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Book Analysis of Escherichia Coli Populations in a Large Watershed

Download or read book Analysis of Escherichia Coli Populations in a Large Watershed written by Michelle D. Nemec and published by . This book was released on 2008 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: Escherichia coli are one of the most common model organisms used today. This organism has played an integral role in our understanding of bacterial conjugation, phage genetics, and gene structure, and it is also responsible for many of the advances in the areas of proteomics and biotechnology. However, very little is known about the characteristics of E. coli populations in their natural habitats. In this study, three characteristics of E. coli populations isolated from nine different sources were investigated, including: 1) antibiotic resistance, 2) carbon--substrate usage, and 3) genetic diversity. Antibiotic resistance was examined using eight antibiotics. Overall, the incidence of amoxicillin resistance was high in all of the E. coli populations, and multi-drug resistance was common. Antibiotic resistance was prevalent across populations, and the highest level of resistance was found in isolates obtained from sewage and dairy cattle. The carbon--substrate utilization of the E. coli populations was examined using Biolog GN2 microplates. The fundamental metabolic capabilities of the E. coli isolates were relatively stable across populations; however, variation did occur in the extent to which some isolates in the populations could utilize the various carbon substrates. Metabolic similarities were greatest between E. coli isolates from the same population. Rep--PCR was implemented to assess the genetic diversity present in the various E. coli populations. Overall, the genetic diversity of the E. coli isolates appeared to be large. Unfortunately, there are very few other studies available with which to compare these data. The level of genetic diversity varied between each population of isolates; although, isolates from the same population tended to be more genetically similar than isolates from different populations. Finally, Jackknife analysis demonstrated that the various E. coli populations were more distinct from each other genetically than they were based on antibiotic resistance or carbon--utilization. When all three characteristics were combined, relatively clear distinctions could be made between most populations. When the characteristics of E. coli isolated from water were compared with the characteristics of the isolates from the other sources, the majority of the water isolates were most similar to E. coli obtained from cattle.

Book Genetic Diversity of Escherichia Coli in Soils and Sediments of an Agricultural Watershed and Their Spatiotemporal Influences on Water Quality

Download or read book Genetic Diversity of Escherichia Coli in Soils and Sediments of an Agricultural Watershed and Their Spatiotemporal Influences on Water Quality written by Gregory Stuart Piorkowski and published by . This book was released on 2013 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT Cont.'d: The relationship of land use type to particle attachment reinforces the hypothesis that strain-specificity in attachment behaviour can affect the transport of E. coli in fluvial systems. This work provides evidence that putatively naturalized strains in cultivated fields can contribute a large part to waterborne E. coli, and that reach-specific hydrological factors need to be considered when relating sediment- to waterborne E. coli in fluvial systems.

Book Development and Application of the Spatially Explicit Load Enrichment Calculation Tool  select  to Determine Potential E  Coli Loads in Watersheds

Download or read book Development and Application of the Spatially Explicit Load Enrichment Calculation Tool select to Determine Potential E Coli Loads in Watersheds written by Kendra Jean Riebschleager and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: According to the USEPA National Section 303(d) List Fact Sheet, bacterial pathogens are the leading cause of water quality impairments in Texas. The automated Spatially Explicit Load Enrichment Calculation Tool (SELECT) uses spatially variable factors such as land use, soil condition, and distance to streams to characterize pathogen sources across a watershed. The results support development of Total Maximum Daily Loads (TMDLs) where bacterial contamination is of concern. SELECT calculates potential E. coli loads by distributing the contributing source populations across suitable habitats, applying a fecal production rate, and then aggregating the potential load to the subwatersheds. SELECT provides a Graphical User Interface (GUI), developed in Visual Basic for Applications (VBA) within ArcGIS 9.X, where project parameters can be adjusted for various pollutant loading scenarios. A new approach for characterizing E. coli loads resulting from on-site wastewater treatment systems (OWTSs) was incorporated into the SELECT methodology. The pollutant connectivity factor (PCF) module was created to identify areas potentially contributing E. coli loads to waterbodies during runoff events by weighting the influence of potential loading, runoff potential, and travel distance. Simulation results indicate livestock and wildlife are potentially contributing large amounts of E. coli in the Lake Granbury Watershed in areas where these contributing sources are not currently monitored for E. coli. The bacterial water quality violations near Lake Granbury are most likely the result of malfunctioning OWTSs and pet waste in the runoff. The automated SELECT was verified by characterizing the potential E. coli loading in the Plum Creek Watershed and comparing to results from a prior study (Teague, 2007). The E. coli potential load for the watershed was lower than the previous study due to major differences in assumptions. Comparing the average ranked PCF estimated by physical properties of the watershed with the statistical clustering of watershed characteristics provided similar groupings. SELECT supports the need to evaluate each contributing source separately to effectively allocate site specific best management practices (BMPs). This approach can be used as a screening step for determining areas where detailed investigation is merited. SELECT in conjunction with PCF and clustering analysis can assist decision makers develop Watershed Protection Plans (WPPs) and determine TMDLs.

Book Microbiology of Waterborne Diseases

Download or read book Microbiology of Waterborne Diseases written by and published by Academic Press. This book was released on 2013-11-08 with total page 719 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Microbiology of Waterborne Diseases describes the diseases associated with water, their causative agents and the ways in which they gain access to water systems. The book is divided into sections covering bacteria, protozoa, and viruses. Other sections detail methods for detecting and identifying waterborne microorganisms, and the ways in which they are removed from water, including chlorine, ozone, and ultraviolet disinfection. The second edition of this handbook has been updated with information on biofilms and antimicrobial resistance. The impact of global warming and climate change phenomena on waterborne illnesses are also discussed. This book serves as an indispensable reference for public health microbiologists, water utility scientists, research water pollution microbiologists environmental health officers, consultants in communicable disease control and microbial water pollution students. Focuses on the microorganisms of most significance to public health, including E. coli, cryptosporidium, and enterovirus Highlights the basic microbiology, clinical features, survival in the environment, and gives a risk assessment for each pathogen Contains new material on antimicrobial resistance and biofilms Covers drinking water and both marine and freshwater recreational bathing waters

Book Determining the Potential Sources of Escherichia Coli in the South Bosque Watershed Using Antibiotic Resistance Analysis and Carbon Utilization Profiling

Download or read book Determining the Potential Sources of Escherichia Coli in the South Bosque Watershed Using Antibiotic Resistance Analysis and Carbon Utilization Profiling written by Samir H. Moussa and published by . This book was released on 2005 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Microbial Source Tracking

    Book Details:
  • Author : Jorge W. Santo Domingo
  • Publisher : Emerging Issues in Food Safety
  • Release : 2007
  • ISBN : 9781555813741
  • Pages : 285 pages

Download or read book Microbial Source Tracking written by Jorge W. Santo Domingo and published by Emerging Issues in Food Safety. This book was released on 2007 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.

Book Dissertation Abstracts International

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2009 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Stream Hydrology

Download or read book Stream Hydrology written by Nancy D. Gordon and published by John Wiley & Sons. This book was released on 2013-05-03 with total page 637 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition (1994) there have been rapid developments in the application of hydrology, geomorphology and ecology to stream management. In particular, growth has occurred in the areas of stream rehabilitation and the evaluation of environmental flow needs. The concept of stream health has been adopted as a way of assessing stream resources and setting management goals. Stream Hydrology: An Introduction for Ecologists Second Edition documents recent research and practice in these areas. Chapters provide information on sampling, field techniques, stream analysis, the hydrodynamics of moving water, channel form, sediment transport and commonly used statistical methods such as flow duration and flood frequency analysis. Methods are presented from engineering hydrology, fluvial geomorphology and hydraulics with examples of their biological implications. This book demonstrates how these fields are linked and utilised in modern, scientific river management. * Emphasis on applications, from collecting and analysing field measurements to using data and tools in stream management. * Updated to include new sections on environmental flows, rehabilitation, measuring stream health and stream classification. * Critical reviews of the successes and failures of implementation. * Revised and updated windows-based AQUAPAK software. This book is essential reading for 2nd/3rd year undergraduates and postgraduates of hydrology, stream ecology and fisheries science in Departments of Physical Geography, Biology, Environmental Science, Landscape Ecology, Environmental Engineering and Limnology. It would be valuable reading for professionals working in stream ecology, fisheries science and habitat management, environmental consultants and engineers.

Book Numerical Ecology with R

Download or read book Numerical Ecology with R written by Daniel Borcard and published by Springer. This book was released on 2018-03-19 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of Numerical Ecology with R guides readers through an applied exploration of the major methods of multivariate data analysis, as seen through the eyes of three ecologists. It provides a bridge between a textbook of numerical ecology and the implementation of this discipline in the R language. The book begins by examining some exploratory approaches. It proceeds logically with the construction of the key building blocks of most methods, i.e. association measures and matrices, and then submits example data to three families of approaches: clustering, ordination and canonical ordination. The last two chapters make use of these methods to explore important and contemporary issues in ecology: the analysis of spatial structures and of community diversity. The aims of methods thus range from descriptive to explanatory and predictive and encompass a wide variety of approaches that should provide readers with an extensive toolbox that can address a wide palette of questions arising in contemporary multivariate ecological analysis. The second edition of this book features a complete revision to the R code and offers improved procedures and more diverse applications of the major methods. It also highlights important changes in the methods and expands upon topics such as multiple correspondence analysis, principal response curves and co-correspondence analysis. New features include the study of relationships between species traits and the environment, and community diversity analysis. This book is aimed at professional researchers, practitioners, graduate students and teachers in ecology, environmental science and engineering, and in related fields such as oceanography, molecular ecology, agriculture and soil science, who already have a background in general and multivariate statistics and wish to apply this knowledge to their data using the R language, as well as people willing to accompany their disciplinary learning with practical applications. People from other fields (e.g. geology, geography, paleoecology, phylogenetics, anthropology, the social and education sciences, etc.) may also benefit from the materials presented in this book. Users are invited to use this book as a teaching companion at the computer. All the necessary data files, the scripts used in the chapters, as well as extra R functions and packages written by the authors of the book, are available online (URL: http://adn.biol.umontreal.ca/~numericalecology/numecolR/).

Book Water  a Reflection of Land Use

Download or read book Water a Reflection of Land Use written by Malin Falkenmark and published by . This book was released on 1999 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Integrated Water Resources Research

Download or read book Integrated Water Resources Research written by Jason A. Hubbart and published by MDPI. This book was released on 2021-05-20 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Anthropogenic and natural disturbances to freshwater quantity and quality are a greater issue for society than ever before. To successfully restore water resources requires understanding the interactions between hydrology, climate, land use, water quality, ecology, and social and economic pressures. This Special Issue of Water includes cutting edge research broadly addressing investigative areas related to experimental study designs and modeling, freshwater pollutants of concern, and human dimensions of water use and management. Results demonstrate the immense, globally transferable value of the experimental watershed approach, the relevance and critical importance of current integrated studies of pollutants of concern, and the imperative to include human sociological and economic processes in water resources investigations. In spite of the latest progress, as demonstrated in this Special Issue, managers remain insufficiently informed to make the best water resource decisions amidst combined influences of land use change, rapid ongoing human population growth, and changing environmental conditions. There is, thus, a persistent need for further advancements in integrated and interdisciplinary research to improve the scientific understanding, management, and future sustainability of water resources.

Book Scientific Investigations Report

Download or read book Scientific Investigations Report written by Sharon E. Kroening and published by . This book was released on 2004 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Multivariate Analysis of Ecological Data Using CANOCO

Download or read book Multivariate Analysis of Ecological Data Using CANOCO written by Jan Lepš and published by Cambridge University Press. This book was released on 2003-05-29 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Table of contents

Book Population Genetics of Bacteria

Download or read book Population Genetics of Bacteria written by Seth T. Walk and published by American Society for Microbiology Press. This book was released on 2011-04-20 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revisit the work of a pioneering innovator… • Explores the field of bacterial population genetics by highlighting the work of Thomas S. Whittam, best known for his work with enterohemorrhagic E. coli. • Features a compilation of research projects and ideas stemming from Dr. Whittam’s work that presents a broad perspective on the historical development of bacterial population genetics.

Book Protocol for Developing Pathogen TMDLs

Download or read book Protocol for Developing Pathogen TMDLs written by and published by . This book was released on 2001 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: