Download or read book Aeromonas Salmonicida and Aeromonas Hydrophila liquefaciens as Pathogens of Salmonid Fish A Selective Aeromonas Medium B Comparative Characteristics of Virulent and Avirulent Strains of Aeromonas Salmonicida C Effect of Water Temperature on Aeromonas Infections written by Ralph Hines McCoy and published by . This book was released on 1973 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies were directed toward the formulation of a selective Aeromonas medium that would permit the isolation of Aeromonas species in the presence of other common bacterial flora. The final composition of this medium, PBG Agar, in grams per liter, is: Bacto-peptone, 10; Bacto-beef extract, 10; glycogen, 4; NaCl, 5; sodium lauryl sulfate, 0. 1; brom thymol blue, 0. 1; agar, 15. The pH of the medium after autoclaving is 6. 9-7. 1. Any organism isolated from natural sources that gives characteristic yellow colonies in this medium under the specified conditions may presumptively be identified as either Aeromonas, Vibrio, or Pleisomonas. Aeromonas colonies are frequently surrounded by a yellow acidic halo in the green medium, and occasionally produce a small bubble of gas under the non-nutrient agar overlay. Biochemical and antigenic analyses were performed in an attempt to differentiate virulent (LD50 from one to 103 colony forming units per fish) from avirulent (LD50 greater than 106 cfu) strains of A. salmonicida. The virulent and avirulent representatives gave similar responses in the following tests: onset and duration of bacteremia in juvenile coho salmon after intramuscular injection; time of recovery of the organism from fish kidney during an acute infection; production of a leucocytolytic agent active on juvenile steelhead trout white blood cells at a dilution of 1;3000; a factor cytotoxic to cultured chinook embryo cells at a dilution of 1:320; formation of eight extracellular enzymes including elastase, several proteases and hemolysins; and the failure to produce hyaluronidase. There were two real differences observed between a virulent strain (As-SS-70) and an avirulent strain (As-Sil) of Aeromonas salmonicida. When grown on 1% peptone agar containing 1. 5% NaCl, colonies of As-SS-70 were opaque, convex, and granular while those of As-Sil were translucent, less convex, and non-granular. An attenuated strain of As-SS-70 gradually assumed avirulent colony characteristics with continued passage on artificial media. Sonicates of As-SS-70 and As-Sil were concentrated about 20 fold by dialysis against Polyethylene Glycol 4000. Use of these concentrated antigens and As-SS-70 rabbit antiserum demonstrated a unique antigen in the As-SS-70 sonicate by immunodiffusion techniques. This unique precipitin line was not observed in the As-Sil sonicate, or when anti-As-Sil rabbit antiserum was used. This was the second difference found between the virulent and avirulent strains. Groups of juvenile coho salmon were injected intramuscularly with about two LD50 doses of virulent A. salmonicida and held at temperatures of 74°F, 69°F, 64°F, 59°F, 54°F, 49°F, 44°F, and 39°F. Water temperatures of 59°F and above produced high mortality rates, with losses exceeding 40 percent at 54°F and 49°F. Mortality rates were very low at temperatures of 44°F and 39°F. The mean time to death was estimated to be 3.5 days at 69°F, with steady increases as water temperature decreased, to a maximum of 31 days at 39°F. Thus, the disease process was accelerated progressively as water temperature increased. When juvenile spring chinook salmon were intraperitoneally injected with about 1.5 LD50 doses of A. salmonicida, the mean time to death was estimated to be 2.9 days at 74°F, and was found to progressively increase as water temperature decreased, to a maximum of 18.4 days at 39°F. The effect of temperature on the growth rate of A. salmonicida in vitro appeared to be similar to its effect on the rate of progress of the infection in juvenile salmonids. The percentage of fatal infections among juvenile steelhead trout and coho salmon injected with Aeromonas hydrophila was high at temperatures of 64°F and above, moderate at 59°F, and zero at 49°F and below.
Download or read book Aeromonas Salmonicida and Aeromonas Hydrophila liquefaciens as Pathogens of Salmonid Fish A Selective Aeromonas Medium B Comparative Characteristics of Virulent and Avirulent Strains of Aeromonas Salmonicida C Effect of Water Temperature on Aeromonas Infections written by Ralph Hines MacCoy and published by . This book was released on 1982 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aeromonas Salmonicida and Aeromonas Hydrophila liquefaciens as Pathogens of Salmonid Fish written by Ralph Hines McCoy and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Temperature infectious diseases and the immune response in salmonid fish written by John L. Fryer and published by . This book was released on 1976 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Fish Pathogens written by Brian Austin and published by Springer. This book was released on 2016-10-12 with total page 761 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book puts emphasis on the isolation, taxonomy, diagnosis (phenotypic, serology and molecular biology), epizootiology, pathogenicity mechanisms, and methods of disease control (by vaccination, immunostimulation, probiotics, prebiotics, plant products, and antimicrobial compounds. Co-infections, which are attributed to more than one microbial species have been discussed. Shortcomings in knowledge have been highlighted. This sixth edition is the successor to the original version, first published in 1987, and which fills the need for an up-to-date comprehensive text on the biological aspects of the bacterial taxa which cause disease in finfish. The book is primarily targeted at researcher workers, including postgraduate students, and diagnosticians. It is anticipated that the readership will include veterinary microbiologists, public health scientists and microbial ecologists.
Download or read book Furunculosis and Other Diseases Caused by Aeromonas Salmonicida written by G. L. Bullock and published by . This book was released on 1983 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Fish Pathogens written by Brian Austin and published by . This book was released on 1987 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: De nadruk in dit werk ligt op de biologische aspecten van geteelde vis.
Download or read book Effects of Temperature on Diseases of Salmonid Fishes written by John L. Fryer and published by . This book was released on 1974 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Fish Pathogens written by B. Austin and published by Springer Science & Business Media. This book was released on 2007-11-25 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: This revised edition fills the need for an up-to-date comprehensive book on the biological aspects of the bacterial taxa which cause disease in fish. Since the 3rd edition was published in 1999, much has changed in the control of disease of farmed and wild fish. This book analyses all the new information, including that on new pathogens and new developments on long established diseases, such as furunculosis and vibriosis. Consideration is given to all of the bacterial taxa which have at some time been reported as fish pathogens, whether they are secondary invaders of already damaged tissue or serious, primary pathogens.
Download or read book Aeromonas Hydrophila and Motile Aeromonad Septicemias of Fish written by Rocco C. Cipriano and published by . This book was released on 1984 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fish Diseases written by W. Ahne and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diseases of Freshwater Fishes Caused by Bacteria of the Genera Aeromonas Pseudomonas and Vibrio written by Stanislas F. Snieszko and published by . This book was released on 1976 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book FAO Fisheries Biology Technical Paper written by and published by . This book was released on 1968 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fish Diseases and Disorders Viral bacterial and fungal infections written by P. T. K. Woo and published by . This book was released on 1995 with total page 904 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book F A O Fisheries Biology Technical Paper written by and published by . This book was released on 1968 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structure and Function of the Surface Layer of the Fish Pathogenic Bacterium Aeromonas Salmonicida written by Rafael Angel Garduño and published by . This book was released on 1993 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nordic Manual for the Surveillance and Diagnosis of Infectious Diseases in Farmed Salmonids written by Paul J. Midtlyng and published by Nordic Council of Ministers. This book was released on 2000 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
