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Book IN VITRO EFFECT OF SODIUM HYPOCHLORITE ON STREPTOCOCCUS MUTANS MONO SPECIES BIOFILM

Download or read book IN VITRO EFFECT OF SODIUM HYPOCHLORITE ON STREPTOCOCCUS MUTANS MONO SPECIES BIOFILM written by Shikhah Alajmi and published by . This book was released on 2017 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt: Objectives: Bacterial biofilms are ubiquitous, found in a variety of sites within the human body, and are associated with the pathogenesis of dental caries. They are formed when free-floating microorganisms attach to a surface. One such organism, Streptococcus mutans, has adhesins allowing its attachment to tooth surfaces. S. mutans is associated with all forms of human dental caries. S. mutans can rapidly metabolize dietary sugars to acid, locally creating a low pH on tooth surfaces, where it can optimally grow and become more competitive in dental plaque biofilms, in contrast to acid-sensitive bacterial species associated with sound non-carious tooth surfaces. Commensal and natural biofilm aggregates contain multiple microbial species that are believed to co-exist, interact, and form families with high bacteria and niche diversity. In contrast, most biofilms that are chronically infectious tend to have low bacterial diversity with sovereign mono-species such as S. mutans. Hence, it is advantageous to study mono-species biofilms of dental caries-associated bacteria. Although dental biofilms cannot be completely eliminated, their pathogenicity can be lessened through effective oral hygiene measures. Continuous and regular disruption of dental biofilms is imperative for prevention and management of oral infectious diseases. Mechanical methods, most notably tooth brushing and flossing, are required to regularly and effectively disrupt dental plaque biofilms in the human oral cavity. Antiseptics, such as mouth rinses, can also help control dental plaque biofilms, and may gain access to oral sites inaccessible by mechanical methods. Among available antiseptics, sodium hypochlorite is a particularly potent agent against bacteria, fungi, and viruses. Sodium hypochlorite occurs naturally within phagocytic cells (neutrophils, monocytes, and macrophages) participating in the human innate immune response to microbial infection. Several randomized controlled clinical trials support the efficacy of rinsing twice weekly with 0.25 % sodium hypochlorite to improve periodontal health. To date, no studies have been reported concerning the effect of sodium hypochlorite rinsing on dental caries incidence or progression. Several in vitro studies have addressed the effect of dilute sodium hypochlorite on various oral bacteria species. However, there are no literature reports addressing the effect of sodium hypochlorite on bacteria attached to solid surfaces in biofilms. Hence, the objective of this study is to compare the in vitro antimicrobial effect of sodium hypochlorite to another antiseptic agent, chlorhexidine, on the acidigenic and aciduric bacterial species, S. mutans and Lactobacillus casei, both in a free-form (planktonic) state, and as a biofilm attached to two hard surfaces (glass and hydroxyapatite). Methods: The effect of sodium hypochlorite on S. mutans and L. casei was determined by finding the minimum inhibitory concentration (MIC) by broth dilution assays. The MIC was considered to be the lowest concentration of the agent that prevented bacterial growth, resulting in a clear test tube in a broth dilution assay. Experiments with sodium hypochlorite were repeated twice against each bacterial species, and compared to chlorhexidine in similar assays. The effect of a 20-second exposure of MIC concentration (1.0%) of sodium hypochlorite on the growth of S. mutans planktonic cells was measured. Finally, the effect of a 20-second exposure of 1% hypochlorite on S. mutans single-species biofilms was assayed using sterile microplates and the MBEC Biofilm Inoculator (Innovatech). Results: Values for inhibition of growth of planktonic cells were as follows: sodium hypochlorite (MIC = 0.1%; MBC = 0.1%); chlorhexidine (MIC = 0.0015%; MBC = 0.0025%). A 20-second exposure to either solution at the MBC inhibited growth of planktonic cells in refreshed media. Cells adherent to glass or hydroxyapatite pegs were growth-inhibited, but not detached, in refreshed media by a 20-second exposure to 0.2% and 0.4% sodium hypochlorite, and 0.01% and 0.12 % chlorhexidine, but not by more dilute solutions. Conclusions: Chlorhexidine is more potent than sodium hypochlorite, but dilute solutions of both antimicrobial agents inhibited growth of S. mutans mono-species biofilm cells without detachment of cells.

Book Natural Oral Care in Dental Therapy

Download or read book Natural Oral Care in Dental Therapy written by Durgesh Nandini Chauhan and published by John Wiley & Sons. This book was released on 2020-04-14 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because of increasing antibiotic resistance, stronger antibiotics are reserved for serious active infection, paving the way for a greater use of herbal antibiotics. This book helps dentists in implementing safe and effective natural medicine therapies to complement the current practice guidelines. Oral diseases continue to be a major health problem world-wide. Oral health is integral to general well-being and relates to the quality-of-life that extends beyond the functions of the craniofacial complex. The standard Western medicine has had only limited success in the prevention of periodontal disease and in the treatment of a variety of oral diseases. The dentist needs to be more informed regarding the use, safety and effectiveness of the various traditional medicines and over-the-counter products. Herbal extracts have been used in dentistry for reducing inflammation, as antimicrobial plaque agents, for preventing release of histamine and as antiseptics, antioxidants, antimicrobials, antifungals, antibacterials, antivirals and analgesics. They also aid in healing and are effective in controlling microbial plaque in gingivitis and periodontitis and thereby improving immunity. The 26 chapters in this unique book explore all the measures to utilize the natural oral care obtained from plants, animals and mineral drugs for dental care.

Book Essential Oil Research

    Book Details:
  • Author : Sonia Malik
  • Publisher : Springer
  • Release : 2019-06-07
  • ISBN : 3030165469
  • Pages : 449 pages

Download or read book Essential Oil Research written by Sonia Malik and published by Springer. This book was released on 2019-06-07 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the advances in essential oil research, from the plant physiology perspective to large-scale production, including bioanalytical methods and industrial applications. The book is divided into 4 sections. The first one is focused on essential oil composition and why plants produce these compounds that have been used by humans since ancient times. Part 2 presents an update on the use of essential oils in various areas, including food and pharma industries as well as agriculture. In part 3 readers will find new trends in bioanalytical methods. Lastly, part 4 presents a number of approaches to increase essential oil production, such as in vitro and hairy root culture, metabolic engineering and biotechnology. Altogether, this volume offers a comprehensive look at what researchers have been doing over the last years to better understand these compounds and how to explore them for the benefit of the society.

Book The Microbiota in Gastrointestinal Pathophysiology

Download or read book The Microbiota in Gastrointestinal Pathophysiology written by Martin H. Floch and published by Academic Press. This book was released on 2016-11-16 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Microbiota in Gastrointestinal Pathophysiology: Implications for Human Health, Prebiotics, Probiotics and Dysbiosis is a one-stop reference on the state-of-the-art research on gut microbial ecology in relation to human disease. This important resource starts with an overview of the normal microbiota of the gastrointestinal tract, including the esophagus, stomach, Ileum, and colon. The book then identifies what a healthy vs. unhealthy microbial community looks like, including methods of identification. Also included is insight into which features and contributions the microbiota make that are essential and useful to host physiology, as is information on how to promote appropriate mutualisms and prevent undesirable dysbioses. Through the power of synthesizing what is known by experienced researchers in the field, current gaps are closed, raising understanding of the role of the microbiome and allowing for further research. Explains how to modify the gut microbiota and how the current strategies used to do this produce their effects Explores the gut microbiota as a therapeutic target Provides the synthesis of existing data from both mainstream and non-mainstream sources through experienced researchers in the field Serves as a ‘one-stop’ shop for a topic that’s currently spread across a number of various journals

Book Single Cell Analysis

    Book Details:
  • Author : Tuhin Subhra Santra
  • Publisher : MDPI
  • Release : 2021-06-02
  • ISBN : 3036506284
  • Pages : 254 pages

Download or read book Single Cell Analysis written by Tuhin Subhra Santra and published by MDPI. This book was released on 2021-06-02 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.

Book Advances in Probiotics for Sustainable Food and Medicine

Download or read book Advances in Probiotics for Sustainable Food and Medicine written by Gunjan Goel and published by Springer Nature. This book was released on 2020-11-25 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on probiotics as sustainable foods and medicines, discussing issues such as screening and identification of probiotics, health claims, and advances in processing technologies, as well as food safety. Based on sound scientific research, the book is a unique reference resource for food scientists interested in development of probiotic based functional foods and their marketing. It will also appeal to those working in the area of regulations regarding the use of and health claims for fermented foods, both locally and globally.

Book Prokaryotic Antimicrobial Peptides

Download or read book Prokaryotic Antimicrobial Peptides written by Djamel Drider and published by Springer Science & Business Media. This book was released on 2011-03-08 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book will provide an overview of the advancement of fundamental knowledge and applications of antimicrobial peptides in biomedical, agricultural, veterinary, food, and cosmetic products. Antimicrobial peptides stand as potentially great alternatives to current antibiotics, and most research in this newly-created area has been published in journals and other periodicals. It is the editors’ opinion that it is timely to sum up the most important achievements in the field and provide the scientific community in a reference book. The goals of this project include illustrating the achievements made so far, debating the state of the art, and drawing new perspectives.

Book Beneficial Microorganisms in Medical and Health Applications

Download or read book Beneficial Microorganisms in Medical and Health Applications written by Min-Tze Liong and published by Springer. This book was released on 2015-12-12 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is devoted to the application of microorganisms in medical treatment and health protection. Topics discussed include the role of probiotics in immune modulation, in prevention of influenza, and in atopic dermatitis. Further chapters cover aspects such as the relation of the gut microbiome and stress, the immune system, the regulation of inflammation, the benefits of Bifidobacterium for infants, and bacteriocin in medical applications, as well as the use of in vitro models of the gastrointestinal tract, omics approaches for targeting microbial health potential and the production of hepatitis B vaccines. This volume will be of particular interest to scientists working in the fields of clinical medicine, applied microbiology, pharmacy and public health.

Book Wound Healing Research

    Book Details:
  • Author : Prasun Kumar
  • Publisher : Springer Nature
  • Release : 2021-07-20
  • ISBN : 9811626774
  • Pages : 694 pages

Download or read book Wound Healing Research written by Prasun Kumar and published by Springer Nature. This book was released on 2021-07-20 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest knowledge on both the physiological and the microbiological aspects of wound healing. Fresh insights into the process of cutaneous wound healing are described, which involves tissue regeneration and repair processes consisting of a sequence of molecular and cellular events. The management of infected wounds is then discussed in detail, covering the roles of traditional medicine practices, novel anti-infective formulations, non-antibiotic approaches, and probiotic bacteria. A section devoted to the interdisciplinary approach to wound care addresses topics including in vitro and in vivo research models, the development of advanced wound dressings, tissue engineering, and the potential applications of bioscaffolds. The authors are all leading researchers in the field. This book is an attempt to showcase current research status and future directions in the area of wound-healing research, which must be of interest to a large group of readers and researchers interested in this field.

Book Probiotics in Food Safety and Human Health

Download or read book Probiotics in Food Safety and Human Health written by Ipek Goktepe and published by CRC Press. This book was released on 2005-10-10 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of new and previously unknown organisms that cause foodborne illness makes it essential for scientists, regulators, and those in the food industry to reconsider their traditional approaches to food preservation. A single source reference that can provide the latest practical information on how to deal with the range of probiotic healt

Book Pouchitis and Ileal Pouch Disorders

Download or read book Pouchitis and Ileal Pouch Disorders written by Bo Shen and published by Academic Press. This book was released on 2018-11-05 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pouchitis and Ileal Pouch Disorders: A Multidisciplinary Approach for Diagnosis and Management provides much needed information on the evolution of pouch surgery, pouch surgery techniques, and surgery-associated complications, including inflammatory, functional, neoplastic, and metabolic complications. The book provides information on the anatomy of the pouch, pathogenesis of pouchitis and other pouch disorders, proper diagnostic modalities, and medical, endoscopic and surgical options for those disorders. The information has been compiled from a panel of national and international leading experts in the field, including basic scientists, gastrointestinal (GI) pathologists, GI radiologists, gastroenterologists, and more. Features never-before-published information and technology from the vast experience of the contributors and editors in diagnosis and medical, endoscopic, and surgical management of pouchitis and other pouch disorders Contains easy to access recommendations from experts Provides access to an accompanying website with videos of endoscopic demonstrations of various configurations of the pouch, endoscopic evaluation of pouch disorders, and endoscopic treatment of pouch strictures, fistula, and anastomotic leaks/sinuses

Book Soybean and Health

    Book Details:
  • Author : Hany El-Shemy
  • Publisher :
  • Release : 2011
  • ISBN : 9789535151715
  • Pages : 514 pages

Download or read book Soybean and Health written by Hany El-Shemy and published by . This book was released on 2011 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Worldwide, soybean seed proteins represent a major source of amino acids for human and animal nutrition. Soybean seeds are an important and economical source of protein in the diet of many developed and developing countries. Soy is a complete protein, and soy-foods are rich in vitamins and minerals. Soybean protein provides all the essential amino acids in the amounts needed for human health. Recent research suggests that soy may also lower risk of prostate, colon and breast cancers as well as osteoporosis and other bone health problems, and alleviate hot flashes associated with menopause. This volume is expected to be useful for student, researchers and public who are interested in soybean.

Book Dental Caries

    Book Details:
  • Author : Ole Fejerskov
  • Publisher : John Wiley & Sons
  • Release : 2009-03-16
  • ISBN : 1444309285
  • Pages : 641 pages

Download or read book Dental Caries written by Ole Fejerskov and published by John Wiley & Sons. This book was released on 2009-03-16 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Dental Caries: the Disease and its Clinical Management builds on the success of the prestigious first edition to present an unrivaled resource on cariology. The clinical thrust of the first edition is widened and strengthened to include coverage of the disease in all its variety, from eruption of the first primary tooth to the prevalent forms of the disease in older patients. The centrality of caries control and management to the dental health of all populations is further emphasized, as the book goes beyond the successful treatment of carious lesions to demonstrate the long-term consequences of the non-operative and therapeutic techniques employed.

Book Bacterial Diversity and Systematics

Download or read book Bacterial Diversity and Systematics written by F.G. Priest and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial taxonomy as a specialized discipline is practised by a minority but the applications of taxonomy are important to most, if not all microbiologists. It is the implementation of taxonomic ideas and practises which gives rise to identification and typing systems, procedures for the analysis and characterization of biodiversity, hypotheses about the evolution of micro-organisms, and improved procedures for the isolation and implementation of bacteria in biotechnological processes. Without taxonomic theory providing a sound basis to these many facets of microbiology there would be severe problems faced by many scientists working with micro-organisms. Taxonomy comprises three sequential but independent processes; classification, nomenclature and identification. The first two stages are the prime concern of the specialist taxonomist but the third stage should result in identification schemes of value to all microbiologists. As the classification and identification of micro-organisms improves, largely due to the introduction of new technologies, so does its contribution to the subject as a whole. It therefore seemed timely to hold a conference in the autumn of 1993 devoted to microbial identification. Such a topic could not be addressed without some reference to the enabling discipline of classification, but the principal aims were to assess improvements in identification and typing and how these were benefiting microbiological topics ranging from ecological and biotechnological studies of extremophilic bacteria to the use of pyrolysis mass spectrometry in epidemiology. The meeting, which was held in Granada, Spain, was supported by FEMS (FEMS Symposium No.