Download or read book Adsorption of Thermomonospora Fusca E3 and E5 and Trichoderma Reesei CBHI Cellulases on Cellulose and Silica written by Worakrit Suvajittanont and published by . This book was released on 1999 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Adsorption of Trichoderma Reesei CBHI and Thermomonospora Fusca E5 Cellulases on Model Solid Surfaces written by Carolyn S. Baker and published by . This book was released on 1998 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this research, the interfacial behavior of Trichoderma reesei CBHI and Thermomonospora fusca E5 cellulases were studied at synthetic surfaces. For this purpose, colloidal silica and polystyrene particles were used to prepare cellulase-particle suspensions that were analyzed by several solution-phase techniques. These included circular dichroism spectroscopy, size exclusion chromatography and filtration, and a spectrophotometric assay for cellulase activity. All techniques were performed in the presence and absence of particles. Circular dichroism spectroscopy (CD) and size exclusion chromatography showed, however, that binding did not occur between either cellulase and silica, presumably because silica is hydrophilic and negatively charged. Binding did occur between each cellulase and polystyrene, most likely mediated through hydrophobic associations. Cellulase-polystyrene complexes were not analyzed using CD because of high light absorption by the polystyrene nanoparticles. Upon adsorption to polystyrene, the activity of the E5 dropped about 95% relative to that of the free enzyme. While this substantial loss in activity may have been the result of binding being mediated through the catalytic domain, strong evidence supporting the thought that adsorption occurs through hydrophobic associations, mediated through the binding domain, suggests that structural or steric factors were partly responsible for the loss.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1999 with total page 898 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applied and Environmental Microbiology written by and published by . This book was released on 2007 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Adsorption and Synergism of Cellulases During Hydrolysis of Cellulosic Materials written by Hanshu Ding and published by . This book was released on 2000 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization and Adsorption of the Cellulase Components from Trichoderma Reesei written by Andreas Kyriacou and published by . This book was released on 1987 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Light microscopy and monitoring of sugar production during cellulose hydrolysis indicated that conditions which limit predominance in adsorption by any one of the cellulase components, enhance synergism and increase degree of hydrolysis." --
Download or read book Degradation of Cellulose by Cellulases from Trichoderma Reesei written by Bernd Nidetzky and published by . This book was released on 1992 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mode of Action of Trichoderma Reesei Cellobiohydrolase I on Crystalline Cellulose written by Malee Srisodsuk and published by . This book was released on 1994 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulose Hydrolysis by Trichoderma Reesei Cellulases written by József Medve and published by . This book was released on 1997 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Adsorption of Cellulase from Trichoderma Reesei en Wheat Straw written by and published by . This book was released on 1988 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Adsorption desorption of Cellulases from Cellulose and Their Characterization by HPLC written by Donald Edwin Otter and published by . This book was released on 1987 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Cellulase Synthesis in Thermomonospora Fusca and Cloning Sequencing and Expression of an Alkaline Serine Protease Gene in Thermomonospora Fusca written by Guifang Lao and published by . This book was released on 1996 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Relative Activity of the Cellulase Enzyme System of Trichoderma Reesei with Native and Modified Cellulosic Substrates written by Ean-Tun Liaw and published by . This book was released on 1994 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Collection of Trichoderma Reesei Cellulase by Foaming written by Qin Zhang and published by . This book was released on 2007 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Facing the current energy crisis, people try to find alternative energy sources. Certain microbes can ferment soluble sugars to ethanol, which is a well known biofuel. Cellulosic biomass is the most abundant renewable resource. Cellulose can be broken down to soluble sugars by the cellulase enzymes produced only by some microbes. The combination of the above two processes, i.e., break-down of cellulose to sugars and conversion of sugars to ethanol, represents a major approach of solving the energy crisis through renewable biological processes. One of the bottle necks of this approach is the relatively high production cost of cellulase. In this research, the main task was to develop a separation process for cellulase using cost-effective and environment-friendly foam-based techniques. First, the foaming properties of the fermentation broth of Trichoderma reesei RUT C-30 were investigated. The experiment results showed that cellulase was not the dominant substance that caused the foaming of the fermentation broth, although the increase of cellulase concentration in the fermentation process appeared to parallel the increase in foaming tendency. Cellulase concentration was found to be only a weakly positive factor contributing to the foaming whereas the presence of cells and solid cellulose powders had negative effects on foaming. The cell wall was found to be hydrophobic according to the study conducted to observe the cells partition between hydrophobic (organic solvent: n-hexadecane) phase and hydrophilic (aqueous) phase. The finding of hydrophobic cell wall was consistent with the observation that the cells would come out with foam in the foaming study. To prevent the cell from being foamed out of the fermentor during the foaming process intended for cellulase collection, the cell immobilization in small (3mm*3mm*3mm) pieces of polyurethane (PU) foams with different porosity and pore size was also investigated. With some of the PU foam materials, the cells could be effectively immobilized and would not be foamed out during the foaming process. Nevertheless, this system did not function well in the typical stir-tank fermentor: the high shear stress present near the tip of the impeller blades appeared to strip the immobilized cells off the PU foam pellets and caused significant subsequent growth of free (not immobilized) cells in the broth. In a subsequent study of cellulase production using cellulose hydrolysate (CH) as the inducing substrate, it was observed that the addition of the hydrolysate resulted in higher enrichment ratios of cellulase concentration in the collected foamate (over the residual concentration in the spent broth). The foaming study was then expanded to include the addition of other cellulase substrates and substrate analogs, such as xylan hydrolysate (XH) and carboxylmethylcellulose (CMC). The results clearly demonstrated the addition of CH, XH and CMC would improve the enrichment and purification of cellulases by foaming. The improvements were attributed to the affinity binding between the substrates/substrate analogs and the cellulase enzymes and the increased partition of the substrate-enzyme complexes to the foam's air-liquid interfaces. To further pursue the above affinity foaming phenomenon for significantly improved collection and, potentially, purification of cellulase, a polymeric foaming agent was designed and synthesized. The polymeric surfactant has two parts, one is the hydrophobic region containing methylmethacrylate (MMA) and methacrylic acid (MAA), the other part is cellobiose, i.e., the substrate of the enzyme to bind with during foaming process. Unfortunately, the foaming ability of this synthetic surfactant was not good enough and its synthesis was complicated. As an easier first step, two glycolipid biosurfactants, sophorolipids and rhamnolipids, were investigated as the possible affinity foaming agents. Both glycolipids had a disaccharide moiety that was expected to bind selectively to the â-glucosidase in cellulase. While sophorolipids did not foam well, rhamnolipids showed great foaming ability and selectivity towards â-glucosidase. The enrichment ratio of â-glucosidase activity in the foamate reached up to 20 in the solutions prepared by cellulase powders (from Sigma). The significant benefits of such affinity foam collection and/or fractionation techniques were clearly shown."--Abstract.
Download or read book Current Developments in Biotechnology and Bioengineering written by Ashok Pandey and published by Elsevier. This book was released on 2016-09-17 with total page 888 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for isolating and purifying them from a production medium. During the last few years, the tools of molecular biology and genetic and metabolic engineering have rendered tremendous improvements in the production of industrial products by fermentation. Structured by industrial product classifications, this book provides an overview of the current practice, status, and future potential for the production of these agents, along with reviews of the industrial scenario relating to their production. - Provides information on industrial bioprocesses for the production of microbial products by fermentation - Includes separation and purification processes of fermentation products - Presents economic and feasibility assessments of the various processes and their scaling up - Links biotechnology and bioengineering for industrial process development
Download or read book Adsorption on Silica Surfaces written by Eugene Papirer and published by CRC Press. This book was released on 2000-04-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Progresses from theoretical issues to applications. Contains a historical overview, in-depth considerations of various scenarios of silica adsorption, and results from the latest research. Invaluable for broad coverage of the expanding field of silica research."
Download or read book Secretions and Exudates in Biological Systems written by Jorge M. Vivanco and published by Springer Science & Business Media. This book was released on 2012-01-21 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Secretions and emissions in biological systems play important signaling roles within the organism but also in its communications with the surrounding environment. This volume brings together state-of-the-art information on the role of secretions and emissions in different organs and organisms ranging from flowers and roots of plants to nematodes and human organs. The plant chapters relate information regarding the biochemistry of flower volatiles and root exudates, and their role in attracting pollinators and soil microbial communities respectively. Microbial chapters explain the biochemistry and ecology of quorum sensing and how microbial communities highly co-adapted to plants can aid in bio-energy applications by degrading ligno-cellulosic materials. Other chapters explain the biology of secretions by nematodes, algae and humans, among other organisms. This volume will be a welcome addition to the literature, as no other book covers aspects related to biological secretion in such a holistic and integrative manner.
