Download or read book Avidin Biotin Interactions written by Robert J. McMahon and published by Springer Science & Business Media. This book was released on 2008-02-26 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.
Download or read book Immunoenzymatic Techniques written by S. Avrameas and published by Elsevier Science & Technology. This book was released on 1983 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aandacht voor de enzymatische-immunocytochemie met de nadruk op intracellulaire penetratie van gelabelde antilichamen en de toepassing van "double staining"-methodes of monoclonale antilichamen in normale en in diverse experimentele of pathologische omstandigheden; kwantitatieve aspecten van immuno-enzymatische technieken, zoals nieuwe technische vindingen en toepassingen op verschillende gebieden, bijvoorbeeld biologie, humane en veterinaire geneeskunde, fytopathologie en experimentele ziekten
Download or read book Avidin biotin Technology written by Meir Wilchek and published by . This book was released on 1990 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Download or read book Methods for Fragments Screening Using Surface Plasmon Resonance written by Sameer Mahmood Zaheer and published by Springer. This book was released on 2022-09-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
Download or read book Immobilised Macromolecules Application Potentials written by U.B. Sleytr and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized functional biomolecules, particularly enzymes, are important tools in biotechnology, biochemistry, biochemical engineering, biomedicine and biosensor research. This book provides an introduction and overview of selected major areas of the science and technology of immobilized systems. The chapters are intended as an introduction and overview to these interdisciplinary areas, as well as a source of practical details and of new research trends. This book will be useful for scientists, technologists, academics and students in direct and related fields.
Download or read book Immunocytochemical Methods and Protocols written by Lorette C. Javois and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
Download or read book Proteomic and Metabolomic Approaches to Biomarker Discovery written by Haleem J. Issaq and published by Academic Press. This book was released on 2013-05-20 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. Describes the use of biomarkers to reduce clinical errors in research Includes techniques from a range of biomarker discoveries Covers all steps involved in biomarker discovery, from study design to study execution
Download or read book Graphene Field Effect Transistor Biosensors written by Shiyu Wang and published by Springer Nature. This book was released on 2021-03-31 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this monograph, the graphene-based field-effect transistor (FET) biosensors are shown to be an emerging sensing platform. Divided into two parts the first set of chapters are devoted to basic knowledge of graphene, graphene FET and its biosensing. In the second part of this book the applications of graphene FET biosensors combined with various biotechnologies are presented. As well as discussing the existing technologies the authors also introduce their own ideas and concepts. Finally the remaining problems in graphene FET biosensors are discussed, along with proposed solutions and prospects for future applications. This monograph allows readers to grasp the basic knowledge and future direction of graphene-based FET biosensors.
Download or read book Nanobiosensors written by Alexandru Grumezescu and published by Academic Press. This book was released on 2016-12-20 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanobiosensors: Nanotechnology in the Agri-Food Industry, Volume 8, provides the latest information on the increasing demand for robust, rapid, inexpensive, and safe alternative technologies that monitor, test, and detect harmful or potentially dangerous foods. Due to their high sensitivity and selectivity, nanobiosensors have attracted attention for their use in monitoring not only biological contaminants in food, but also potential chemical and physical hazards. This book offers a broad overview regarding the current progress made in the field of nanosensors, including cutting-edge technological progress and the impact of these devices on the food industry. Special attention is given to the detection of microbial contaminants and harmful metabolotes, such as toxins and hormones, which have a great impact on both humans and animal health and feed. Includes the most up-to-date information on nanoparticles based biosensors and quantum dots for biological detection Provides application methods and techniques for research analysis for bacteriological detection and food testing Presents studies using analytical tools to improve food safety and quality analysis
Download or read book Nutrition and Lifestyle for Pregnancy and Breastfeeding written by Peter Gluckman and published by Oxford University Press, USA. This book was released on 2015 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explaining the practical implications of new discoveries in life-course biology, this is an informed resource on factors that affect offspring development.
Download or read book Cell Surface Engineering written by Rawil Fakhrullin and published by Royal Society of Chemistry. This book was released on 2014-07-24 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: A summary of the recent achievements in surface-functionalised cells including fabrication, characterisation, applications and nanotoxicity.
Download or read book Avidin biotin Chemistry written by M. Dean Savage and published by . This book was released on 1992-01-01 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Supramolecular Catalysis written by Piet W.N.M. van Leeuwen and published by John Wiley & Sons. This book was released on 2021-12-31 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: Supramolecular Catalysis Provides a timely and detailed overview of the expanding field of supramolecular catalysis The subdiscpline of supramolecular catalysis has expanded in recent years, benefiting from the development of homogeneous catalysis and supramolecular chemistry. Supramolecular catalysis allows chemists to design custom-tailored metal and organic catalysts by devising non-covalent interactions between the various components of the reaction. Edited by two world-renowned researchers, Supramolecular Catalysis: New Directions and Developments summarizes the most significant developments in the dynamic, interdisciplinary field. Contributions from an international panel of more than forty experts address a broad range of topics covering both organic and metal catalysts, including emergent catalysis by self-replicating molecules, switchable catalysis using allosteric effects, supramolecular helical catalysts, and transition metal catalysis in confined spaces. This authoritative and up-to-date volume: Covers ligand-ligand interactions, assembled multi-component catalysts, ligand-substrate interactions, and supramolecular organocatalysis and non-classical interactions Presents recent work on supramolecular catalysis in water, supramolecular allosteric catalysis, and catalysis promoted by discrete cages, capsules, and other confined environments Highlights current research trends and discusses the future of supramolecular catalysis Includes full references and numerous figures, tables, and color illustrations Supramolecular Catalysis: New Directions and Developments is essential reading for catalytic chemists, complex chemists, biochemists, polymer chemists, spectroscopists, and chemists working with organometallics.
Download or read book Emerging Areas in Bioengineering written by Ho Nam Chang and published by John Wiley & Sons. This book was released on 2018-03-12 with total page 898 pages. Available in PDF, EPUB and Kindle. Book excerpt: With more than 40 contributions from expert authors, this is an extensive overview of all important research topics in the field of bioengineering, including metabolic engineering, biotransformations and biomedical applications. Alongside several chapters dealing with biotransformations and biocatalysis, a whole section is devoted to biofuels and the utilization of biomass. Current perspectives on synthetic biology and metabolic engineering approaches are presented, involving such example organisms as Escherichia coli and Corynebacterium glutamicum, while a further section covers topics in biomedical engineering including drug delivery systems and biopharmaceuticals. The book concludes with chapters on computer-aided bioprocess engineering and systems biology. This is a part of the Advanced Biotechnology book series, covering all pertinent aspects of the field with each volume prepared by eminent scientists who are experts on the topic in question. Invaluable reading for biotechnologists and bioengineers, as well as those working in the chemical and pharmaceutical industries. Advanced Biotechnology Biotechnology is a broad, interdisciplinary field of science, combining biological sciences and relevant engineering disciplines, that is becoming increasingly important as it benefits the environment and society as a whole. Recent years have seen substantial advances in all areas of biotechnology, resulting in the emergence of brand new fields. To reflect this progress, Sang-Yup Lee (KAIST, South Korea), Jens Nielsen (Chalmers University, Sweden), and Gregory Stephanopoulos (MIT, USA) have joined forces as the editors of a new Wiley-VCH book series. Advanced Biotechnology will cover all pertinent aspects of the field and each volume will be prepared by eminent scientists who are experts on the topic in question.
Download or read book Diagnostic Immunohistochemistry written by David J. Dabbs and published by Churchill Livingstone. This book was released on 2006 with total page 858 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers current information and guidance on immunohistochemical diagnoses in surgical pathology and cytopathology. The book is organized in a consistent format of organ systems, specifically designed to target diagnostic dilemmas in surgical pathology. Each chapter features an introduction and a systematic approach to the diagnostic entities of that organ system. Provides a reference for pathologists practicing diagnostic surgical pathology and cytopathology. Structured in the organ-system approach to tumor pathology, with each chapter capable of standing alone. Extensive references and full-color illustrations are included.
Download or read book Biodegradable Hydrogels for Drug Delivery written by Haesun Park and published by CRC Press. This book was released on 2011-07-08 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the Authors' Preface The advances made in the area of controlled drug delivery during the last two decades are remarkable ....Of the many polymeric materials, biodegradable hydrogels present unique advantages and opportunities in the development of ...delivery devices....We have undertaken the challenge of putting together information relevant to biodegradable hydrogels in one place. This book covers the mechanisms of biodegradation, types of biodegradable hydrogels,chemical and physical gels, chemical and enzymatic degradation, and examples of biodegradable drug delivery systems.
