Download or read book Advancing Cryptosporidium Parvum Detection Methodologies written by Mark C. Jenkins and published by American Water Works Association. This book was released on 2002 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.

Download or read book Cryptosporidium written by Royal Society of Chemistry (Great Britain) and published by Royal Society of Chemistry. This book was released on 2001 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive review of the current state of analytical techniques for the detection of Cryptosporidium, as well as looking at likely future developments.

Download or read book Evaluating Methodologies for Detection and Enumeration of Enteric Viruses and Cryptosporidium Parvum written by Laura Yvette Sifuentes and published by . This book was released on 2007 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular based Methods for the Detection and Quantification of Infectious Cryptosporidium Parvum Oocysts in Environmental Samples written by University of Guelph. Department of Environmental Biology and published by . This book was released on 2005 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cryptosporidium Parvum Oocysts written by Satomi Kato and published by . This book was released on 2001 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of a PCR Based Method to Detect Viable Cryptosporidium Parvum Oocysts from Environmental Water Concentrates written by Debanjana Dasgupta and published by . This book was released on 2003 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of a New Method to Detect Cryptosporidium Parvum Oocysts from Water Samples by Using Aluminium Sulphate Flocculation and Immunomagnetic Separation written by Christoph Bissegger and published by . This book was released on 1997 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Improved in Vitro Culture of Cryptosporidium parvum written by G. Widmer and published by IWA Publishing. This book was released on 2006-04-30 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this project was to investigate methods, which promote growth of Cryptosporidium (C.) parvum in culture. The researchers tested the assumption that improved survival of host cells enhances growth of C. parvum. Although certain modifications to the culture methodology improved host cell survival, enhancing host cell survival was not always accompanied by an increase in parasite density in the cell monolayer. Some cultures grown on collagen or laminin showed improved parasite growth. However, this effect was not consistent, indicating that other variables affect parasite growth. C. parvum was more prevalent in cells in the mitotic cycle than in non-dividing cells. This is consistent with the absence of certain biosynthetic pathways in C. parvum, and suggests that dividing cells may be more favorable to parasite development. Although current culture methods enable the detection of infectious Cryptosporidium oocysts, limited parasite growth reduces the sensitivity of the assay, making it necessary to apply sophisticated methods for the detection of the intracellular parasites. This study identified methods that slightly improve growth of Cryptosporidium in culture and also highlighted methods that have no effect. This report is available only as a pay-per-view item.

Download or read book Evaluation of TaqMan Real time PCR for the Detection of Viable Cryptosporidium Parvum Oocysts in Environmental Water Samples written by Melissa A. Cameron and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Cryptosporidium parvum is of growing public health concern due to its ability to survive typical water treatment processes. In order to protect the public from infection, the Environmental Protection Agency developed Method 1623 for the detection of Cryptosporidium oocysts in environmental water samples. Execution of this method is time consuming, and the results do not provide an accurate estimation of viability. Therefore, current research is focused on creating a real-time PCR method for the accurate detection of viable Cryptosporidium parvum in environmental water samples. This thesis presents the development of a real-time PCR method, and the results obtained in its use on field samples. The assay was standardized using multiple dilution series in addition to positive and negative controls. Environmental water samples were tested using this method and Method 1623 for comparison. The results were compared statistically to determine the degree of correlation between methods. The data show that the real-time PCR method correlates well to Method 1623. In addition, the assay was determined to be more cost effective and less labor intensive than Method 1623. Although these early findings are promising, additional research and development are needed before the proposed assay can be used in industry.

Download or read book Detection of Cryptosporidium Parvum Oocysts in Faeces written by Katherine A. Webster and published by . This book was released on 1996 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cryptosporidiosis and Microsporidiosis written by Franz Petry and published by Karger Medical and Scientific Publishers. This book was released on 2000-01-01 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: '... This volume provides most complete and balanced coverage of essential aspects of the pathogens as well as the diagnosis and clinical correlations of the disease they cause. It is set to become a valuable refrence for parasitologists, protozoologists, molecular biologists, clinical mecrobiologists, epidemiologists and specialists in infectious diseases.'

Download or read book Giardia and Cryptosporidium written by Guadalupe Ortega-Pierres and published by CABI. This book was released on 2009 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giardia and Cryptosporidium are both parasites of considerable global interest due to the gastrointestinal problems these organisms can cause in humans as well as domestic and wild animals. Despite its long history as the causative agent of giardiasis, the clinical and zoonotic significance of Giardia infections in humans and animals is only beginning to emerge with the application of molecular tools. Less is known about Cryptosporidium and studies seek to understand the impact of infection in livestock and other animals, its epidemiology and zoonotic significance. Presenting a comprehensive overview of recent research this book draws on the experience of experts in all fields of Giardia and Cryptosporidium research.

Download or read book Current Topics in Malaria written by Alfonso J. Rodriguez-Morales and published by . This book was released on 2016-11-30 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Indicators for Waterborne Pathogens written by National Research Council and published by National Academies Press. This book was released on 2004-06-19 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â

Download or read book Cryptosporidium written by Jan R. Mead and published by Humana. This book was released on 2019-08-27 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon. The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Download or read book Technological Innovations in Sensing and Detection of Chemical Biological Radiological Nuclear Threats and Ecological Terrorism written by Ashok Vaseashta and published by Springer Science & Business Media. This book was released on 2012-01-03 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book arises from the NATO Advanced Study Institute “Technological Innovations in Detection and Sensing of CBRN Agents and Ecological Terrorism” held in Chisinau, Republic of Moldova in June 2010. It comprises a variety of invited contributions by highly experienced educators, scientists, and industrialists, and is structured to cover important aspects of the field that include developments in chemical-biological, and radiation sensing, synthesis and processing of sensors, and applications of sensors in detecting/monitoring contaminants introduced/dispersed inadvertently or intentionally in air, water, and food supplies. The book emphasizes nanomaterials and nanotechnology based sensing and also includes a section on sensing and detection technologies that can be applied to information security. Finally, it examines regional, national, and international policies and ethics related to nanomaterials and sensing. It will be of considerable interest and value to those already pursuing or considering careers in the field of nanostructured materials and nanotechnology based sensing, In general, it serves as a valuable source of information for those interested in how nanomaterials and nanotechnologies are advancing the field of sensing, detection, and remediation, policy makers, and commanders in the field.

Download or read book Advanced Flow Cytometry Applications in Biological Research written by R.C. Sobti and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.