Download or read book Clinical Trials of Genetic Therapy with Antisense DNA and DNA Vectors written by Eric Wickstrom and published by CRC Press. This book was released on 2020-08-13 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: An important new collection of clinical and preclinical reports on genetic therapy, this book describes illustrative examples of diseases in which gene-based interventions are presently plausible, and presents case studies of current research using both synthetic oligonucleotides and biological vectors. Combining the insights of over 50 contributors, Clinical Trials of Genetic Therapy with Antisense DNA and DNA Vectors furnishes a historical overview of genetic therapy highlights official Food and Drug Administration positions on the preparation of oligonucleotides and vectors offers practical models of agent preparation, animal testing, pharmacokinetics, toxicology, and clinical trials discusses both synthetic DNA and biological vector approaches to cancer, viral, and cardiological indications illustrates for new practitioners how each stage of genetic therapy is developed details genetic treatment of leukemia; lymphoma; cancer of the brain, breast, colon, kidney, and lung; melanoma; HIV; and coronary restenosis includes examples of antisense, ribozyme, tumor suppressor, immunostimulation, and gene replacement therapy and addresses questions of preparation, delivery, toxicity, mechanism, and specificity.
Download or read book Antisense Elements genetics Research Focus written by A. G. Hernandes and published by Nova Publishers. This book was released on 2007 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antisense molecules interact with complementary strands of nucleic acids, modifying expression of genes. Some regions within a double strand of DNA code for genes, which are usually instructions specifying the order of amino acids in a protein along with regulatory sequences, splicing sites, non-coding introns and other complicating details. For a cell to use this information, one strand of the DNA serves as a template for the synthesis of a complementary strand of RNA. The template DNA strand is called the transcribed strand with antisense sequence and the mRNA transcript is said to be sense sequence (the complement of antisense). Because the DNA is double-stranded, the strand complementary to the antisense sequence is called non-transcribed strand and has the same sense sequence as the mRNA transcript (though T bases in DNA are substituted with U bases in RNA). This book presents important new research from around the world in this field.
Download or read book Antisense RNA and DNA written by Douglas A. Melton and published by . This book was released on 1988 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Antisense Technology written by Conrad Lichtenstein and published by IRL Press. This book was released on 1997 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologies are then described, with chapters on antisense techniques in IDictyostelium, plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo. Finally, non-antisense effects of oligonucleotides, "anti-sense rescue", shotgun antisense mutagenesis, and in vivo, in vitro and in situ detection of sense: antisense duplexes by structure-specific anti-RNA antibodies are discussed. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, or protein function.
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Download or read book Antisense RNA and DNA written by James A. H. Murray and published by Wiley. This book was released on 1992-07-30 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a compilation of authoritative short reviews regarding the development and application of antisense nucleic acids capable of both regulating and manipulating gene expression in vitro or in vivo. Features novel experimental approaches and findings using model systems ranging from prokaryotes to higher eukaryotes.
Download or read book Research Progress in Antisense Elements genetics written by Jessica C. Taylor and published by . This book was released on 2008 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents important research from around the world in the field of Antisense molecules which interact with complementary strands of nucleic acids, modifying expression of genes. Some regions within a double strand of DNA code for genes, which are usually instructions specifying the order of amino acids in a protein along with regulatory sequences, splicing sites, non-coding introns and other complicating details. For a cell to use this information, one strand of the DNA serves as a template for the synthesis of a complementary strand of RNA. The template DNA strand is called the transcribed strand with antisense sequence and the mRNA transcript is said to be sense sequence (the complement of antisense). Because the DNA is double-stranded, the strand complementary to the antisense sequence is called non-transcribed strand and has the same sense sequence as the mRNA transcript (though T bases in DNA are substituted with U bases in RNA). Many forms of antisense have been developed and can be broadly categorised into enzyme-dependent antisense or steric blocking antisense.
Download or read book Antisense and Ribozyme Methodology written by Ian Gibson and published by John Wiley & Sons. This book was released on 2008-09-26 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating viral infections and cancer. This laboratory companion provides detailed information on the potential, advantages and limitations of this methodology. It critically discusses potential pitfalls, presents strategies for choosing targets and delivery systems, so as to allow the selection of the optimum methodology for achieving fast and reliable experimental success with any human or other biological system. For researchers, technicians and advanced graduates in experimental medicine, molecular and cell biology.
Download or read book Gene Regulation written by Robert P. Erickson and published by Lippincott Williams & Wilkins. This book was released on 1992 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examines the biology of antisense oligodeoxynucleotides, RNA and ribozymes, and explores their potential therapeutic applications, particularly for inhibition of viral replication and cancer gene expression. Close attention is also given to drug approval.
Download or read book Antisense Nucleic Acids and Proteins written by Joseph N. M. Mol and published by CRC Press. This book was released on 1991-01-24 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: A compilation of papers from many different scientific meetings in recent years, this reference source reviews the use of antisense nucleic acids to modulate or down-regulate gene expression, provides clues on the action mechanism of sense and antisense genes as well as data on antisense nucleic aci
Download or read book Antisense Strategies written by Renato Baserga and published by . This book was released on 1992 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: The antisense strategy has been used to study cellular proliferation and differentiation as well as to target chemotherapy against viral products such as HIV. It shows promise in the isolation of bone marrow stem cells and the purging of bone marrow. This volume, covering cellular proliferation, inhibition of viral replication, ribozymes-transcriptural regulation, cellular differentiation, and transgenic animals should be important to both basic and clinical scientists.
Download or read book Oligonucleotide Based Drugs and Therapeutics written by Nicolay Ferrari and published by John Wiley & Sons. This book was released on 2018-07-31 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive review of contemporary antisense oligonucleotides drugs and therapeutic principles, methods, applications, and research Oligonucleotide-based drugs, in particular antisense oligonucleotides, are part of a growing number of pharmaceutical and biotech programs progressing to treat a wide range of indications including cancer, cardiovascular, neurodegenerative, neuromuscular, and respiratory diseases, as well as other severe and rare diseases. Reviewing fundamentals and offering guidelines for drug discovery and development, this book is a practical guide covering all key aspects of this increasingly popular area of pharmacology and biotech and pharma research, from the basic science behind antisense oligonucleotides chemistry, toxicology, manufacturing, to safety assessments, the design of therapeutic protocols, to clinical experience. Antisense oligonucleotides are single strands of DNA or RNA that are complementary to a chosen sequence. While the idea of antisense oligonucleotides to target single genes dates back to the 1970's, most advances have taken place in recent years. The increasing number of antisense oligonucleotide programs in clinical development is a testament to the progress and understanding of pharmacologic, pharmacokinetic, and toxicologic properties as well as improvement in the delivery of oligonucleotides. This valuable book reviews the fundamentals of oligonucleotides, with a focus on antisense oligonucleotide drugs, and reports on the latest research underway worldwide. • Helps readers understand antisense molecules and their targets, biochemistry, and toxicity mechanisms, roles in disease, and applications for safety and therapeutics • Examines the principles, practices, and tools for scientists in both pre-clinical and clinical settings and how to apply them to antisense oligonucleotides • Provides guidelines for scientists in drug design and discovery to help improve efficiency, assessment, and the success of drug candidates • Includes interdisciplinary perspectives, from academia, industry, regulatory and from the fields of pharmacology, toxicology, biology, and medicinal chemistry Oligonucleotide-Based Drugs and Therapeutics belongs on the reference shelves of chemists, pharmaceutical scientists, chemical biologists, toxicologists and other scientists working in the pharmaceutical and biotechnology industries. It will also be a valuable resource for regulatory specialists and safety assessment professionals and an important reference for academic researchers and post-graduates interested in therapeutics, antisense therapy, and oligonucleotides.
Download or read book Antisense Drug Technology written by Stanley T. Crooke and published by CRC Press. This book was released on 2007-07-25 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research. Interpreting lessons learned from the clinical trials of first generati
Download or read book Delivery Strategies for Antisense Oligonucleotide Therapeutics written by Saghir Akhtar and published by CRC Press. This book was released on 2017-07-28 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions from leading experts, this book is the first to focus solely on addressing the problems and reviewing the strategies currently being used to improve the delivery of antisense nucleic acids. Important delivery issues, such as improving biological stability, improving cell-specific targeting and cellular uptake, manipulating subcellular distribution and producing liposomal delivery systems for antisense agents are comprehensively covered in this volume. This book links review-type articles with contributions that contain exciting never-before-published data on the cellular delivery of oligonucleotides. It stimulates reading for both established researchers and newcomers to the antisense field.
Download or read book Antisense RNA and DNA written by Harold M. Weintraub and published by . This book was released on 1990 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning written by Margaret M. McCarthy and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: When first introduced, antisense oligonucleotides were hailed as the long awaited magic bullet that would provide an unprecedented level of specificity in controlling gene expression. Following this initial enthusiasm, antisense oligonucleotides have been maligned as nonspecific, toxic, and essentially useless. However, application of antisense oligonucleotide technology in the nervous system stands apart from the use of this technique in peripheral systems, largely because of its enormous success. The source of this success remains a matter of some controversy. Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning addresses the origins of that controversy and determines whether the nervous system is a privileged site for antisense oligonucleotide action and not subject to the same vagaries and pitfalls as non-neuronal systems. Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning contains chapters by experts in the field that focus on the use of this technique in a variety of behavioral systems, as well as rapid and nonspecific effects and the uptake and metabolism of antisense oligonucleotides by the nervous system. Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning features: experts in the field reporting on the use of antisense oligonucleotide technology in a variety of behavioral systems, including pain control, circadian rhythms, ingestion and control of water balance, and reproductive behaviors; the novel use of plasmids to express antisense RNA in the nervous system; the biodistribution and metabolism of antisense oligonucleotides in the nervous system; rapid and unusual effects; and non-specific effects.
Download or read book Discoveries in Antisense Nucleic Acids written by Christine L. Brakel and published by . This book was released on 1989 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
