Download or read book A Structure Function Toolbox for Membrane Transporter and Channels written by and published by Academic Press. This book was released on 2017-08-17 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594, a new release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers membrane transporter and channels
Download or read book Membrane Transporters and Channels as Targets for Drugs written by Graça Soveral and published by Frontiers Media SA. This book was released on 2020-01-03 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transporters and channels are membrane proteins that mediate the traffic of metabolites, water and ions across biological membranes. Membrane transport proteins are crucial to maintain homeostasis and assure cell survival upon intracellular or environmental stress. A failure of any of these transport systems may have dramatic consequences for cell function. There is increasing evidence that membrane transport proteins play important functions in healthy conditions and that their absence or dysfunction may cause diseases. In recent years much attention has been paid to diseases resulting from defective transporters (“carrier diseases”) and ion channels (“channelopathies”). Very interestingly, altered expression of transporters has been described in several human pathologies. On this basis, many transport proteins are well acknowledged targets for drugs. Many others are involved in drug delivery and disposition and/or are considered potential targets. Others are off-targets for drugs and then, are responsible for side effects. Thus, membrane protein drug discovery is now an emerging field where the search for physiological mechanisms of regulation and for chemical compounds as modulators of transport activity, present new opportunities for drug development and for new therapies. This Research Topic addresses the latest research advances in membrane transport proteins, stimulating future research on these important protein families.
Download or read book Membrane Transporters written by Qing Yan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of membrane transporters have had great impact on our und- standing human diseases and the design of effective drugs. About 30% of current clinically marketed drugs are targeting membrane transporters or channels. Membrane Transporters: Methods and Protocols provides various practical methodologies for the ongoing research on membrane transporters. To provide readers the most up-to-date information, several emerging fields and methodologies are embraced in this book, including pharmacogenomics, bioin-formatics, and microarray technology. Pharmacogenomics studies of membrane transporters are useful in drug discovery and in predicting drug responses in the clinic. In this volume, the current status of pharma- genomics studies of transporters is reviewed and research methodologies in this field are described. Transporter classification is important in studying the structure and function of membrane transporters and has thus triggered intensive interest in recent years. Membrane Transporters: Methods and Protocols provides a systematic classification of all transmembrane transport proteins found in living org- isms on Earth. This classification system will be helpful for further studies on various aspects of membrane transporters, especially for such large-scale gene expression studies as those employing microarray technologies. Bioinformatics is frequently used in transporter studies and has become indispensable for all kinds of research methods. Commonly used bio- formatics methods, such as databases and tools for sequence analysis and - tif studies, are explained in order to facilitate membrane transporters research. Because of heterogeneous sources and tremendous amounts of data, data in- gration has become one of the most important issues in transporter studies.
Download or read book Channels Carriers and Pumps written by Wilfred D. Stein and published by Elsevier. This book was released on 2014-12-09 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the principles of membrane transport: How molecules and ions move across the cell membrane by simple diffusion and by making use of specialized membrane components (channels, carriers, and pumps). The text emphasizes the quantitative aspects of such movement and its interpretation in terms of transport kinetics. Molecular studies of channels, carriers, and pumps are described in detail as well as structural principles and the fundamental similarities between the various transporters and their evolutionary interrelationships. The regulation of transporters and their role in health and disease are also considered. Provides an introduction to the properties of transport proteins: channels, carriers, and pumps Presents up-to-date information on the structure of transport proteins and on their function and regulation Includes introductions to transport kinetics and to the cloning of genes that code transport proteins Furnishes a link between the experimental basis of the subject and theoretical model building
Download or read book Advances in Biomolecular EPR written by and published by Academic Press. This book was released on 2022-04-22 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more. Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology series - Includes the latest information on Advances in Biomolecular EPR
Download or read book Membrane Transport Mechanism written by Reinhard Krämer and published by Springer Science & Business Media. This book was released on 2014-03-13 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a molecular view of membrane transport by means of numerous biochemical and biophysical techniques. The rapidly growing numbers of atomic structures of transporters in different conformations and the constant progress in bioinformatics have recently added deeper insights. The unifying mechanism of energized solute transport across membranes is assumed to consist of the conformational cycling of a carrier protein to provide access to substrate binding sites from either side of a cellular membrane. Due to the central role of active membrane transport there is considerable interest in deciphering the principles of one of the most fundamental processes in nature: the alternating access mechanism. This book brings together particularly significant structure-function studies on a variety of carrier systems from different transporter families: Glutamate symporters, LeuT-like fold transporters, MFS transporters and SMR (RND) exporters, as well as ABC-type importers. The selected examples impressively demonstrate how the combination of functional analysis, crystallography, investigation of dynamics and computational studies has made it possible to create a conclusive picture or more precisely, “a molecular movie”. Although we are still far from a complete molecular description of the alternating access mechanism, remarkable progress has been made from static snapshots towards membrane transport dynamics.
Download or read book Structure function Relation and Transport Across Gram negative Outer Membrane Channels Investigated by Electrophysiology written by Satya Prathyusha Bhamidimarri and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Movement Disorders Editor s Pick 2021 written by Alberto Albanese and published by Frontiers Media SA. This book was released on 2021-08-19 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Pumps Channels and Transporters written by Ronald J. Clarke and published by John Wiley & Sons. This book was released on 2015-09-16 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes experimental methods for investigating the function of pumps, channels and transporters Covers new emerging analytical methods used to study ion transport membrane proteins such as single-molecule spectroscopy Details a wide range of electrophysiological techniques and spectroscopic methods used to analyze the function of ion channels, ion pumps and transporters Covers state-of-the art analytical methods to study ion pumps, channels, and transporters, and where analytical chemistry can make further contributions
Download or read book Membrane Transport Proteins Advances in Research and Application 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane Transport Proteins: Advances in Research and Application: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Membrane Transport Proteins. The editors have built Membrane Transport Proteins: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Membrane Transport Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Membrane Transport Proteins: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Novel Regulatory Pathways of Protein Channels written by Sheenah Lynn Bryant and published by . This book was released on 2018 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Since the proposal of the fluid mosaic model of a cell membrane, substantial scientific evidence has shown that the cell membrane is not simply an inert structure with the sole role of separating two chemically different environments. The cell membrane dynamically satisfies basic needs, such as water, ion and nutrient transport, without which the cell could not survive. It is a structure which actively participates in a great variety of physiological functions. The activity of the cell membrane is responsible for the contraction of our muscles and information processing in our brain. In order to participate in such a wide range of biological processes, the cell membrane incorporates an extensive variety of protein transporters in its structure. These transporters are highly regulated and contribute to the selective barrier function of the membrane. It is this regulation that enables certain complex physiological functions. The mechanisms of regulation of membrane transporters are obvious in the case of ion channels, which are transmembrane protein transporters facilitating controlled transport of specific ions across the membrane. Their regulation is mediated by specific physical or chemical stimuli, of which voltage, ligands, temperature, light and pressure are most common. However, recent reports indicate that regulation of such transporters may also be achieved by other environmental factors which are not easy to identify in the complex biochemical environment of the cell. Understanding these novel environmental factors and how they modulate the transport across membranes may be a crucial step to better understand the functionality of transmembrane transporters in health and disease. In this respect, the work presented here employs a highly regulated transmembrane transporter, lysenin, which is a pore-forming toxin extracted from red earthworms. Lysenin shares many of the fundamental features of ion channels, such as voltage and ligand regulation. In addition to these features, lysenin accumulates in lipid rafts (which are ubiquitous in animal cells). This model transporter offers opportunities to investigate novel regulatory pathways that are otherwise very difficult to identify in a living cell. In the work presented in this dissertation, I investigated how specific physical and chemical determinants of the membrane and surrounding solution, as well as the gating mechanism itself, may contribute to the emergence of unexpected cellular functionalities. In this endeavor, I showed that increasing the local density of lysenin channels in a target membrane substantially changed the voltage-induced regulation, and that this density can be simply manipulated by altering the membrane's lipid composition. Next, I demonstrated that the macroergic molecule ATP plays an important role in adjusting the conductance of pore-forming transporters and modulates their biological activity. These observations expand the well-established role of ATP as a signaling molecule, which has been proposed and well-studied for the last several decades. Finally, based on experimental observations that lysenin is endowed with molecular memory, I hypothesized a gating mechanism capable of explaining such a novel and unexpected feature. For these investigations, I focused my work on understanding the influence of multivalent cations on lysenin, which are capable of modulating the voltage-induced gating by electrostatic screening of the voltage domain sensor. The proposed gating mechanism, in which the voltage domain sensor moves into the hydrophobic core of the membrane upon gating, is supported by experimental evidence showing that anion binding to the channel lumen presents qualitative and quantitative differences in voltage regulation, as opposed to binding to the voltage domain sensor. Therefore, the work presented here advances our knowledge with respect to how transmembrane transporters are influenced by frequently overlooked environmental factors, and how this may significantly contribute to the achievement of novel physiological functions. This level of understanding may prove crucial for determining potential connections between metabolic pathways and channelopathies that are commonly attributed to genetic defects of ion channels."--Boise State University ScholarWorks.
Download or read book Membrane Transport written by Stephen A. Baldwin and published by OUP Oxford. This book was released on 2000-06-15 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.
Download or read book New Methods and Sensors for Membrane and Cell Volume Research written by and published by Academic Press. This book was released on 2021-12-01 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest. - Describes novel FRET-based membrane sensors - Reviews selected non-optical approaches to membrane structure and dynamics - Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding
Download or read book Membrane Biomechanics written by and published by Academic Press. This book was released on 2020-10-23 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane Biomechanics, Volume 86, the latest release in the Current Topics in Membranes series, highlights new advances in the field, with this new volume presenting interesting chapters on Lipid bilayers: phase behavior and mechanics, Molecular mechanisms of cell membrane structure modification by omega-3 fatty acids, Mechanical properties of magnetoliposomes, Mechanosensitive ion channels and membrane tension, From cell membrane to the nuclear membrane through modulation of cytoskeleton, Endothelial stiffness in dyslipidemia and aging, Vascular smooth muscle stiffness in aging and vascular disease, Mechanobiology of macrovesicle release and activation, Interplay of membrane cholesterol and substrate on vascular smooth muscle mechanics, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Current Topics in Membranes series Includes the latest information on Membrane Biomechanics
Download or read book Structure and Function of GPCRs written by Guillaume Lebon and published by Springer. This book was released on 2019-08-01 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces readers to the latest advances in G protein-coupled receptor (GPCR) biology. It reviews our current understanding of the structural basis of ligand binding and allosteric mechanisms, following a decade of technological breakthroughs. Several examples of structure-based drug discovery are presented, together with the future challenges involved in designing better drugs that target GPCRs. In turn, the book illustrates the important concept of GPCR biased signaling in physiological contexts, and presents fluorescent- and light-based methodologies frequently used to measure GPCR signaling or to trace their dynamics in cells upon ligand activation. Taken together, the chapters provide an essential overview and toolkit for new scientific investigators who plan to develop GPCR projects. All chapters were written by experts in their respective fields, and share valuable insights and powerful methodologies for the GPCR field.
Download or read book Structure Function and Motion in Transmembrane Proteins written by Sarel J. Fleishman and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book VDAC Structure and Function an Up to Date View written by Vito De Pinto and published by Frontiers Media SA. This book was released on 2022-05-03 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:
