Download or read book Advances in GAPDH Protein Analysis A Functional and Biochemical Approach written by Shanmugasundaram Ganapathy-Kanniappan and published by Springer. This book was released on 2018-01-16 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The distinguishing feature of the book is that it covers, in addition to other methods, some of the uncommon but valuable techniques as well. For example, in-gel visualization of enzyme activity, immunoblotting protocols for native (non-denatured) proteins, and proteins resolved by pH-gradient [IEF-isoelectrofocusing], etc. These expedient methods are relevant and vital for the verification of biochemical properties of GAPDH, or similar protein of interest. This work outlines detailed protocols that are essential to investigate classical (cellular) and recently reported extracellular (secretory) isoforms of GAPDH. Precisely, the book covers techniques pertinent to enzymatic and non-enzymatic analysis of GAPDH that include, but not limited to, electrophoretic mobility shift assay (EMSA), two-dimensional (2D)-immunoblotting, immunofluorescence/confocal microscopy, mass spectrometry, ion-exchange and affinity chromatography. Readers will discover the importance of the experimental methods described in the book as they relate to the evaluation of the role and significance of GAPDH. Furthermore, majority of the methods described in the book have also been validated in the author’s laboratory, besides other research groups worldwide, underlining the repeatability and reproducibility of the protocols. Each method begins with an abstract and a brief background emphasizing its application and relevance. This will enable the readers to determine the choice of experimental design according to their research objectives. The book explains the methods systematically with ample illustrations to facilitate quick and easy comprehension of the practical knowledge. Although the book is focused on GAPDH, many of the protocols may be adopted to other proteins or enzymes with minimal modifications. Noteworthy, it is unequivocally established that GAPDH is a multifunctional protein involved in several cellular processes of health & disease conditions. Hence, this book will be a valuable practical guide for young researchers, scientists and clinician-scientists.

Download or read book Advances in malignant pleural mesothelioma Diagnosis treatment and molecular mechanisms written by Glen Reid and published by Frontiers Media SA. This book was released on 2023-03-24 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book GAPDH Biological Properties and Diversity written by Norbert W. Seidler and published by Springer Science & Business Media. This book was released on 2012-07-31 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book represents a comprehensive review and synthesis of the biomedical literature that spans over a half-century on a single protein called glyceraldehyde 3-phosphate dehydrogenase (or, GAPDH). Due to the protein’s involvement in a vast array of cellular activities, GAPDH is of interest to the cell biologist, immunologist, virologist, biochemist etc. The protein has a significant role in fertility, cancer and neurodegeneration, suggesting that this book can be a vital resource for drug development. GAPDH function may provide insight into anesthesia. Furthermore, GAPDH is highly conserved meaning that the protein found in microorganisms, such as pathogens, remained relatively unchanged in evolution. Pathogens use GAPDH as a virulence factor, offering a unique challenge in developing anti-microbial agents that target this protein. To the evolutionary biologist, a book on the multi-functionality of GAPDH provides a focal point for a cogent discussion on the very origin of life.

Download or read book Advances in Multi omics Study of Filamentous Plant Pathogens written by Danyu Shen and published by Frontiers Media SA. This book was released on 2022-09-22 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in Molecular and Pharmacological Mechanisms of Novel Targeted Therapies for Melanoma written by Luana Mota Ferreira and published by Frontiers Media SA. This book was released on 2024-04-15 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malignant melanoma is the most aggressive type of skin cancer with invasive growth patterns. Recent advances in melanoma management have focused on cell growth, proliferation, migration, and survival biomarkers. Numerous FDA-approved molecular-targeted therapies, such as tyrosine kinase inhibitors (TKIs), have been developed targeting genetic biomarkers that play a critical role in tumorigenesis when overexpressed. The use of targeted therapies as an alternative or adjuvant to immunotherapy has revolutionized the management of metastatic melanoma. Although this treatment strategy is more efficacious and less toxic than traditional therapies, targeted therapies are less effective after prolonged treatment due to acquired resistance given the mutations and activation of resistant mechanisms in melanoma tumors. In this sense, identifying novel target therapies and repositioned drugs that could modulate the disease progression, the potential molecular and pharmacological mechanisms of therapies are of utmost interest.

Download or read book Research Advances in Gestational Diabetes Mellitus Neonatal Diabetes Mellitus and Metabolic Disorders written by Ihtisham Bukhari and published by Frontiers Media SA. This book was released on 2022-09-05 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic advancements for improving the plant tolerance to biotic and abiotic stresses written by Krishnanand P. Kulkarni and published by Frontiers Media SA. This book was released on 2024-05-31 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crop plants are constantly exposed to multiple abiotic (such as drought, salinity, cold, flooding, heavy metal, and heat) and/or biotic (bacterial/fungal/viral) stress factors that hinder their growth and development, subsequently leading to decreases in quality and yield. During the last two decades, many classical genetic and breeding approaches have been used to develop stress-tolerant and climate-adaptable plants that can provide a better yield to meet food demands. Climate change poses a major risk to food security as the world faces frequent floods, droughts, heat waves, and the emergence of new invasive pests and diseases. Novel genomic and genetic approaches look promising to improve plant resilience under stress conditions and achieve sustainable crop improvements. Recent advances in sequencing technologies have facilitated the generation of a plethora of genomic resources in a variety of crop and plant species. With the increased availability of genomic and transcriptomic data, an increasing number of quantitative trait loci and candidate genes are being identified for their application in improving plant tolerance to abiotic and biotic stresses. New approaches such as genomic selection and genomic-assisted breeding have been utilized to develop stress-tolerant cultivars in a variety of plant species. Furthermore, transgenics and rapidly evolving CRISPR technology offer great potential for plant improvement. This Research Topic aims to provide insights into the molecular and genetic factors involved in imparting abiotic and biotic stress tolerance in plants and their application in enhancing plant adaptation to these stress conditions. To review the progress in this research category, we invite manuscripts related to the plant responses to abiotic/biotic stresses and trait improvement through genomic selection, and transgenic or gene-editing approaches. Studies including physiological, biochemical, and molecular genetic analyses revealing the mechanisms involved in plant response to abiotic/biotic stresses are welcome. Topic editor Dr. Balaji Aravindhan Pandian is employed by Enko Chem Inc. All other Topic Editors declare no competing interests with regard to the Research Topic subject.

Download or read book Luminescent Proteins Advances in Research and Application 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: Luminescent Proteins—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Luciferases. The editors have built Luminescent Proteins—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Luciferases in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Luminescent Proteins—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book Advances in emerging coronavirus identification and tracing methods written by Junping Peng and published by Frontiers Media SA. This book was released on 2023-05-25 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in Genetic and Epigenetic Mechanisms of Therapeutic Resistance in Cancer written by Yilin Zhang and published by Frontiers Media SA. This book was released on 2023-07-03 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Analysis and Purification written by I.M. Rosenberg and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.

Download or read book Advances of endocrine and metabolic cardiovascular outcomes From basic to clinical science volume II written by Si Jin and published by Frontiers Media SA. This book was released on 2023-02-15 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book In Vitro Fertilization written by Zsolt Peter Nagy and published by Springer. This book was released on 2019-07-03 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.

Download or read book Understanding the Immuno Oncological Mechanism of Cancer Using Systems Immunology Approaches written by Rifat Hamoudi and published by Frontiers Media SA. This book was released on 2024-01-02 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Current Advances in Protein Biochemistry written by and published by . This book was released on 1997 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in the Molecular Mechanisms in Gastrointestinal Tumorigenesis and Treatment written by Ruowen Zhang and published by Frontiers Media SA. This book was released on 2024-09-20 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gastrointestinal (GI) cancer, the group of cancers that affect the gastrointestinal tract and other organs that are contained within the digestive system, including the esophagus, pancreas, stomach, colon, rectum, anus, liver, biliary system and small intestine, is the most common malignant diseases with high mortality rates, leading to a substantial burden on public health and healthcare systems around the world. Diverse subtypes of GI cancer impressively exhibit similar characteristics like high frequency oncogenic mutation burden, early metastatic potency, chemo-resistance and immunotherapeutic tolerance indicating a possible unique tumorigenic mechanism.

Download or read book Advanced Molecular Targets in the Diagnosis and Treatment of Gastrointestinal Cancers written by Zsolt Kovacs and published by Frontiers Media SA. This book was released on 2023-06-05 with total page 682 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gastrointestinal cancers are among the most common cancer types, based on the Cancer Genome Atlas. GI cancers are within the most frequent malignancy, with almost 150.000 new cases in 2020. On one hand a big number of researches are focused on the diagnosis, new diagnostic approaches in upper and lower gastrointestinal tract cancers. On the other hand in the last 10 years several papers had been published about the possible therapeutic targets, pointing to precision and personalized medicine.