Molecular Medicine written by Friedhelm Hildebrandt and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Download or read book Basic Techniques In Molecular Biology written by Surzycki and published by . This book was released on 2007-10-01 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Basic Methods in Molecular Biology written by Leonard Davis and published by Elsevier. This book was released on 2012-12-02 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.
Download or read book Basic Molecular Biology written by Philippa D. Darbre and published by Wiley-Blackwell. This book was released on 1999-01-12 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the increasing number of professionals needing to learn about and use molecular biology techniques in their research, the daunting challenge is to break through the technical and largely inaccessible jargon found in most texts. This unique introduction to the field makes things much easier. The author begins with a brief overview of the basics, then focuses on simple step-by-step protocols of the core techniques, giving a theoretical outline, valuable advice on real-world problems readers might encounter, and estimates on how much time is required to perform each technique.
Download or read book Basic Techniques in Biochemistry Microbiology and Molecular Biology written by Aakanchha Jain and published by Humana. This book was released on 2021-03-14 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.
Download or read book Techniques for Molecular Biology written by D Tagu and published by CRC Press. This book was released on 2006-01-04 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explains the theoretical principles of numerous techniques of genomic studies developed recently in laboratories. The book is designed for any reader who is curious to know the bases of different techniques of manipulation of nucleic acids.
Download or read book Practical Methods in Molecular Biology written by Robert F. Schleif and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.
Download or read book Methods in Plant Molecular Biology written by Mary A. Schuler and published by Academic Press. This book was released on 2012-12-02 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. Eight basic experiments which can be used collectively or individually cover Recombinant Cloning and Screening in E. coli; DNA Sequencing Plant RNA Isolation and in Vitro Translations Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations Experiments that have been student tested for three years Blueprints for setting up gel rigs Comprehensive course schedule outlining individual procedures to be finished in each lab segment Course can be tailored to suit the needs of the individual instructor
Download or read book Plant Molecular Biology written by P. G. Jones and published by . This book was released on 1997-10-09 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors describe the most important currently used protocols in plant molecular biology. They give useful tips on stopping points and troubleshooting as well as providing information on safety. Lists of suppliers are included.
