Download or read book A Real time PCR Assay for the Rapid Detection and Quantification of Campylobacter Jejuni in Broilers written by Aradom Debretsion and published by . This book was released on 2006 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Development and Optimization of a RT PCR Assay for Rapid Campylobacter Spp Detection and Quantification in Poultry Rinsates written by Aaron Bodie and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Campylobacter is a foodborne pathogen mainly associated with poultry products. Currently, prevalence-based data is used to determine process control during poultry processing. However, this method is not sensitive enough, labor intensive and does not provide information on the level and subsequent risk of Campylobacter contamination. As a result, reliable alternative methods are imperative to detect and quantify Campylobacter contamination throughout the harvest process. Therefore, the goal of this dissertation was to develop and optimize a real-time polymerase chain reaction (RT-PCR) assay for rapid detection and quantification of Campylobacter spp. in poultry rinsates using the United States Department of Agriculture - Food Safety Inspection Services protocol and sampling techniques. The first project was focused on identifying the compositional diversity of pooled colonies enumerated from post-chill poultry carcass samples inoculated with Campylobacter jejuni, coli, and lari on various selective and non-selective media, Campy-Cefex, mCCDA, and TSA; (Chapter 2). The objective of the second study was to generate growth curves for Campylobacter jejuni, coli, and lari under the same growth conditions recommended by the International standard organization (Chapter 3). The last chapter aimed to develop and verify a rapid quantification method for Campylobacter species (CampyQuant[TM]) in post-chill poultry rinsates using the BAX® System Real-Time PCR Assay for Campylobacter (Chapter 4). The results of this dissertation were that BAX® System CampyQuant[TM] assay was able to detect Campylobacter jejuni, coli, and lari after 20 h enrichment and quantify the pre-enrichment concentration in a range from 1.00 to 4.00 Log10 CFU/mL. The study suggests that the CampyQuant[TM] BAX® RT-PCR assay can provide the food industry with a rapid, accurate, and efficient alternative method for Campylobacter spp. enumeration to ensure that process controls are working adequately to provide safe products to consumers.

Download or read book Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques written by Robert E. Levin and published by CRC Press. This book was released on 2009-10-26 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by one of the most prolific and respected researchers in food safety, this volume describes molecular techniques for the detection and discrimination of major infectious bacteria associated with foods. Each chapter deals with a specific organism and techniques applied to that organism. Particular focus is placed on genes associated with pathogenicity used in the polymerase chain reaction (PCR) including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. Methods for extracting microorganisms from complex food matrices andDNA purification techniques are also emphasized.

Download or read book Molecular Detection of Foodborne Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2009-07-28 with total page 905 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases- such as the recent salmonella outbreak linked to various peanut butter products- continue to be reported with alarming frequency.All-Encompassing Guide to Detecti

Download or read book Rapid Detection Characterization and Enumeration of Foodborne Pathogens written by J. Hoorfar and published by American Society for Microbiology Press. This book was released on 2011-04-18 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detect foodborne pathogens early and minimize consumer exposure. • Presents the latest guidelines for fast, easy, cost-effective foodborne pathogen detection. • Enables readers to avoid common pitfalls and choose the most effective and efficient method, assemble the necessary resources, and implement the method seamlessly. • Includes first-hand laboratory experience from more than 85 experts from research centers across the globe.

Download or read book Microbes in the Spotlight written by A. Méndez-Vilas and published by Universal-Publishers. This book was released on 2016-06-28 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbes in the Spotlight: Recent Progress in the Understanding of Beneficial and Harmful Microorganisms contains a selection of papers presented at the VI International Conference on Environmental, Industrial and Applied Microbiology - BioMicroWorld2015 (Barcelona, Spain). This book offers the outcomes of completed and outgoing research works and experiences of several microbiology research groups across the world. The volume is divided into the following sections: --Agricultural and environmental microbiology. Biodeterioration, biodegratation, bioremediation --Food microbiology --Medical microbiology. Antimicrobial agents and chemotherapy. Antimicrobial resistance --Industrial microbiology. Microbial production of high-value products --Biotechnologically relevant enzymes and proteins --Methods and technology development --Microbial physiology Readers will find this book a useful opportunity to keep up with the latest research results, insights and advances in the microbiology field.

Download or read book Rapid Cycle Real Time PCR written by S. Meuer and published by . This book was released on 2001-01-26 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of a Real time PCR Assay for the Detection of Campylobacter Jejuni and Campylobacter Coli written by and published by . This book was released on 2009 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Food Microbiology written by Michael P. Doyle and published by John Wiley & Sons. This book was released on 2019-06-03 with total page 1890 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its introduction in 1997, the purpose of Food Microbiology: Fundamentals and Frontiers has been to serve as an advanced reference that explores the breadth and depth of food microbiology. Thoroughly updated, the new Fifth Edition adds coverage of the ever-expanding tool chest of new and extraordinary molecular methods to address many of the roles that microorganisms play in the production, preservation, and safety of foods. Sections in this valuable reference cover material of special significance to food microbiology such as: stress response mechanisms, spores, and the use of microbiological criteria and indicator organisms commodity-oriented discussion of types of microbial food spoilage and approaches for their control the major foodborne pathogens, including diseases, virulence mechanisms, control measures, and up-to-date details on molecular biology techniques state-of-the-science information on food preservation approaches, including natural antimicrobials and the use of bacteriophages in controlling foodborne pathogens beneficial microbes used in food fermentations and to promote human and animal health updated chapters on current topics such as antimicrobial resistance, predictive microbiology, and risk assessment This respected reference provides up-to-the-minute scientific and technical insights into food production and safety, readily available in one convenient source.

Download or read book Advances in Food Biochemistry written by Fatih Yildiz and published by CRC Press. This book was released on 2009-12-16 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the biochemistry of food is basic to all other research and development in the fields of food science, technology, and nutrition, and the past decade has seen accelerated progress in these areas. Advances in Food Biochemistry provides a unified exploration of foods from a biochemical perspective. Featuring illustrations to elucidate m

Download or read book Waterborne Pathogens written by Helen Bridle and published by Newnes. This book was released on 2013-07-13 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of advanced emerging technologies for the detection of a range of waterborne pathogens. The book will present existing methodology and highlight where improvements can be made, as well as have a strong focus on applications and the ways in which new technology could be applied in water management. Additionally, it addresses issues of sample preparation (from sampling to concentration and enrichment), a key stage in any detection protocol. Covers the gap of specific sound methods of pathogen detection by fulfulling the need for a concept book on the novel technologies for pathogen detection in water Presents all cutting-edge technologies for pathogen detection in water as well as recent emerging technologies Addresses all three types of pathogens; this combined knowledge helps to understand all potential pathogens in water

Download or read book Detection of Non Amplified Genomic DNA written by Giuseppe Spoto and published by Springer Science & Business Media. This book was released on 2012-07-06 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.

Download or read book Advanced Technologies For Meat Processing written by Leo M.L. Nollet and published by CRC Press. This book was released on 2006-03-21 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the meat industry has incorporated important technological advances that, to this point, have not been addressed in a single source. Comprehensive and authoritative, Advanced Technologies for Meat Processing presents developments concerning the quality, analysis, and processing of meat and meat products. Co-Edited by Fidel Toldra - Recipient of the 2010 Distinguished Research Award from the American Meat Science Association Featuring contributions from a panel of international experts, the book details technologies used in the meat processing chain. It describes important processing methodologies such as gene technology, automation, irradiation, hot boning, high pressure, vacuum-salting, enzymes, starters, and bacteriocins. The book begins by exploring various production systems that include the use of modern biotechnology, automation in slaughterhouses, and rapid non-destructive on-line detection systems. It proceeds to describe different new technologies such as decontamination, high pressure processing, and fat reduction. The book then examines functional meat compounds such as peptides and antioxidants and the processing of nitrate-free products and dry-cured meat products. It also discusses bacteriocins that fight against meat-borne pathogens and the latest developments in bacterial starters for improved flavor in fermented meats. It concludes with a discussion of packaging systems of the final products.

Download or read book Campylobacter in Poultry Physiology Genetics and Detection Methods written by Steven C. Ricke and published by Frontiers Media SA. This book was released on 2022-11-29 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Food Policy Control and Research written by Arthur P. Riley and published by Nova Publishers. This book was released on 2005 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new book examines in detail food policy, control and research.

Download or read book Advances in Food Biotechnology written by Ravishankar Rai V and published by John Wiley & Sons. This book was released on 2015-12-21 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of biotechnology in the food sciences has led to an increase in food production and enhanced the quality and safety of food. Food biotechnology is a dynamic field and the continual progress and advances have not only dealt effectively with issues related to food security but also augmented the nutritional and health aspects of food. Advances in Food Biotechnology provides an overview of the latest development in food biotechnology as it relates to safety, quality and security. The seven sections of the book are multidisciplinary and cover the following topics: GMOs and food security issues Applications of enzymes in food processing Fermentation technology Functional food and nutraceuticals Valorization of food waste Detection and control of foodborne pathogens Emerging techniques in food processing Bringing together experts drawn from around the world, the book is a comprehensive reference in the most progressive field of food science and will be of interest to professionals, scientists and academics in the food and biotech industries. The book will be highly resourceful to governmental research and regulatory agencies and those who are studying and teaching food biotechnology.

Download or read book PCR Methods in Foods written by John Maurer and published by Springer Science & Business Media. This book was released on 2006-11-22 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will introduce non-molecular biologists to diagnostic PCR-based te- nologies for the detection of pathogens in foods. By the conclusion of this book, the reader should be able to: 1) understand the principles behind PCR including real-time; 2) know the basics involved in the design, optimization, and imp- mentation of PCR in food microbiology lab setting; 3) interpret results; 4) know limitations and strengths of PCR; and 5) understand the basic principles behind a new fledgling technology, microarrays and its potential applications in food microbiology. This book will provide readers with the latest information on PCR and microarray based tests and their application towards the detection of bacterial, protozoal and viral pathogens in foods. Figures, charts, and tables will be used, where appropriate, to help illustrate concepts or provide the reader with useful information or resources as an important starting point in bringing molecular diagnostics into the food microbiology lab. This book is not designed to be a “cookbook”PCR manual with recipes and step-by-step instructions but rather serve as a primer or resource book for students, faculty, and other professionals interested in molecular biology and its integration into food safety. v Table of Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Chapter 1. PCR Basics Amanda Fairchild, M. S. , Margie D. Lee DVM, Ph. D. , and John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. The Mythology of PCR: A Warning to the Wise John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Chapter 3.