Download or read book Assembly and Characterization of Complex Biomimetic Membranes written by Kanika Vats and published by . This book was released on 2009 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomimetic Lipid Membranes Fundamentals Applications and Commercialization written by Fatma N. Kök and published by Springer. This book was released on 2019-04-16 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles the fundamentals, applications and viable product strategies of biomimetic lipid membranes into a single, comprehensive source. It broadens its perspective to interdisciplinary realms incorporating medicine, biology, physics, chemistry, materials science, as well as engineering and pharmacy at large. The book guides readers from membrane structure and models to biophysical chemistry and functionalization of membrane surfaces. It then takes the reader through a myriad of surface-sensitive techniques before delving into cutting-edge applications that could help inspire new research directions. With more than half the world's drugs and various toxins targeting these crucial structures, the book addresses a topic of major importance in the field of medicine, particularly biosensor design, diagnostic tool development, vaccine formulation, micro/nano-array systems, and drug screening/development. Provides fundamental knowledge on biomimetic lipid membranes; Addresses some of biomimetic membrane types, preparation methods, properties and characterization techniques; Explains state-of-art technological developments that incorporate microfluidic systems, array technologies, lab-on-a-chip-tools, biosensing, and bioprinting techniques; Describes the integration of biomimetic membranes with current top-notch tools and platforms; Examines applications in medicine, pharmaceutical industry, and environmental monitoring.
Download or read book Advances in Biomembranes and Lipid Self Assembly written by and published by Academic Press. This book was released on 2020-04-21 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biomembranes and Lipid Self-assembly, Volume 31, formerly titled Advances in Planar Lipid Bilayers and Liposomes, provides a global platform for the study of cell membranes, lipid model membranes and lipid self-assemblies, from the micro- to the nanoscale. As planar lipid bilayers are widely studied due to their ubiquity in nature, this book presents research on their application in the formulation of biomimetic model membranes, and in the design of artificial dispersion of liposomes. Moreover, the book discusses how lipids self-assemble into a wide range of other structures, including micelles and the liquid crystalline hexagonal and cubic phases. Chapters in this volume present both original research and comprehensive reviews written by world leading experts and young researchers. Surveys recent theoretical and experimental results on lipid micro- and nanostructures Presents potential use applications, such as clinically relevant diagnostic and therapeutic procedures, biotechnology, pharmaceutical engineering and food products Includes both original research and comprehensive reviews written by world-leading experts and young researchers Provides a global platform for a broad community of experimental and theoretical researchers studying cell membranes, lipid model membranes, and lipid self-assemblies, from the micro- to the nanoscale
Download or read book Characterization of Biological Membranes written by Mu-Ping Nieh and published by Walter de Gruyter GmbH & Co KG. This book was released on 2019-07-22 with total page 647 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of membranes has become of high importance in the fields of biology, pharmaceutical chemistry and medicine, since much of what happens in a cell or in a virus involves biological membranes. The current book is an excellent introduction to the area, which explains how modern analytical methods can be applied to study biological membranes and membrane proteins and the bioprocesses they are involved to.
Download or read book Biofunctional Membranes written by D.A. Butterfield and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: ''Interesting with many useful ideas and references. It covers a broad range and it is a good introduction to this field.'' ---Analyst
Download or read book Analysis of Biomimetic Block Copolymer Membranes Used for Protein Incorporation written by Allen Schantz and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Integral membrane proteins carry out a vast range of transport, catalytic, signaling, and other functions with high reactivity and specificity. These proteins and synthetic mimics thereof are now widely studied for applications relevant to chemical engineers, such as membrane separations, catalysis, and sensors. These proteins structural stability depends on a network of hydrogen bonds maintained in the amphiphilic environment provided by the cell membrane but disrupted in solution. Thus, to apply these proteins in medicine and industry, we must design and optimize biomimetic membranes self assembled amphiphilic structures that serve as matrices to mimic the cell membrane and stabilize integral membrane proteins. Amphiphilic block copolymers recapitulate the self-assembled microstructures formed by lipids and surfactants used to stabilize membrane proteins, but have greater mechanical and chemical stability than these small amphiphiles. Further, membrane properties relevant to protein incorporation, such as thickness and hydrophobicity, can be adjusted by changing the degree of polymerization and monomer identities, making block copolymers an excellent material for biomimetic membrane design.The goal of my dissertation research was to better understand biomimetic membranes structure, synthesis, and interactions with proteins. The introduction provides a brief overview of biomimetic membranes, including the block copolymer membrane properties, membrane synthesis, and the interactions between the protein and matrix that can be tailored to optimize protein incorporation and stability. The first chapter describes time-resolved small-angle neutron scattering experiments used to examine the mechanism of membrane self-assembly via detergent dialysis. We show that mixed detergent/polymer micelle fragmentation and fusion control the rate of polymer exchange, and thus the formation of mixed polymer/protein/detergent aggregates that form membranes as detergent is removed. In the second chapter, we use molecular dynamics simulations to examine the nanoscale structure of biomimetic membranes formed from poly(1,2-butadiene)-poly(ethylene oxide) and poly(ethyl ethylene)-poly(ethylene oxide). These simulations allow us to examine membrane thickness and hydration, two properties relevant to protein incorporation. The third chapter examines whether we could synthesize biomimetic membranes from mass-produced Pluronic block copolymers. We show that a mixture of two such polymers, L121 and F127, can assemble into porous vesicles, so that they can be used for separations and as catalytic microreactors. The fourth chapter examines interactions that lead to bacterial membrane fusion by the cationic antimicrobial peptide from Moringa oleifera. This work provides a control for future work to examine the interactions between biomimetic membranes and incorporated proteins using coarse-grained molecular dynamics. Finally, the appendices provide supporting information for each chapter, as well as a report on the design requirements for high-pressure reverse osmosis, a potential application for biomimetic membranes.
Download or read book Development and Characterization of Biomimetic Membranes for Use in Environmental and Biomedical Applications written by Cory Jones and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomimetic membranes have a variety of applications in the environmental and biomedical realms. The focus of this work is on understanding transport of two types of membranes: 1) Lipid vesicle membranes with incorporated microbial opsins, proteins which pump ions in response to light. 2) Porous Graphene membranes modified with a lipid bilayer. Protein incorporated membranes are important for use in optogenetics. Stopped-flow and patch clamp are two measurement techniques used to characterize ion transport. Stopped-flow was limited in measuring ion activity due to limitations in commercial sodium fluorophores. Patch Clamp was found to be a viable alternative in measuring channel and pump activity of proteins embedded in Giant Unilamellar Vesicles (GUVs) grown through electroformation. Lipid modified graphene membranes were created for use in water desalination. Measurement techniques were developed to accompany a purpose-built diffusion cell which can accurately measure water permeability (A value) and salt permeability (B value). The A and B values were calculated for Forward Osmosis (FO) membranes, and are comparable to industry standards, confirming the ability of this technique to analyze other membranes. An estimate of past unmodified and modified lipid for the A and B values were made by normalizing the number and size of pores. Lipid modified membranes are estimated to provide an A value of 15.7 LMH/bar ((L/(m2 hr))/ bar) (40x increase), B value of 0.0047 LMH (64x decrease), and Water Flux of 26.3 LMH (1.5x increase) in comparison to FO membranes.
Download or read book Stabilization and Characterization of 2D and 3D Biomimetic Membranes written by Christian Rein and published by . This book was released on 2011 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The BAM Complex written by Susan Buchanan and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Download or read book Development and Characterization of Biomimetic Membrane Architecture for Single Molecule Sensors and Electrophysiological Studies written by Tae-Joon Jeon and published by . This book was released on 2008 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomimetic and Bioinspired Membranes for New Frontiers in Sustainable Water Treatment Technology written by Amira Abdelrasoul and published by BoD – Books on Demand. This book was released on 2017-12-06 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomimetic and bioinspired membranes are the most promising type of membrane for multiple usage scenarios, including commercial separation applications as well as water and wastewater treatment technologies. In recent years, aquaporin biomimetic membranes (ABMs) for water purification have raised considerable interest. These membranes display uniquely favorable properties and outstanding performances, such as diverse interactions, varied selective transport mechanisms, superior stability, high resistance to membrane fouling, and distinct adaptability. Biomimetic membranes would make a significant contribution to alleviate water stress, environmental threats, and energy consumption.
Download or read book Rational Design and Characterization of Innovative Multifunctional Biomimetic Materials written by Lia Rimondini and published by Frontiers Media SA. This book was released on 2021-04-13 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Journal of Research of the National Institute of Standards and Technology written by and published by . This book was released on 2001 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Interfacial Supramolecular Assemblies written by Johannes G. Vos and published by John Wiley & Sons. This book was released on 2003-07-11 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the supramolecular properties of molecular assemblies that contain a solid phase, offering an integrated approach to measurement and addressibility. * Offers an integrated approach to measurement and addressibility. * Features case studies describing the major devices developed using this technology. * The prospects for the future of interfacial supramolecular assemblies are considered.
Download or read book Advanced Technologies for Protein Complex Production and Characterization written by M. Cristina Vega and published by Springer Nature. This book was released on 2024 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zusammenfassung: This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization.
Download or read book Materials Nanoarchitectonics written by Katsuhiko Ariga and published by Elsevier. This book was released on 2023-12-15 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: Materials Nanoarchitectonics: From Integrated Molecular Systems to Advanced Devices provides the latest information on the design and molecular manipulation of self-organized hierarchically structured systems using tailor-made nanoscale materials as structural and functional units. The book is organized into three main sections that focus on molecular design of building blocks and hybrid materials, formation of nanostructures, and applications and devices. Bringing together emerging materials, synthetic aspects, nanostructure strategies, and applications, the book aims to support further progress, by offering different perspectives and a strong interdisciplinary approach to this rapidly growing area of innovation. This is an extremely valuable resource for researchers, advanced students, and scientists in industry, with an interest in nanoarchitectonics, nanostructures, and nanomaterials, or across the areas of nanotechnology, chemistry, surface science, polymer science, electrical engineering, physics, chemical engineering, and materials science. Offers a nanoarchitectonic perspective on emerging fields, such as metal-organic frameworks, porous polymer materials, or biomimetic nanostructures Discusses different approaches to utilizing "soft chemistry" as a source for hierarchically organized materials Offers an interdisciplinary approach to the design and construction of integrated chemical nano systems Discusses novel approaches towards the creation of complex multiscale architectures
Download or read book Protein Lipidation written by Maurine E. Linder and published by Humana. This book was released on 2019-06-01 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.
