Download or read book Culture of Animal Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 1993-12-29 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Download or read book Animal Cells Culture and Media written by D. C. Darling and published by Wiley-Blackwell. This book was released on 1994 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference guide covers the fundamentals of animal cell cultures. It includes details of culture media, supplements, culture vessels, standard cell lines, passaging, cell separation techniques, cryopreservation, transfection, cell cloning and the creation of cell lines.

Download or read book Animal Cell Culture written by John M. Davis and published by John Wiley & Sons. This book was released on 2011-03-16 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Download or read book Animal Cell Biotechnology written by Ralf Pörtner and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Download or read book Animal Cell Culture and Technology written by Michael Butler and published by Garland Science. This book was released on 2004-08-02 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Download or read book Animal Cell Culture Techniques written by Martin Clynes and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Download or read book Animal Cell Culture written by Mell Carter & and published by Scientific e-Resources. This book was released on 2019-10-15 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favourable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established. Stem cells retain the capacity to self renew as well as to produce progeny with a restricted mitotic potential and restricted range of distinct types of differentiated cell they give rise to. The formation of blood cells, also called haematopoiesis, is the classical example of concept of stem cells. Animal cell and tissue culture is an integral part of biotechnology and this book covers all the aspects of animal cell culture. Animal cells are used for making new vaccines, specific animal proteins such as intergerons, blood factors and hormones, monoclonal antibodies for use as diagnostic and therapeutics, gene probes as diagnostic too, enzymes and last but not the least many new and important compounds. This book contains eleven Chapters, which deal with historic developments, laboratory design, sterilization procedures and various facets of animal cell culture. This includes preservation, characterizations, storage and transport of cells, their monitoring and technologies for cell banking.

Download or read book Medicines from Animal Cell Culture written by Glyn N. Stacey and published by John Wiley & Sons. This book was released on 2007-06-29 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicines from Animal Cell Culture focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells. Medicines from Animal Cell Culture: Provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterisation of both the cells and the derived products Describes the preparation of stem cells and others for use in cell-based therapies – an area of burgeoning research Includes experimental examples to indicate expected results Covers regulatory issues from the UK, the EU and the USA and reviews how these are developing around the world Addresses the key issues of standardisation and validation with chapters on GLP and GMP for cell culture processes Delivering insight into the exciting world of biological medicines and directions for further investigation into specific topics, Medicines from Animal Cell Culture is an essential resource for researchers and technicians at all levels using cell culture within the pharmaceutical, biotechnology and biomedical industries. It is of value to laboratory managers in these industries and to all those interested in this topic alike.

Download or read book General Techniques of Cell Culture written by Maureen A. Harrison and published by Cambridge University Press. This book was released on 1997-10-13 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concise introduction to a major technique of cell biology laboratories for those new to the field.

Download or read book Animal Cell Culture written by Mohamed Al-Rubeai and published by Springer. This book was released on 2014-11-28 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: ​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Download or read book Animal Tissue Culture written by A Wilson Aruni and published by MJP Publisher. This book was released on 2019-06-10 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and tissues culture has been one of the first and foremost techniques paving for recent cutting-edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. It has played a substantial role in the developments of health care and prophylactics industries, thus serving the mankind. It has made the dream of producing cost-effective prophylactics, diagnostics and therapeutics come true and affordable. In the recent past, with the explosion of knowledge in the field of biotechnology, intensive research in being carried out, where undergraduate and post-graduate courses are being offered in this field. Even through more emphasis is being given to theory, a dearth of practical knowledge is lacking due to paucity of established tissue culture facilities.

Download or read book In Vitro Cultivation of Animal Cells written by Open Universiteit (Heerlen, Netherlands) and published by Butterworth-Heinemann. This book was released on 1993 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Both practical and theoretical issues of animal cell cultivation are described, including media formulation, the production and characterisation of cell issues from explants and the preservation of cell lines. The book investigates how pure cultures of animal cells may be isolated from their primary sources, examines the parameters which influence their growth in culture and explores how such parameters may be manipulated to modify cell yields.

Download or read book Animal Cell Bioreactors written by Chester S. Ho and published by Butterworth-Heinemann. This book was released on 2013-10-22 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. It addresses engineering aspects such as mass transfer, instrumentation, and control ensuring successful design and operation of animal cell bioreactors. The goal is to provide a comprehensive analysis and review in the advancement of the bioreactor systems for large-scale animal cell cultures. The book is organized into four parts. Part I traces the historical development of animal cell biotechnology. It presents examples of work in progress that seeks to make animal cell biotechnology processes as productive on a cost per unit of product basis as that achieved by other microbial systems. Part II includes chapters dealing with the implications of cell biology in animal cell biotechnology; protein-bound oligosaccharides and their structures; the development of serum-free media and its use in the production of biologically active substances; and the metabolism of mammalian cells. Part III focuses on animal cell cultivation, covering topics such as the fixed bed immobilized culture; three-dimensional microcarriers; and hydrodynamic phenomena in microcarrier cultures. Part IV discusses the design, operation, and control of animal cell bioreactors.

Download or read book Freshney s Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2021-02-17 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Download or read book Animal Cell Technology written by Leda Castilho and published by Taylor & Francis. This book was released on 2008-03-01 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.

Download or read book Animal Cell Biotechnology written by Nigel Jenkins and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

Download or read book Animal Cell Biotechnology written by Raymond Spier and published by . This book was released on 1985 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: