Download or read book Bacterial Source Tracking in Impaired Watersheds written by Emily Catherine Martin and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial contamination due to excessive levels of bacteria is a confounding problem and remediation of impaired watersheds relies on the detection of fecal indicator bacteria and then assessing the source of said bacteria. Bacterial source tracking (BST) is an approach for assessing potential sources of this contamination. The purpose of this study was to utilize both cultivation-independent and -dependent methods to improve the ability to track sources of fecal contamination. First, E. coli community composition was assessed across three standard water quality assessments including USEPA Methods 1603 and 1604, and Colilert®, to determine their impact on BST library-based performance. Results indicate that the three assessed methods of enumeration and isolation may select for different populations of E. coli and standardized methods may be warranted if library-dependent BST is part of a research plan. Next, BST techniques were used to enumerate and characterize E. coli communities across various dairy manure management techniques used in the Leon River watershed in central Texas to determine effectiveness of BST efforts in tracking contamination from dairy manure. Results of this study indicated that manure and effluent management strategies which employed means to remove solids from the manure tended to decrease the levels of E. coli in the effluent. Some E. coli genotypes were found across the managerial treatments even though there were no clear seasonal trends or site groupings among the dataset. The vast majority of the isolates classified using the Texas E. coli BST library were correctly classified back to their major source class, thus increasing confidence in the methods currently being utilized to track dairy fecal contributions in this Central Texas watershed. Finally, deer bacterial fecal communities from south and central Texas were analyzed using 454-pyrosequencing to assess the potential for the development of a deer-specific BST marker. Microbial communities did not cluster by site or year suggesting that deer fecal communities in these Texas regions are stable over time and could be amenable to marker development. The electronic version of this dissertation is accessible from http://hdl.handle.net/1969.1/149494

Download or read book Microbial Source Tracking written by Jorge W. Santo Domingo and published by Emerging Issues in Food Safety. This book was released on 2007 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.

Download or read book Evaluation of Selected DNA based Technology in Impaired Watersheds Impacted by Fecal Contamination from Diverse Sources written by Marirosa Molina and published by . This book was released on 2007 with total page 47 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fecal pollution of surface waters is a top reason for impairment, as reported in the U.S. Environmental Protection Agency's report on the quality of the Nations waters. To be able to develop and implement TMDLs for impaired aquatic resources, it is imperative to determine the sources of the contamination. One tool used to determine the sources of bacterial fecal contamination is to apply a microbial source tracking approach to the system of interest. Microbial source tracking (MST) approaches are based on the assumption that specific strains of bacteria, genetic fingerprints, or DNA-based markers are associated with specific host species. Because accurate source identification of fecal contamination is essential in MST, more sensitive, selective and reliable molecular markers are required. The two types of genotypic methods that have been applied widely in a variety of environments can be classified as library-independent (LI) and library-dependent (LD). For both types, the temporal and spatial stability of selected genotypes are aspects that need to be evaluated, and these aspects are often times missing when applying MST to environmental samples. LD-MST methods require the development of large databases comprised of source-specific isolates. Once a source-specific fingerprint has been identified, the temporal and spatial variability of that particular genotype still needs to be validated. LI-MST is based on the application of culture-independent methods such as amplification of DNA from environmental samples using 16S rDNA markers in combination with polymerase chain reaction (PCR).

Download or read book Microbial Source Tracking written by Michèle Gourmelon and published by Frontiers Media SA. This book was released on 2022-01-18 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbial Source Tracking Methods Applications and Case Studies written by Charles Hagedorn and published by Springer Science & Business Media. This book was released on 2011-06-08 with total page 645 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the origin of fecal pollution is essential in assessing potential health risks as well as for determining the actions necessary to remediate the quality of waters contaminated by fecal matter. As a result, microbial source tracking (MST) has emerged as a field that has evolved and diversified rapidly since the first approaches were described only a decade ago. In response to the emergence of MST, there have been three large multi-laboratory method comparison studies (two in the US and one in Europe), plus numerous workshops, book chapters, and review articles dedicated to synthesizing information on the topic. Furthermore, a federal (USEPA) guide document describing the uses and limitations of MST methods was published in 2005, and a book dedicated to MST as an emerging issue in food safety was published in 2007. These documents provide a collective body of literature on MST that is both conflicting and complementary, often repetitious, and difficult to condense and interpret. In addition, it does not reflect the current diversity of MST approaches with different organisms, newer methodologies such as quantitative PCR, and anthropogenic chemicals, nor does it embrace the scope of MST research being conducted around the world. The three editors of the book, all with extensive MST expertise, have developed chapters and invited authors who reflect the rich diversity and truly international scope of MST. The unifying theme throughout the book is the design of more standardized approaches to MST that include performance criteria (regardless of method or organism), plus recommendations for field study design and MST implementation. The editors intend that this book will serve as a valuable reference for all those who are involved with

Download or read book An Investigation Into Bacterial Contamination in an Urban Nebraska Stream Using Microbial Source Tracking written by Allison R. Speicher and published by . This book was released on 2015 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: High levels of fecal bacteria in surface waters are a public health concern due to the enteric illnesses caused by primary contact with these waters. Shortcomings in the current fecal indicator bacteria (FIB) monitoring approach and the ubiquity of pathogen-impaired water bodies has led to the development of microbial source tracking (MST), a molecular tool that can identify potential fecal contamination sources. In this study we use a bacterial community based approach examining the V4 region of the 16S rRNA gene to elucidate sources of fecal bacteria in Antelope Creek, an urban creek in Lincoln Nebraska. We hypothesized that sediment from the bed of Antelope Creek and animal wastes near the stream were major sources of the fecal bacteria in dry and wet weather water samples. To test the hypothesis, water from three dry weather events and three storm events was collected in addition to streambed sediment, sanitary sewage, and fecal waste from birds, dogs, small mammals, and horses. A UniFrac multi-variative principal coordinate analysis (PCoA) revealed differences in bacterial communities from sediment and water samples, indicating that sediment is not likely a major contributor of bacteria to dry and wet weather flows. PCoA analysis on the Bacteroidetes phylum revealed strong differences in community composition between water samples and fecal waste from horses, sanitary sewage, geese and dogs. The closest fecal community compositions were found for wastes from small mammals, pigeons, and ducks. Statistical differences were seen in the overall bacterial community and the Bacteroidetes population by sampling date for both dry and wet weather water, while no statistical differences were seen by sampling location. This distinction suggests that environmental or source changes throughout the recreational season (e.g. temperature, sunlight, seasonal animal migration) were the major drivers of the shifts in the bacterial community in Antelope Creek. The absence of a location with consistently high E. coli counts suggests no major point source contribution.

Download or read book Use of the Hydrological Simulation Program FORTRAN and Bacterial Source Tracking for Development of the Fecal Coliform Total Maximum Daily Load TMDL for Blacks Run Rockingham County Virginia written by Douglas L. Moyer and published by . This book was released on 2003 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regional Cooperation for Water Quality Improvement in Southwestern Pennsylvania written by National Research Council and published by National Academies Press. This book was released on 2005-04-04 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: The city of Pittsburgh and surrounding area of southwestern Pennsylvania face complex water quality problems, due in large part to aging wastewater infrastructures that cannot handle sewer overflows and stormwater runoff, especially during wet weather. Other problems such as acid mine drainage are a legacy of the region's past coal mining, heavy industry, and manufacturing economy. Currently, water planning and management in southwestern Pennsylvania is highly fragmented; federal and state governments, 11 counties, hundreds of municipalities, and other entities all play roles, but with little coordination or cooperation. The report finds that a comprehensive, watershed-based approach is needed to effectively meet water quality standards throughout the region in the most cost-effective manner. The report outlines both technical and institutional alternatives to consider in the development and implementation of such an approach.

Download or read book Use of the Hydrological Simulation Program FORTRAN and Bacterial Source Tracking for Development of the Fecal Coliform Total Maximum Daily Load TMDL for Accotink Creek Fairfax County Virginia written by Douglas L. Moyer and published by . This book was released on 2003 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Use of the Hydrological Simulation Program FORTRAN and Bacterial Source Tracking for Development of the Fecal Coliform Total Maximum Daily Load TMDL for Christians Creek Augusta County Virginia written by Douglas L. Moyer and published by . This book was released on 2003 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbial Source Tracking in the South Dry Sac Watershed written by Emily L. Sturnfield and published by . This book was released on 2011 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: Karst watersheds are an important resource that can be easily contaminated. The South Dry Sac Watershed near Springfield, MO presents a unique opportunity to study the combined effects of urban and rural runoff on a karst river system, as the system changes from rural to urban within the span of a few kilometers. Previous studies have indicated that the watershed is impaired by fecal bacteria, but sources of the bacteria were not investigated. This study traced sources of fecal coliform bacteria (bovine, human) in the watershed and related it to land use. Bovine source Bacteroides was prevalent throughout the field area, while human source Bacteroides was present in lower levels. Results indicate higher human fecal loading at sites located near areas using septic waste treatment. Additional water quality parameters helped distinguish water signatures of the urban from the rural areas within the watershed. The two most contaminated sites were tracked to both bovine and human sources, were downstream from known larger-scale cattle operations, and received drainage from unsewered portions of the study area. The range of values obtained and their variation with time provides a good framework for watershed management.

Download or read book Evaluation and Performance of Rapid Methods for Identifying and Tracking Sources of Fecal Pollution in Coastal Watersheds written by Vanessa Thulsiraj and published by . This book was released on 2014 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fecal contamination of coastal waters is known to degrade the environment and poses a health risk to recreational beach users. Fecal indicator bacteria (FIB) are used around the world to assess water quality and characterize fecal contamination. Elevated levels of FIB have been linked to health risks in epidemiological studies. However, some limitations exist with this indicator. FIB cannot be used to identify the specific sources as they originate from both human and animal sources. FIB may also persist and regrow in the environment. In order to effectively remediate the cause of pollution and characterize the hazards at chronically impaired beaches it is necessary to measure indicators that can provide information about the sources of the general fecal pollution. Tracking pollution sources at impaired beaches is critical to ensuring the health of coastal watersheds and reducing the incidence of swimming related illness. Molecular methods have gained popularity to identify and detect sources of fecal contamination using host-associated markers. The work presented here addresses areas warranting further research in the state of the science of water quality monitoring. In Chapter 2, we demonstrate that host-associated markers exhibit similar limits of detection in different water types and are robust in environmental field applications. Additionally, we provide a cost-benefit analysis and provide water quality managers with information supporting the inclusion of molecular methods in current monitoring practices. This body of work also presents novel methods for rapid and viability-based detection of recent fecal contamination with propidium monoazide (PMA-qPCR) and a field portable method covalently-linked IMS/ATP technique (Cov-IMS/ATP). In Chapters 3 and 4, we present results on optimization and specificity of the Cov-IMS/ATP. We evaluated the performance of Cov-IMS/ATP at three different watersheds for rapid quantification of enterococci, and show this method to be a robust tool in assessing water quality at complex sites. This work also addresses drawbacks of traditional qPCR to quantify viable fecal contamination. We validate the PMA-qPCR method and demonstrate its performance in detecting recent fecal contamination in environmental waters. Use of these methods demonstrates a new framework that can enhance current microbial source tracking studies and water quality monitoring.

Download or read book Water resources Investigations Report written by Douglas L. Moyer and published by . This book was released on 2003 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Water resources Investigations Report written by and published by . This book was released on 2003 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Waterborne Pathogens written by Majeti Narasimha Vara Prasad and published by Butterworth-Heinemann. This book was released on 2020-02-07 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Waterborne Pathogens: Detection and Treatment delivers the tools and techniques on how to identify these contaminates and apply the most effective technology for their removal and treatment. Written for researchers and practicing professionals, the book starts with a brief, but readable, review of ubiquitous waterborne pathogens (primarily viruses, bacterial and parasitic protozoa). This coverage is followed by an in-depth discussion of the latest detection and treatment technologies, ranging from Biosensors, to Nanoconjugates, Membrane Based Technologies and Nanotechnology Treatment. Engineers and scientist will find this to be a valuable reference on cutting-edge techniques for suppling safe drinking water across the globe. - Explains the latest research on detection, treatment processes and remediation technologies - Includes sampling, analytical and characterization methods and approaches - Covers cutting-edge research, including Membrane Based Technologies, Nanotechnology Treatment Technologies and Bioremediation Treatment Technologies - Provides background information regarding contamination sources

Download or read book Discrimination of Human and Non human Fecal Sources with Rapid Methods in Coastal Waters and Sediments written by Amity Gayle Zimmer-Faust and published by . This book was released on 2015 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial contamination in coastal waters is an important public health and economic problem worldwide. It is common for the sources as well as the environmental fate and transport of fecal contamination in recreational waters to be unknown making it difficult to mitigate the input and to understand the relative health risk associated with a given water body. A comprehensive understanding of sources and bacterial dynamics is needed for effective mitigation and management of microbial contaminants. The main objectives of this study were to identify sources of fecal contamination to a chronically impaired Southern California watershed, to demonstrate applicability of microbial source tracking (MST) tools, including source-specific markers for discrimination of human and non-human sources, to evaluate the fate of fecal contaminates in coastal sediments, and to develop and optimize immunomagnetic separation/adenosine triphosphate (IMS/ATP) assays for rapid enumeration of viable fecal contamination. A three-year MST study was conducted to help explain elevated levels of surfzone fecal indicator bacteria (FIB) at Topanga State Beach, a critically impaired Southern California Beach. This study investigated sources of FIB to the Topanga watershed and the applicability of using MST technology longer time scales. MST markers effectively elucidated temporal and seasonal trends in fecal bacterial levels, and dog and gull marker appeared to be a significant sources to Topanga lagoon and Topanga State Beach. However, a lack of correlation between FIB and marker measurements was noted, and dog marker and FIB levels did not covary when compared at different Southern California beaches. Sediments were found to play an important and variable role in environmental fate of MST markers and FIB. Variable decay was observed for different indicators and in different sediments, with differences noted even within one watershed. The human HF183 marker was useful for providing evidence of recent inputs of human fecal contamination and behaved similarly to the molecular marker for Campylobacter (qCAMP) and FIB in brackish sediments. The general Bacteroides (GB3) and enterococci (ENT1A) markers were more conservative and under certain circumstances had comparable decay to culturable FIB. Application of a suite of markers may be necessary for effective evaluation of sediment fecal bacterial levels. Moreover, differences were observed between relative decay amongst the different sediments tested, illustrating the need for more routine sediment monitoring. IMS/ATP assays provided useful information regarding fecal contamination levels and measurements made had a consistent relationship with measurements made by standard methods. IMS/ATP utilizes paramagnetic beds and target-specific antibodies to isolate target organisms. Following isolation, adenosine tri-phosphate (ATP) is extracted from the target population and quantified. The Cov-IMS/ATP method rapidly measured viable enterococci in complex surface waters, providing a useful eld tool for assessment of coastal water quality and for identi cation of hot spots of fecal contamination. An inversely-coupled (Inv-IMS/ATP) assay for detection of Bacteroides thetaiotaomicron was developed and applied for rapid detection of human-associated fecal contamination. The Inv-IMS/ATP assay yielded measurements of viable B. thetaiotaomicron that were comparable to the HF183 human marker in complex source waters impacted with both wastewater and runoff, and the Inv-IMS/ATP assay was able to effectively differentiate between surface waters impacted with adequately and inadequately treated wastewater. IMS/ATP assays show promise for rapid evaluation of recreational water quality in areas where access to more expensive methods is limited and in areas where water quality is unpredictable. This research highlights the difficulties and complexities associated with effective tracking and management of microbial contaminates in the coastal environment. Additional research evaluating relative aging of molecular markers and relative contributions from different sources is needed to fully interpret field-based source marker data. Sediments were shown to have an important and variable role in fate of fecal contaminants in the environment. Additional studies are needed evaluating how watershed models can most effectively be adapted to include a sediment compartment and how different sources of fecal contamination and source markers decay in sediments with variable characteristics. IMS/ATP assays showed promise and can be successfully applied in complex waters for rapid enumeration of viable fecal contamination; additional verification of assay performance is needed at complex sites impacted with multiple sources.

Download or read book Patterns and Sources of Fecal Coliform Bacteria in Three Streams in Virginia 1999 2000 written by Kenneth Edward Hyer and published by . This book was released on 2003 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: