Download or read book A Study of Gene Expression in Bacillus Subtilis written by Yi-Wen Chen Chiang and published by . This book was released on 1984 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Single Cell Gene Expression in Bacillus Subtilis written by John David Chung and published by . This book was released on 1994 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics and Biotechnology of Bacilli written by Mark M. Zukowski and published by Elsevier. This book was released on 2012-12-02 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics and Biotechnology of Bacilli, Volume 3 covers the proceedings of the Fifth International Conference on Genetics and Biotechnology of Bacilli, held on July 9-12, 1989 at the Asilomar Conference Center, Pacific Grove, California. It summarizes the remarkable progress made in the genetics and biotechnology fields of Bacilli. It is organized into four parts, encompassing 43 chapters, which focus on gene regulation and structure, enzyme structure, Bacillus thuringiensis toxins, and stationary phase gene regulation. Part I covers topics related to gene regulation and structure of Bacilli, such as control of gene expression, mutation, genetic organization, DNA sequence analysis, and identification of transcript units. It also discusses gene replication in Bacillus subtilis plasmids, levanase operon of B. subtilis, and characterization of global regulon in B. subtilis. The next part of this book focuses on the structure of various enzymes found in B. subtilis, including alpha amylases, subtilisin, alkaline phosphatase, and levansucrase. Part III discusses the generation of functional B. thuringiensis toxin hybrid genes, regulation of crystal protein gene promoters, toxicity of B. thuringiensis delta-endotoxin, and insecticidal activity of chimeric protoxins. The concluding part covers the aspects of signal transduction, regulation of differential gene expression during B. subtilis sporulation, and gene cloning and deletion for extracellular proteases of B. subtilis. It also discusses genetic and biochemical aspects of protein phosphorylation; properties of B. subtilis spores; control of stationary phase gene expression; and the novel regulatory gene, senS, of B. subtilis. This book is a valuable source of information for microbiologists, research biologists, and Bacilli enthusiasts.

Download or read book Regulation of Gene Expression in Stationary Phase of Bacillus Subtilis written by Soon-Youl Lee and published by . This book was released on 1995 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Cloning and Gene Regulation in Bacilli written by A Ganesan and published by Elsevier. This book was released on 2012-12-02 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.

Download or read book Study of Gene Expression Patterns in Bacillus Subtilis DegQ Single Mutant and the DegUQ Double Mutant Using DNA Microarrays written by Priya S. Iyer and published by . This book was released on 2005 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Gene Expression in Stationary Phase of Bacillus Subtilis written by Ann Rutherford Redfield and published by . This book was released on 1997 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics and Biotechnology of Bacilli Volume 2 written by A.T. Ganesan and published by Elsevier. This book was released on 2012-12-02 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics and Biotechnology of Bacilli, Volume 2 is a collection of papers from the "Fourth International Conference on Bacilli" held in California on June 21-24, 1987. One paper reviews the results of cloning and characterization of genes for secreted enzyme and of genes that control the expression of secreted enzymes in relation with other prokaryotic regulatory systems. Other papers tackle the regulation of gene expression during sporulation, the sigma factors, bacterial toxins, and antibiotic resistance genes. One paper reports that three genes responsible for the code for peptides found in BT cuboidal crystals have been successfully cloned. The three codes have different toxic characteristics in relation to tobacco hornworm larvae or mosquito larvae. Other papers examine replication. Such examination pertains to the two levels of control on the chromosome involved in DNA replication, or to the possible functional importance that several membranes associated DNA subcomplexes can have in Bacillus subtilis, where one of these appear to control initiation. Other papers discus secretion and extracellular enzymes, as well as, the different genetic systems and methods. This collection can prove beneficial for biochemists, micro-biologists, cellular researchers, and academicians involved in the study of cellular biology, microchemistry, or toxicology.

Download or read book Determinants of Compartmentalization of Gene Expression During Sporulation in Bacillus Subtilis written by Francis Anthony Di Donato and published by . This book was released on 2008 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacillus subtilis, a benign gram-positive bacterium, utilizes the strategy of sporulation, which enables it to survive stresses such as starvation, desiccation, and UV irradiation. The spore provides greatly heightened safety to heat and noxious chemicals and remains dormant until conditions become favorable to growth. Sporulation by Bacillus subtilis is a primitive example of cell differentiation. The study of sporulation by Bacillus subtilis has become a paradigm for the study of differentiation in prokaryotes. Central to this process is the establishment of distinct patterns of gene expression in the cell types involved. Our laboratory has developed a two-part sacB/SacY probe to study the temporal and spatial compartmentalization of gene expression. It utilizes the anti-terminator protein SacY to control the transcription of reporter lacZ, (cloned downstream of the sacB gene,) which is regulated by anti-termination. Expression of sacB and SacY is regulated by a pair of promoters specific for sF (prespore specific) and sE (mother cell specific.) Both SacY and sacB must be in the same compartment of the sporulating cell in order to obtain ß-galactosidase activity. Mutagenesis of Bacillus subtilis was employed to identify determinants of compartmentalization of gene expression during sporulation. Mutants were screened for loss of compartmentalization using the two-part probe. In addition to the two-part sacB/SacY probe, a second method was developed; transposon mutagenesis was performed on strains where expression of gfp was regulated by promoters recognized by either sF or sE. Cells deficient in sporulation were isolated and evaluated by fluorescence microscopy for uncompartmentalized gfp expression. A rescue vector was developed that allowed for efficient cloning of Tn10 insertions. This plamid, pJP17, proved to be an essential tool. Mutations causing uncompartmentalized sF activity were identified in spoIIIE, spoIIIAA, spoIIIAB, spoIIIJ, spoIIE, spoIIAA, spoIID, spoIIM, kinA and ald. The spoIIIE mutation provides the most dramatic phenotype, and was the only mutation, that resulted in 100% loss of compartmentalization during stage II of sporulation. In contrast to all other mutants, the dramatic stage II loss of compartmentalized activity of sF indicates a regulatory role for SpoIIIE, which has yet to be elucidated. Taken together, these results indicate a central role for SpoIIIE in preventing activation of sF in the mother cell in addition to its DNA translocation activity.

Download or read book The Regulation of Gene Expression During Sporulation in Bacillus Subtilis written by Raymond Schuch and published by . This book was released on 1995 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Use of the Transposon Tn917 to Study Temporally Regulated Gene Expression in Bacillus Subtilis written by Kathleen Marie Sandman and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Gene Expression by Iron in Bacillus Subtilis written by Qian Chen and published by . This book was released on 1996 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bacillus Subtilis written by David Dubnau and published by Elsevier. This book was released on 2012-12-02 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Molecular Biology of the Bacilli, Volume I: Bacillus subtilis focuses on areas of research traditionally investigated in Bacillus subtilis, as well as topics in which outstanding progress has been made. It discusses the sporulation, defective bacteriophage, and transformation of Bacillus subtilis. Organized into 11 chapters, the book begins with the genetic map of Bacillus subtilis, followed by DNA replication and RNA polymerase of the said species. The book then describes the translational apparatus of Bacillus subtilis. It also explains the genetic transformation in Bacillus subtilis; the sporulation genes; the regulatory mechanisms in the development of lytic bacteriophages in this species; the temperate Bacillus subtilis phages; the specialized transduction in Bacillus subtilis; and molecular cloning in this organism. Lastly, the book considers the most economically important areas of the microbiological industry employing bacilli, including the production of enzymes, nucleosides, riboflavin, and preparations pathogenic to insects. This book will be useful to scientists who are concerned with the use of Bacillus subtilis as a tool for the study of molecular biology and to those who wish to increase the medical, veterinary, and industrial usefulness of this and related organisms.

Download or read book Bacillus Molecular Genetics and Biotechnology Applications written by A.T. Ganesan and published by Academic Press. This book was released on 2012-12-02 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacillus Molecular Genetics and Biotechnology Applications contains the proceedings of the Third International Conference on the Genetics and Biotechnology of Bacilli, held at Stanford University in Stanford, California on July 15-17, 1985. Contributors discuss the progress that has been made concerning the molecular genetics and biotechnology of Bacillus and cover topics related to transposons and plasmids, secretion, gene cloning, and gene expression. This volume is organized into five sections encompassing 39 chapters and begins with an overview of the origin and the state of molecular genetics, along with some of the contributions microbiology has made to fundamental biology. It then emphasizes the importance of scientifically based regulatory decisions and responsible industry actions for effective biotechnology regulation. The chapters that follow focus on Bacillus subtilis, touching on topics such as DNA recombination in plasmids, genetic system for stabilizing cloned genes, regulation of sporulation, and non-complementing diploids. The reader is methodically introduced to the secretion and maturation of subtilisin, cloning in streptomycetes, and genetic exchange and prospects for cloning in Clostridia. The book concludes with a chapter that describes an integrative and amplifiable secretion vector using the inducible promoter and signal peptide from the B. subtilis levansucrase (LS) gene. This book will be of interest to geneticists, microbiologists, and biotechnologists, as well as students and researchers in the fields of molecular biology and biochemistry.

Download or read book Developmental Gene Expression Regulated by a Cascade of Sigma Factors in Bacillus Subtilis written by Bin Zhang and published by . This book was released on 1997 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Regulation of Gene Expression During Sporulation of Bacillus Subtilis written by Edward Ivan Amaya and published by . This book was released on 2000 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Signals Switches Regulons and Cascades written by Society for General Microbiology. Symposium and published by Cambridge University Press. This book was released on 2002-04-18 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher Description