Download or read book Bacterial Targets of Gut Mucosal Immunoglobulin A Responses in Healthy and Undernourished Children and in Gnotobiotic Mice Colonized with Human Gut Microbiota written by Joseph Planer and published by . This book was released on 2017 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: The adaptive immune response to the human gut microbiota consists of a complex repertoire of antibodies interacting with a broad range of taxa. In mammals, immunoglobulin A (IgA) is the major class of antibody secreted at mucosal surfaces, where it promotes gut barrier function by preventing microbial and food antigens from interacting with host cells/tissues. The organisms targeted by gut mucosal IgA responses, the molecular targets of these secreted antibodies, and the environmental and genetic factors that shape these responses in the gut remain poorly defined. The central hypotheses of my thesis are: (i) IgA responses to human gut bacteria help to establish and maintain the mutually beneficial relationship between members of the microbiota and the host, including the fitness and expressed features of these members, (ii) IgA-targeting of specific bacterial taxa can serve as a biomarker for barrier disruption and be used to purify bacterial consortia with disease-effecting or disease-attenuating properties, and (iii) during the course of the first two years of postnatal life gut mucosal immune responses converge on a shared pattern of IgA-targeting in healthy infants that can be modeled within and between twin pairs and in gnotobiotic mice colonized with human fecal microbiota and fed diets representative of the donor human population. The first chapter in my thesis provides a brief synopsis of our current understanding of interactions between the microbiota and gut mucosal immunity. The second chapter contrasts the functional effects of two naturally-primed monoclonal IgAs that recognize distinct surface epitopes on the model human gut symbiont, Bacteroides thetaiotaomicron. Studies in three collections of B. thetaiotaomicron isolates and in gnotobiotic mice monocolonized with B. thetaiotaomicron are used to explore strain- and species-level epitope conservation, the fitness effects of these conserved epitopes in vivo, and the effects of antibody binding on bacterial gene expression. In the third chapter, I examine interrelationships between the gut microbiota and intestinal IgA responses in children with varying degrees of undernutrition, and gut barrier function. This study used fluorescence-activated cell sorting to purify IgA-bound and unbound fractions of a fecal microbial community ('BugFACS'). We applied this technique to (i) fecal samples collected from mice harboring the fecal microbial communities of a Malawian twin pair discordant for kwashiorkor (a form of severe acute malnutrition), and fed either a sterile macro- and micronutrient deficient diet designed to represent the diets of the donor population or a nutrient sufficient diet, (ii) fecal samples collected from other twin pairs in this cohort that were discordant for severe acute malnutrition, and (iii) fecal samples collected from a second cohort of Malawian children with moderate acute malnutrition. These experiments disclosed that IgA responses to the bacterial family Enterobacteriaceae were robust biomarkers for health status. Follow-up experiments in gnotobiotic mice disclosed that a BugFACS-purified IgA+ consortium of bacteria could transmit a severe enteropathy characterized by rapid barrier disruption in the colon and small intestine, weight loss, and sepsis. Fractionation of this bacterial consortium disclosed that members of the Enterobacteriaceae were necessary but not sufficient to cause the profound weight loss and barrier disruption, while IgA-targeted members of the 'healthy' co-twin's microbiota could prevent these phenotypes. In the fourth chapter, I analyze the co-development of gut microbiota and gut mucosal IgA responses in a birth cohort of 40 healthy USA twin pairs. I model development of the gut microbiota using a Random Forests-based machine learning approach that yielded a set of 25 bacterial taxa that could describe the maturation of fecal microbial communities in unrelated children. Applying BugFACS to a subset of these fecal samples, I further characterize gut mucosal IgA responses to components of the microbiota, and show how they vary as a function of postnatal age, family, and diet. These analyses reveal that there is an identifiable pattern of progression of gut mucosal IgA responses to members of the microbiota from one that in the first several months of postnatal life is highly distinctive for family members sharing a common environment (exemplified by healthy twin pairs), to one that subsequently generalizes across families (twin pairs) during the second postnatal year. I then present data from gnotobiotic mouse experiments showing that IgA responses in these mice broadly mirrored those of the human donor population and recapitulated age-associated differences observed in the twin pairs. The fifth chapter of my thesis proposes several potential avenues for future research based on the findings in my thesis.

Download or read book The Human Microbiome Diet and Health written by Food Forum and published by National Academies Press. This book was released on 2013-02-27 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Food Forum convened a public workshop on February 22-23, 2012, to explore current and emerging knowledge of the human microbiome, its role in human health, its interaction with the diet, and the translation of new research findings into tools and products that improve the nutritional quality of the food supply. The Human Microbiome, Diet, and Health: Workshop Summary summarizes the presentations and discussions that took place during the workshop. Over the two day workshop, several themes covered included: The microbiome is integral to human physiology, health, and disease. The microbiome is arguably the most intimate connection that humans have with their external environment, mostly through diet. Given the emerging nature of research on the microbiome, some important methodology issues might still have to be resolved with respect to undersampling and a lack of causal and mechanistic studies. Dietary interventions intended to have an impact on host biology via their impact on the microbiome are being developed, and the market for these products is seeing tremendous success. However, the current regulatory framework poses challenges to industry interest and investment.

Download or read book Diarrhea and Malnutrition written by Lincoln Chen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are several reasons why a consolidation of recent advances in our understanding of the interaction of diarrhea and malnutrition is indi cated and timely. It is now widely recognized that diarrhea is a major cause of morbidity and mortality among children of poor countries. Due to recent advances in laboratory and field diagnostic techniques, many of the previously unrecognized etiologic agents responsible for diarrhea have been identified, thereby providing new scientific knowledge for rational control strategies. Increasingly these advances suggest that the morbidity burden of diarrhea may be of equal, if not greater, public health consequence than mortality. Diarrhea only rarely causes disease severe enough to require institutionalized medical care. The vast major ity of diseases are of mild or moderate severity, and because of high prev alence, diarrhea imposes an enormous morbidity burden and exerts a sig nificant negative impact on child growth and development. Moreover, the effects of successive episodes of diarrhea are likely to be cumulative. In contrast to several other childhood infections, the treatment of the diarrheal diseases is feasible because it uses simple, effective, and low cost medical technologies. Within the context of these developments, there has been a major resurgence of international interest in, and commitment to, the control of the diarrheal diseases. The World Health Organization recently has launched a global program for the control of diarrhea, and simulta neously, an independent international research center on diarrhea has been established in Bangladesh.

Download or read book Eukaryome Impact on Human Intestine Homeostasis and Mucosal Immunology written by Nancy Guillen and published by Springer Nature. This book was released on 2020-06-01 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multiple demographic or economic parameters contribute to the origin of emerging infections, for example: poverty, urbanization, climate change, conflicts and population migrations. All these factors are a challenge to assess the impact (present and future) of parasitic diseases on public health. The intestine is a major target of these infections; it is a nutrient-rich environment harbouring a complex and dynamic population of 100 trillion microbes: the microbiome. Most researches on the microbiome focus on bacteria, which share the gut ecosystem with a population of uni- and multi cellular eukaryotic organisms that may prey on them. Our interest focuses on the families of eukaryotic microbes inhabiting the intestine, called “intestinal eukaryome”, that include fungi, protists and helminths. Knowledge on the reciprocal influence between the microbiome and the eukaryome, and on their combined impact on homeostasis and intestinal diseases is scanty and can be considered as an important emerging field. Furthermore, the factors that differentiate pathogenic eukaryotes from commensals are still unknown. This book presents an overview of the science presented and discussed in the First Eukaryome Congress held from October 16th to 18th, 2019 at the Pasteur Institute in Paris. This book covers the following topics: Phylogenetic, prevalence, and diversity of intestinal eukaryotic microbes; and their (still enigmatic) historical evolution and potential contributions to mucosal immune homeostasis. Integrative biology to study the molecular cell biology of parasite-host interactions and the multiple parameters underlining the infectious process. The exploitation of tissue engineering and microfluidics to establish three-dimensional (3D) systems that help to understand homeostasis and pathological processes in the human intestine.

Download or read book Prebiotics and Probiotics Science and Technology written by Dimitris Charalampopoulos and published by Springer Science & Business Media. This book was released on 2009-08-12 with total page 1273 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive overview on the advances in the field, this volume presents the science underpinning the probiotic and prebiotic effects, the latest in vivo studies, the technological issues in the development and manufacture of these types of products, and the regulatory issues involved. It will be a useful reference for both scientists and technologists working in academic and governmental institutes, and the industry.

Download or read book Dietary Fibre Functionality in Food and Nutraceuticals written by Farah Hosseinian and published by John Wiley & Sons. This book was released on 2017-03-06 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Increasing fiber consumption can address, and even reverse the progression of pre-diabetes and other associated non-communicable diseases. Understanding the link between plant dietary fiber and gut health is a small step in reducing the heavy economic burden of metabolic disease risks for public health. This book provides an overview of the occurence, significance and factors affecting dietary fiber in plant foods in order to critically evaluate them with particular emphasis on evidence for their beneficial health effects.

Download or read book Immunobiotics Interactions of Beneficial Microbes with the Immune System written by Julio Villena and published by Frontiers Media SA. This book was released on 2018-01-26 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The term “immunobiotics” has been proposed to define microbial strains able to beneficially regulate the mucosal immune system. Research in immunobiotics has significantly evolved as researchers employed cutting-edge technologies to investigate the complex interactions of these beneficial microorganisms with the immune system. During the last decade, our understanding of immunobiotics-host interaction was profoundly transformed by the discovery of microbial molecules and host receptors involved in the modulation of gut associated immune system, as well as the systemic and distant mucosal immune systems. In recent years, there has been a substantial increase in the number of reports describing the beneficial effects of immunobiotics in diseases such as intestinal and respiratory infections, allergy, inflammatory bowel disease, obesity, immunosuppression, and several other immune-mediated conditions. Evidence is also emerging of immunobiotics related molecules with immunomodulatory functions leading to the production of pharmabiotics, which may positively influence human or animal health. Therefore, research in immunobiotics continue to contribute not only to food but also medical and pharmaceutical fields. The compilation of research articles included in this ebook should help reader to have an overview of the recent advances in immunobiotics.

Download or read book Crohn s Disease and Ulcerative Colitis written by Daniel C. Baumgart and published by Springer. This book was released on 2017-03-01 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition is a unique combined resource for physicians and scientists addressing the needs of both groups. In addition to stimulating exchange and collaboration and shortening the path between discovery and application of new knowledge, the book helps clinicians understand new therapeutic concepts from their origins. The volume serves as a comprehensive guide to the current diagnostic modalities, including enhanced imaging techniques such as MRI and CT enterography, virtual colonoscopy, ultrasound, and endomicroscopy, as well as conventional and complex immunomodulatory principles. The latest edition also includes revised chapters from the previous edition, as well as new chapters reflecting current developments in the field. Written by experts in their field, Crohn’s Disease and Ulcerative Colitis: From Epidemiology and Immunobiology to a Rational Diagnostic and Therapeutic Approach, Second Edition is of great value to gastroenterologists, surgeons, internists, pediatricians and gynecologists trainees, as well as all those involved in Crohn’s disease, ulcerative colitis, and related autoimmune disorders.

Download or read book The Gut Microbiome in Health and Disease written by Dirk Haller and published by Springer. This book was released on 2018-07-27 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides an overview on how the gut microbiome contributes to human health. The readers will get profound knowledge on the connection between intestinal microbiota and immune defense systems. The tools of choice to study the ecology of these highly-specialized microorganism communities such as high-throughput sequencing and metagenomic mining will be presented. In addition the most common diseases associated to the composition of the gut flora are discussed in detail. The book will address researchers, clinicians and advanced students working in biomedicine, microbiology and immunology.

Download or read book Human Microbiome written by and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Oral Biofilms written by S. Eick and published by Karger Medical and Scientific Publishers. This book was released on 2020-12-21 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofilms are highly organized polymicrobial communities that are embedded in an extracellular matrix and formed on natural and artificial surfaces. In the oral cavity, biofilms are formed not only on natural teeth, but also on restorative materials, prosthetic constructions, and dental implants. Oral diseases like caries, gingivitis, periodontitis, and also pulp inflammation are associated with biofilms. This publication is an up-to-date overview on oral biofilms from different clinically relevant perspectives. Experts comprising basic researchers and clinicians report on recent research relating to biofilms - from general summaries to recommendations for daily clinical work. This book covers all aspects of oral biofilms, including models used in the laboratory, biofilms in dental water unit lines, periodontal and peri-implant biofilms, caries-related biofilms, halitosis, endodontic biofilms, and Candida infections, as well as biofilms on dental materials and on orthodontic appliances. Several chapters deal with anti-biofilm therapy, from the efficacy of mechanical methods and the use of antimicrobials, to alternative concepts. This publication is particularly recommended to dental medicine students, practitioners, other oral healthcare professionals, and scientists with an interest in translational research on biofilms.

Download or read book Management of the Child with a Serious Infection Or Severe Malnutrition written by World Health Organization. Department of Child and Adolescent Health and Development and published by World Health Organization. This book was released on 2000 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is for use by doctors, senior nurses and other senior health workers who are responsible for the care of young children at the first referral level in developing countries. It presents up-to-date expert clinical guidelines for both inpatient and outpatient care in small hospitals where basic laboratory facilities and essential drugs and inexpensive medicines are available. The manual focuses on the inpatient management of the major causes of childhood mortality, such as pneumonia, diarrhoea, severe malnutrition,, malaria, meningitis, measles, and related conditions. Descriptions of expensive treatment options are deliberately omitted from the manual.

Download or read book The Role of Biofilms in Device Related Infections written by Mark Shirtliff and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approximately 60% of all hospital-associated infections, over one million cases per year, are due to biofilms that have formed on indwelling medical devices. Device-related biofilm infections increase hospital stays and add over one billion dollars/year to U.S. hospitalization costs. Since the use and the types of indwelling medical devices commonly used in modern healthcare are continuously expanding, especially with an aging population, the incidence of biofilm infections will also continue to rise. The central problem with microbial biofilm infections of foreign bodies is their propensity to resist clearance by the host immune system and all antimicrobial agents tested to date. In fact, compared to their free floating, planktonic counterparts, microbes within a biofilm are 50 – 500 times more resistant to antimicrobial agents. Therefore, achieving therapeutic and non-lethal dosing regimens within the human host is impossible. The end result is a conversion from an acute infection to one that is persistent, chronic, and recurrent, most often requiring device removal in order to eliminate the infection. This text will describe the major types of device-related infections, and will explain the host, pathogen, and the unique properties of their interactions in order to gain a better understanding of these recalcitrant infections.

Download or read book Liver Immunology written by M. Eric Gershwin and published by Springer Science & Business Media. This book was released on 2013-11-19 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liver Immunology: Principles and Practice, Second Edition begins with important information about the epidemiology and mortality of liver disease worldwide. This information is followed by chapters related to basic immunology, application of liver immunology for diagnosis, and several excellent chapters that provide a solid foundation for understanding immune-mediated liver disease, including those associated with the biliary tree. A chapter on non-hepatic manifestations of immune mediated liver disease helps provide context for how these diseases affect the patient overall. In addition, chapters discuss various discrete immunologically-mediated infectious liver disorders including those related to bacteria, parasites, and all of the classic viruses. Chapters on the traditional autoimmune liver diseases -- primary biliary cirrhosis, autoimmune hepatitis, primary sclerosing cholangitis as well as overlap syndrome – are also included. The breadth of this comprehensive second edition is highlighted by chapters on alcoholic liver disease, non-alcoholic fatty liver disease, and drug-induced liver disease, among others. This invaluable new edition ends with a forward-looking view of future directions and how the field might meet the challenge of refractory patients. Developed by a renowned group of authors, Liver Immunology: Principles and Practice, Second Edition will again serve as a comprehensive textbook by providing an excellent overview for this rapidly evolving field. It greatly adds to the understanding of the pathogenesis of these diseases, while also providing novel insights that can be harnessed into helping improve the care of patients afflicted with various immune-mediated diseases. This volume will again be a must-read for clinicians at all levels, investigators and students.

Download or read book Microbial Products for Health Environment and Agriculture written by Pankaj Kumar Arora and published by Springer Nature. This book was released on 2021-09-21 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited volume discusses the role of various microbial products in healthcare, environment and agriculture. Several microbial products are directly involved in solving major health problems, agricultural and environmental issues. In healthcare sector, microbes are used as anti-tumor compounds, antibiotics, anti-parasitic agents, enzyme inhibitors and immunosuppressive agents. Microbial products are also used to degrade xenobiotic compounds and bio-surfactants, for biodegradation process. In agriculture, microbial products are used to enhance nutrient uptake, to promote plant growth, or to control plant diseases. The book presents several such applications of microbes in the ecosystems. The chapters are contributed from across the globe and contain up-to-date information. This book is of interest to teachers, researchers, microbiologists and ecologists. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

Download or read book Foodborne Microbial Pathogens written by Arun Bhunia and published by Springer Science & Business Media. This book was released on 2007-11-22 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: At last, here is a graduate-level textbook that focuses on the very latest information on the molecular and cellular mechanism of several major foodborne bacterial pathogens. For the first time in the field, this book makes the link between foodborne illness and immunology. It also covers virulence genes and their regulation in the host or the food environment, pathogenicity testing models, clinical symptoms and prevention and control strategies. Unlike other textbooks this one also covers the host/parasite interaction to a level where readers have a real appreciation of the disease mechanism. It is imperative that we acquire a better understanding of foodborne pathogens. And this is what this brilliant and timely contribution to the subject offers.

Download or read book Sex and Gender Factors Affecting Metabolic Homeostasis Diabetes and Obesity written by Franck Mauvais-Jarvis and published by Springer. This book was released on 2017-12-08 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides a reference for years to come, written by world-renowned expert investigators studying sex differences, the role of sex hormones, the systems biology of sex, and the genetic contribution of sex chromosomes to metabolic homeostasis and diseases. In this volume, leaders of the pharmaceutical industry present their views on sex-specific drug discovery. Many of the authors presented at the Keystone Symposium on “Sex and gender factors affecting metabolic homeostasis, diabetes and obesity” to be held in March 2017 in Lake Tahoe, CA. This book will generate new knowledge and ideas on the importance of gender biology and medicine from a molecular standpoint to the population level and to provide the methods to study them. It is intended to be a catalyst leading to gender-specific treatments of metabolic diseases. There are fundamental aspects of metabolic homeostasis that are regulated differently in males and females, and influence both the development of diabetes and obesity and the response to pharmacological intervention. Still, most preclinical researchers avoid studying female rodents due to the added complexity of research plans. The consequence is a generation of data that risks being relevant to only half of the population. This is a timely moment to publish a book on sex differences in diseases as NIH leadership has asked scientists to consider sex as a biological variable in preclinical research, to ensure that women get the same benefit of medical research as men.