Download or read book Basic Protein and Peptide Protocols written by John M. Walker and published by Humana. This book was released on 1994-04-26 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Download or read book Methods in Molecular Biology Basic protein and peptide protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 1996 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: -- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students
Download or read book Protein Purification Protocols written by Shawn Doonan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.
Download or read book Modeling Peptide Protein Interactions written by Ora Schueler-Furman and published by Humana. This book was released on 2017-02-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers an array of techniques available for studying peptide-protein docking and design. The book is divided into four sections: peptide binding site prediction; peptide-protein docking; prediction and design of peptide binding specificity; and the design of inhibitory peptides. The chapters in Modeling Peptide-Protein Interactions: Methods and Protocols cover topics such as the usage of ACCLUSTER and PeptiMap for peptide binding site prediction; AnchorDock and ATTRACT for blind, flexible docking of peptides to proteins; flexible peptide docking using HADDOCK and FlexPepDock; identifying loop-mediated protein-protein interactions using LoopFinder; and protein-peptide interaction design using PinaColada. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary details for successful application of the different approaches and step-by-step, readily reproducible protocols, as well as tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Peptide-Protein Interactions: Methods and Protocols provides a diverse and unified overview of this rapidly advancing field of major interest and applicability.
Download or read book Modeling Peptide Protein Interactions Methods and Protocols written by Ora Schueler-Furman and published by Methods in Molecular Biology. This book was released on 2019-03-13 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers an array of techniques available for studying peptide-protein docking and design. The book is divided into four sections: peptide binding site prediction; peptide-protein docking; prediction and design of peptide binding specificity; and the design of inhibitory peptides. The chapters in Modeling Peptide-Protein Interactions: Methods and Protocols cover topics such as the usage of ACCLUSTER and PeptiMap for peptide binding site prediction; AnchorDock and ATTRACT for blind, flexible docking of peptides to proteins; flexible peptide docking using HADDOCK and FlexPepDock; identifying loop-mediated protein-protein interactions using LoopFinder; and protein-peptide interaction design using PinaColada. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary details for successful application of the different approaches and step-by-step, readily reproducible protocols, as well as tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Peptide-Protein Interactions: Methods and Protocols provides a diverse and unified overview of this rapidly advancing field of major interest and applicability.
Download or read book Short Protocols in Protein Science written by John E. Coligan and published by Wiley-Blackwell. This book was released on 2003-10-24 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: Short Protocols in Protein Science provides condensed descriptions of more than 500 protocols compiled from Current Protocols in Protein Science. Drawing from both the original "core" manual as well as the quarterly update service, this compendium includes all step-by-step descriptions of the principal methods covered in Current Protocols in Protein Science.
Download or read book Peptide Characterization and Application Protocols written by Gregg B. Fields and published by Humana Press. This book was released on 2010-11-19 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.
Download or read book Current Protocols in Protein Science written by John E. Coligan and published by Current Protocols. This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists across disciplines have increasingly come to recognize the power of the protein. Current Protocols in Protein Science, a two-volume looseleaf manual, was developed in response to this revitalized interest and provides the most comprehensive collection of expert protein methods available. The publication covers both basic and advanced methods used in protein purification, characterization, and analysis as well as post-translational modification and structural analysis. More than 800 basic, support and alternate protocols have been carefully chosen for maximum applicability. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly updates, which are filed into the looseleaf, keep the set current with the latest developments in protein science methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.
Download or read book Mass Spectrometry of Proteins and Peptides written by Mary S. Lipton and published by Humana Press. This book was released on 2008-12-18 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.
Download or read book Protein Sequencing Protocols written by Bryan John Smith and published by Springer Science & Business Media. This book was released on 1997 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: If the development of techniques for the labeling of a polypeptide- terminus and for repetitive N-terminal sequencing mark the beginning of the science of protein structure determination, then the field has just about reached its half century. In more recent times, recombinant DNA techniques have pro vided powerful means by which to obtain long protein sequences (by theoreti cal translation from nucleic acid sequences), but rather than replacing the direct, chemical, protein sequencing approach, they have instead added further impe tus to the drive towards a better understanding of posttranslational processing and modification events, as well as identification of novel proteins. Recent years have also seen the advent of "biopharmaceuticals" (i. e. , pharmaceutical products that are proteins, such as monoclonal antibodies), and this has meant that protein sequencing has found an important new application as a quality control tool. Over the decades of protein sequencing many new techniques have been introduced, with the basic aim of generating more information from less material. Some techniques have come and gone, but others have been with us for many years. Edman chemistry is perhaps the best example of the latter class, with its basic principles still being applied today in the newest protein sequencer design. Methods for cleaving and modifying peptides are also long standing, having been adapted over the years to suit progressively smaller amounts of sample.
Download or read book Peptide and Protein Delivery written by Chris Van Der Walle and published by Academic Press. This book was released on 2011-05-12 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growing area of peptide and protein therapeutics research is of paramount importance to medical application and advancement. A needed reference for entry level researchers and researchers working in interdisciplinary / collaborative projects, Peptide and Protein Delivery addresses the current and emerging routes for delivery of therapeutics. Covering cerebral delivery, pulmonary delivery, transdermal delivery, intestinal delivery, ocular delivery, parenteral delivery, and nasal delivery, this resource offers an overview of the main routes in therapeutics. Researchers across biochemistry, pharmaceutical, molecular biology, cell biology, immunology, chemistry and biotechnology fields will find this publication invaluable for peptide and protein laboratory research. - Discusses the most recent data, ideas and concepts - Presents case studies and an industrial perspective - Details information from the molecular level to bioprocessing - Thought provoking, for the novice to the specialist - Timely, for today's biopharmaceuticals market
Download or read book A Practical Guide to Protein and Peptide Purification for Microsequencing written by Paul T. Matsudaira and published by Elsevier. This book was released on 2012-12-02 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing
Download or read book Computational Peptide Science written by Thomas Simonson and published by Humana. This book was released on 2022-03-18 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details current and new computational methodologies to study peptides. Chapters guide readers through antimicrobial peptides, foldability, amyloid sheet formation, membrane-active peptides, organized peptide assemblies, protein-peptide interfaces, prediction of peptide-MHC complexes, advanced free energy simulations for peptide binding, and methods for high throughput peptide or miniprotein design. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials, software, and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computational Peptides Science: Methods and Protocols aims to provide concepts, methods, and guidelines to help both novices and experienced workers benefit from today's new opportunities and challenges.
Download or read book Peptide Protocols written by MD William A. Seeds and published by . This book was released on 2020-08-24 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Basic Protein and Peptide Protocols written by John M. Walker and published by Humana Press. This book was released on 1994-04-26 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Download or read book Therapeutic Proteins written by C. Mark Smales and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the recent completion of the sequencing of the human genome, it is widely anticipated that the number of potential new protein drugs and targets will escalate at an even greater rate than that observed in recent years. However, identification of a potential target is only part of the process in developing these new next generation protein-based “drugs” that are increasingly being used to treat human disease. Once a potential protein drug has been identified, the next rate-limiting step on the road to development is the production of sufficient authentic material for testing, charact- ization, clinical trials, and so on. If a protein drug does actually make it through this lengthy and costly process, methodology that allows the production of the protein on a scale large enough to meet demand must be implemented. Furthermore, large-scale production must not compromise the authenticity of the final product. It is also nec- sary to have robust methods for the purification, characterization, viral inactivation and continued testing of the authenticity of the final protein product and to be able to formulate it in a manner that retains both its biological activity and lends itself to easy administration. Therapeutic Proteins: Methods and Protocols covers all aspects of protein drug production downstream of the discovery stage. This volume contains contributions from leaders in the field of therapeutic protein expression, purification, characterization, f- mulation, and viral inactivation.
