Download or read book An Atlas of the Ultrastructure of Human Oocytes written by M. El Shafie and published by CRC Press. This book was released on 2000-06-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This ground-breaking atlas of oocyte ultrastructure forms a practical guide to assisted reproduction. Extensively illustrated with transmission electron microscopic (TEM) images, it contains chapters on oocyte maturation, fertilization, fertilization failure, pitfalls and technical problems of intracytoplasmic sperm injection, and failed fertilization in vitro to explain the principles and evaluation of TEM images. A large chapter of TEM images of the ultrastructure of human oocytes illustrates fertilization failures after ICSI and IVF and the ultrastructure of germinal vesicle oocytes and metaphase I oocytes. It also contains detailed information about abnormal metaphase II oocytes with sections on autolysis, multi-vacuolated mature oocyte, refractile body, and fragmented oocyte. The concluding chapter describes materials and methods for single-cell transmission electron microscopy of the human oocyte.
Download or read book An Atlas of the Ultrastructure of Human Oocytes written by M. El Shafie and published by CRC Press. This book was released on 2000-06-15 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This ground-breaking atlas of oocyte ultrastructure forms a practical guide to assisted reproduction. Extensively illustrated with transmission electron microscopic (TEM) images, it contains chapters on oocyte maturation, fertilization, fertilization failure, pitfalls and technical problems of intracytoplasmic sperm injection, and failed fertilization in vitro to explain the principles and evaluation of TEM images. A large chapter of TEM images of the ultrastructure of human oocytes illustrates fertilization failures after ICSI and IVF and the ultrastructure of germinal vesicle oocytes and metaphase I oocytes. It also contains detailed information about abnormal metaphase II oocytes with sections on autolysis, multi-vacuolated mature oocyte, refractile body, and fragmented oocyte. The concluding chapter describes materials and methods for single-cell transmission electron microscopy of the human oocyte.
Download or read book HUMAN CELL AND TISSUE FINE STRUCTURE FOR TEACHING AND RESEARCH IN STEM CELLS written by PROFESSOR ARUNACHALAM HENRY SATHANANTHAN and published by Professor Arunachalam Henry Sathananthan . This book was released on 2015-01-06 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This EBook covers the fine structure of human cells and tissues as seen with the transmission and scanning electron microscope (TEM & SEM). To the author’s knowledge there is no book of this kind expressly devoted to human cells and tissues. The book is concise and is primarily intended to help in the teaching of microanatomy to first-year medical and health-science students, paramedical students and first-year science and other university students. It can also be used to teach university entrance students in secondary schools and technical staff in anatomical pathology in hospitals and specifically those involved in stem cell research. There are innumerable texts in light microscopy (LM) of basic histology that are now available for comparison to all and on line, particularly on Google, Wikipedia, PubMed and other search engines. Microanatomy is essentially a visual subject and the author firmly believes that a picture is worth a thousand words. The cell is the fundamental unit of structure in the human body. Cells and their products form the tissues and the various organs and organ systems of the human body. Understanding their structure is not only basic to microanatomy it is also of importance in the study of physiology and pathology and of course, gross anatomy. Now with dawn of stem cell research, it can be used as guide to understand adult and embryonic stem cell microstructure in conjunction with LM and immuno -fluorescent microscopy (FM). As an innovation to the original atlas we have added, exquisite colour images (SEM) by Prof. Pietro Motta, a world leader in electron microscopy, author and publisher of many atlases aided by his co-workers in La Sapienza, University of Roma, Italy, to appreciate the third dimension in microstructure. Some images of the testis are credited to Professors. David de Kretser & Jeff. Kerr, my colleagues at Monash University. Prof. de Kretser, of course, is one of my role models since he is an electron microscopist, clinician and expert on the testis and male infertility. He was founder Director of the Institute of Reproduction & Development, where I was honorary associate professor. He is also a born Sri Lankan and was Governor of Victoria. To help interpretation of the electron micrographs, the structure of each type of cell and/or tissue is illustrated diagramatically, and an attempt has been made to relate this to function. Where possible, such interpretative diagrams are printed adjacent to the electron micrographs of that particular type of cell/ tissue. Some of these diagrams were coloured by computer. In addition, brief descriptions of the anatomy of the cells/tissues and legends that describe the electron micrograph are included. Each section will briefly introduce the reader to the type of cell, tissue or organ that is being illustrated. Since there are many advanced atlases and textbooks on the fine structure of cells and tissues, the present publication is intended to be a simple reference for the student and researcher. One of the greatest difficulties readers have in the interpretation of cell structure using LM is that they do not see the outlines of cells and for the most part they do not see the internal structure of the cell very clearly. This is because the cell membrane and most of the internal structures are beyond the high resolution of the LM. Electron microscopy, on the other hand, magnifies cell organelles and enhances their resolution, making the interpretation of cell structure more precise and objective. However, there are limitations in the study of ultrastructure since only a very small section of the cell is viewed. Electron microscopy, as we all know, is laborious and very time consuming and has been used widely in biomedical research since 1935. We were the first to study embryonic stem cells by TEM, a logical progression of our extensive research on human gametes, fertilization and embryos in IVF & ART. The reader is advised to study images of cells and tissues in semi- thin epoxy sections (LM). This EBook (atlas) will be a valuable supplement to the numerous textbooks of histology, especially those with colour LMs of wax and epoxy sections. It covers the ultrastructure of the human cell, the basic tissues of the human body and some of the more important organs of the human body. It is specifically targeted to researchers involved in current stem cell research (both adult and embryonic). Finally, this publication is not intended to be a complete atlas of human cells and tissues since there are several excellent publications for the advanced study of electron microscopy, a few listed in the references.
Download or read book Atlas of Fine Structure of Human Sperm Penetration Eggs and Embryos Cultured in Vitro written by A. Henry Sathananthan and published by Greenwood. This book was released on 1986 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Atlas of the Ultrastructure of Human Oocytes written by M. El Shafie and published by CRC Press. This book was released on 2000-06-15 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This ground-breaking atlas of oocyte ultrastructure forms a practical guide to assisted reproduction. Extensively illustrated with transmission electron microscopic (TEM) images, it contains chapters on oocyte maturation, fertilization, fertilization failure, pitfalls and technical problems of intracytoplasmic sperm injection, and failed fertilization in vitro to explain the principles and evaluation of TEM images. A large chapter of TEM images of the ultrastructure of human oocytes illustrates fertilization failures after ICSI and IVF and the ultrastructure of germinal vesicle oocytes and metaphase I oocytes. It also contains detailed information about abnormal metaphase II oocytes with sections on autolysis, multi-vacuolated mature oocyte, refractile body, and fragmented oocyte. The concluding chapter describes materials and methods for single-cell transmission electron microscopy of the human oocyte.
Download or read book A Color Atlas for Human Assisted Reproduction written by Pasquale Patrizio and published by Lippincott Williams & Wilkins. This book was released on 2003 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Featuring more than 300 full-color photographs, this atlas is a comprehensive guide to the complex procedures used in assisted reproduction. It shows readers how to utilize the latest technologies and correlates the laboratory and clinical components of assisted reproduction. The book depicts oocytes, embryos, and blastocysts at various stages of division and offers guidelines for assessing oocytes and embryos. The authors give detailed instructions on ovarian stimulation, intracytoplasmic sperm injection, assisted hatching, cryopreservation, extended in vitro culturing, preimplantation genetic diagnosis, and embryo transfer techniques. Close attention is also given to assessment of oocytes and embryos from patients with endometriosis and other pathologies.
Download or read book Atlas of the Human Oocyte and Early Conceptus written by Lucinda L. Veeck and published by . This book was released on 1991 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Atlas of Human Reproduction written by E.S. Hafez and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: The suggestion of Max Knoll that an electron fascinated by the numerous SEM photographs, the wealth of information and the enthusiasm of the microscope could be developed using a fine scanning researchers covering a variety of disciplines. All aspects beam of electrons on a specimen surface and recording the emitted current as a function of the position of the of the female and male genital tract have been covered, beam was launched in 1935. Since then several culminating in the prizewinning award showing the in investigators and clinicians have used this concept to vitro fertilized human egg. develop techniques now known as scanning electron In clinical diagnostics SEM also proved to be a microscopy (SEM) and scanning transmission electron valuable complementary technique, shedding new light microscopy (STEM). The choice to study the female on oncology, the pathogenesis of tubal disease and the reproductive organs was a logical one because cells and maturation process of the placenta. Future research has tissue samples can be sampled relatively easily; still to be accomplished; e.g. quantification of SEM furthermore, these cells and organs are influenced photographs for meaningful and sound biological, continuously by the cyclic production of hormones. scientific and statistical evaluation in diagnostic This atlas demonstrates the state of the art in 1983. gynecology, obstetrics, andrology and oncology.
Download or read book Practical Manual of In Vitro Fertilization written by Zsolt Peter Nagy and published by Springer Science & Business Media. This book was released on 2012-04-23 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.
Download or read book Biology and Pathology of the Oocyte written by Alan O. Trounson and published by Cambridge University Press. This book was released on 2003-07-10 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human oocyte or egg is the rarest and most rapidly ageing cell in the body, and yet one that is essential for fertility. This book is about the development, biology and pathology of the oocyte, and new technologies to manipulate, enhance, and control fertility. These technologies are paving the way for overcoming infertility, avoiding inherited diseases, and creating genetically engineered animals from embryo stem cells and cloning. This progress would have been impossible without the myriad of scientific and technical developments covered in this book, including the ability to manipulate and fertilize oocytes in vitro. World-leading experts have contributed to the first book for many years on basic and applied science of the egg, its clinical manipulation, and its pivotal role in reproductive medicine and biology. It will be an essential reference for scientists and clinicians in reproductive medicine and animal breeding technology, and everyone involved in treating infertility.
Download or read book Microscopic Images of Human Sperm Oocytes and Embryos for Assisted Reproductive Technology Including Embryonic Stem Cells written by Professor Arunachalam Henry Sathananthan and published by Professor Arunachalam Henry Sathananthan . This book was released on 2014-12-29 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Epub incorporates 37 years of embodied research at Monash & La Trobe Universities, Melbourne, by the author with his colleagues in Australia and overseas (England, Singapore, India, Sri Lanka, Italy & Japan).It is a multi-author, visual presentation of the microscopic structure of human oocytes, sperm and embryos in conjunction with clinical IVF and ART and includes embryonic stem cells, a logical extension of ART.Most of the images were taken with electron microscopes (TEM & SEM), light and phase microscopes (LM) and fluorescent microscopes (FM) with diagrams.Both published micrographs (from our atlases and journals) and also original unpublished micrographs are presented. The printed atlases were scanned and new material was provided on PPT, PDF & YouTube for EPub.The aim of this EPub is to make our research available online for clinicians, scientists & students and posterity. A. Henry Sathananthan (Adjunct Professor, Anatomy & Developmental Biology,Medical Faculty, Monash University, Australia)
Download or read book Manual of Oocyte Retrieval and Preparation in Human Assisted Reproduction written by Rachel Cutting and published by Cambridge University Press. This book was released on 2022-10-31 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive manual aids navigation of the complex process of oocyte retrieval and preparation, with tips for optimizing outcomes.
Download or read book Ultrastructure of Human Gametogenesis and Early Embryogenesis written by Jonathan van Blerkom and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few years, the adoption and worldwide proliferation of clinical procedures for medically assisted conception have been associated with the examination and analysis of spermatozoa, oocytes and early embryos under a variety of in vivo and in vitro conditions. These analyses have enabled correlations to be made between the behavior of gametes, the pattern of early embryonic development and the initiation of a normal pregnancy. Collectively, the findings have not only enormously increased our understanding of the process of early human development, but also have provided new insights into the origin and causes of reproductive failure in man. The research presented in this volume describes recent results derived from the study of normal and abnormal patterns of human spermatogenesis, oogenesis and early embryogenesis. The chapters discuss aberrations in morphodynamic and morphophysiological processes that have clinical relevance in human infertility and conception. Two of the chapters describe, respectively, the basic research that allows the cryopreservation of human oocytes and embryos, and the development of in vitro systems that permit the study of cell differentiation and interaction during the peri-implantation period. When relevant, each chapter extrapolates findings from in vitro experimentation to the comparable situation that is observed in vivo.
Download or read book Atlas of Human Histology and Ultrastructure written by James Les Matthews and published by . This book was released on 1971 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Culture Media Solutions and Systems in Human ART written by Patrick Quinn and published by Cambridge University Press. This book was released on 2014-03-27 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.
Download or read book Ultrastructure of the Ovary written by G. Familiari and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Preimplantation Embryo Selection written by Jacques Cohen and published by CRC Press. This book was released on 2007-09-26 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most profound dilemma in assisted reproduction to date is the inability to recognize potentially viable embryos before their replacement into the reproductive tract. Application of increasingly advanced new technology has allowed the field of embryo evaluation to evolve rapidly and dramatically over the past five years.Human Preimplantation Emb
