Download or read book A Structure function Analysis of the Interaction of the Escherichia Coli NusA Protein with RNA Polymerase the Phage Lambda N Protein and Nut Site RNA written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Structure function Analysis of the Interaction of the Escherichia Coli NusA Protein with RNA Polymerase the Phage Lambda N Protein and Nut Site RNA written by Thien-Fah Mah and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: NusA is an 'E. coli' protein that controls transcription elongation, termination and antitermination. In this thesis, I show that the functions of NusA in transcription are facilitated by interactions with RNA polymerase, RNA and the [lambda] N protein. Use of a series of deletion constructs of NusA allowed me to identify specific regions of NusA involved in specific interactions and in various aspects of NusA function. Genetic evidence suggested that NusA may interact with the 'boxA ' portion of the N-utilization site ('nut' site= ' boxA, interbox,' and 'boxB'). By constructing multiple nucleotide substitutions in the 'nut' site, I showed that the identities of certain nucleotides at the 3' end of ' boxA' and in the 'interbox ' were important for NusA to associate with an N-'nut' site complex. NusA association with RNA in the presence of N is presumably facilitated by its S1 and KH homology regions, two types of RNA-binding domains in NusA. Elimination or mutation of the S1 homology region prevented the association of NusA with an N-' nut' site complex. Using affinity chromatography experiments, I found that RNA polymerase bound equally well to an amino-terminal RNA polymerase-binding region in amino acids 1-137 and a carboxy-terminal RNA polymerase-binding region in amino acids 232-495 of NusA. By contrast, the à subunit of RNA polymerase only bound to the carboxy-terminal RNA polymerase-binding region of NusA. N protein also bound to a carboxy-terminal region of NusA, and both N and à allowed NusA to associate with RNA in a gel mobility shift assay. When the carboxy-terminal region of NusA was deleted in NusA (1-348), the loss of N-binding and Ã-binding ability did not abolish NusA function in termination and antitermination assays. This minimal functional NusA protein retained the KH and S1 homology regions and the amino-terminal RNA polymerase-binding region. Unlike full length NusA (1-495), NusA (1-416) could bind RNA on its own. These observations suggest that the carboxy-terminal region of NusA inhibits RNA binding and that this inhibition can be relieved by interaction with the [lambda] N protein or the à subunit of RNA polymerase.
Download or read book Functional Analysis of the RNA Binding Domains of the E Coli NUSA Protein written by Ying Zhou and published by . This book was released on 2001 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Bacteriophages written by Richard Calendar and published by Oxford University Press. This book was released on 2006 with total page 761 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative, timely, and comprehensively referenced compendium on the bacteriophages explores current views of how viruses infect bacteria. In combination with classical phage molecular genetics, new structural, genomic, and single-molecule technologies have rendered an explosion in our knowledge of phages. Bacteriophages, the most abundant and genetically diverse type of organism in the biosphere, were discovered at the beginning of the 20th century and enjoyed decades of used as anti-bacterial agents before being eclipsed by the antibiotic era. Since 1988, phages have come back into the spotlight as major factors in pathogenesis, bacterial evolution, and ecology. This book reveals their compelling elegence of function and their almost inconceivable diversity.Much of the founding work in molecular biology and structural biology was done on bacteriophages. These are widely used in molecular biology research and in biotechnology, as probes and markers, and in the popular method of assesing gene expression.
Download or read book Structure function Analysis of the Two Largest Subunits of Escherichia Coli RNA Polymerase written by Laura Magdolna Heisler and published by . This book was released on 1994 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Translational Apparatus written by K.H. Nierhaus and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of an international conference held in Berlin, Germany, October 31-November 5, 1992
Download or read book 24th Annual Conference of the German Crystallographic Society March 14 17 2016 Stuttgart Germany written by and published by Walter de Gruyter GmbH & Co KG. This book was released on 2016-03-07 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zeitschrift für Kristallographie. Supplement Volume 36 presents the complete Abstracts of all contributions to the 24th Annual Conference of the German Crystallographic Society in Stuttgart (Germany) 2016: - Plenary Talks - Microsymposia - Poster Session Supplement Series of Zeitschrift für Kristallographie publishes Abstracts of international conferences on the interdisciplinary field of crystallography.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2002 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1985 with total page 1148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1999 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Physiology written by Walid El-Sharoud and published by Springer Science & Business Media. This book was released on 2007-12-07 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of new molecular methodologies in the study of bacterial behavior and cell architecture has enabled new revolutionary insights and discoveries in these areas. This new text presents recent developments in bacterial physiology that are highly relevant to a wide range of readership including those interested in basic and applied knowledge. Its chapters are written by international scientific authorities at the forefront of the subject. The value of this recent knowledge in bacterial physiology is not only restricted to fundamental biology. It also extends to biotechnology and drug-discovery disciplines.
Download or read book Protein protein Interactions Within Escherichia Coli RNA Polymerase Mapped by Artificial Iron dependent Proteases written by Saul Andrew Datwyler and published by . This book was released on 2001 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Chaperones written by Tilman Heise and published by . This book was released on 2020 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones. Chapter 3 is available Open Access under a CC-BY 4.0 license via link.springer.com.
Download or read book Modern Microbial Genetics written by Uldis N. Streips and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: In accordance with its predecessor, the completely revised and expanded Second Edition of Modern Microbial Genetics focuses on how bacteria and bacteriophage arrange and rearrange their genetic material through mutation, evolution, and genetic exchange to take optimal advantage of their environment. The text is divided into three sections: DNA Metabolism, Genetic Response, and Genetic Exchange. The first addresses how DNA replicates, repairs itself, and recombines, as well as how it may be manipulated. The second section is devoted to how microorganisms interact with their environment, including chapters on sporulation and stress shock, and the final section contains the latest information on classic exchange mechanisms such as transformation and conjugation. Chapters include: * Gene Expression and Its Regulation * Single-Stranded DNA Phages * Genetic Tools for Dissecting Motility and Development of Myxococcus xanthus * Molecular Mechanism of Quorum Sensing * Transduction in Gram-Negative Bacteria * Genetic Approaches in Bacteria with No Natural Genetic Systems The editors also cultivate an attention to global regulatory systems throughout the book, elucidating how certain genes and operons in bacteria, defined as regulons, network and cooperate to suit the needs of the bacterial cell. With clear appreciation for the impact of molecular genomics, this completely revised and updated edition proves that Modern Microbial Genetics remains the benchmark text in its field.
Download or read book Fuzziness written by Monika Fuxreiter and published by Springer Science & Business Media. This book was released on 2012-03-07 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detailed characterization of fuzzy interactions will be of central importance for understanding the diverse biological functions of intrinsically disordered proteins in complex eukaryotic signaling networks. In this volume, Peter Tompa and Monika Fuxreiter have assembled a series of papers that address the issue of fuzziness in molecular interactions. These papers provide a broad overview of the phenomenon of fuzziness and provide compelling examples of the central role played by fuzzy interactions in regulation of cellular signaling processes and in viral infectivity. These contributions summarize the current state of knowledge in this new field and will undoubtedly stimulate future research that will further advance our understanding of fuzziness and its role in biomolecular interactions.
Download or read book The Interaction of Escherichia Coli RNA Polymerase with Phage lambda Promoters written by Jung-Hye Roe and published by . This book was released on 1984 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Characterization of the Phage Exclusion and the Local Effect Caused by the Interaction of an E14 Encoded Protein Lit with the Gol Site from Phage T4 Gene 23 written by Yuen-Tsu Nicco Yu and published by . This book was released on 1993 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
