Download or read book Alternative Substrates for Estimating TCE degrading Capabilities of Toluene oxidizing Bacteria written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the primary impediments to the implementation of bioremediation is uncertainty about success in the field. Soils and microbial populations are heterogeneous and it is difficult to extrapolate biodegradation rates from small samples to field scale. While biodegradation rates can be estimated from microcosm studies, in situ methods offer a more meaningful gauge of resident microbial activity. One method used to estimate biodegradation rates in the field is the newly developed Push-Pull technique. While this technique can be conducted on site, it is normally not possible to use target pollutants as the reactive substrates. Consequently, alternative, benign reactive tracers must be used. Ideally, these alternative, reactive tracers interrogate the same enzyme systems that are responsible for the biodegradation of the target pollutant. The objective of this study was to develop a reactive tracer system that could be used to assess toluene-dependent trichloroethylene (TCE) degradation. Our approach has been to determine whether a series of pure strains of toluene-oxidizing bacteria (Burkholderia cepacia G4, Pseudomonas putida F1, Pseudomonas putida mt2, Pseudomonas mendocina KR1), each with different toluene-oxidizing enzymes systems, are capable of cometabolically oxidizing a series of eleven potential alternative substrates. These substrates include simple alkenes, alkanes, and cyclic alkanes. The kinetics (Ks and Vmax) of the biotransformation of these compounds have been determined. While oxidation products were observed for a number of these substrates in connection with one or more of the test organisms, isobutylene was co-oxidized by all test organisms. Oxidation of isobutylene by each organism yielded kinetics constants comparable to the corresponding kinetics of TCE degradation. The enzyme system expressed by Burkholderia cepacia G4 catalyzed the epoxidation of isobutylene while the remaining enzyme systems catalyzed allylic alcohol formation. Isobutylene.
Download or read book Alternative Substrates for Estimating TCE degrading Capabilities of Toluene oxidizing Bacteria written by Kristin Hicks and published by . This book was released on 2002 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: Michaelis-Menten, toluene dioxygenase, toluene monooxygenase.
Download or read book Consequences of Microbial Interactions with Hydrocarbons Oils and Lipids Biodegradation and Bioremediation written by Robert J. Steffan and published by Springer. This book was released on 2019-08-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book international experts discuss the state-of-the-art in the biological degradation of hydrocarbons to meet remedial or disposal goals. The work focuses on practical applications, often on globally important scales including the remediation of some of the world’s largest crude oil spills. Other related chapters discuss important implications of microbial transformation of hydrocarbons, including treatment of high fat processing wastes, impacts of microbial biodegradation activity on industrial processes, and the implications of microbial oil degradation in relation to modern oil extraction processes like hydraulic fracturing of shales and extraction of oil sands.
Download or read book Applied and Environmental Microbiology written by and published by . This book was released on 1998 with total page 856 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Situ Bioremediation written by National Research Council and published by National Academies Press. This book was released on 1993-02-01 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ bioremediationâ€"the use of microorganisms for on-site removal of contaminantsâ€"is potentially cheaper, faster, and safer than conventional cleanup methods. But in situ bioremediation is also clouded in uncertainty, controversy, and mistrust. This volume from the National Research Council provides direction for decisionmakers and offers detailed and readable explanations of: the processes involved in in situ bioremediation, circumstances in which it is best used, and methods of measurement, field testing, and modeling to evaluate the results of bioremediation projects. Bioremediation experts representing academic research, field practice, regulation, and industry provide accessible information and case examples; they explore how in situ bioremediation works, how it has developed since its first commercial use in 1972, and what research and education efforts are recommended for the future. The volume includes a series of perspective papers. The book will be immediately useful to policymakers, regulators, bioremediation practitioners and purchasers, environmental groups, concerned citizens, faculty, and students.
Download or read book Natural Attenuation for Groundwater Remediation written by Commission on Geosciences, Environment, and Resources and published by National Academies Press. This book was released on 2000-08-31 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, officials responsible for clean-up of contaminated groundwater have increasingly turned to natural attenuation-essentially allowing naturally occurring processes to reduce the toxic potential of contaminants-versus engineered solutions. This saves both money and headaches. To the people in surrounding communities, though, it can appear that clean-up officials are simply walking away from contaminated sites. When is natural attenuation the appropriate approach to a clean-up? This book presents the consensus of a diverse committee, informed by the views of researchers, regulators, and community activists. The committee reviews the likely effectiveness of natural attenuation with different classes of contaminants-and describes how to evaluate the "footprints" of natural attenuation at a site to determine whether natural processes will provide adequate clean-up. Included are recommendations for regulatory change. The committee emphasizes the importance of the public's belief and attitudes toward remediation and provides guidance on involving community stakeholders throughout the clean-up process. The book explores how contamination occurs, explaining concepts and terms, and includes case studies from the Hanford nuclear site, military bases, as well as other sites. It provides historical background and important data on clean-up processes and goes on to offer critical reviews of 14 published protocols for evaluating natural attenuation.
Download or read book Cellular Ecophysiology of Microbe written by Tino Krell and published by Springer. This book was released on 2018-03-24 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book assembles concisely written chapters by world-leaders in the field summarizing recent advances in understanding microbial responses to hydrocarbons. Subjects treated include mechanisms of sensing, hydrocarbon tolerance and degradation as well as an overview on hydrophobic modification of biomolecules. Other chapters are dedicated to issues related to the reduced bioavailability of hydrocarbons, which differentiates this class of compounds form many others, but which of central importance to understand the ecophysiological consequences. This book should be standard literature in any laboratory working in this area.
Download or read book In Situ Bioreclamation written by Robert E. Hinchee and published by Elsevier. This book was released on 2013-10-22 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Situ Bioreclamation: Applications and Investigations for Hydrocarbon and Contaminated Site Remediation is a collection of selected papers submitted by participants to the international symposium ""In Situ and On-Site Bioreclamation"", held in San Diego, California in March 1991. The book consists of articles, which represent a substantial technical contribution, and technical notes, and brief technology descriptions or reports of preliminary or less substantial studies that proposes and exposes various solutions for the biological treatment of contaminated soil, water, and gas. This volume is one of two that represent the most complete and up-to-date set of papers at the time. The book covers the most comprehensive collection of treatises that presents practices in the reversal of damages to the environment. Solutions vary from proven commercially available technologies for some applications, such as reactor treatment of petroleum hydrocarbons in aqueous steams, to fundamental research in others, such as genetic engineering to develop strains of biodegradable xenobiotics. Environmentalists, scientists, students of natural sciences, civil engineers, and those concerned with the preservation and restoration of nature will find this book invaluable.
Download or read book Biodegradation and Bioremediation written by Ajay Singh and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this volume, experts from universities, government labs and industry share their findings on the microbiological, biochemical and molecular aspects of biodegradation and bioremediation. The text covers numerous topics, including: bioavailability, biodegradation of various pollutants, microbial community dynamics, properties and engineering of important biocatalysts, and methods for monitoring bioremediation processes. Microbial processes are environmentally compatible and can be integrated with non-biological processes to detoxify, degrade and immobilize environmental contaminants.
Download or read book Alternatives for Ground Water Cleanup written by National Research Council and published by National Academies Press. This book was released on 1994-02-01 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: There may be nearly 300,000 waste sites in the United States where ground water and soil are contaminated. Yet recent studies question whether existing technologies can restore contaminated ground water to drinking water standards, which is the goal for most sites and the result expected by the public. How can the nation balance public health, technological realities, and cost when addressing ground water cleanup? This new volume offers specific conclusions, outlines research needs, and recommends policies that are technologically sound while still protecting health and the environment. Authored by the top experts from industry and academia, this volume: Examines how the physical, chemical, and biological characteristics of the subsurface environment, as well as the properties of contaminants, complicate the cleanup task. Reviews the limitations of widely used conventional pump-and-treat cleanup systems, including detailed case studies. Evaluates a range of innovative cleanup technologies and the barriers to their full implementation. Presents specific recommendations for policies and practices in evaluating contamination sites, in choosing remediation technologies, and in setting appropriate cleanup goals.
Download or read book Alternatives for Managing the Nation s Complex Contaminated Groundwater Sites written by National Research Council and published by National Academies Press. This book was released on 2013-02-27 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Across the United States, thousands of hazardous waste sites are contaminated with chemicals that prevent the underlying groundwater from meeting drinking water standards. These include Superfund sites and other facilities that handle and dispose of hazardous waste, active and inactive dry cleaners, and leaking underground storage tanks; many are at federal facilities such as military installations. While many sites have been closed over the past 30 years through cleanup programs run by the U.S. Department of Defense, the U.S. EPA, and other state and federal agencies, the remaining caseload is much more difficult to address because the nature of the contamination and subsurface conditions make it difficult to achieve drinking water standards in the affected groundwater. Alternatives for Managing the Nation's Complex Contaminated Groundwater Sites estimates that at least 126,000 sites across the U.S. still have contaminated groundwater, and their closure is expected to cost at least $110 billion to $127 billion. About 10 percent of these sites are considered "complex," meaning restoration is unlikely to be achieved in the next 50 to 100 years due to technological limitations. At sites where contaminant concentrations have plateaued at levels above cleanup goals despite active efforts, the report recommends evaluating whether the sites should transition to long-term management, where risks would be monitored and harmful exposures prevented, but at reduced costs.
Download or read book Groundwater and Soil Cleanup written by National Research Council and published by National Academies Press. This book was released on 1999-11-21 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive, up-to-date review of technologies for cleaning up contaminants in groundwater and soil. It provides a special focus on three classes of contaminants that have proven very difficult to treat once released to the subsurface: metals, radionuclides, and dense nonaqueous-phase liquids such as chlorinated solvents. Groundwater and Soil Cleanup was commissioned by the Department of Energy (DOE) as part of its program to clean up contamination in the nuclear weapons production complex. In addition to a review of remediation technologies, the book describes new trends in regulation of contaminated sites and assesses DOE's program for developing new subsurface cleanup technologies.
Download or read book In Situ Bioremediation of Ground Water and Geological Material written by Robert D. Norris and published by DIANE Publishing. This book was released on 1995-08 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a detailed background of the technologies available for the bioremediation of contaminated soil & ground water. Prepared for scientists, consultants, regulatory personnel, & others who are associated in some way with the restoration of soil & ground water at hazardous waste sites. Also provides insights to emerging technologies which are at the research level of formation, ranging from theoretical concepts, through bench scale inquiries, to limited field-scale investigations. 95 tables & figures.
Download or read book Encyclopedia of Ecology written by Brian D. Fath and published by Newnes. This book was released on 2014-11-03 with total page 4292 pages. Available in PDF, EPUB and Kindle. Book excerpt: The groundbreaking Encyclopedia of Ecology provides an authoritative and comprehensive coverage of the complete field of ecology, from general to applied. It includes over 500 detailed entries, structured to provide the user with complete coverage of the core knowledge, accessed as intuitively as possible, and heavily cross-referenced. Written by an international team of leading experts, this revolutionary encyclopedia will serve as a one-stop-shop to concise, stand-alone articles to be used as a point of entry for undergraduate students, or as a tool for active researchers looking for the latest information in the field. Entries cover a range of topics, including: Behavioral Ecology Ecological Processes Ecological Modeling Ecological Engineering Ecological Indicators Ecological Informatics Ecosystems Ecotoxicology Evolutionary Ecology General Ecology Global Ecology Human Ecology System Ecology The first reference work to cover all aspects of ecology, from basic to applied Over 500 concise, stand-alone articles are written by prominent leaders in the field Article text is supported by full-color photos, drawings, tables, and other visual material Fully indexed and cross referenced with detailed references for further study Writing level is suited to both the expert and non-expert Available electronically on ScienceDirect shortly upon publication
Download or read book Contaminants in the Subsurface written by National Research Council and published by National Academies Press. This book was released on 2005-04-23 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: At hundreds of thousands of commercial, industrial, and military sites across the country, subsurface materials including groundwater are contaminated with chemical waste. The last decade has seen growing interest in using aggressive source remediation technologies to remove contaminants from the subsurface, but there is limited understanding of (1) the effectiveness of these technologies and (2) the overall effect of mass removal on groundwater quality. This report reviews the suite of technologies available for source remediation and their ability to reach a variety of cleanup goals, from meeting regulatory standards for groundwater to reducing costs. The report proposes elements of a protocol for accomplishing source remediation that should enable project managers to decide whether and how to pursue source remediation at their sites.
Download or read book Novel Approaches for Bioremediation of Organic Pollution written by Raffi Fass and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the 42nd OHOLO Conference held in Eilat, Israel, May 3-7, 1998
Download or read book Enzymatic Plastic Degradation written by and published by Academic Press. This book was released on 2021-02-10 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Covers the latest research and technologies in enzymatic plastic degradation
