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Book Analysis of Progesterone Receptor Positive Cells in Relationship to Mammary Epithelial Cell Growth and Differentiation in Mouse Mammary Glands

Download or read book Analysis of Progesterone Receptor Positive Cells in Relationship to Mammary Epithelial Cell Growth and Differentiation in Mouse Mammary Glands written by Sharianne G. Louie and published by . This book was released on 2000 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book The Role of Mammary Epithelial stromal Cell Interactions in Relation to Growth Regulation by Estrogen and Progestin

Download or read book The Role of Mammary Epithelial stromal Cell Interactions in Relation to Growth Regulation by Estrogen and Progestin written by Shiqing Wang and published by . This book was released on 1994 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Intercellular Signalling in the Mammary Gland

Download or read book Intercellular Signalling in the Mammary Gland written by C.H. Knight and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: All being done, we went to Mrs Shipmans, who is a great butter-woman; and I did see there the most of milke and cream, and the cleanest, that I ever saw in my life (29 May 1661). Among others, Sir Wm. Petty did tell me that in good earnest, he hath in his will left such parts of his estate to him that could invent such and such things -as among others, that could discover truly the way of milk coming into the breasts of a woman ... (22 March 1665). My wife tells me that she hears that my poor aunt James hath had her breast cut off here in tow- her breast having long been out of order (5 May 1665). From the Diary of Samuel Pepys, published as The Shorter Pepys (edited by R. Latham), Penguin Books (1987) The long-standing ultimate importance of research on the mammary gland is illustrated by the importance attached to cows' milk for human consumption, to human lactation and to breast cancer by Samuel Pepys and his contemporaries in the middle of the 17th century. Research has tended to develop in isolation in these three areas of continuing contemporary importance largely because in most countries, the underlying science of agricultural productivity is funded separately from the underlying science of human health and welfare.

Book Is the Regenerative Capacity of the Mammary Gland Contained With Those Mammary Cells That Express the Progesterone Receptor Implications for Breast Cancer

Download or read book Is the Regenerative Capacity of the Mammary Gland Contained With Those Mammary Cells That Express the Progesterone Receptor Implications for Breast Cancer written by and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent transgenic studies have demonstrated that targeted overexpression of the A and B isoforms of the progesterone receptor (PR) to the mammary gland results in excessive ductal branching and alveolar growth respectively. Based on the above, we hypothesized that mammary-specific stem cells are present as a subgroup within those epithelial cells that express PR. To determine whether a stem cell population is contained within the PR expressing cell population (PR(+)), a PR-lacZ knockin mouse in combination with fluorescent-activated cell sorting (FACS) was utilized to separate PR(+) from PR( - ) mammary cells; both cell populations would then be evaluated for their regenerative capacity by transplantation into the cleared mammary fat pad of a host animal. FACS analysis applied to the PR-lacZ mammary gland provided a significantly enriched (70%) PR(+) mammary epithelial cell population that maintained lacZ expression in culture. Although, PR(+) and PR( - ) enriched mammary epithelial cell populations were transplanted into mammary fat pads, these host animals were lost to Tropical Storm 'Allison'; estimation of the regenerative potential of the above mammary epithelial cell populations will have to await the expansion of the PR-lacZ colony.

Book Proliferation of Estrogen Receptor Alpha Positive Mammary Epithelial Cells is Restrained by TGFbeta1 in Adult Mice

Download or read book Proliferation of Estrogen Receptor Alpha Positive Mammary Epithelial Cells is Restrained by TGFbeta1 in Adult Mice written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Transforming growth factor [beta]1 (TGF[beta]1) is a potent inhibitor of mammary epithelial proliferation. In human breast, estrogen receptor [alpha] (ER[alpha]) cells rarely co-localize with markers of proliferation, but their increased frequency correlates with breast cancer risk. To determine whether TGF[beta]1 is necessary for the quiescence of ER[alpha]-positive population, we examined mouse mammary epithelial gland at estrus. Approximately 35% of cells showed TGF[beta]1 activation, which co-localized with nuclear receptor-phosphorylated Smad 2/3, indicating that TGF[beta] signaling is autocrine. Furthermore, nuclear Smad co-localized with nuclear ER[alpha]. To test whether TGF[beta] was functional, we examined genetically engineered mice with different levels of TGF[beta]1. ER[alpha] co-localization with markers of proliferation (i.e. Ki-67 or BrdU) at estrus was significantly increased in the mammary glands of Tgf[beta]1 C57/bl/129SV heterozygote mice. This relationship was maintained following pregnancy, but was absent at puberty. Conversely, mammary epithelial expression of constitutively active TGF[beta]1 via the MMTV promoter suppressed proliferation of ER[alpha] positive cells. Thus, TGF[beta]1 activation functionally restrains ER[alpha] positive cells from proliferating in adult mammary gland. Accordingly, we propose that TGF[beta]1 dysregulation may promote proliferation of ER[alpha] positive cells associated with breast cancer risk in humans.

Book Mapping Putative Stem progenitor Cells in the Mouse and Rat Mammary Glands

Download or read book Mapping Putative Stem progenitor Cells in the Mouse and Rat Mammary Glands written by Diana A. Sallam and published by . This book was released on 2008 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Control of the Mammary Cell Cycle Clock by Estrogen and Progesterone

Download or read book Control of the Mammary Cell Cycle Clock by Estrogen and Progesterone written by and published by . This book was released on 2001 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt: Both the growth and the development of the mammary gland are controlled by the female hormones estrogen, prolactin and progesterone, and by interactions between the epithelial and stromal compartments of the breast. Changes in the regulation of any of these processes may lead to breast cancer. We have investigated the role of progesterone in the process of sidebranching and alveologenesis in the mammary gland using mice lacking the progesterone receptor which are defective in these processes. By reconstituting murine mammary glands in vivo, we have shown that the progesterone receptor is required only in epithelial cells for proper sidebranching and alveologenesis to occur. Our studies indicate that progesterone acts in a paracrine manner and suggest that Wnt-4 is a mediator of the paracrine signals released from progesterone receptor-positive cells. In addition, we have characterized the role of the estrogen receptor (ER) in regulating the proliferation of breast cancer cells. We postulate that the ability of ER to control cyclin Dl expression and proliferation of breast cancer cells may be acquired during breast cancer progression. This is indicated by the fact that ectopic expression of the estrogen receptor in human epithelial cells does not, on its own, enable signaling between the ER and the cyclin D1 gene. Moreover, others have demonstrated that the ER-positive cells in the mammary epithelium are distinguishable from those that are actively mitotic. Finally, our work indicates that the effects of deletion of the prolactin receptor (PrlR) from mammary epithelial cells closely phenocopy the consequences of deleting cyclin Dl from these cells. We suggest that activation of the PrlR by its ligand results in the production of insulin-like growth factor-2 which in turn induces cyclin D1 synthesis in these cells.

Book Progestins and the Mammary Gland

Download or read book Progestins and the Mammary Gland written by Orla M. Conneely and published by Springer Science & Business Media. This book was released on 2008-03-27 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progestins play a key role in reproductive endocrinology and as pharmaceutical drugs for contraception and in combined hormone therapy. To further our understanding of progestin action in the mammary gland, an international symposium, attended by leading researchers from academia and industry, was held in Berlin, 21–23 March 2007. Genetic mouse models helped to elucidate the role of progestins, both in normal breast development and in disease. Mechanistic molecular studies inspired the design of new progestins with improved tissue selectivity. In addition, the clinical impact of progesterone receptor agonists and antagonists for the prevention and treatment of breast cancer was discussed.

Book Hormonal and Nutritional Requirements for the Serum free Proliferation of Mouse Mammary Epithelial Cells in Vitro

Download or read book Hormonal and Nutritional Requirements for the Serum free Proliferation of Mouse Mammary Epithelial Cells in Vitro written by Walter Tsuyoshi Imagawa and published by . This book was released on 1983 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Microenvironment Growth Control of Mouse Mammary Epithelial Cells

Download or read book Microenvironment Growth Control of Mouse Mammary Epithelial Cells written by Hongmei Yu and published by . This book was released on 2006 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book The Role of Progesterone and Its Receptor in Mammary Gland Tumor Growth in Rats

Download or read book The Role of Progesterone and Its Receptor in Mammary Gland Tumor Growth in Rats written by Caroline Low and published by . This book was released on 2003 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book The Analysis of Cell Population Dynamics in Mammary Gland Development and Tumorigenesis

Download or read book The Analysis of Cell Population Dynamics in Mammary Gland Development and Tumorigenesis written by and published by . This book was released on 2006 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: The mammary gland is made up of two distinct epithelial layers that are derived from a common progenitor. The experiments in this proposal investigate the processes of mammary epithelial cell differentiation during development and tumorigenesis. Using FACS, mammary epithelial cell (MEC) populations from tumors and wildtype tissue were investigated for their outgrowth potential or tumorigenic capacity. We also developed new mouse models and imaging techniques that can be used to identify viable long-surviving cell populations and characterize these cells in real-time in vivo. We are also interested in studying how genes expressed in different mammary epithelial cell populations affect differentiation and tumorigenesis. For these experiments we have developed techniques for viral transduction and transplantation of primary MECs.