Download or read book Aptamers for Analytical Applications written by Yiyang Dong and published by John Wiley & Sons. This book was released on 2019-02-11 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.

Download or read book DNA Nanotechnology for Cell Research written by Zhou Nie and published by John Wiley & Sons. This book was released on 2024-02-13 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Nanotechnology for Cell Research Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery. The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications. Sample topics covered in DNA Nanotechnology for Cell Research include: Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy. Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing. Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation. Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering. Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.

Download or read book Nucleic Acid Biosensors for Environmental Pollution Monitoring written by Marco Mascini and published by Royal Society of Chemistry. This book was released on 2011-05-01 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acids are the fundamental building blocks of life and are found in all living things. In recent years, their functions have been shown to extend beyond the Watson-Crick base pair recognition of complementary strands. Molecules (known as aptamers) consisting of 40-50 nucleotides have been isolated that are able to bind a broad range of molecules with high affinity and specificity. The molecules recognized by aptamers range from small organic molecules to proteins, cells and even intact viral particles. Catalytic DNA molecules called NAzymes (RNAzyme or DNAzyme) have also been shown to exist and, when combined with aptamers, are known as aptazymes. These biomolecules can be used to develop smart and innovative biosensors for environmental analysis. Monitoring of contaminants in the air, water and soil is a key component in understanding and managing risks to human health and ecosystems. This, in conjunction with the time and cost involved in traditional chemical analysis, means there is a growing need for simple, rapid, cost-effective and portable screening methods. Biosensors are compact devices which complement current field screening and monitoring methods. This book demonstrates the incredible opportunities that nucleic acids can offer to environmental analytical chemistry. The chapters: show how nucleic acids have a pivotal role in the development of smart biosensors for environmental monitoring; describe the development of biosensors based on aptamers and NAzymes for the detection of organic and inorganic pollutants; deal with the use of nucleic acid based biosensors for environmental toxicity screening, and detail the use of nanomaterials, as well as miniaturization and lab-on-a-chip technologies, for nucleic acid based biosensing systems.

Download or read book Handbook of Smart Materials in Analytical Chemistry written by Miguel de la Guardia and published by John Wiley & Sons. This book was released on 2019-01-22 with total page 1108 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to smart materials and how they are used in sample preparation, analytical processes, and applications This comprehensive, two-volume handbook provides detailed information on the present state of new materials tailored for selective sample preparation and the legal frame and environmental side effects of the use of smart materials for sample preparation in analytical chemistry, as well as their use in the analytical processes and applications. It covers both methodological and applied analytical aspects, relating to the development and application of new materials for solid-phase extraction (SPE) and solid-phase microextraction (SPME), their use in the different steps and techniques of the analytical process, and their application in specific fields such as water, food, air, pharmaceuticals, clinical sciences and forensics. Every chapter in Handbook of Smart Materials in Analytical Chemistry is written by experts in the field to provide a comprehensive picture of the present state of this key area of analytical sciences and to summarize current applications and research literature in a critical way. Volume 1 covers New Materials for Sample Preparation and Analysis. Volume 2 handles Analytical Processes and Applications. Focuses on the development and applications of smart materials in analytical chemistry Covers both, methodological and applied analytical aspects, for the development of new materials and their use in the different steps and techniques of the analytical process and their application in specific fields Features applications in key areas including water, air, environment, pharma, food, forensic, and clinical Presents the available tools for the use of new materials suitable to aid recognition process to the sample preparation and analysis A key resource for analytical chemists, applied laboratories, and instrument companies Handbook of Smart Materials in Analytical Chemistry, 2V Set is an excellent reference book for specialists and advanced students in the areas of analytical chemistry, including both research and application environments.

Download or read book Nucleic Acid and Peptide Aptamers written by Günter Mayer and published by Humana Press. This book was released on 2014-12-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular BiologyTM series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

Download or read book Aptamers in Bioanalysis written by M. Mascini and published by John Wiley & Sons. This book was released on 2009-04-27 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states. This is a core reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, biotechnologists, and others.

Download or read book Aptamers for Medical Applications written by Yiyang Dong and published by Springer Nature. This book was released on 2021-03-25 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book outlines comprehensively the main medical uses of aptamers, from diagnosis to therapeutics in fourteen chapters. Pioneering topics covered include aptamer pharmaceuticals, aptamers for malign tumors, aptamers for personalized therapeutics and aptamers for point-of-care testing. The book offers an essential guide for medical scientists interested in developing aptamer-based schemes for better theranostics. It is therefore of interest for not only academic researchers, but also practitioners and medical researchers in various fields of medical science, medical research and bio-analytical chemistry.

Download or read book Spherical Nucleic Acids written by Chad A. Mirkin and published by CRC Press. This book was released on 2020-08-31 with total page 1866 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spherical nucleic acids (SNAs) comprise a nanoparticle core and a densely packed and highly oriented nucleic acid shell, typically DNA or RNA. They have novel architecture-dependent properties that distinguish them from all other forms of nucleic acids and make them useful in materials synthesis, catalysis, diagnostics, therapeutics, and optics/plasmonics. This book covers over two decades of Dr. Mirkin’s research on SNAs and their anisotropic analogues, including synthesis and fundamental properties, and applications in colloidal crystallization, adaptive matter, and nanomedicine, spanning extra- and intracellular diagnostics, gene regulation, and immunomodulation. It is a reprint volume that compiles 101 key papers from high-impact journals in this research area published by the Mirkin Group at Northwestern University, Illinois, USA, within the International Institute for Nanotechnology, and collaborators. Volume 1 provides an overview and a historical framework of engineering matter from DNA-modified constructs and discusses the enabling features of nucleic acid–functionalized nanomaterials. Volume 2 covers design rules for colloidal crystallization, building blocks for crystal engineering, and DNA and RNA as programmable bonds. Volume 3 discusses colloidal crystallization processes and routes to hierarchical assembly, dynamic nanoparticle superlattices, surface-based and template-confined colloidal crystallization, optics and plasmonics with nanoparticle superlattices, and postsynthetic modification and catalysis with nanoparticle superlattices. Volume 4 covers diagnostic modalities, and intracellular therapeutic and diagnostic schemes based upon nucleic acid–functionalized nanomaterials.

Download or read book Aptamers Selected by Cell SELEX for Theranostics written by Weihong Tan and published by Springer. This book was released on 2015-03-02 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited volume describes cell-SELEX as the fundamental tool used to generate aptamer molecules for a wide range of applications in molecular medicine, bioanalysis and chemical biology. Easily integrated into the natural heterogeneous cell matrix, aptamers can be effectively used in theranostics, bioanalysis, environment detection and biomedical studies. The book gathers reviews that reflect the latest advances in the field of aptamers and consists in fourteen chapters demonstrating essential examples of these aptamers and aptamer-nanomaterial assemblies, depending on the types of applications and biological systems. It also includes a separate chapter on the utilization of aptamers in real clinics and what will be required to achieve this significant goal. The book will be both appealing and useful to a broad audience, including biologists, bioscientists, and clinicians whose interests range from chemistry and biomedical engineering to cell and molecular biology and biotechnology. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V.T. and Louis Jackson Professor of Chemistry at the University of Florida, USA. Xiaohong Fang is a Professor at the Institute of Chemistry, Chinese Academy of Sciences, China.

Download or read book Molecular Diagnostics Promises and Possibilities written by Mousumi Debnath and published by Springer Science & Business Media. This book was released on 2010-01-29 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.

Download or read book Molecular Beacons written by Chaoyong James Yang and published by Springer Science & Business Media. This book was released on 2013-08-15 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.

Download or read book Aptamers written by Julian Alexander Tanner and published by MDPI. This book was released on 2018-08-27 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue " Aptamers" that was published in IJMS

Download or read book Peptide and Protein Delivery written by Chris Van Der Walle and published by Academic Press. This book was released on 2011-05-12 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growing area of peptide and protein therapeutics research is of paramount importance to medical application and advancement. A needed reference for entry level researchers and researchers working in interdisciplinary / collaborative projects, Peptide and Protein Delivery addresses the current and emerging routes for delivery of therapeutics. Covering cerebral delivery, pulmonary delivery, transdermal delivery, intestinal delivery, ocular delivery, parenteral delivery, and nasal delivery, this resource offers an overview of the main routes in therapeutics. Researchers across biochemistry, pharmaceutical, molecular biology, cell biology, immunology, chemistry and biotechnology fields will find this publication invaluable for peptide and protein laboratory research. - Discusses the most recent data, ideas and concepts - Presents case studies and an industrial perspective - Details information from the molecular level to bioprocessing - Thought provoking, for the novice to the specialist - Timely, for today's biopharmaceuticals market

Download or read book Therapeutic Oligonucleotides written by Jens Kurreck and published by Royal Society of Chemistry. This book was released on 2008 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a compelling overall update on current status of RNA interference

Download or read book Modified Nucleic Acids written by Kazuhiko Nakatani and published by Springer. This book was released on 2016-04-04 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.

Download or read book Aptamers Engineered Nanocarriers for Cancer Therapy written by Prashant Kesharwani and published by Woodhead Publishing. This book was released on 2022-09-25 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aptamers Engineered Nanocarriers for Cancer Therapy details the selection technologies, biological characteristics, and clinical uses of aptamer-based nano agents for cancer therapeutics. The book helps facilitate speedy solutions for some of the problems pertaining to the manufacture of nano-aptamers – such as toxicity, thermal stability, cost efficiency, tumor penetration and blood stability. Key chapters cover cell-SELEX technology for aptamer selection, mechanisms of multi-drug resistance of cancer, the relevance of aptamers as anticancer therapies, as well as the broad range of aptamer-functionalized nanostructures available. This book provides exciting insights into this relatively new approach to cancer therapeutics, and will be of interest to materials scientists, biomedical engineers, molecular biologists, biochemists and clinical scientists, with a focus on cancer therapy. - Reviews the mechanisms behind multi-drug resistance (MDR) in cancer and how aptamer-mediated novel therapeutic agents and strategies can facilitate MDR reversal - Covers a range of aptamers engineered nanostructures, including PLGA nanoparticles, silica nanoparticles, quantum dots, nucleic acid aptamers, and more - Discusses the challenges associated with using aptamers as cancer therapeutics and how this translates into clinical use

Download or read book The Aptamer Handbook written by Sven Klussmann and published by John Wiley & Sons. This book was released on 2006-08-21 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: In The Aptamer Handbook, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.