Download or read book Automated DNA Sequencing and Analysis written by Mark D. Adams and published by Elsevier. This book was released on 2012-12-02 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects. - The field of automation in DAN sequencing and analysis is rapidly moving, this book fulfils those needs, reviews the history of the art and provides pointers to future development.
Download or read book Automated DNA Sequencing and Analysis written by Mark D. Adams and published by Elsevier. This book was released on 1994-06-29 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects. The field of automation in DAN sequencing and analysis is rapidly moving, this book fulfils those needs, reviews the history of the art and provides pointers to future development.
Download or read book DNA Sequencing Strategies written by Wilhelm Ansorge and published by Wiley-Liss. This book was released on 1997 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This outstanding lab bench reference to the technology of DNA sequencing offers a collection of concise sequencing strategies and cloning protocols. Concentrates on the most up-to-the-minute automated methods and advanced approaches. Preparing DNA for sequencing, sequencing single- doubled-stranded DNA and their variations, how to optimise the primers used, preparation of DNA sequencing gels and the actual collection of results, labelling of DNA fragments for sequencing and data analysis are among the topics covered.
Download or read book DNA Sequencing Protocols written by Annette M. Griffin and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of DNA Sequencing Protocols is to provide detailed practical procedures for the widest range of DNA sequencing meth ods, and we believe that all the vanguard techniques now being applied in this fast-evolving field are comprehensively covered. Sequencing technology has advanced at a phenomenal rate since the original methods were first described in the late 1970s and there is now a huge variety of strategies and methods that can be employed to determine the sequence of any DNA of interest. More recently, a large number of new and innovative sequencing techniques have been developed, including the use of such novel polymerases as Tag poly merase and Sequenase, the harnessing of PCR technology for linear amplification (cycle) sequencing, and the advent of automated DNA sequencers. DNA sequencing is surely one of the most important techniques in the molecular biology laboratory. Sequence analysis is providing an increasingly useful approach to the characterization of biological systems, and major multinational projects are already underway to map and sequence the entire genome of organisms, such as Escherichia coli, Saccharomyces cerevisiae, Caenorhabditis elegans, and Homo sapiens. Most scientists recognize the importance of DNA sequence data and perceive DNA sequencing as a valuable and indispensable aspect of their work. Recent technological advances, especially in the area of automated sequencing, have removed much of the drudg ery that was formerly associated with the technique, whereas innova tive computer software has greatly simplified the analysis and manipulation of sequence data.
Download or read book A Local Processor for Improving the Accuracy of Automated DNA Sequencing written by Murugathas Yuwaraj and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis-based automated identification of the Deoxyribo Nucleic Acid (DNA) sequence provides a means of detecting variations in the DNA of an organism at the nucleotide level. Accurate detection of these variations and the DNA sequence, in general, are limited by statistical variations in the DNA time-series and overlap of adjacent peaks due to limited resolution. In this thesis, a local maximum likelihood processor (L-DNA), which is based on a statistical model of the DNA time-series is developed. The L-DNA poor generates a finite set of hypothesized sequences. For each hypothesized sequence, model parameters estimated from the observed waveform are used to evaluate the likelihood of the hypothesized sequence being consistent with the observed waveform. The hypothesis with the highest likelihood is selected as the local sequence in the observed region. The performance of the L-DNA processor is evaluated using both simulated and real DNA data. Simulations show that in low resolution regions of the DNA data, which are observed towards the end of a sequencing run, the probability of error for the proposed processor is two orders of magnitude smaller than the commonly used Jansson-van Cittert iterative deconvolution method. The robustness of the processor is illustrated by sequentially extending the read length of a fragment of DNA sequence. The ability of the processor to detect heterozygous sites and partial mutation sites in low resolution regions is illustrated using 30 segments of DNA data where the proportion of the wild and mutant types is artificially controlled. The L-DNA processor correctly identifies 80% of the data, while a commercial base-calling program correctly detects only 33% of the data. It should be noted that the commercial base-calling program is specifically designed for the sequencer that was used to acquire the above mentioned data. The L-DNA processor provides a measure of confidence for each hypothesized sequence. This measure can be used to significantly reduce the amount of human intervention needed to edit results of automated DNA sequencing system in clinical applications.
Download or read book DNA Sequencing Protocols written by Colin A Graham and published by Humana Press. This book was released on 2001-01-10 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.
Download or read book Gegen Ablehnung der beyden gedruckten zu Regenspurg in der Bohtkampischen Sache von dem Herrn Grafen von Brockdorff bergebenen Memorialien abseiten der zur k nigl d n Hollstein Gl ckst dt Regierungs Canzley verordneten R the written by and published by . This book was released on 1729 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mapping and Sequencing the Human Genome written by National Research Council and published by National Academies Press. This book was released on 1988-01-01 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.
Download or read book Computational Methods in Automated DNA Sequencing written by Michael C. Giddings and published by . This book was released on 1997 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Automated DNA Sequencing written by J. A. Brumbaugh and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Automation in Large Scale DNA Sequencing written by Eric C. Buxton and published by . This book was released on 1996 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Automated DNA Sequencing written by John Albert Brumbaugh and published by . This book was released on 1992 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Local Processor for Improving the Accuracy of Automated DNA Sequencing written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Preprocessing of Gel Images for Automated DNA Sequencing written by Weian Huang and published by . This book was released on 1995 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Object oriented System for the Analysis of Automated DNA Sequencing Data written by Reece Kimball Hart and published by . This book was released on 1994 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Local Processor for Improving the Accuracy of Automated DNA Sequencing microform written by Murugathas Yuwaraj and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 2001 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
