Download or read book Application of Laser Induced Fluorescence to the Spectroscopy and Kinetics of Simple Molecules written by I. S. McDermid and published by . This book was released on 1977 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Study of Molecular Kinetics and Dynamics Using Laser Induced Fluorescence written by and published by . This book was released on 1984 with total page 1022 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laser induced Fluorescence Spectroscopy of Rb Atoms and Hg 2 Molecules microform written by Jan Szczęsny Supronowicz and published by National Library of Canada. This book was released on 1985 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Modern Fluorescence Spectroscopy written by E. L. Wehry and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the appearance of the first two volumes of Modern Fluorescence Spectroscopy in 1976, important advances continue to be made in both the techniques and applications of molecular luminescence. In terms of "hardware," it is only recently that the application of laser excitation to molecular fluorometry has become feasible under conditions that are analy tically realistic. The improvements that can be effected in sensitivity, analy tical selectivity, and ability to handle "difficult" samples by laser fluorometry have only begun to be exploited. Likewise, time-resolved fluorometry has received widespread use in fundamental studies (a sizable number of which deal with biological systems), but has as of yet received relatively little analytical utilization. The use of electronic array detectors offers the promise of obtaining luminescence spectra more rapidly, and perhaps ultimately with greater sensitivity, than is possible by the use of scanning instruments equipped with conventional detectors. The increasing capabilities of microcomputers and the increasing sophistication of "smart" spectroscopic instrumentation signify that much more efficient acquisition and use can now be achieved of the information contained in the "excitation-emission matrix" inherent in the luminescence phenomenon.
Download or read book Principles and Applications of Fluorescence Spectroscopy written by Jihad Rene Albani and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.
Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.
Download or read book The Application of Laser Induced Fluorescence to the Study of Electron Impact Excitation of Atoms and Molecules microform written by Peter W. Zetner and published by National Library of Canada. This book was released on 1985 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kinetics and Magnetic Field Effects on Laser Induced Fluorescence in NO2 written by Gary Raymond Gust and published by . This book was released on 1978 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical and Biochemical Applications of Lasers V5 written by C. Bradley Moore and published by Elsevier. This book was released on 2012-12-02 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical and Biological Applications of Lasers, Volume V focuses on the laser applications in photochemistry and spectroscopy. This book examines the spectroscopic detection of single atoms and explores the purification of industrial chemicals. Organized into seven chapters, this volume starts with an overview of the methods developed for laser detection of single atoms, including fluorescence, photoionization, photodeflection, and their combinations. This text then discusses the methods of high resolution spectroscopy, which provide detailed information on molecular structure and reaction kinetics studies. Other chapters review several laser photodissociation studies, which explain dissociation dynamics. This book discusses as well the possibilities for selective photochemical reactions and examines the potential of lasers for practical application in chemical processing. The final chapter considers the various metals that can undergo a photochemical change in oxidation state in ordinary solvents. This book is a valuable resource for physicists, chemists, electrochemists, photochemists, electrical engineers, and chemical engineers.
Download or read book Applied Fluorescence in Chemistry Biology and Medicine written by Wolfgang Rettig and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: This interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering. Written for practitioners and researchers in industry and academia, it covers fields like environmental and materials science, biology, medicine, physics and chemistry. Moreover, it reports on such new and breathtaking methods as ultra-fast time-resolved or single molecule spectroscopy, gives examples of applications in the fields of electroluminescent polymers, visualization of membrane potentials in neurons and fluorescence imaging of the brain.
Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Download or read book Laser Induced Fluorescence of Trapped Molecular Ions written by and published by . This book was released on 1980 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser induced fluoresence (LIF) spectra (laser excitation spectra) are conceptually among the most simple spectra to obtain. One need only confine a gaseous sample in a suitable container, direct a laser along one axis of the container, and monitor the sample's fluorescence at a right angle to the laser beam. As the laser wavelength is changed, the changes in fluorescence intensity map the absorption spectrum of the sample. (More precisely, only absorption to states which have a significant radiative decay component are monitored.) For ion spectroscopy, one could benefit in many ways by such an experiment. Most optical ion spectra have been observed by emission techniques, and, aside from the problems of spectral analysis, discharge emission methods often produce the spectra of many species, some of which may be unknown or uncertain. Implicit in the description of LIF given above is certainty as to the chemical identity of the carrier of the spectrum. This article describes a method by which the simplifying aspects of LIF can be extended to molecular ions (albeit with a considerable increase in experimental complexity over that necessary for stable neutral molecules).
Download or read book Index to Theses Accepted for Higher Degrees by the Universities of Great Britain and Ireland and the Council for National Academic Awards written by and published by . This book was released on 1977 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Fluorescence written by Bernard Valeur and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
Download or read book Laser Induced Fluorescence Spectroscopy for Applications in Chemical Sensing and Optical Refrigeration written by Eric Barimah (Kumi) and published by . This book was released on 2014 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Low Temperature Laser induced Fluorescence Studies of Chromophores in Soft Solids and Biological Matter written by Chen Lin and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Low-temperature laser-induced fluorescence spectroscopy has various applications in analytical, physical, and biophysical chemistry. This technique provides information on the fluorescence origin band, zero-phonon lines and phonon-sidebands, inhomogeneous broadening, electron-phonon coupling strength, and ground- and excited-state vibrational frequencies of studied molecules. Examples discussed in this work include studies of DNA/metabolites and monoclonal antibody (mAb)/antigen interactions. The structural basis for the increased reactivity of BPDE towards guanines at 5-methylcytosine ([superscript]M[superscripte]eC):G sites in DNA was investigated by low temperature laser-based spectroscopy, studying the nature of physical complexes of benzo[a]pyrene tetraol in a series of 5-methylcytosine structural DNA analogs. We found that the presence of a C-5 substituent on cytosine and related structural modifications influences the conformation of BPT in DNA analogs, and could explain the increase in guanine reactivity at [superscript]M[superscript]eC:G sites of the p53 tumor suppressor gene that contains endogenenous 5-([superscript]M[superscript]eC. It has been demonstrated that various mAbs can bind a particular cross-reactant by adopting two distinct "red" and "blue" conformations of its binding sites. We showed that the blue conformation of pyrene in several mAbs (including 4D5 mAb) is consistent with [pi]-cation interactions, underscoring the importance of [pi]-cation interaction in ligand binding. We propose that considerable narrowing of the fluorescence origin band of the ligand in the protein environment could be regarded as a simple indicator of [pi]-cation interactions. It is also shown that time-resolved delta fluorescence line-narrowing ([delta]FLN) spectroscopy, using excitation within the (0,0)-transition band, provides more reliable information of the frequency dependence of the electron-phonon coupling (Huang-Rhys factor, (S
Download or read book Rapid Screening and Isolation of Single Molecules by Laser induced Fluorescence Spectroscopy in Microdroplets written by Katie Hua Heredia and published by . This book was released on 2008 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
