Download or read book Adhesion Protein Protocols written by Amanda S. Coutts and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Download or read book Methods in Molecular Biology Adhesion protein protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Adhesion Protein Protocols written by Elisabetta Dejana and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adhesion molecules are of fundamental importance in the regulation of immunity, inflammation, tissue remodeling, and embryonic development. They comprise different families of homologous proteins, such as selectins, integrins, cadherins, and immunoglobins. In addition, beyond these groups, other str- tures with adhesive properties, such as proteoglycans, occludin, and CD44, have been characterized recently. An understanding of the type and characteristics of adhesive molecules expressed by the different cell types and the possibility of manipulating their activity promises considerable clinical potential. Antibodies, small peptidic and nonpeptidic molecules, have recently been used to inhibit thrombosis by blocking platelet aggregation or inflammation through inhibition of leukocyte infiltration and adhesion. Inhibitors of adhesive molecules are used in expe- mental systems for the study of tumor growth and dissemination. Among major goals in the field are the identification of new members of the known adhesive protein families and of independent new adhesive structures. After structural characterization, even more demanding is the study of the biological activity of the new proteins, and the development of simple, rapid tests for the screening of possible inhibitors. In this regard, the production of such reagents as fragments and antibodies would help define the structure–function relati- ship of individual proteins. Data available in the literature show the complexity of the adhesive process and how different molecular epitopes might contribute to the adhesive properties of a single structure. Finally, a new area of investi- tion is the characterization of the intracellular signaling cascade triggered by the engagement of transmembrane adhesive proteins.
Download or read book Integrin and Cell Adhesion Molecules written by Motomu Shimaoka and published by Humana Press. This book was released on 2011-09-15 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.
Download or read book Signaling Through Cell Adhesion Molecules written by Jun-Lin Guan and published by CRC Press. This book was released on 2019-04-30 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of signal transduction research is one of the fastest growing in all of biomedical research in recent years. Signaling through cell adhesion molecules have long been of interest because of their importance in embryonic development, homeostasis, immune responses, wound healing , and malignant transformation. However, it is only recently re
Download or read book Extracellular Matrix Protocols written by Charles Streuli and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix. In ad- tion to its conventional role in providing a scaffold for building tissues, the extracellular matrix acts as a directional highway for cellular movement and provides instructional information for promoting survival, proliferation, and differentiation. Indeed, the extracellular matrix is beginning to take a starring role in the choreography of cell and tissue function. The diverse roles of the extracellular matrix are reflected in its highly complicated structure, consisting of an ever increasing number of components. Yet the mechanisms of extracellular matrix assembly and how they influences cell behavior are only just beginning to be understood. In order to solve these problems new methodologies are, of necessity, being developed. Many of these technologies are highly sophisticated and are currently available only in a ha- ful of laboratories. However, we believe that they can readily be transported and established by other researchers. Thus, the purpose of Extracellular Matrix Protocols is to present some of these complicated techniques in a style that is relatively easy to reproduce.
Download or read book Protein Complex Assembly written by Joseph A. Marsh and published by Humana. This book was released on 2019-04-15 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Adhesion Protein Protocols written by Amanda S. Coutts and published by Humana. This book was released on 2007-02-26 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Download or read book Integrin Interactome written by Miguel Vicente-Manzanares and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Measurement of Cellular Adhesions and Adhesion Protein Dynamics Using Tracking Paired with Spatio temporal Image Correlation Spectroscopy written by Christopher Kicak-Deroy and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Proteins are ubiquitous in biological systems and while much is known about protein structure, less is known about the movement of these proteins. The phenomenon of movement also occurs at the cellular level through cell migration and, in particular, cells depend upon the movement of proteins to enable cell motility. More precisely, cell migration is dependent upon cytoskeletal structures, including focal adhesions, complexes which include multiple proteins and enable cells to exert forces upon the underlying substrate and migrate. The basic structure and components of the cytoskeleton are fairly well known, yet much less is known about their dynamic assembly and disassembly. Motile cells are known to be involved in numerous biological processes and thus studying the flow of proteins involved in cell migration has the potential to clarify their roles and lead to a more advanced understanding of cell migration. Major protein components that play a role in the formation of focal adhesions have been identified. Using genetically engineered fluorescent variants of these proteins, we can image cells expressing fluorescently-tagged proteins via fluorescence microscopy, and thereby obtain quantitative results on the location and movement of key proteins of interest in migrating cells. In this work, a correlation analysis was performed on the measured fluorescence fluctuations within image series in order to determine the magnitude and direction of protein flows within sub-regions of migrating cells. The correlation analysis technique known as STICS (spatiotemporal image correlation spectroscopy) was utilized and accomplishes this by using the full spatiotemporal correlation function. It is important that fluorescence variations in space throughout the cell as well as variations through time be investigated in the image series. STICS is well suited for this as it provides vector map image series of the fluorescent protein flows. A principal aspect of this thesis is performing the STICS correlation analysis on image series of cells containing fluorescently-tagged versions of 5 key adhesion proteins with cells on substrates of different rigidities. Another principal aspect is to track and measure the development of adhesions simultaneously with the STICS analysis of protein flows that take part in focal adhesion development. In order to designate the STICS flows detected in the neighborhood of adhesions to specific adhesions correctly, image processing methods were employed including filtering of noise in space and in time, adhesion segmentation and adhesions tracking. Image series were treated for noise sources with image filtering in the spatial domain to remove background noise and in the temporal domain using a Butterworth filter to remove lower frequencies that obscure the signal of interest from adhesion protein populations exhibiting directed flow. For a large number of focal adhesions, local flows were obtained throughout trajectories using STICS correlation analysis of fluorescence fluctuations, while also measuring the physical properties of the focal adhesions. The adhesion analysis protocol developed for this thesis tracks all adhesions detected from the cell's expressed fluorescent proteins, and provides a neighbourhood STICS flow at each frame for tracked adhesions along their trajectories. As well, physical properties including adhesion area, major axis length, and adhesion speeds are obtained for each frame along the trajectories of tracked adhesions. Distributions of adhesion physical properties and local protein flow speeds were obtained for adhesions across multiple NIH3T3 cells for five adhesion proteins: paxillin, vinculin, talin, actin, and [alpha]-actinin. A substrate most resembling glass was first used, followed by a substrate with a greater concentration of the endogenous fibronectin to decrease substrate rigidity. This is not the full abstract." --
Download or read book The Integrin Interactome written by Miguel Vicente-Manzanares and published by Humana. This book was released on 2020-11-20 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
Download or read book Mechanobiology written by Ronen Zaidel-Bar and published by Springer Nature. This book was released on 2023-01-01 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mechano-responses at various scales, to measure forces applied by cells on the extracellular matrix, as well as chapters on force measurement inside cells, probing cell signaling and gene expression in response to force, and biophysical modeling of cell shape and protein dynamics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mechanobiology: Methods and Protocols aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynamics from a mechanobiological perspective, molecular biologists interested in the effects of force on proteins, as well as for cancer biologists and biophysicists.
Download or read book Signaling Through Cell Adhesion Molecules written by Jun-Lin Guan and published by CRC Press. This book was released on 2019-04-30 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of signal transduction research is one of the fastest growing in all of biomedical research in recent years. Signaling through cell adhesion molecules have long been of interest because of their importance in embryonic development, homeostasis, immune responses, wound healing , and malignant transformation. However, it is only recently re
Download or read book Adhesion of Cells Viruses and Nanoparticles written by Kevin Kendall and published by Springer Science & Business Media. This book was released on 2010-11-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Adhesion of Cells, Viruses and Nanoparticles" describes the adhesion of cells, viruses and nanoparticles starting from the basic principles of adhesion science, familiar to postgraduates, and leading on to recent research results. The underlying theory is that of van der Waals forces acting between cells and substrates, embodied in the molecules lying at the surfaces, together with the geometry and elasticity of the materials involved. The first part describes the fundamental background to adhesion principles, including the phenomenology, the important equations and the modeling ideas. Then the mechanisms of adhesion are explored in the second part, including the elastic deformations of spheres and the importance of the energy of adhesion as measured in various tests. It is demonstrated that adhesion of cells is statistical and depends on Brownian movement and on the complex multiple contacts that can form as cells move around. Then, detailed chapters on cell adhesion, contact of viruses and aggregation of nanoparticles follow in Part 3. Finally, the last chapter looks to the future understanding of cell adhesion and points out some interesting directions of research, development and treatment of diseases related to these phenomena. This book is an ideal resource for researchers on adhesion molecules, receptors, cell and tissue culturing, virus infection, toxicity of nanoparticles and bioreactor fouling. It can also be used to support undergraduate and Masters level teaching courses. "This is a fascinating book and it is an invaluable resource for understanding particle-particle/surface adhesion at micro- and nano- scales. I intend to keep one for my future reference and highly recommend it to my students." (Prof. Zhibing Zhang, School of Chemical Engineering, University of Birmingham, UK)
Download or read book Application of Solution Protein Chemistry to Biotechnology written by Roger L. Lundblad and published by CRC Press. This book was released on 2009-05-12 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the versatility of the author's science and the depth of his experience, Application of Solution Protein Chemistry to Biotechnology explores key contributions that protein scientists can make in the development of products that are both important and commercially viable, and provides them with tools and information required for successfu
Download or read book PCR in Bioanalysis written by Stephen J Meltzer and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Download or read book Neutrophil Methods and Protocols written by Mark T. Quinn and published by Springer Science & Business Media. This book was released on 2007-08-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.
