Download or read book A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules written by Randall Mark Stoltenberg and published by Stanford University. This book was released on 2011 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.
Download or read book A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules written by Randall Mark Stoltenberg and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.
Download or read book Single Molecule Tools Part A Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Download or read book Fluids Colloids and Soft Materials written by Alberto Fernandez-Nieves and published by John Wiley & Sons. This book was released on 2016-05-09 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a compilation of self-contained chapters covering a wide range of topics within the broad field of soft condensed matter. Each chapter starts with basic definitions to bring the reader up-to-date on the topic at hand, describing how to use fluid flows to generate soft materials of high value either for applications or for basic research. Coverage includes topics related to colloidal suspensions and soft materials and how they differ in behavior, along with a roadmap for researchers on how to use soft materials to study relevant physics questions related to geometrical frustration.
Download or read book Super Resolution Microscopy for Material Science written by Lorenzo Albertazzi and published by CRC Press. This book was released on 2024-03-26 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical microscopy is one of the most frequently used tools in chemistry and the life sciences. However, its limited resolution hampers the use of optical imaging to many other relevant problems in different disciplines. Super-Resolution Microscopy (SRM) is a new technique that allows the resolution of objects down to a few billionth of meters (nanometers), ten times better than classical microscopes, opening up opportunities to use this tool in new fields. This book describes the theory, principles, and practice of super-resolution microscopy in the field of materials science and nanotechnology. There is a growing interest in the applications of SRM beyond biology as new synthetic materials, such as nanoscale sensors and catalysts, nanostructured materials, functional polymers, and nanoparticles, have nanoscopic features that are challenging to visualize with traditional imaging methods. SRM has the potential to be used to image and understand these cutting-edge man-made objects and guide the design of materials for novel applications. This book is an ideal guide for researchers in the fields of microscopy and materials science and chemistry as well as graduate students studying physics, materials science, biomedical engineering, and chemistry. Key Features: Contains practical guidance on Super-Resolution Microscopy (SRM), an exciting and growing tool that was awarded the Nobel Prize for chemistry in 2014 Provides a new perspective targeting materials science, unlike existing books which target readers in chemistry, life science, and biology Targets students in its core chapters, while offering more advanced material for professionals and researchers in later chapters
Download or read book Recent Advances in Nanofabrication Techniques and Applications written by Bo Cui and published by BoD – Books on Demand. This book was released on 2011-12-02 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology has experienced a rapid growth in the past decade, largely owing to the rapid advances in nanofabrication techniques employed to fabricate nano-devices. Nanofabrication can be divided into two categories: "bottom up" approach using chemical synthesis or self assembly, and "top down" approach using nanolithography, thin film deposition and etching techniques. Both topics are covered, though with a focus on the second category. This book contains twenty nine chapters and aims to provide the fundamentals and recent advances of nanofabrication techniques, as well as its device applications. Most chapters focus on in-depth studies of a particular research field, and are thus targeted for researchers, though some chapters focus on the basics of lithographic techniques accessible for upper year undergraduate students. Divided into five parts, this book covers electron beam, focused ion beam, nanoimprint, deep and extreme UV, X-ray, scanning probe, interference, two-photon, and nanosphere lithography.
Download or read book Cell Membrane Nanodomains written by Alessandra Cambi and published by CRC Press. This book was released on 2014-10-27 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field. Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nanoplatforms. The coverage includes several diffraction-limited imaging techniques that allow for measurements of protein distribution/clustering and membrane curvature in living cells, new fluorescent proteins, novel Laurdan analyses, and the toolbox of labeling possibilities with organic dyes. Since superresolution optical techniques have been crucial to advancing our understanding of cellular structure and protein behavior, the book concludes with a discussion of technologies that are enabling the visualization of lipids, proteins, and other molecular components at unprecedented spatiotemporal resolution. It also explains the ins and outs of the rapidly developing high- or superresolution microscopy field, including new methods and data analysis tools that exclusively pertain to these techniques. This integration of membrane biology and advanced imaging techniques emphasizes the interdisciplinary nature of this exciting field. The array of contributions from leading world experts makes this book a valuable tool for the visualization of signaling nanoplatforms by means of cutting-edge optical microscopy tools.
Download or read book Spectroscopy and Dynamics of Single Molecules written by and published by Elsevier. This book was released on 2019-08-14 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction
Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions
Download or read book Visualizing Chemistry written by National Research Council and published by National Academies Press. This book was released on 2006-06-01 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Download or read book Center for Nanoscale Science and Technology 2010 Biennial Report written by and published by DIANE Publishing. This book was released on with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structured Surfaces as Optical Metamaterials written by Alexei A. Maradudin and published by Cambridge University Press. This book was released on 2011-04-21 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical metamaterials are an exciting new field in optical science. A rapidly developing class of these metamaterials are those that allow the manipulation of volume and surface electromagnetic waves in desirable ways by suitably structuring the surfaces they interact with. They have applications in a variety of fields, such as materials science, photovoltaic technology, imaging and lensing, beam shaping and lasing. Describing techniques and applications, this book is ideal for researchers and professionals working in metamaterials and plasmonics, as well as those just entering this exciting new field. It surveys different types of structured surfaces, their design and fabrication, their unusual optical properties, recent experimental observations and their applications. Each chapter is written by an expert in that area, giving the reader an up-to-date overview of the subject. Both the experimental and theoretical aspects of each topic are presented.
Download or read book Israel Ion Channel and Transporters Meeting 2019 written by Moran Rubinstein and published by Frontiers Media SA. This book was released on 2021-07-21 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Download or read book Single Molecule Spectroscopy in Chemistry Physics and Biology written by Astrid Graslund and published by Springer Science & Business Media. This book was released on 2010-04-17 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
Download or read book Far Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
