Download or read book Archaeal Ribosomes Biogenesis Structure and Function written by Paola Londei and published by Frontiers Media SA. This book was released on 2022-01-24 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Emerging Concepts in Ribosome Structure Biogenesis and Function written by Vijay Kumar and published by Academic Press. This book was released on 2021-09-25 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribosome biogenesis is the process of making ribosomes which are responsible for mRNA translation into proteins. It is a tightly regulated process closely linked to nearly all biochemical and cellular processes, including cell division, growth, and development. Emerging Concepts in Ribosome Structure, Biogenesis, and Function provides a synthesized overview of all the parts engaged in this process. The book begins by providing an introduction to the ribosome factory, its origin, and its evolution of translation. It then goes on to describe ribosome structure including subunits, RNA, and protein components. Ribosome biogenesis and its emergence as a frontier research area for translational potential in cancer and other diseases are also discussed. In addition, the book explores current developments in ribosome research like the emergence of ribosomopathies, how deregulation of ribosome biogenesis can impact disease mechanisms and aging, and the discovery of specialized ribosomes that have specific functions that may translate differentially with consequences on normal and pathological processes. Emerging Concepts in Ribosome Structure, Biogenesis, and Function provides fundamental coverage and emerging research on ribosomes, biogenesis, and their structure and function and is a resourceful introduction for new researchers and those engaged in interdisciplinary ribosomal research. - Provides an overview of ribosome biogenesis and examines its involvement in cell transformation and cancerous growth - Covers disorders related to the ribosome (ribosomopathies) and explains the significance of ribosome dysfunction in human diseases - Includes commonly used methods to study ribosomes, such as polysome preparation, RNA profiling and proteomics, CryoEM, and Cell-free assays along with proper illustrations
Download or read book Ribosomes Structure Function and Dynamics written by Marina V. Rodnina and published by Springer Science & Business Media. This book was released on 2011-12-10 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ribosome is a macromolecular machine that synthesizes proteins with a high degree of speed and accuracy. Our present understanding of its structure, function and dynamics is the result of six decades of research. This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. New high-resolution crystal structures of functional ribosome complexes and cryo-EM structures of translating ribosomes are presented, while partial reactions of translation are examined in structural and mechanistic detail, featuring translocation as a most dynamic process. Mechanisms of initiation, both in bacterial and eukaryotic systems, translation termination, and novel details of the functions of the respective factors are described. Structure and interactions of the nascent peptide within, and emerging from, the ribosomal peptide exit tunnel are addressed in several articles. Structural and single-molecule studies reveal a picture of the ribosome exhibiting the energy landscape of a processive Brownian machine. The collection provides up-to-date reviews which will serve as a source of essential information for years to come.
Download or read book The Ribosome written by Walter Ensign Hill and published by ASM Press. This book was released on 1990 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is recommended as a 'hitchhiker's guide to the ribosome' for everyone with an interest in translation, RNA-protein structures, and macromolecular structure-function relationships Science >.
Download or read book Ribosome Biogenesis written by Karl-Dieter Entian and published by Springer Nature. This book was released on 2022-07-07 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translation was the sine qua non for developing the powerful RNA-based vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in this book are organized into six parts. Part One discusses a comparative survey about the unity and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA transcription. Part Three explores in vitro methods to study RNA polymerase I structure and its function, and Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and RNP complexes. Part Five covers modifications that increase the complexity of rRNAs, and Part Six provides readers with a review of eukaryotic translation and - for the first time - describes a new method to analyze translation in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about the increasing importance of in vitro RNA-technologies.
Download or read book Nuclear Cytoplasmic Transport written by Weidong Yang and published by Springer. This book was released on 2018-07-27 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dysfunction of nuclear-cytoplasmic transport systems has been associated with many human diseases. Thus, understanding of how functional this transport system maintains, or through dysfunction fails to maintain remains the core question in cell biology. In eukaryotic cells, the nuclear envelope (NE) separates the genetic transcription in the nucleus from the translational machinery in the cytoplasm. Thousands of nuclear pore complexes (NPCs) embedded on the NE selectively mediate the bidirectional trafficking of macromolecules such as RNAs and proteins between these two cellular compartments. In this book, the authors integrate recent progress on the structure of NPC and the mechanism of nuclear-cytoplasmic transport system in vitro and in vivo.
Download or read book Protein Synthesis and Ribosome Structure written by Knud H. Nierhaus and published by John Wiley & Sons. This book was released on 2004-10-15 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knud Nierhaus, who has studied the ribosome for more than 30 years, has assembled here the combined efforts of several scientific disciplines into a uniform picture of the largest enzyme complex found in living cells, finally resolving many decades-old questions in molecular biology. In so doing he considers virtually all aspects of ribosome structure and function -- from the molecular mechanism of different ribosomal ribozyme activities to their selective inhibition by antibiotics, from assembly of the core particle to the regulation of ribosome component synthesis. The result is a premier resource for anyone with an interest in ribosomal protein synthesis, whether in the context of molecular biology, biotechnology, pharmacology or molecular medicine.
Download or read book Ribosomes written by Alexander S. Spirin and published by Springer Science & Business Media. This book was released on 1999 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Spirin is a world authority on ribosomes and has published two earlier books in this area in English. This text is for advanced undergraduates and beginning graduate students and will cover the structure, function, and biosynthesis of ribosomes. Ribosomes are important in protein synthesis, which is currently a hot topic in many different areas of research.
Download or read book The Nucleolus and Ribosome Biogenesis written by A.A. Hadjiolov and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 281 pages. Available in PDF, EPUB and Kindle. Book excerpt: The nucleolus had consistently attracted the attention of investigators in the fields of cell biology and pathology. Because of its ubiquitous presence in the nucleus of eukaryotic cells, its rapid changes during their life cycle, and its rapid response to noxious agents, this organelle has been the subject of a large number of studies. Yet, the exact function and the very reason for the existence of the nucleolus (the only large cellular structure not delimited by a membrane) remain largely unknown. The ribosomes were discovered relatively late in the study of cells, but due to their crucial involvement in the protein synthesis machinery of all living organisms, the elucidation of their structure and function quickly became one of the major goals of molecular biology. The relatively simple structure of the ribosome strengthens the hope that a full understanding of the structure and function of this organelle in molecular terms is within the reach of contemporary research~ Since each of the rRNA and protein molecules embodied in the ribosome is the product of a distinct gene, studies on the biogenesis of ribosomes expanded rapidly to become a core topic in molecular genetics.
Download or read book Archaeal Cell Envelope and Surface Structures written by Sonja-Verena Albers and published by Frontiers Media SA. This book was released on 2016-03-10 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Archaea and Bacteria have complex cell envelopes that play important roles in several vital cellular processes, including serving as a barrier that protects the cytoplasm from the environment. Along with associated proteinaceous structures, cell envelopes also ensure cell stability, promote motility, mediate adherence to biotic and abiotic surfaces, and facilitate communication with the extracellular environment. While some aspects of the biosynthesis and structure of the cell are similar to the three domains of life, archaeal cell envelopes exhibit several unique characteristics. Moreover, recent analyzes have revealed that many features of cell envelopes can vary greatly between distantly related archaea. The collection of reviews and original research papers in this focused issue describes research that has been significantly expanded in our understanding of the mechanisms underlying the biogenesis and functions of archaeal cell envelopes and their constituent surface structures. Jain et al. (5) cytoplasmic membrane, isoprenoid lipid bilayer, as well as recently revealed the cytoplasmic membrane biosynthesis, which is conserved across the three domains of life. Complementing this review, Andreas Klingl summarizes the diverse structures and functions of archaeal cytoplasmic membranes (8). While most archaeal cells have a single membrane, the archaea have an outer membrane, which has been thought of in a different variety of archaeal lineages. One particular intriguing diderm is the hyperthermophilic archaeon. In the periplasmic space, ATP in the periplasmic space. Complementing this work, Kletzin provides an in-depth review of evolutionarily conserved and unique archaeal inner and outer membrane-associated cytochromes (7). The periplasmic space between the membranes of archaeal diderms does not contain a peptidoclycan layer. In fact, while the cytoplasmic membrane is superimposed by an S-layer in many monoderm archaea, it is unclear how diderms, and even some monoderm extremophiles that varnish to S-layer, withstand osmotic stress. As noted by Klingl (8), glycocalyx, lipoglycans, or other protective cell-associated glycoproteins, may take on the functions of a cell wall in some archaea. One such secreted protein, as described by Zenke et al., Is the halomucin of Haloquadratum walsbyi (15). While H. walsbyi does not have a cell wall, halomucine, an unusually large protein (9159aa), is thought to play an important role in protecting these extreme halophiles against desiccation. Interestingly, Candidatus Altiarchaeum hamiconexum, an uncultured diderm euryarchaeon, isolated from biofilms containing hammers, cell surface proteins with the appearance of grappling hooks that connect cells to each other and to abiotic surfaces. Perra's stunning imagery suggests that this is the case with the S-layer glycoproteins, possibly suggesting a case of divergent evolution (12). [0003] The present invention relates to a method and apparatus for the preparation of a medical device, Are conserved across the prokaryotic domains, being found in the majority of sequenced archaea, where, as in bacteria, they play key roles in processes necessary for biofilm formation (10, 13). Interestingly, as discussed by Albers and Jarrell (1), as well as Näther et al. (11), a type IV pilus-like structure is responsible for swimming motility in archaea. Many secreted proteins, including the S-layer glycoprotein and pilin-like proteins, are heavily post-translationally modified. [1]. [0002] The known proteolytic modifications of the proteins of the model haloarchaeon [1], vol. Using the results of proteomic studies, Leon et al. (9), providing an invaluable resource in silico prediction tools for the characterization of archaeal proteins, in general, but also specific phyla. Kandiba and Eichler review our current knowledge of N-glycosylation in archaea, including descriptions of the pathways the regulatory roles of this post-translational modification plays in cellular processes (6). Considering the unique aspects of the archaeal cell envelope, including not only the protein structures, but their post-translational modifications as well, it is not surprising that archaeal viruses have evolved specific mechanisms to infect and egress from archaeal cells, which are reviewed in this Issue by Quemin and Quax (14). Understanding the roles that can be seen in this book is a study of the development of biofuels in the field of bioinformatics, including mucosa-associated methanogenic archaea, can (2). (2) In this paper, Archaeal cell membranes and S-layer glycoproteins have been used to make liposomes and nanomaterials. Finally, a better understanding of the similarities and differences among the archaea as well as between the archaea and the other two domains will lead to the development of a more accurate phylogeny. In this issue, Forterre takes advantage of the latest profusion of genome studies, along with supporting in vivo work, to assemble an improved tree of life (3). Conflict of Interest Statement The authors declare that this is not the case. Acknowledgments The support of the National Science Foundation MCB-1413158 to MP and the ERC starting grant 311523 (archaellum) to SA are gratefully acknowledged. References: 1. Albers SV & Jarrell KF (2015) The archaellum: how Archaea swim. Frontiers in microbiology 6:23. 2. Bang C, et al. (2014) Biofilm formation of mucosa-associated methanoarchaeal strains. Frontiers in microbiology 5: 353. 3. Forterre P (2015) The Universal Tree: an update. Frontiers in Microbiology, in 4. Gimenez MI, Cerletti M, & De Castro RE (2015) Archaeal membrane-associated proteases: insights on Haloferax volcanii and other haloarchaea. Frontiers in microbiology 6:39. 5. Jain S, Caforio A, & Driessen AJ (2014) Biosynthesis of archaeal membrane ether lipids. Frontiers in microbiology 5: 641. 6. Kandiba L & Eichler J (2014) Archaeal S-layer glycoproteins: post-translational modification in the face of extremes. Frontiers in microbiology 5: 661. 7. Kletzin A, et al. (2015) Cytochromes c in Archaea: distribution, maturation, cell architecture, and the special case of Ignicoccus hospitalis. Frontiers in microbiology 6: 439. 8. Klingl A (2014) S-layer and cytoplasmic membrane - exceptions from the typical archaeal cell wall with a focus on double membranes. Frontiers in microbiology 5: 624. 9. Leon DR, et al. (2015) Mining proteomic data to expose protein modifications to methanosarcina mazei strain Go1. Frontiers in microbiology 6: 149. 10. Losensky G, Vidakovic L, Klingl A, Pfeifer F, & Frols S (2014) Novel pili-like surface structures of Halobacterium salinarum strain R1 are crucial for surface adhesion. Frontiers in microbiology 5: 755. 11. Nather-Schindler DJ, Schopf S, Bellack A, Rachel R, & Wirth R (2014) Pyrococcus furiosus flagella: biochemical and transcriptional analyzes identify the newly detected flaB0 gene to encode the major flagellin. Frontiers in microbiology 5: 695. 12. Perras AK, et al. (2015) S-layers at second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence. Frontiers in microbiology 6: 543. 13. Pohlschroder M & Esquivel RN (2015) Archaeal type IV pili and their involvement in biofilm formation. Frontiers in microbiology 6:19. 14. Quemin ER & Quax TE (2015) Archaeal viruses at the cell envelope: entry and egress. Frontiers in microbiology 6: 552. 15. Zenke R, et al. (2015) fluorescence microscopy visualization of halomucin, a secreted 927 kDa protein surrounding haloquadratum walsbyi cells. Frontiers in microbiology 6: 249.
Download or read book The Eukaryotic Ribosome written by Heinz Bielka and published by Walter de Gruyter GmbH & Co KG. This book was released on 1982-12-31 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: No detailed description available for "The Eukaryotic Ribosome".
Download or read book Insights Into Synthesis and Function of KsgA Dim1 dependent RRNA Modifications in Archaea written by Robert Knüppel and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Ribosomes are intricate molecular machines ensuring proper protein synthesis in every cell. Ribosome biogenesis is a complex process which has been intensively analyzed in bacteria and eukaryotes. In contrast, our understanding of the in vivo archaeal ribosome biogenesis pathway remains less characterized. Here, we have analyzed the in vivo role of the almost universally conserved ribosomal RNA dimethyltransferase KsgA/Dim1 homolog in archaea. Our study reveals that KsgA/Dim1-dependent 16S rRNA dimethylation is dispensable for the cellular growth of phylogenetically distant archaea. However, proteomics and functional analyses suggest that archaeal KsgA/Dim1 and its rRNA modification activity (i) influence the expression of a subset of proteins and (ii) contribute to archaeal cellular fitness and adaptation. In addition, our study reveals an unexpected KsgA/Dim1-dependent variability of rRNA modifications within the archaeal phylum. Combining structure-based functional studies across evolutionary divergent organisms, we provide evidence on how rRNA structure sequence variability (re-)shapes the KsgA/Dim1-dependent rRNA modification status. Finally, our results suggest an uncoupling between the KsgA/Dim1-dependent rRNA modification completion and its release from the nascent small ribosomal subunit. Collectively, our study provides additional understandings into principles of molecular functional adaptation, and further evolutionary and mechanistic insights into an almost universally conserved step of ribosome synthesis
Download or read book Ribosomes Structure Function and Genetics written by Glenn Chambliss and published by . This book was released on 1980 with total page 1020 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Comprehensive and Molecular Phytopathology written by Yuri Dyakov and published by Elsevier. This book was released on 2007-01-09 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a collection of information on successive steps of molecular 'dialogue' between plants and pathogens. It additionally presents data that reflects intrinsic logic of plant-parasite interactions. New findings discussed include: host and non-host resistance, specific and nonspecific elicitors, elicitors and suppressors, and plant and animal immunity. This book enables the reader to understand how to promote or prevent disease development, and allows them to systematize their own ideas of plant-pathogen interactions.* Offers a more extensive scope of the problem as compared to other books in the market* Presents data to allow consideration of host-parasite relationships in dynamics and reveals interrelations between pathogenicity and resistance factors* Discusses beneficial plant-microbe interactions and practical aspects of molecular investigations of plant-parasite relationships* Compares historical study of common and specific features of plant immunity with animal immunity
Download or read book Structure and Function of the Archaeal Box C D Ribonucleoprotein Complex written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Box C/D ribonucleoprotein complexes (RNPs) are evolutionarily ancient nucleotide modification machines found in both Eukarya and Archaea. The box C/D RNAs are essential for ribosome biogenesis and primarily function by guiding 2'-O-methylation of ribosomal RNA (rRNA). The site of modification is determined by base-pairing between the target RNA and the box C/D RNA through a region of complementarity. The box C/D RNAs possess terminal box C/D and internal C'/D' motifs that fold to form K-turn RNA elements. In eukaryotes, the box C/D RNAs associate with a common set of four core proteins to form an RNP. The core proteins, 15.5kD, Nop56p, Nop58p and Fibrillarin, are differentially distributed on eukaryotic box C/D RNAs to form an asymmetric RNPs. We have characterized the structure and function of the archaeal box C/D RNP using Methanocaldococcus jannaschii sR8 RNP as a model box C/D complex. Archaeal genomes contain genes for a Fibrillarin homolog and a single homolog for both Nop56p and Nop58p termed Nop56/58p. Our initial investigations identified ribosomal protein L7 as the archaeal homolog of the eukaryotic 15.5kD protein. Strikingly, L7 has a dual role as a component of both the ribosome and the box C/D RNP. A methylation-competent sR8 RNP was assembled in vitro using the three recombinant M. jannaschii box C/D RNA core proteins. This reconstituted complex is symmetric with respect to core protein binding and guides nucleotide modification from both the box C/D and C'/D' RNPs. Additionally, efficient RNA 2'-O-methylation requires juxtaposed box C/D and C'/D' motifs on the same box C/D RNP complex. Finally, the identification of box C/D RNPs in both Archaea and Eukarya led us to question the evolutionary origins of these ancient modification complexes. Based on the demonstration of a common RNP element (L7: K-turn motif) in both the archaeal large ribosomal subunit and the box C/D RNP complex, we propose that the trans-acting nucleotide modification machines evolv.
Download or read book The Nucleolus written by Mark O. J. Olson and published by Springer Science & Business Media. This book was released on 2011-09-15 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Within the past two decades, extraordinary new functions for the nucleolus have begun to appear, giving the field a new vitality and generating renewed excitement and interest. These new discoveries include both newly-discovered functions and aspects of its conventional role. The Nucleolus is divided into three parts: nucleolar structure and organization, the role of the nucleolus in ribosome biogenesis, and novel functions of the nucleolus.
Download or read book RNA Based Regulation in Human Health and Disease written by and published by Academic Press. This book was released on 2020-08-19 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA-based Regulation in Human Health and Disease offers an in-depth exploration of RNA mediated genome regulation at different hierarchies. Beginning with multitude of canonical and non-canonical RNA populations, especially noncoding RNA in human physiology and evolution, further sections examine the various classes of RNAs (from small to large noncoding and extracellular RNAs), functional categories of RNA regulation (RNA-binding proteins, alternative splicing, RNA editing, antisense transcripts and RNA G-quadruplexes), dynamic aspects of RNA regulation modulating physiological homeostasis (aging), role of RNA beyond humans, tools and technologies for RNA research (wet lab and computational) and future prospects for RNA-based diagnostics and therapeutics. One of the core strengths of the book includes spectrum of disease-specific chapters from experts in the field highlighting RNA-based regulation in metabolic & neurodegenerative disorders, cancer, inflammatory disease, viral and bacterial infections. We hope the book helps researchers, students and clinicians appreciate the role of RNA-based regulation in genome regulation, aiding the development of useful biomarkers for prognosis, diagnosis, and novel RNA-based therapeutics. Comprehensive information of non-canonical RNA-based genome regulation modulating human health and disease Defines RNA classes with special emphasis on unexplored world of noncoding RNA at different hierarchies Disease specific role of RNA - causal, prognostic, diagnostic and therapeutic Features contributions from leading experts in the field
